(Circular) (Six-base) MAPSORT of: pFN21AB7847.seq Check: 9186 from: 1 to: 6340 pFN21AB7847 6340 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4150 278 Size: 53 83 186 936 2614 2468 Fragments arranged by size: 2614 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3683 1937 Size: 1746 4594 Acc65I G'GTAC_C Cuts at: 3661 3661 Size: 6340 AclI AA'CG_TT Cuts at: 1563 4467 4840 1563 Size: 2904 373 3063 Fragments arranged by size: 3063 2904 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3142 3226 4403 5787 6055 1632 Size: 1510 84 1177 1384 268 1917 Fragments arranged by size: 1917 1510 1384 1177 268 84 AfeI AGC'GCT Cuts at: 2168 2168 Size: 6340 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6143 AflIII A'CryG_T Cuts at: 1203 3560 5239 6216 1203 Size: 2357 1679 977 1327 Fragments arranged by size: 2357 1679 1327 977 AgeI A'CCGG_T Cuts at: 5171 5171 Size: 6340 AhdI GACnn_n'nnGTC Cuts at: 2044 5069 2044 Size: 3025 3315 AleI CACnn'nnGTG Cuts at: 1166 3033 3117 3534 1166 Size: 1867 84 417 3972 Fragments arranged by size: 3972 1867 417 84 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2828 2958 1184 Size: 1644 130 4566 Fragments arranged by size: 4566 1644 130 AlwNI CAG_nnn'CTG Cuts at: 2281 2308 2903 4083 5655 2281 Size: 27 595 1180 1572 2966 Fragments arranged by size: 2966 1572 1180 595 27 ApaI G_GGCC'C Cuts at: 2150 2204 2150 Size: 54 6286 ApaLI G'TGCA_C Cuts at: 4397 5553 4397 Size: 1156 5184 ApoI r'AATT_y Cuts at: 1493 3276 3334 3649 3875 4027 6136 1493 Size: 1783 58 315 226 152 2109 1697 Fragments arranged by size: 2109 1783 1697 315 226 152 58 AseI AT'TA_AT Cuts at: 160 1032 4894 160 Size: 872 3862 1606 Fragments arranged by size: 3862 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6340 AvaI C'yCGr_G Cuts at: 1870 1943 2142 3665 1870 Size: 73 199 1523 4545 Fragments arranged by size: 4545 1523 199 73 BamHI G'GATC_C Cuts at: 2552 3670 4037 2552 Size: 1118 367 4855 Fragments arranged by size: 4855 1118 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2419 3661 5116 618 Size: 325 217 627 632 1242 1455 1842 Fragments arranged by size: 1842 1455 1242 632 627 325 217 BanII G_rGCy'C Cuts at: 1391 2070 2150 2204 3086 3659 1391 Size: 679 80 54 882 573 4072 Fragments arranged by size: 4072 882 679 573 80 54 BbeI G_GCGC'C Cuts at: 2423 2423 Size: 6340 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2061 2877 3063 3148 3242 928 Size: 971 162 816 186 85 94 4026 Fragments arranged by size: 4026 971 816 186 162 94 85 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2217 2251 2949 2983 4531 4565 1903 Size: 34 280 34 698 34 1548 34 3678 Fragments arranged by size: 3678 1548 698 280 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4232 5442 4232 Size: 1210 5130 BclI T'GATC_A Cuts at: 1548 1548 Size: 6340 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2111 4951 136 Size: 107 122 71 631 605 439 2840 1525 Fragments arranged by size: 2840 1525 631 605 439 122 107 71 BglII A'GATC_T Cuts at: 6335 6335 Size: 6340 BlpI GC'TnA_GC Cuts at: 3757 3757 Size: 6340 Bme1580I G_kGCm'C Cuts at: 1790 2150 2204 4401 5557 1790 Size: 360 54 2197 1156 2573 Fragments arranged by size: 2573 2197 1156 360 54 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6340 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2038 3210 3294 Size: 416 53 83 399 24 621 1172 84 Cuts at: 3294 4071 5029 442 Size: 777 958 1753 Fragments arranged by size: 1753 1172 958 777 621 416 399 84 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6340 