(Circular) (Six-base) MAPSORT of: pFN21AE4581.seq Check: 2074 from: 1 to: 7120 pFN21AE4581 7120 bp With 182 enzymes: SgfI * July 6, 2012 17:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3232 3232 Size: 7120 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3792 4930 278 Size: 53 83 186 936 2256 1138 2468 Fragments arranged by size: 2468 2256 1138 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3492 4463 1937 Size: 1555 971 4594 Fragments arranged by size: 4594 1555 971 Acc65I G'GTAC_C Cuts at: 4371 4441 4371 Size: 70 7050 AclI AA'CG_TT Cuts at: 1563 5247 5620 1563 Size: 3684 373 3063 Fragments arranged by size: 3684 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4148 5183 6567 6835 1632 Size: 2516 1035 1384 268 1917 Fragments arranged by size: 2516 1917 1384 1035 268 AfeI AGC'GCT Cuts at: 2381 2381 Size: 7120 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6923 AflIII A'CryG_T Cuts at: 1203 3048 4179 6019 6996 1203 Size: 1845 1131 1840 977 1327 Fragments arranged by size: 1845 1840 1327 1131 977 AgeI A'CCGG_T Cuts at: 5951 5951 Size: 7120 AhdI GACnn_n'nnGTC Cuts at: 3967 5849 3967 Size: 1882 5238 AleI CACnn'nnGTG Cuts at: 1166 3905 1166 Size: 2739 4381 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7120 AlwNI CAG_nnn'CTG Cuts at: 2882 2971 3325 4036 4863 6435 2882 Size: 89 354 711 827 1572 3567 Fragments arranged by size: 3567 1572 827 711 354 89 ApaI G_GGCC'C Cuts at: 4294 4294 Size: 7120 ApaLI G'TGCA_C Cuts at: 4201 5177 6333 4201 Size: 976 1156 4988 Fragments arranged by size: 4988 1156 976 ApoI r'AATT_y Cuts at: 1493 3019 4429 4655 4807 6916 1493 Size: 1526 1410 226 152 2109 1697 Fragments arranged by size: 2109 1697 1526 1410 226 152 AseI AT'TA_AT Cuts at: 160 1032 5674 160 Size: 872 4642 1606 Fragments arranged by size: 4642 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7120 AvaI C'yCGr_G Cuts at: 1870 1943 4445 1870 Size: 73 2502 4545 Fragments arranged by size: 4545 2502 73 BamHI G'GATC_C Cuts at: 2406 4450 4817 2406 Size: 2044 367 4709 Fragments arranged by size: 4709 2044 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2065 4116 4246 4305 4371 Size: 325 217 627 278 2051 130 59 66 Cuts at: 4371 4441 5896 618 Size: 70 1455 1842 Fragments arranged by size: 2051 1842 1455 627 325 278 217 130 70 66 59 BanII G_rGCy'C Cuts at: 1391 2576 2914 3338 3525 4294 4404 4439 1391 Size: 1185 338 424 187 769 110 35 4072 Fragments arranged by size: 4072 1185 769 424 338 187 110 35 BbeI G_GCGC'C Cuts at: 2069 4120 2069 Size: 2051 5069 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3127 3535 3784 928 Size: 971 1228 408 249 4264 Fragments arranged by size: 4264 1228 971 408 249 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5311 5345 1903 Size: 34 3374 34 3678 Fragments arranged by size: 3678 3374 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5012 6222 5012 Size: 1210 5910 BclI T'GATC_A Cuts at: 1548 2688 1548 Size: 1140 5980 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3275 4024 5731 Size: 107 122 71 631 605 1603 749 1707 Cuts at: 5731 136 Size: 1525 Fragments arranged by size: 1707 1603 1525 749 631 605 122 107 71 BglII A'GATC_T Cuts at: 2826 7115 2826 Size: 4289 2831 BlpI GC'TnA_GC Cuts at: 3956 4537 3956 Size: 581 6539 Bme1580I G_kGCm'C Cuts at: 1790 3840 4205 4251 4294 5181 6337 1790 Size: 2050 365 46 43 