(Circular) (Six-base) MAPSORT of: pFN21AB5545.seq Check: 5462 from: 1 to: 6577 pFN21AB5545 6577 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3113 3113 Size: 6577 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4387 278 Size: 53 83 186 936 2851 2468 Fragments arranged by size: 2851 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3541 3920 1937 Size: 1604 379 4594 Fragments arranged by size: 4594 1604 379 Acc65I G'GTAC_C Cuts at: 3898 3898 Size: 6577 AclI AA'CG_TT Cuts at: 1563 4704 5077 1563 Size: 3141 373 3063 Fragments arranged by size: 3141 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2253 2613 2889 4640 6024 6292 1632 Size: 621 360 276 1751 1384 268 1917 Fragments arranged by size: 1917 1751 1384 621 360 276 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6380 AflIII A'CryG_T Cuts at: 1203 5476 6453 1203 Size: 4273 977 1327 Fragments arranged by size: 4273 1327 977 AgeI A'CCGG_T Cuts at: 5408 5408 Size: 6577 AhdI GACnn_n'nnGTC Cuts at: 5306 5306 Size: 6577 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6577 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6577 AlwNI CAG_nnn'CTG Cuts at: 2870 4320 5892 2870 Size: 1450 1572 3555 Fragments arranged by size: 3555 1572 1450 ApaI G_GGCC'C Cuts at: 3553 3553 Size: 6577 ApaLI G'TGCA_C Cuts at: 4634 5790 4634 Size: 1156 5421 ApoI r'AATT_y Cuts at: 1493 2455 2584 2715 3153 3814 3886 4112 4264 Size: 962 129 131 438 661 72 226 152 Cuts at: 4264 6373 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 962 661 438 226 152 131 129 72 AseI AT'TA_AT Cuts at: 160 1032 5131 160 Size: 872 4099 1606 Fragments arranged by size: 4099 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6577 AvaI C'yCGr_G Cuts at: 1870 1943 3902 1870 Size: 73 1959 4545 Fragments arranged by size: 4545 1959 73 BamHI G'GATC_C Cuts at: 3907 4274 3907 Size: 367 6210 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2165 2802 3524 3898 5353 Size: 325 217 627 378 637 722 374 1455 Cuts at: 5353 618 Size: 1842 Fragments arranged by size: 1842 1455 722 637 627 378 374 325 217 BanII G_rGCy'C Cuts at: 1391 2651 3553 3896 1391 Size: 1260 902 343 4072 Fragments arranged by size: 4072 1260 902 343 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5606 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4768 4802 1903 Size: 34 2831 34 3678 Fragments arranged by size: 3678 2831 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4469 5679 4469 Size: 1210 5367 BclI T'GATC_A Cuts at: 1548 2041 1548 Size: 493 6084 BfrBI ATG'CAT Cuts at: 3701 3701 Size: 6577 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5188 136 Size: 107 122 71 631 605 3516 1525 Fragments arranged by size: 3516 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2726 6572 2726 Size: 3846 2731 BlpI GC'TnA_GC Cuts at: 3994 3994 Size: 6577 Bme1580I G_kGCm'C Cuts at: 1790 3553 4638 5794 1790 Size: 1763 1085 1156 2573 Fragments arranged by size: 2573 1763 1156 1085 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6577 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3548 4308 5266 Size: 416 53 83 399 24 2131 760 958 Cuts at: 5266 442 Size: 1753 Fragments arranged by size: 2131 1753 958 760 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6577 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3015 5237 1239 Size: 1776 2222 2579 Fragments arranged by size: 2579 2222 1776 Bpu10I CC'TnA_GC Cuts at: 3590 3590 Size: 6577 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2874 4702 5567 5865 2874 Size: 1828 865 298 3586 Fragments arranged by size: 3586 1828 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2941 3048 5240 882 Size: 617 1442 107 2192 2219 Fragments arranged by size: 2219 2192 1442 617 107 BsaAI yAC'GTr Cuts at: 493 1204 4368 493 Size: 711 3164 2702 Fragments arranged by size: 3164 2702 711 BsaBI GATnn'nnATC Cuts at: 2548 6571 2548 Size: 4023 2554 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4384 4766 5401 275 Size: 53 83 186 936 2851 382 635 1451 Fragments arranged by size: 2851 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3446 5010 5408 5682 5829 6240 3446 Size: 1564 398 274 147 411 3783 Fragments arranged by size: 3783 1564 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2490 2520 1178 Size: 30 165 30 1087 30 5235 Fragments arranged by size: 5235 1087 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2271 2274 2644 3099 3357 1186 Size: 1085 3 370 455 258 4406 