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2116 3009 2116 Size: 893 5447 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2303 2318 2564 2631 2802 5000 1239 Size: 1064 15 246 67 171 2198 2579 Fragments arranged by size: 2579 2198 1064 246 171 67 15 Bpu10I CC'TnA_GC Cuts at: 3390 3390 Size: 6340 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2475 4465 5330 5628 2475 Size: 1990 865 298 3187 Fragments arranged by size: 3187 1990 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2502 2779 5003 882 Size: 617 1003 277 2224 2219 Fragments arranged by size: 2224 2219 1003 617 277 BsaAI yAC'GTr Cuts at: 493 1204 4131 493 Size: 711 2927 2702 Fragments arranged by size: 2927 2702 711 BsaBI GATnn'nnATC Cuts at: 6334 6334 Size: 6340 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2040 2420 4147 4529 Size: 53 83 186 936 507 380 1727 382 Cuts at: 4529 5164 275 Size: 635 1451 Fragments arranged by size: 1727 1451 936 635 507 382 380 186 83 53 BsaWI w'CCGG_w Cuts at: 4773 5171 5445 5592 6003 4773 Size: 398 274 147 411 5110 Fragments arranged by size: 5110 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2680 2710 2906 2936 3436 Size: 30 165 30 1277 30 196 30 500 Cuts at: 3436 3466 1178 Size: 30 4052 Fragments arranged by size: 4052 1277 500 196 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2384 1186 Size: 1198 5142 BseYI C'CCAG_C Cuts at: 2337 2556 5543 6039 2337 Size: 219 2987 496 2638 Fragments arranged by size: 2987 2638 496 219 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5767 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3790 4551 5579 1540 Size: 275 186 1789 761 1028 2301 Fragments arranged by size: 2301 1789 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2128 3086 3396 4401 4486 5557 2128 Size: 958 310 1005 85 1071 2911 Fragments arranged by size: 2911 1071 1005 958 310 85 BsmI GAATG_Cn' Cuts at: 3851 3944 3851 Size: 93 6247 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2070 2128 2150 2204 3086 3396 3659 Size: 399 280 58 22 54 882 310 263 Cuts at: 3659 4401 4486 5557 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 882 742 399 310 280 263 85 58 54 22 BspEI T'CCGG_A Cuts at: 6003 6003 Size: 6340 BspHI T'CATG_A Cuts at: 4229 5959 4229 Size: 1730 4610 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2416 2910 3695 844 Size: 1572 494 785 3489 Fragments arranged by size: 3489 1572 785 494 BsrBI CCG'CTC Cuts at: 1394 1823 2364 4227 1394 Size: 429 541 1863 3507 Fragments arranged by size: 3507 1863 541 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2631 2874 4835 5009 65 Size: 1183 1383 243 1961 174 1396 Fragments arranged by size: 1961 1396 1383 1183 243 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2220 2416 4984 5171 1700 Size: 110 410 196 2568 187 2869 Fragments arranged by size: 2869 2568 410 196 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6340 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6340 BssSI C'ACGA_G Cuts at: 4400 5412 4400 Size: 1012 5328 BstAPI GCAn_nnn'nTGC Cuts at: 794 2455 794 Size: 1661 4679 BstBI TT'CG_AA Cuts at: 5144 5144 Size: 6340 BstEII G'GTnAC_C Cuts at: 1184 2438 1184 Size: 1254 5086 BstXI CCAn_nnnn'nTGG Cuts at: 2588 2978 2588 Size: 390 5950 BstYI r'GATC_y Cuts at: 1972 2277 2552 3670 4037 4422 4439 5891 5977 Size: 305 275 1118 367 385 17 1452 86 Cuts at: 5977 5989 6335 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1118 385 367 346 305 275 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2864 2864 Size: 