887 1156 2573 Fragments arranged by size: 2573 2050 1156 887 365 46 43 BmgBI CAC'GTC Cuts at: 1318 3952 1318 Size: 2634 4486 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3859 4851 5809 Size: 416 53 83 399 24 2442 992 958 Cuts at: 5809 442 Size: 1753 Fragments arranged by size: 2442 1753 992 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7120 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3400 4420 3400 Size: 1020 6100 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2348 2998 4013 5780 1239 Size: 1109 650 1015 1767 2579 Fragments arranged by size: 2579 1767 1109 1015 650 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2264 2450 2660 5245 6110 6408 2264 Size: 186 210 2585 865 298 2976 Fragments arranged by size: 2976 2585 865 298 210 186 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5783 882 Size: 617 4284 2219 Fragments arranged by size: 4284 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2665 4180 4911 493 Size: 711 1461 1515 731 2702 Fragments arranged by size: 2702 1515 1461 731 711 BsaBI GATnn'nnATC Cuts at: 7114 7114 Size: 7120 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2066 2105 3789 3939 Size: 53 83 186 936 533 39 1684 150 Cuts at: 3939 3968 4117 4143 4259 4927 5309 5944 275 Size: 29 149 26 116 668 382 635 1451 Fragments arranged by size: 1684 1451 936 668 635 533 382 186 150 149 116 83 53 39 29 26 BsaWI w'CCGG_w Cuts at: 2361 2699 5553 5951 6225 6372 6783 2361 Size: 338 2854 398 274 147 411 2698 Fragments arranged by size: 2854 2698 411 398 338 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2855 2885 2901 2931 2936 Size: 30 165 30 1452 30 16 30 5 Cuts at: 2936 2966 1178 Size: 30 5332 Fragments arranged by size: 5332 1452 165 30 30 30 30 30 16 5 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2072 2345 2348 2957 4412 1186 Size: 886 273 3 609 1455 3894 Fragments arranged by size: 3894 1455 886 609 273 3 BseYI C'CCAG_C Cuts at: 2123 2382 3666 3670 4014 4050 6323 6819 2123 Size: 259 1284 4 344 36 2273 496 2424 Fragments arranged by size: 2424 2273 1284 496 344 259 36 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3387 3830 1163 Size: 573 1651 443 4453 Fragments arranged by size: 4453 1651 573 443 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2041 2889 4570 5331 6359 1540 Size: 275 186 40 848 1681 761 1028 2301 Fragments arranged by size: 2301 1681 1028 848 761 275 186 40 BsiHKAI G_wGCw'C Cuts at: 2568 3765 3902 4205 4404 5181 5266 6337 2568 Size: 1197 137 303 199 777 85 1071 3351 Fragments arranged by size: 3351 1197 1071 777 303 199 137 85 BsmI GAATG_Cn' Cuts at: 4631 4724 4631 Size: 93 7027 BsmBI CGTCTCn'nnnn_ Cuts at: 2020 2875 4134 2020 Size: 855 1259 5006 Fragments arranged by size: 5006 1259 855 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2568 2576 2914 3338 3525 3765 3840 Size: 399 778 8 338 424 187 240 75 Cuts at: 3840 3902 4205 4251 4294 4404 4439 5181 5266 Size: 62 303 46 43 110 35 742 85 Cuts at: 5266 6337 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 778 742 424 399 338 303 240 187 110 85 75 62 46 43 35 8 BspEI T'CCGG_A Cuts at: 2361 6783 2361 Size: 4422 2698 BspHI T'CATG_A Cuts at: 3711 3765 4224 5009 6739 3711 Size: 54 459 785 1730 4092 Fragments arranged by size: 4092 1730 785 459 54 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3232 3920 4475 844 