(Circular) (Six-base) MAPSORT of: pFN21AE5509.seq Check: 1135 from: 1 to: 5995 pFN21AE5509 5995 bp With 182 enzymes: SgfI * January 30, 2014 17:21 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3216 3805 278 Size: 53 83 186 936 1680 589 2468 Fragments arranged by size: 2468 1680 936 589 186 83 53 AccI GT'mk_AC Cuts at: 1937 3338 1937 Size: 1401 4594 Acc65I G'GTAC_C Cuts at: 3316 3316 Size: 5995 AclI AA'CG_TT Cuts at: 1563 2685 4122 4495 1563 Size: 1122 1437 373 3063 Fragments arranged by size: 3063 1437 1122 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4058 5442 5710 1632 Size: 2426 1384 268 1917 Fragments arranged by size: 2426 1917 1384 268 AfeI AGC'GCT Cuts at: 3260 3260 Size: 5995 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5798 AflIII A'CryG_T Cuts at: 1203 2997 4894 5871 1203 Size: 1794 1897 977 1327 Fragments arranged by size: 1897 1794 1327 977 AgeI A'CCGG_T Cuts at: 4826 4826 Size: 5995 AhdI GACnn_n'nnGTC Cuts at: 2061 2438 4724 2061 Size: 377 2286 3332 Fragments arranged by size: 3332 2286 377 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5995 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2490 1184 Size: 1306 4689 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2068 2100 2068 Size: 32 5963 AlwNI CAG_nnn'CTG Cuts at: 2321 3738 5310 2321 Size: 1417 1572 3006 Fragments arranged by size: 3006 1572 1417 ApaI G_GGCC'C Cuts at: 3174 3174 Size: 5995 ApaLI G'TGCA_C Cuts at: 2199 4052 5208 2199 Size: 1853 1156 2986 Fragments arranged by size: 2986 1853 1156 ApoI r'AATT_y Cuts at: 1493 3304 3530 3682 5791 1493 Size: 1811 226 152 2109 1697 Fragments arranged by size: 2109 1811 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 3024 4549 160 Size: 872 1992 1525 1606 Fragments arranged by size: 1992 1606 1525 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5995 AvaI C'yCGr_G Cuts at: 1870 1943 3165 3320 1870 Size: 73 1222 155 4545 Fragments arranged by size: 4545 1222 155 73 BamHI G'GATC_C Cuts at: 3184 3325 3692 3184 Size: 141 367 5487 Fragments arranged by size: 5487 367 141 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3316 4771 618 Size: 325 217 627 1529 1455 1842 Fragments arranged by size: 1842 1529 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3063 3174 3314 1391 Size: 1672 111 140 4072 Fragments arranged by size: 4072 1672 140 111 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2868 3245 928 Size: 971 969 377 3678 Fragments arranged by size: 3678 971 969 377 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1999 2033 4186 4220 1903 Size: 34 62 34 2153 34 3678 Fragments arranged by size: 3678 2153 62 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2661 3887 5097 2661 Size: 1226 1210 3559 Fragments arranged by size: 3559 1226 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5995 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4606 136 Size: 107 122 71 631 605 2934 1525 Fragments arranged by size: 2934 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5990 5990 Size: 5995 BlpI GC'TnA_GC Cuts at: 3032 3412 3032 Size: 380 5615 Bme1580I G_kGCm'C Cuts at: 1790 2203 3174 4056 5212 1790 Size: 413 971 882 1156 2573 Fragments arranged by size: 2573 1156 971 882 413 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5995 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2168 2226 3235 Size: 416 53 83 399 24 751 58 1009 Cuts at: 3235 3726 4684 442 Size: 491 958 1753 Fragments arranged by size: 1753 1009 958 751 491 416 399 83 58 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5995 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2349 3049 4655 1239 Size: 1110 700 1606 2579 Fragments arranged by size: 2579 1606 1110 700 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2212 2693 3196 4120 4985 5283 2212 Size: 481 503 924 865 298 2924 Fragments arranged by size: 2924 924 865 503 481 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3016 4658 882 Size: 617 1517 1642 2219 Fragments arranged by size: 2219 1642 1517 617 BsaAI yAC'GTr Cuts at: 493 1204 3786 493 Size: 711 2582 2702 Fragments arranged by size: 2702 2582 711 BsaBI GATnn'nnATC Cuts at: 5989 5989 Size: 5995 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3213 3802 4184 4819 Size: 53 83 186 936 1680 589 382 635 Cuts at: 4819 275 Size: 1451 Fragments arranged by size: 1680 1451 936 635 589 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4428 4826 5100 5247 5658 4428 Size: 398 274 