6340 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4849 Fragments arranged by size: 4849 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5601 Fragments arranged by size: 5601 683 56 BtsI GCAGTG_nn' Cuts at: 3856 4650 4670 3856 Size: 794 20 5526 Fragments arranged by size: 5526 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6340 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3295 3330 529 Size: 35 2731 35 3539 Fragments arranged by size: 3539 2731 35 35 DraI TTT'AAA Cuts at: 3644 3644 Size: 6340 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5347 809 Size: 507 4031 1802 Fragments arranged by size: 4031 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3787 4676 6305 Size: 54 1425 50 275 18 1957 889 1629 Cuts at: 6305 8 Size: 43 Fragments arranged by size: 1957 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3787 1537 Size: 275 1975 4090 Fragments arranged by size: 4090 1975 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4270 1353 Size: 2917 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2092 2129 3346 4927 5301 5447 Size: 1064 138 503 37 1217 1581 374 146 Cuts at: 5447 387 Size: 1280 Fragments arranged by size: 1581 1280 1217 1064 503 374 146 138 37 EcoICRI GAG'CTC Cuts at: 3084 3084 Size: 6340 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2303 2318 2564 2631 2802 3142 3226 Size: 393 671 15 246 67 171 340 84 Cuts at: 3226 4403 5000 5787 6055 1239 Size: 1177 597 787 268 1524 Fragments arranged by size: 1524 1177 787 671 597 393 340 268 246 171 84 67 15 EcoO109I rG'GnC_Cy Cuts at: 2146 2147 2201 2652 2146 Size: 1 54 451 5834 Fragments arranged by size: 5834 451 54 1 EcoRI G'AATT_C Cuts at: 3334 3649 4027 3334 Size: 315 378 5647 Fragments arranged by size: 5647 378 315 FspI TGC'GCA Cuts at: 4846 4846 Size: 6340 HaeII r_GCGC'y Cuts at: 1678 2170 2423 5487 1678 Size: 492 253 3064 2531 Fragments arranged by size: 3064 2531 492 253 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2030 2062 2473 2505 2680 Size: 32 135 32 458 32 411 32 175 Cuts at: 2680 2712 4981 5013 5055 5087 1373 Size: 32 2269 32 42 32 2626 Fragments arranged by size: 2626 2269 458 411 175 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3684 3930 669 Size: 988 281 1746 246 3079 Fragments arranged by size: 3079 1746 988 281 246 HindIII A'AGCT_T Cuts at: 748 2828 748 Size: 2080 4260 HpaI GTT'AAC Cuts at: 3930 3930 Size: 6340 KasI G'GCGC_C Cuts at: 2419 2419 Size: 6340 KpnI G_GTAC'C Cuts at: 3665 3665 Size: 6340 MfeI C'AATT_G Cuts at: 3939 3939 Size: 6340 MluI A'CGCG_T Cuts at: 6216 6216 Size: 6340 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5454 5638 1305 Size: 4149 184 2007 Fragments arranged by size: 4149 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4518 Fragments arranged by size: 4518 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2976 3033 3117 3133 3301 3534 4298 Size: 648 1810 57 84 16 168 233 764 Cuts at: 4298 4657 4816 518 Size: 359 159 2042 Fragments arranged by size: 2042 1810 764 648 359 233 168 159 84 57 16 MspA1I CmG'CkG Cuts at: 1606 2407 2713 3756 4433 5581 5826 1606 Size: 801 306 1043 677 1148 245 2120 Fragments arranged by size: 2120 1148 1043 801 677 306 245 NaeI GCC'GGC Cuts at: 2222 2418 2222 Size: 196 6144 NarI GG'CG_CC Cuts at: 2420 2420 Size: 6340 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4849 Fragments arranged by size: 4849 939 552 NdeI CA'TA_TG Cuts at: 387 3130 387 Size: 2743 3597 NgoMIV G'CCGG_C Cuts at: 2220 2416 2220 Size: 196 6144 