Size: 2388 688 555 3489 Fragments arranged by size: 3489 2388 688 555 BsrBI CCG'CTC Cuts at: 1394 1823 2338 4090 4195 5007 1394 Size: 429 515 1752 105 812 3507 Fragments arranged by size: 3507 1752 812 515 429 105 BsrDI GCAATG_nn' Cuts at: 65 1248 5615 5789 65 Size: 1183 4367 174 1396 Fragments arranged by size: 4367 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2599 3228 3579 5764 5951 1700 Size: 110 789 629 351 2185 187 2869 Fragments arranged by size: 2869 2185 789 629 351 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7120 BssHII G'CGCG_C Cuts at: 1925 2138 1925 Size: 213 6907 BssSI C'ACGA_G Cuts at: 2420 2567 3122 5180 6192 2420 Size: 147 555 2058 1012 3348 Fragments arranged by size: 3348 2058 1012 555 147 BstAPI GCAn_nnn'nTGC Cuts at: 794 2882 3835 794 Size: 2088 953 4079 Fragments arranged by size: 4079 2088 953 BstBI TT'CG_AA Cuts at: 3211 5924 3211 Size: 2713 4407 BstEII G'GTnAC_C Cuts at: 1184 3862 4348 1184 Size: 2678 486 3956 Fragments arranged by size: 3956 2678 486 BstXI CCAn_nnnn'nTGG Cuts at: 3151 3757 3781 3151 Size: 606 24 6490 Fragments arranged by size: 6490 606 24 BstYI r'GATC_y Cuts at: 1972 2250 2406 2655 2784 2826 2982 3126 3591 Size: 278 156 249 129 42 156 144 465 Cuts at: 3591 4450 4817 5202 5219 6671 6757 6769 7115 Size: 859 367 385 17 1452 86 12 346 Cuts at: 7115 1972 Size: 1977 Fragments arranged by size: 1977 1452 859 465 385 367 346 278 249 156 156 144 129 86 42 17 12 Bsu36I CC'TnA_GG Cuts at: 3917 4121 3917 Size: 204 6916 BtgI C'CryG_G Cuts at: 513 1065 2004 2715 2733 2916 3040 3144 3387 Size: 552 939 711 18 183 124 104 243 Cuts at: 3387 3990 4206 4268 513 Size: 603 216 62 3365 Fragments arranged by size: 3365 939 711 603 552 243 216 183 124 104 62 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3124 487 Size: 683 56 1898 4483 Fragments arranged by size: 4483 1898 683 56 BtsI GCAGTG_nn' Cuts at: 3180 3879 4636 5430 5450 3180 Size: 699 757 794 20 4850 Fragments arranged by size: 4850 794 757 699 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7120 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7085 DraI TTT'AAA Cuts at: 4424 4424 Size: 7120 DraIII CAC_nnn'GTG Cuts at: 2668 4078 4312 2668 Size: 1410 234 5476 Fragments arranged by size: 5476 1410 234 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6127 809 Size: 507 4811 1802 Fragments arranged by size: 4811 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2038 2730 Size: 54 1425 50 275 18 177 31 692 Cuts at: 2730 2886 3264 3276 4567 5456 7085 8 Size: 156 378 12 1291 889 1629 43 Fragments arranged by size: 1629 1425 1291 889 692 378 275 177 156 54 50 43 31 18 12 EagI C'GGCC_G Cuts at: 1537 1812 2038 2886 4567 1537 Size: 275 226 848 1681 4090 Fragments arranged by size: 4090 1681 848 275 226 EarI CTCTTCn'nnn_ Cuts at: 1353 3413 3440 5050 1353 Size: 2060 27 1610 3423 Fragments arranged by size: 3423 2060 1610 27 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2356 2529 3644 5707 6081 6227 Size: 1064 138 767 173 1115 2063 374 146 Cuts at: 6227 387 Size: 1280 Fragments arranged by size: 2063 1280 1115 1064 767 374 173 146 138 EcoICRI GAG'CTC Cuts at: 4402 4402 Size: 7120 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2348 2998 4013 4148 5183 5780 6567 Size: 393 716 650 1015 135 1035 597 787 Cuts at: 6567 6835 1239 Size: 268 1524 Fragments