################################## # NO CUT ENZYMES ( 75 enzymes) # ################################## AclI AflII AgeI AscI AseI AsnI AvaI AviII AvrII BbvCI BcgI BciVI BfaI BfrI BlnI BsaBI BsaI BsiWI BsmI BsoBI Bsp1407I BspDI BsrDI BsrGI Bst1107I BstZ17I ClaI CpoI DraI DrdI EcoRI EcoRV EcoT22I FseI FspI HindIII HpaI KspI MaeI MamI MfeI MluI MseI MunI NheI NotI NruI NsiI PacI PaeR7I PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SfaNI SfiI SgrAI SmaI SnaBI SpeI SspBI SspI SwaI Tru9I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AatII 1783 GACGTC GACGT^C Acc65I 2366 GGTACC G^GTACC AccI 1486 GTCTAC GT^[AC][GT]AC AccIII 356 TCCGGA T^CCGGA AcsI 1014 GAATTT [GA]^AATT[CT] AhdI 1957 GACACGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 2196 GTGCAC G^TGCAC Aor51HI 374 AGCGCT AGC^GCT ApaI 2285 GGGCCC GGGCC^C ApaLI 2196 GTGCAC G^TGCAC ApoI 1014 GAATTT [GA]^AATT[CT] Asp718I 2366 GGTACC G^GTACC AspEI 1957 GACACGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 859 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 2 TGGCCA TGG^CCA BamHI 401 GGATCC G^GATCC BclI 683 TGATCA T^GATCA BglII 821 AGATCT A^GATCT BlpI 1950 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1950 GCTGAGC GC^T[ACGT]AGC BseAI 356 TCCGGA T^CCGGA BspEI 356 TCCGGA T^CCGGA BspLU11I 1043 ACATGT A^CATGT BssHII 133 GCGCGC G^CGCGC BstBI 1205 TTCGAA TT^CGAA CelII 1950 GCTGAGC GC^T[ACGT]AGC Eam1105I 1957 GACACGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 374 AGCGCT AGC^GCT FbaI 683 TGATCA T^GATCA HincII 795 GTCAAC GT[CT]^[GA]AC HindII 795 GTCAAC GT[CT]^[GA]AC KpnI 2366 GGTACC GGTAC^C MluNI 2 TGGCCA TGG^CCA MroI 356 TCCGGA T^CCGGA MscI 2 TGGCCA TGG^CCA NaeI 1574 GCCGGC GCC^GGC NdeI 172 CATATG CA^TATG NgoMIV 1574 GCCGGC G^CCGGC NspV 1205 TTCGAA TT^CGAA PflFI 859 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC SacI 2395 GAGCTC GAGCT^C SapI 1413 GAAGAGC GCTCTTC(1/4) ScaI 830 AGTACT AGT^ACT SexAI 2303 ACCTGGT A^CC[AT]GGT SfuI 1205 TTCGAA TT^CGAA SphI 1995 GCATGC GCATG^C StuI 1065 AGGCCT AGG^CCT Tsp509I 1015 AATT ^AATT Tth111I 859 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AflIII 1043 ACATGT A^C[GA][CT]GT 2174 ACGTGT AocI 1911 CCTTAGG CC^T[ACGT]AGG 2115 CCTGAGG Asp700I 957 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2124 GAAGGACTTC BbeI 60 GGCGCC GGCGC^C 2111 GGCGCC BbrPI 658 CACGTG CAC^GTG 2173 CACGTG BglI 1264 GCCGTCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2013 GCCTGCCCGGC BsaAI 658 CACGTG [CT]AC^GT[GA] 2173 CACGTG BsaWI 356 TCCGGA [AT]^CCGG[AT] 694 TCCGGT BsgI 1840 CTGCAC GTGCAG(16/14) 1361 GTGCAG BsiEI 33 CGGCCG CG[GA][CT]^CG 881 CGGCCG BspMI 1906 ACCTGC ACCTGC(4/8) 1236 GCAGGT BstAPI 871 GCAGGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1824 GCAGAGTGTGC BstEII 1857 GGTCACC G^GT[ACGT]ACC 2343 GGTGACC BstPI 1857 GGTCACC G^GT[ACGT]ACC 2343 GGTGACC Bsu36I 1911 CCTTAGG CC^T[ACGT]AGG 2115 CCTGAGG EagI 33 CGGCCG C^GGCCG 881 CGGCCG EarI 1429 CTCTTC CTCTTC(1/4) 1413 GAAGAG EclXI 33 CGGCCG C^GGCCG 881 CGGCCG Eco52I 33 CGGCCG C^GGCCG 881 CGGCCG Eco81I 1911 CCTTAGG CC^T[ACGT]AGG 2115 CCTGAGG EcoNI 321 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1907 CCTGCCTTAGG EcoO65I 1857 GGTCACC G^GT[ACGT]ACC 2343 GGTGACC KasI 60 GGCGCC G^GCGCC 2111 GGCGCC Ksp632I 1429 CTCTTC CTCTTC(1/4) 1413 GAAGAG NarI 60 GGCGCC GG^CGCC 2111 GGCGCC PleI 1090 GACTC GAGTC(4/5) 164 GAGTC PmaCI 658 CACGTG CAC^GTG 2173 CACGTG PmlI 658 CACGTG CAC^GTG 2173 CACGTG SbfI 1233 CCTGCAGG CCTGCA^GG 1320 CCTGCAGG SfoI 60 GGCGCC GGC^GCC 2111 GGCGCC Sse8387I 1233 CCTGCAGG CCTGCA^GG 1320 CCTGCAGG TfiI 614 GAATC G^A[AT]TC 1208 GAATC XmnI 957 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2124 GAAGGACTTC ######################################### # 3 OR MORE CUT ENZYMES ( 101 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AspHI AvaII BanI BanII BbsI BbvI BcnI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI Bsp1286I BspHI BsrBI BsrFI BsrI BssKI BssSI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PpuMI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfcI SmlI StyI TaqI TseI Tsp45I TspRI TthHB8I XcmI XhoII