147 411 4765 Fragments arranged by size: 4765 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2068 2098 1178 Size: 30 165 30 665 30 5075 Fragments arranged by size: 5075 665 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5995 BseYI C'CCAG_C Cuts at: 5198 5694 5198 Size: 496 5499 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2486 3228 1163 Size: 573 750 742 3930 Fragments arranged by size: 3930 750 742 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2013 3445 4206 5234 1540 Size: 275 186 12 1432 761 1028 2301 Fragments arranged by size: 2301 1432 1028 761 275 186 12 BsiHKAI G_wGCw'C Cuts at: 2203 4056 4141 5212 2203 Size: 1853 85 1071 2986 Fragments arranged by size: 2986 1853 1071 85 BsmI GAATG_Cn' Cuts at: 3506 3599 3506 Size: 93 5902 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2203 3063 3174 3314 4056 4141 5212 Size: 399 413 860 111 140 742 85 1071 Cuts at: 5212 1391 Size: 2174 Fragments arranged by size: 2174 1071 860 742 413 399 140 111 85 BspEI T'CCGG_A Cuts at: 5658 5658 Size: 5995 BspHI T'CATG_A Cuts at: 3884 5614 3884 Size: 1730 4265 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2051 2756 3350 844 Size: 1207 705 594 3489 Fragments arranged by size: 3489 1207 705 594 BsrBI CCG'CTC Cuts at: 1394 1823 3882 1394 Size: 429 2059 3507 Fragments arranged by size: 3507 2059 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2146 2472 4490 4664 65 Size: 1183 898 326 2018 174 1396 Fragments arranged by size: 2018 1396 1183 898 326 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2976 4639 4826 1700 Size: 110 1166 1663 187 2869 Fragments arranged by size: 2869 1663 1166 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5995 BssHII G'CGCG_C Cuts at: 1925 2020 1925 Size: 95 5900 BssSI C'ACGA_G Cuts at: 4055 5067 4055 Size: 1012 4983 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5995 BstBI TT'CG_AA Cuts at: 4799 4799 Size: 5995 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5995 BstYI r'GATC_y Cuts at: 1972 2377 3184 3325 3692 4077 4094 5546 5632 Size: 405 807 141 367 385 17 1452 86 Cuts at: 5632 5644 5990 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 807 405 385 367 346 141 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2013 3055 3291 513 Size: 552 939 9 1042 236 3217 Fragments arranged by size: 3217 1042 939 552 236 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5256 Fragments arranged by size: 5256 683 56 BtsI GCAGTG_nn' Cuts at: 2087 2201 2469 3511 4305 4325 2087 Size: 114 268 1042 794 20 3757 Fragments arranged by size: 3757 1042 794 268 114 20 ClaI AT'CG_AT Cuts at: 1554 2723 1554 Size: 1169 4826 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5960 DraI TTT'AAA Cuts at: 2899 3299 2899 Size: 400 5595 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5002 809 Size: 507 3686 1802 Fragments arranged by size: 3686 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2143 3442 4331 Size: 54 1425 50 275 18 313 1299 889 Cuts at: 4331 5960 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1299 889 313 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3442 1537 Size: 275 1630 4090 Fragments arranged by size: 4090 1630 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2120 2338 2487 2529 3056 3275 3925 1353 Size: 767 218 149 42 527 219 650 3423 Fragments arranged by size: 3423 767 650 527 219 218 149 42 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4582 4956 5102 387 Size: 1064 138 2993 374 146 1280 Fragments arranged by size: 2993 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2349 3049 4058 4655 5442 5710 1239 Size: 393 717 700 1009 597 787 268 1524 Fragments arranged by size: 1524 1009 787 717 700 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 3041 3041 Size: 5995 EcoO109I rG'GnC_Cy Cuts at: 3046 3103 3170 3171 3046 Size: 57 67 1 5870 Fragments arranged by size: 5870 67 57 1 EcoRI G'AATT_C Cuts at: 3304 3682 3304 Size: 378 5617 FspI TGC'GCA Cuts at: 4501 4501 Size: 5995 HaeII r_GCGC'y Cuts at: 1678 2310 3262 5142 1678 Size: 632 952 1880 2531 Fragments arranged by size: 2531 1880 952 632 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2047 2079 2424 2456 3169 Size: 32 135 32 475 32 345 32 713 Cuts at: 3169 3201 4636 4668 4710 4742 1373 Size: 32 1435 32 42 32 2626 Fragments arranged by size: 2626 1435 713 475 345 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3339 3585 669 Size: 988 281 1401 246 3079 Fragments arranged by size: 