arranged by size: 1524 1035 1015 787 716 650 597 393 268 135 EcoNI CCTnn'n_nnAGG Cuts at: 2330 3916 2330 Size: 1586 5534 EcoO109I rG'GnC_Cy Cuts at: 2725 2926 3055 3802 3856 4033 4291 4360 2725 Size: 201 129 747 54 177 258 69 5485 Fragments arranged by size: 5485 747 258 201 177 129 69 54 EcoRI G'AATT_C Cuts at: 4429 4807 4429 Size: 378 6742 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2881 4408 2881 Size: 1527 5593 FspI TGC'GCA Cuts at: 5626 5626 Size: 7120 HaeII r_GCGC'y Cuts at: 1678 2069 2383 4120 6267 1678 Size: 391 314 1737 2147 2531 Fragments arranged by size: 2531 2147 1737 391 314 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2855 2887 2936 2968 3953 Size: 32 135 32 1283 32 49 32 985 Cuts at: 3953 3985 5761 5793 5835 5867 1373 Size: 32 1776 32 42 32 2626 Fragments arranged by size: 2626 1776 1283 985 135 49 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2802 4464 4710 669 Size: 988 281 864 1662 246 3079 Fragments arranged by size: 3079 1662 988 864 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7120 HpaI GTT'AAC Cuts at: 4710 4710 Size: 7120 KasI G'GCGC_C Cuts at: 2065 4116 2065 Size: 2051 5069 KpnI G_GTAC'C Cuts at: 4375 4445 4375 Size: 70 7050 MfeI C'AATT_G Cuts at: 4719 4719 Size: 7120 MluI A'CGCG_T Cuts at: 6996 6996 Size: 7120 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2152 4119 6234 6418 1305 Size: 847 1967 2115 184 2007 Fragments arranged by size: 2115 2007 1967 847 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 10 Size: 54 1425 343 177 5121 Fragments arranged by size: 5121 1425 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2444 3143 3149 3713 3758 3905 4005 Size: 648 1278 699 6 564 45 147 100 Cuts at: 4005 4178 5078 5437 5596 518 Size: 173 900 359 159 2042 Fragments arranged by size: 2042 1278 900 699 648 564 359 173 159 147 100 45 6 MspA1I CmG'CkG Cuts at: 1606 2071 2110 2320 2616 2900 3352 3670 3826 Size: 465 39 210 296 284 452 318 156 Cuts at: 3826 4114 4536 5213 6361 6606 1606 Size: 288 422 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 465 452 422 318 296 288 284 245 210 156 39 NaeI GCC'GGC Cuts at: 3581 3581 Size: 7120 NarI GG'CG_CC Cuts at: 2066 4117 2066 Size: 2051 5069 NcoI C'CATG_G Cuts at: 513 1065 2004 3144 3387 4206 513 Size: 552 939 1140 243 819 3427 Fragments arranged by size: 3427 1140 939 819 552 243 NdeI CA'TA_TG Cuts at: 387 2178 387 Size: 1791 5329 NgoMIV G'CCGG_C Cuts at: 3579 3579 Size: 7120 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7120 NotI GC'GGCC_GC Cuts at: 4567 4567 Size: 7120 NspI r_CATG'y Cuts at: 1132 1229 3052 3817 4004 4478 6023 1132 Size: 97 1823 765 187 474 1545 2229 Fragments arranged by size: 2229 1823 1545 765 474 187 97 PasI CC'CwG_GG Cuts at: 1348 4294 1348 Size: 2946 4174 PciI A'CATG_T Cuts at: 3048 6019 3048 Size: 2971 4149 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7120 PfoI T'CCnGG_A Cuts at: 2325 2325 Size: 7120 PmeI GTTT'AAAC Cuts at: 4424 4424 Size: 7120 PmlI CAC'GTG Cuts at: 2665 4180 2665 Size: 1515 5605 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5189 5221 6702 6734 5189 Size: 32 1481 32 5575 Fragments arranged by size: 5575 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2926 3055 4360 2926 Size: 129 1305 5686 Fragments arranged by size: 5686 1305 129 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7120 PsiI