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6340 NotI GC'GGCC_GC Cuts at: 3787 3787 Size: 6340 NspI r_CATG'y Cuts at: 1132 1229 2897 3564 3698 5243 1132 Size: 97 1668 667 134 1545 2229 Fragments arranged by size: 2229 1668 1545 667 134 97 PasI CC'CwG_GG Cuts at: 1348 2599 2656 1348 Size: 1251 57 5032 Fragments arranged by size: 5032 1251 57 PciI A'CATG_T Cuts at: 3560 5239 3560 Size: 1679 4661 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6340 PfoI T'CCnGG_A Cuts at: 2280 2280 Size: 6340 PmeI GTTT'AAAC Cuts at: 3644 3644 Size: 6340 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4409 4441 5922 5954 4409 Size: 32 1481 32 4795 Fragments arranged by size: 4795 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2652 2652 Size: 6340 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6340 PsiI TTA'TAA Cuts at: 3465 3910 3465 Size: 445 5895 PspOMI G'GGCC_C Cuts at: 2146 2200 2146 Size: 54 6286 PstI C_TGCA'G Cuts at: 830 2053 2135 2253 2334 2615 3257 3692 830 Size: 1223 82 118 81 281 642 435 3478 Fragments arranged by size: 3478 1223 642 435 281 118 82 81 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6340 PvuII CAG'CTG Cuts at: 2407 2713 2407 Size: 306 6034 SacI G_AGCT'C Cuts at: 3086 3086 Size: 6340 SalI G'TCGA_C Cuts at: 1936 3682 1936 Size: 1746 4594 SanDI GG'GwC_CC Cuts at: 2652 2652 Size: 6340 SbfI CC_TGCA'GG Cuts at: 3692 3692 Size: 6340 ScaI AGT'ACT Cuts at: 4588 6300 4588 Size: 1712 4628 SfcI C'TryA_G Cuts at: 826 1045 2049 2131 2249 2330 2611 2737 3253 Size: 219 1004 82 118 81 281 126 516 Cuts at: 3253 3688 4823 5504 5695 826 Size: 435 1135 681 191 1471 Fragments arranged by size: 1471 1135 1004 681 516 435 281 219 191 126 118 82 81 SfoI GGC'GCC Cuts at: 2421 2421 Size: 6340 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6340 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6340 SmaI CCC'GGG Cuts at: 1872 3667 1872 Size: 1795 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2490 4444 5345 5607 820 Size: 197 926 547 1954 901 262 1553 Fragments arranged by size: 1954 1553 926 901 547 262 197 SnaBI TAC'GTA Cuts at: 493 4131 493 Size: 3638 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6340 SphI G_CATG'C Cuts at: 1132 3698 1132 Size: 2566 3774 SspI AAT'ATT Cuts at: 5 52 4264 5 Size: 47 4212 2081 Fragments arranged by size: 4212 2081 47 StuI AGG'CCT Cuts at: 1517 3006 1517 Size: 1489 4851 StyI C'CwwG_G Cuts at: 513 1065 2004 2779 3779 513 Size: 552 939 775 1000 3074 Fragments arranged by size: 3074 1000 939 775 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2329 2691 4367 4384 4537 4722 5167 Size: 1255 749 362 1676 17 153 185 445 Cuts at: 5167 325 Size: 1498 Fragments arranged by size: 1676 1498 1255 749 445 362 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4586 6298 96 Size: 274 80 33 51 1443 2609 1712 138 Fragments arranged by size: 2609 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3352 3384 3386 3418 3419 3451 6300 6332 3352 Size: 32 2 32 1 32 2849 32 3360 Fragments arranged by size: 3360 2849 32 32 32 32 2 1 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6340 XbaI T'CTAG_A Cuts at: 3676 3676 Size: 6340 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6340 XmaI C'CCGG_G Cuts at: 1870 3665 1870 Size: 1795 4545 XmnI GAAnn'nnTTC Cuts at: 3239 4469 3239 Size: 1230 5110 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4148 276 Size: 53 83 186 936 2614 2468 Fragments arranged by size: 2614 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SapI SexAI SfiI SrfI SwaI XcmI