Fragments arranged by size: 4406 1085 455 370 258 3 BseYI C'CCAG_C Cuts at: 5780 6276 5780 Size: 496 6081 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6004 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3799 4027 4788 5816 1540 Size: 275 186 1798 228 761 1028 2301 Fragments arranged by size: 2301 1798 1028 761 275 228 186 BsiHKAI G_wGCw'C Cuts at: 4638 4723 5794 4638 Size: 85 1071 5421 Fragments arranged by size: 5421 1071 85 BsmI GAATG_Cn' Cuts at: 4088 4181 4088 Size: 93 6484 BsmBI CGTCTCn'nnnn_ Cuts at: 2194 2771 2194 Size: 577 6000 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2651 3553 3896 4638 4723 5794 1391 Size: 399 861 902 343 742 85 1071 2174 Fragments arranged by size: 2174 1071 902 861 742 399 343 85 BspEI T'CCGG_A Cuts at: 6240 6240 Size: 6577 BspHI T'CATG_A Cuts at: 4466 6196 4466 Size: 1730 4847 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2888 3113 3932 844 Size: 2044 225 819 3489 Fragments arranged by size: 3489 2044 819 225 BsrBI CCG'CTC Cuts at: 1394 1823 3118 4464 1394 Size: 429 1295 1346 3507 Fragments arranged by size: 3507 1346 1295 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3681 5072 5246 65 Size: 1183 2433 1391 174 1396 Fragments arranged by size: 2433 1396 1391 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5221 5408 1700 Size: 110 3411 187 2869 Fragments arranged by size: 3411 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3264 96 Size: 3168 3409 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6577 BssSI C'ACGA_G Cuts at: 4637 5649 4637 Size: 1012 5565 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6577 BstBI TT'CG_AA Cuts at: 5381 5381 Size: 6577 BstEII G'GTnAC_C Cuts at: 1184 2691 1184 Size: 1507 5070 BstYI r'GATC_y Cuts at: 1972 2726 3907 4274 4659 4676 6128 6214 6226 Size: 754 1181 367 385 17 1452 86 12 Cuts at: 6226 6572 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1181 754 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5086 Fragments arranged by size: 5086 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5838 Fragments arranged by size: 5838 683 56 BtsI GCAGTG_nn' Cuts at: 2382 3787 4093 4887 4907 2382 Size: 1405 306 794 20 4052 Fragments arranged by size: 4052 1405 794 306 20 ClaI AT'CG_AT Cuts at: 1554 3795 1554 Size: 2241 4336 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2017 2052 529 Size: 35 1453 35 5054 Fragments arranged by size: 5054 1453 35 35 DraI TTT'AAA Cuts at: 3881 3881 Size: 6577 DraIII CAC_nnn'GTG Cuts at: 2809 3335 2809 Size: 526 6051 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5584 809 Size: 507 4268 1802 Fragments arranged by size: 4268 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4024 4913 6542 Size: 54 1425 50 275 18 2194 889 1629 Cuts at: 6542 8 Size: 43 Fragments arranged by size: 2194 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4024 1537 Size: 275 2212 4090 Fragments arranged by size: 4090 2212 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2184 2272 2977 3334 4507 1353 Size: 831 88 705 357 1173 3423 Fragments arranged by size: 3423 1173 831 705 357 88 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5164 5538 5684 387 Size: 1064 138 3575 374 146 1280 Fragments arranged by size: 3575 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2253 2613 2889 3015 4640 5237 6024 Size: 393 621 360 276 126 1625 597 787 Cuts at: 6024 6292 1239 Size: 268 1524 Fragments arranged by size: 1625 1524 787 621 597 393 360 276 268 126 EcoNI CCTnn'n_nnAGG Cuts at: 2619 2619 Size: 6577 EcoO109I rG'GnC_Cy Cuts at: 2608 3549 3550 2608 Size: 941 1 5635 Fragments arranged by size: 5635 941 1 EcoRI G'AATT_C Cuts at: 3153 3814 3886 4264 3153 Size: 661 72 378 5466 Fragments arranged by size: 5466 661 378 72 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2901 3594 2901 Size: 693 5884 FspI TGC'GCA Cuts at: 5083 5083 Size: 6577 HaeII r_GCGC'y Cuts at: 1678 5724 1678 Size: 4046 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2264 2296 3182 3214 3263 Size: 32 135 32 692 32 886 32 49 Cuts at: 3263 3295 5218 5250 5292 5324 1373 Size: 32 1923 32 42 32 2626 Fragments arranged by size: 2626 1923 886 692 135 49 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2434 2601 3542 3921 4167 669 Size: 988 281 496 167 941 379 246 3079 Fragments arranged by size: 3079 988 941 496 379 281 246 167 HindIII A'AGCT_T Cuts at: 748 2094 748 Size: 1346 5231 HpaI GTT'AAC Cuts at: 2434 2601 