TTA'TAA Cuts at: 4690 4690 Size: 7120 PspOMI G'GGCC_C Cuts at: 4290 4290 Size: 7120 PstI C_TGCA'G Cuts at: 830 3243 3330 3357 3831 4472 830 Size: 2413 87 27 474 641 3478 Fragments arranged by size: 3478 2413 641 474 87 27 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7120 PvuII CAG'CTG Cuts at: 2320 3670 3826 2320 Size: 1350 156 5614 Fragments arranged by size: 5614 1350 156 SacI G_AGCT'C Cuts at: 4404 4404 Size: 7120 SalI G'TCGA_C Cuts at: 1936 4462 1936 Size: 2526 4594 SapI GCTCTTCn'nnn_ Cuts at: 3413 3413 Size: 7120 SbfI CC_TGCA'GG Cuts at: 3243 3330 4472 3243 Size: 87 1142 5891 Fragments arranged by size: 5891 1142 87 ScaI AGT'ACT Cuts at: 2837 5368 7080 2837 Size: 2531 1712 2877 Fragments arranged by size: 2877 2531 1712 SexAI A'CCwGG_T Cuts at: 4308 4308 Size: 7120 SfcI C'TryA_G Cuts at: 826 1045 3239 3326 3353 3827 4468 5603 6284 Size: 219 2194 87 27 474 641 1135 681 Cuts at: 6284 6475 826 Size: 191 1471 Fragments arranged by size: 2194 1471 1135 681 641 474 219 191 87 27 SfoI GGC'GCC Cuts at: 2067 4118 2067 Size: 2051 5069 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7120 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7120 SmaI CCC'GGG Cuts at: 1872 4447 1872 Size: 2575 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2279 2465 2675 5224 6125 6387 Size: 197 926 336 186 210 2549 901 262 Cuts at: 6387 820 Size: 1553 Fragments arranged by size: 2549 1553 926 901 336 262 210 197 186 SnaBI TAC'GTA Cuts at: 493 4911 493 Size: 4418 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7120 SphI G_CATG'C Cuts at: 1132 4004 4478 1132 Size: 2872 474 3774 Fragments arranged by size: 3774 2872 474 SspI AAT'ATT Cuts at: 5 52 5044 5 Size: 47 4992 2081 Fragments arranged by size: 4992 2081 47 StuI AGG'CCT Cuts at: 1517 3072 1517 Size: 1555 5565 StyI C'CwwG_G Cuts at: 513 1065 2004 2244 3144 3387 4206 4559 513 Size: 552 939 240 900 243 819 353 3074 Fragments arranged by size: 3074 939 900 819 552 353 243 240 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2909 4028 4177 5147 5164 5317 5502 Size: 1255 1329 1119 149 970 17 153 185 Cuts at: 5502 5947 325 Size: 445 1498 Fragments arranged by size: 1498 1329 1255 1119 970 445 185 153 149 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2469 2835 3378 Size: 274 80 33 51 1443 492 366 543 Cuts at: 3378 5366 7078 96 Size: 1988 1712 138 Fragments arranged by size: 1988 1712 1443 543 492 366 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2223 2255 2901 2933 7080 7112 2223 Size: 32 646 32 4147 32 2231 Fragments arranged by size: 4147 2231 646 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2867 1316 Size: 1551 5569 XbaI T'CTAG_A Cuts at: 4456 4456 Size: 7120 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3151 4102 4375 3151 Size: 951 273 5896 Fragments arranged by size: 5896 951 273 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7120 XmaI C'CCGG_G Cuts at: 1870 4445 1870 Size: 2575 4545 XmnI GAAnn'nnTTC Cuts at: 2966 4133 5249 2966 Size: 1167 1116 4837 Fragments arranged by size: 4837 1167 1116 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3790 4928 276 Size: 53 83 186 936 2256 1138 2468 Fragments arranged by size: 2468 2256 1138 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstZ17I EcoRV FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SacII SanDI SfiI SrfI SwaI