3079 1401 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5995 HpaI GTT'AAC Cuts at: 3585 3585 Size: 5995 KpnI G_GTAC'C Cuts at: 3320 3320 Size: 5995 MfeI C'AATT_G Cuts at: 3594 3594 Size: 5995 MluI A'CGCG_T Cuts at: 5871 5871 Size: 5995 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5109 5293 1305 Size: 3804 184 2007 Fragments arranged by size: 3804 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2145 10 Size: 54 1425 343 313 3860 Fragments arranged by size: 3860 1425 343 313 54 MslI CAynn'nnrTG Cuts at: 518 1166 3026 3953 4312 4471 518 Size: 648 1860 927 359 159 2042 Fragments arranged by size: 2042 1860 927 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2015 2949 3088 3411 4088 5236 5481 1606 Size: 409 934 139 323 677 1148 245 2120 Fragments arranged by size: 2120 1148 934 677 409 323 245 139 NaeI GCC'GGC Cuts at: 2978 2978 Size: 5995 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4504 Fragments arranged by size: 4504 939 552 NdeI CA'TA_TG Cuts at: 387 2647 387 Size: 2260 3735 NgoMIV G'CCGG_C Cuts at: 2976 2976 Size: 5995 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5995 NotI GC'GGCC_GC Cuts at: 3442 3442 Size: 5995 NspI r_CATG'y Cuts at: 1132 1229 3001 3353 4898 1132 Size: 97 1772 352 1545 2229 Fragments arranged by size: 2229 1772 1545 352 97 PasI CC'CwG_GG Cuts at: 1348 3043 1348 Size: 1695 4300 PciI A'CATG_T Cuts at: 2997 4894 2997 Size: 1897 4098 PflMI CCAn_nnn'nTGG Cuts at: 1108 3115 1108 Size: 2007 3988 PfoI T'CCnGG_A Cuts at: 3123 3123 Size: 5995 PmeI GTTT'AAAC Cuts at: 3299 3299 Size: 5995 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2068 2100 4064 4096 5577 5609 2068 Size: 32 1964 32 1481 32 2454 Fragments arranged by size: 2454 1964 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2628 1613 Size: 1015 4980 PsiI TTA'TAA Cuts at: 3565 3565 Size: 5995 PspOMI G'GGCC_C Cuts at: 3170 3170 Size: 5995 PstI C_TGCA'G Cuts at: 830 2208 2290 2767 3347 830 Size: 1378 82 477 580 3478 Fragments arranged by size: 3478 1378 580 477 82 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5995 PvuII CAG'CTG Cuts at: 3088 3088 Size: 5995 SacII CC_GC'GG Cuts at: 2016 2016 Size: 5995 SalI G'TCGA_C Cuts at: 1936 3337 1936 Size: 1401 4594 SapI GCTCTTCn'nnn_ Cuts at: 2487 2487 Size: 5995 SbfI CC_TGCA'GG Cuts at: 3347 3347 Size: 5995 ScaI AGT'ACT Cuts at: 4243 5955 4243 Size: 1712 4283 SfcI C'TryA_G Cuts at: 826 1045 2204 2286 2763 3343 4478 5159 5350 Size: 219 1159 82 477 580 1135 681 191 Cuts at: 5350 826 Size: 1471 Fragments arranged by size: 1471 1159 1135 681 580 477 219 191 82 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5995 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5995 SmaI CCC'GGG Cuts at: 1872 3167 3322 1872 Size: 1295 155 4545 Fragments arranged by size: 4545 1295 155 SmlI C'TyrA_G Cuts at: 820 1017 1943 2191 2672 3175 4099 5000 5262 Size: 197 926 248 481 503 924 901 262 Cuts at: 5262 820 Size: 1553 Fragments arranged by size: 1553 926 924 901 503 481 262 248 197 SnaBI TAC'GTA Cuts at: 493 3786 493 Size: 3293 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5995 SphI G_CATG'C Cuts at: 1132 3353 1132 Size: 2221 3774 SspI AAT'ATT Cuts at: 5 52 2874 3919 5 Size: 47 2822 1045 2081 Fragments arranged by size: 2822 2081 1045 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5995 StyI C'CwwG_G Cuts at: 513 1065 2004 3006 3434 513 Size: 552 939 1002 428 3074 Fragments arranged by size: 3074 1002 939 552 428 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2189 2559 3056 4022 4039 4192 4377 Size: 1255 609 370 497 966 17 153 185 Cuts at: 4377 4822 325 Size: 445 1498 Fragments arranged by size: 1498 1255 966 609 497 445 370 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4241 5953 96 Size: 274 80 33 51 1443 2264 1712 138 Fragments arranged by size: 2264 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5955 5987 5955 Size: 32 5963 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5995 XbaI T'CTAG_A Cuts at: 3331 3331 Size: 5995 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5995 XmaI C'CCGG_G Cuts at: 1870 3165 3320 1870 Size: 1295 155 4545 Fragments arranged by size: 4545 1295 155 XmnI GAAnn'nnTTC Cuts at: 4124 4124 Size: 5995 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3214 3803 276 Size: 53 83 186 936 1680 589 2468 Fragments arranged by size: 2468 1680 936 589 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SanDI SexAI SfiI SfoI SrfI SwaI XcmI