(Linear) (Six-base) MAPSORT of: KIEE5509.seq Check: 7096 from: 1 to: 1290 KIEE5509.orf With 182 enzymes: SgfI * January 29, 2014 12:03 .. AatII G_ACGT'C Cuts at: 0 1211 1290 Size: 1211 79 AclI AA'CG_TT Cuts at: 0 680 1290 Size: 680 610 AfeI AGC'GCT Cuts at: 0 1255 1290 Size: 1255 35 AflIII A'CryG_T Cuts at: 0 992 1290 Size: 992 298 AhdI GACnn_n'nnGTC Cuts at: 0 56 433 1290 Size: 56 377 857 Fragments arranged by size: 857 377 56 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 485 1290 Size: 485 805 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 63 95 1290 Size: 63 32 1195 Fragments arranged by size: 1195 63 32 AlwNI CAG_nnn'CTG Cuts at: 0 316 1290 Size: 316 974 ApaI G_GGCC'C Cuts at: 0 1169 1290 Size: 1169 121 ApaLI G'TGCA_C Cuts at: 0 194 1290 Size: 194 1096 AseI AT'TA_AT Cuts at: 0 1019 1290 Size: 1019 271 AvaI C'yCGr_G Cuts at: 0 1160 1290 Size: 1160 130 BamHI G'GATC_C Cuts at: 0 1179 1290 Size: 1179 111 BanII G_rGCy'C Cuts at: 0 1058 1169 1290 Size: 1058 111 121 Fragments arranged by size: 1058 121 111 BbsI GAAGACnn'nnnn_ Cuts at: 0 863 1240 1290 Size: 863 377 50 Fragments arranged by size: 863 377 50 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 28 1290 Size: 28 1262 BciVI GTATCCnnnnn_n' Cuts at: 0 656 1290 Size: 656 634 BlpI GC'TnA_GC Cuts at: 0 1027 1290 Size: 1027 263 Bme1580I G_kGCm'C Cuts at: 0 198 1169 1290 Size: 198 971 121 Fragments arranged by size: 971 198 121 BmrI ACTGGGnnnn_n' Cuts at: 0 163 221 1230 1290 Size: 163 58 1009 60 Fragments arranged by size: 1009 163 60 58 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 344 1044 1290 Size: 344 700 246 Fragments arranged by size: 700 344 246 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 207 688 1191 1290 Size: 207 481 503 99 Fragments arranged by size: 503 481 207 99 BsaI GGTCTCn'nnnn_ Cuts at: 0 1011 1290 Size: 1011 279 BsaHI Gr'CG_yC Cuts at: 0 1208 1290 Size: 1208 82 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 63 93 1290 Size: 63 30 1197 Fragments arranged by size: 1197 63 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 481 1223 1290 Size: 481 742 67 Fragments arranged by size: 742 481 67 BsiEI CG_ry'CG Cuts at: 0 8 1290 Size: 8 1282 BsiHKAI G_wGCw'C Cuts at: 0 198 1290 Size: 198 1092 Bsp1286I G_dGCh'C Cuts at: 0 198 1058 1169 1290 Size: 198 860 111 121 Fragments arranged by size: 860 198 121 111 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 46 751 1290 Size: 46 705 539 Fragments arranged by size: 705 539 46 BsrDI GCAATG_nn' Cuts at: 0 141 467 1290 Size: 141 326 823 Fragments arranged by size: 823 326 141 BsrFI r'CCGG_y Cuts at: 0 971 1290 Size: 971 319 BssHII G'CGCG_C Cuts at: 0 15 1290 Size: 15 1275 BstYI r'GATC_y Cuts at: 0 372 1179 1290 Size: 372 807 111 Fragments arranged by size: 807 372 111 BtgI C'CryG_G Cuts at: 0 8 1050 1290 Size: 8 1042 240 Fragments arranged by size: 1042 240 8 BtsI GCAGTG_nn' Cuts at: 0 82 196 464 1290 Size: 82 114 268 826 Fragments arranged by size: 826 268 114 82 ClaI AT'CG_AT Cuts at: 0 718 1290 Size: 718 572 DraI TTT'AAA Cuts at: 0 894 1290 Size: 894 396 EaeI y'GGCC_r Cuts at: 0 138 1290 Size: 138 1152 EarI CTCTTCn'nnn_ Cuts at: 0 115 333 482 524 1051 1270 1290 Size: 115 218 149 42 527 219 20 Fragments arranged by size: 527 219 218 149 115 