4167 2434 Size: 167 1566 4844 Fragments arranged by size: 4844 1566 167 KpnI G_GTAC'C Cuts at: 3902 3902 Size: 6577 MfeI C'AATT_G Cuts at: 4176 4176 Size: 6577 MluI A'CGCG_T Cuts at: 6453 6453 Size: 6577 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2363 2978 5691 5875 1305 Size: 1058 615 2713 184 2007 Fragments arranged by size: 2713 2007 1058 615 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4755 Fragments arranged by size: 4755 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4535 4894 5053 518 Size: 648 3369 359 159 2042 Fragments arranged by size: 3369 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2915 3993 4670 5818 6063 1606 Size: 1309 1078 677 1148 245 2120 Fragments arranged by size: 2120 1309 1148 1078 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5086 Fragments arranged by size: 5086 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6577 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6577 NotI GC'GGCC_GC Cuts at: 4024 4024 Size: 6577 NsiI A_TGCA'T Cuts at: 3703 3703 Size: 6577 NspI r_CATG'y Cuts at: 1132 1229 3935 5480 1132 Size: 97 2706 1545 2229 Fragments arranged by size: 2706 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6577 PciI A'CATG_T Cuts at: 5476 5476 Size: 6577 PflMI CCAn_nnn'nTGG Cuts at: 1108 2399 1108 Size: 1291 5286 PfoI T'CCnGG_A Cuts at: 2992 2992 Size: 6577 PmeI GTTT'AAAC Cuts at: 3881 3881 Size: 6577 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3373 3391 3405 3423 4646 4678 6159 6191 3373 Size: 18 14 18 1223 32 1481 32 3759 Fragments arranged by size: 3759 1481 1223 32 32 18 18 14 PpuMI rG'GwC_Cy Cuts at: 2608 2608 Size: 6577 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6577 PsiI TTA'TAA Cuts at: 4147 4147 Size: 6577 PspOMI G'GGCC_C Cuts at: 3549 3549 Size: 6577 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2970 3002 3355 3387 2970 Size: 32 353 32 6160 Fragments arranged by size: 6160 353 32 32 PstI C_TGCA'G Cuts at: 830 2053 3929 830 Size: 1223 1876 3478 Fragments arranged by size: 3478 1876 1223 PvuI CG_AT'CG Cuts at: 2001 3799 2001 Size: 1798 4779 PvuII CAG'CTG Cuts at: 2915 2915 Size: 6577 SalI G'TCGA_C Cuts at: 1936 3540 3919 1936 Size: 1604 379 4594 Fragments arranged by size: 4594 1604 379 SbfI CC_TGCA'GG Cuts at: 3929 3929 Size: 6577 ScaI AGT'ACT Cuts at: 2182 4825 6537 2182 Size: 2643 1712 2222 Fragments arranged by size: 2643 2222 1712 SfcI C'TryA_G Cuts at: 826 1045 2049 2207 2931 3712 3925 5060 5741 Size: 219 1004 158 724 781 213 1135 681 Cuts at: 5741 5932 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1004 781 724 681 219 213 191 158 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6577 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6577 SmaI CCC'GGG Cuts at: 1872 3904 1872 Size: 2032 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2889 4681 5582 5844 820 Size: 197 926 946 1792 901 262 1553 Fragments arranged by size: 1792 1553 946 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4368 493 Size: 3875 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6577 SphI G_CATG'C Cuts at: 1132 3935 1132 Size: 2803 3774 SspI AAT'ATT Cuts at: 5 52 4501 5 Size: 47 4449 2081 Fragments arranged by size: 4449 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6577 StyI C'CwwG_G Cuts at: 513 1065 2004 4016 513 Size: 552 939 2012 3074 Fragments arranged by size: 3074 2012 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2091 3024 4604 4621 4774 4959 5404 Size: 1255 511 933 1580 17 153 185 445 Cuts at: 5404 325 Size: 1498 Fragments arranged by size: 1580 1498 1255 933 511 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2180 3264 4823 Size: 274 80 33 51 1443 203 1084 1559 Cuts at: 4823 6535 96 Size: 1712 138 Fragments arranged by size: 1712 1559 1443 1084 274 203 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2490 2522 6537 6569 2490 Size: 32 4015 32 2498 Fragments arranged by size: 4015 2498 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6577 XbaI T'CTAG_A Cuts at: 2570 2792 3913 2570 Size: 222 1121 5234 Fragments arranged by size: 5234 1121 222 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6577 XmaI C'CCGG_G Cuts at: 1870 3902 1870 Size: 2032 4545 XmnI GAAnn'nnTTC Cuts at: 2884 4706 2884 Size: 1822 4755 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4385 276 Size: 53 83 186 936 2851 2468 Fragments arranged by size: 2851 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI