################################## # NO CUT ENZYMES ( 125 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AfaI AflII AgeI Alw44I ApaLI AscI AseI AsnI Asp718I AspI AviII AvrII BalI BbrPI BbvCI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BsrGI BssHII BssSI Bst1107I BstBI BstZ17I CelII ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI EarI EclXI Eco52I EcoNI EcoRV EcoT22I FbaI FseI FspI HincII HindII HpaI KpnI Ksp632I KspI MaeII MamI MfeI MluI MluNI MroI MscI MseI MunI NcoI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccII 202 CGCG CG^CG AflIII 1555 ACATGT A^C[GA][CT]GT AhdI 34 GACGCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 858 CCTGAGG CC^T[ACGT]AGG Aor51HI 161 AGCGCT AGC^GCT Asp700I 1230 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 34 GACGCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 137 CTCGGG C^[CT]CG[GA]G AvaII 647 GGTCC G^G[AT]CC BamHI 547 GGATCC G^GATCC BanI 414 GGCGCC G^G[CT][GA]CC BbeI 414 GGCGCC GGCGC^C BfaI 5 CTAG C^TAG BglI 100 GCCTTGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BseRI 364 GAGGAG GAGGAG(10/8) BsoBI 137 CTCGGG C^[CT]CG[GA]G BspLU11I 1555 ACATGT A^CATGT BsrBI 357 GAGCGG CCGCTC(-3/-3) BstAPI 444 GCAAGAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 433 GGTGACC G^GT[ACGT]ACC BstPI 433 GGTGACC G^GT[ACGT]ACC BstUI 202 CGCG CG^CG Bsu36I 858 CCTGAGG CC^T[ACGT]AGG Eam1105I 34 GACGCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 161 AGCGCT AGC^GCT Eco81I 858 CCTGAGG CC^T[ACGT]AGG EcoO65I 433 GGTGACC G^GT[ACGT]ACC EcoRI 1329 GAATTC G^AATTC EcoT14I 774 CCTTGG C^C[AT][AT]GG HgaI 34 GACGC GACGC(5/10) HindIII 823 AAGCTT A^AGCTT KasI 414 GGCGCC G^GCGCC MaeI 5 CTAG C^TAG MvnI 202 CGCG CG^CG NarI 414 GGCGCC GG^CGCC NdeI 1124 CATATG CA^TATG PpuMI 646 GGGTCCC [GA]G^G[AT]CC[CT] SacI 1077 GAGCTC GAGCT^C SfoI 414 GGCGCC GGC^GCC SmlI 485 CTCAAG C^T[CT][GA]AG StuI 999 AGGCCT AGG^CCT StyI 774 CCTTGG C^C[AT][AT]GG TaqI 1575 TCGA T^CGA TthHB8I 1575 TCGA T^CGA XmnI 1230 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AcsI 1271 AAATTC [GA]^AATT[CT] 1329 GAATTC AcyI 34 GACGCC G[GA]^CG[CT]C 414 GGCGCC ApaI 141 GGGCCC GGGCC^C 195 GGGCCC ApoI 1271 AAATTC [GA]^AATT[CT] 1329 GAATTC BcgI 223 CGAGAACTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 955 CGACATTATTGC BcnI 183 CCCGG CC^[GC]GG 316 CCGGG BsaHI 34 GACGCC G[GA]^CG[CT]C 414 GGCGCC BsaI 491 GGTCTC GGTCTC(1/5) 768 GGTCTC BsmFI 803 GGGAC GGGAC(10/14) 648 GTCCC BspMI 420 GCAGGT ACCTGC(4/8) 914 GCAGGT BsrDI 870 CATTGC GCAATG(2/0) 619 GCAATG BsrFI 215 GCCGGC [GA]^CCGG[CT] 411 GCCGGC BstXI 576 CCAGGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 966 CCATGAATGTGG BstYI 272 AGATCC [GA]^GATC[CT] 547 GGATCC CfoI 162 GCGC GCG^C 415 GCGC Cfr10I 215 GCCGGC [GA]^CCGG[CT] 411 GCCGGC DpnII 273 GATC ^GATC 548 GATC HaeII 161 AGCGCT [GA]GCGC^[CT] 414 GGCGCC HhaI 162 GCGC GCG^C 415 GCGC Hin1I 34 GACGCC G[GA]^CG[CT]C 414 GGCGCC HinP1I 162 GCGC G^CGC 415 GCGC MboI 273 GATC ^GATC 548 GATC MflI 272 AGATCC [GA]^GATC[CT] 547 GGATCC MspA1I 400 CAGCTG C[AC]G^C[GT]G 706 CAGCTG NaeI 215 GCCGGC GCC^GGC 411 GCCGGC NciI 183 CCCGG CC^[GC]GG 316 CCGGG NdeII 273 GATC ^GATC 548 GATC NgoMIV 215 GCCGGC G^CCGGC 411 GCCGGC NspI 888 ACATGC [GA]CATG^[CT] 1555 ACATGT PleI 1103 GAGTC GAGTC(4/5) 1396 GAGTC PvuII 400 CAGCTG CAG^CTG 706 CAGCTG Sau3AI 273 GATC ^GATC 548 GATC Tsp509I 1272 AATT ^AATT 1330 AATT XhoII 272 AGATCC [GA]^GATC[CT] 547 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 54 enzymes) # ######################################### AciI AluI AlwI AlwNI AspHI BanII BbsI BbvI BmyI BpmI BpuAI BsaJI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DraII EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeIII MboII MnlI MslI MspI MvaI MwoI NlaIII NlaIV PstI Sau96I ScrFI SfaNI SfcI TfiI TseI Tsp45I TspRI