42 20 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 344 1044 1290 Size: 344 700 246 Fragments arranged by size: 700 344 246 EcoNI CCTnn'n_nnAGG Cuts at: 0 1036 1290 Size: 1036 254 EcoO109I rG'GnC_Cy Cuts at: 0 1041 1098 1165 1166 1290 Size: 1041 57 67 1 124 Fragments arranged by size: 1041 124 67 57 1 HaeII r_GCGC'y Cuts at: 0 305 1257 1290 Size: 305 952 33 Fragments arranged by size: 952 305 33 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 42 74 419 451 1164 1196 1290 Size: 42 32 345 32 713 32 94 Fragments arranged by size: 713 345 94 42 32 32 32 MscI TGG'CCA Cuts at: 0 140 1290 Size: 140 1150 MslI CAynn'nnrTG Cuts at: 0 1021 1290 Size: 1021 269 MspA1I CmG'CkG Cuts at: 0 10 944 1083 1290 Size: 10 934 139 207 Fragments arranged by size: 934 207 139 10 NaeI GCC'GGC Cuts at: 0 973 1290 Size: 973 317 NdeI CA'TA_TG Cuts at: 0 642 1290 Size: 642 648 NgoMIV G'CCGG_C Cuts at: 0 971 1290 Size: 971 319 NspI r_CATG'y Cuts at: 0 996 1290 Size: 996 294 PasI CC'CwG_GG Cuts at: 0 1038 1290 Size: 1038 252 PciI A'CATG_T Cuts at: 0 992 1290 Size: 992 298 PflMI CCAn_nnn'nTGG Cuts at: 0 1110 1290 Size: 1110 180 PfoI T'CCnGG_A Cuts at: 0 1118 1290 Size: 1118 172 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 63 95 1290 Size: 63 32 1195 Fragments arranged by size: 1195 63 32 PshAI GACnn'nnGTC Cuts at: 0 623 1290 Size: 623 667 PspOMI G'GGCC_C Cuts at: 0 1165 1290 Size: 1165 125 PstI C_TGCA'G Cuts at: 0 203 285 762 1290 Size: 203 82 477 528 Fragments arranged by size: 528 477 203 82 PvuII CAG'CTG Cuts at: 0 1083 1290 Size: 1083 207 SacII CC_GC'GG Cuts at: 0 11 1290 Size: 11 1279 SapI GCTCTTCn'nnn_ Cuts at: 0 482 1290 Size: 482 808 SfcI C'TryA_G Cuts at: 0 199 281 758 1290 Size: 199 82 477 532 Fragments arranged by size: 532 477 199 82 SmaI CCC'GGG Cuts at: 0 1162 1290 Size: 1162 128 SmlI C'TyrA_G Cuts at: 0 186 667 1170 1290 Size: 186 481 503 120 Fragments arranged by size: 503 481 186 120 SspI AAT'ATT Cuts at: 0 869 1290 Size: 869 421 StyI C'CwwG_G Cuts at: 0 1001 1290 Size: 1001 289 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 184 554 1051 1290 Size: 184 370 497 239 Fragments arranged by size: 497 370 239 184 XmaI C'CCGG_G Cuts at: 0 1160 1290 Size: 1160 130 ZraI GAC'GTC Cuts at: 0 1209 1290 Size: 1209 81 Enzymes that do cut: AatII AclI AfeI AflIII AhdI AlfI AloI AlwNI ApaI ApaLI AseI AvaI BamHI BanII BbsI BcgI BciVI BlpI Bme1580I BmrI BpmI BpuEI BsaI BsaHI BsaXI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrDI BsrFI BssHII BstYI BtgI BtsI ClaI DraI EaeI EarI Eco57MI EcoNI EcoO109I HaeII Hin4I MscI MslI MspA1I NaeI NdeI NgoMIV NspI PasI PciI PflMI PfoI PpiI PshAI PspOMI PstI PvuII SacII SapI SfcI SmaI SmlI SspI StyI TaqII XmaI ZraI Enzymes that do not cut: AarI AccI Acc65I AcuI AflII AgeI AleI ApoI AscI AsiSI AvrII BaeI BanI BbeI BbvCI BclI BfrBI BglI BglII BmgBI BmtI BplI Bpu10I BsaAI BsaBI BsaWI BseRI BseYI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrGI BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI CspCI DraIII DrdI EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MmeI NarI NcoI NheI NotI NruI NsiI PacI PmeI PmlI PpuMI PsiI PspXI PsrI PvuI RsrII SacI SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI StuI SwaI TatI TstI Tth111I XbaI XcmI XhoI XmnI