################################## # NO CUT ENZYMES ( 197 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsmFI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssKI BssSI Bst1107I BstBI BstEII BstNI BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DdeI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HaeIII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HphI KasI KpnI KspI MaeII MamI MflI MluI MluNI MroI MscI MspA1I MvaI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI Sau96I SbfI ScaI ScrFI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI TspRI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AciI 431 CCGC CCGC(-3/-1) AfaI 311 GTAC GT^AC BanI 240 GGCACC G^G[CT][GA]CC BanII 427 GAGCCC G[GA]GC[CT]^C BbvI 447 GCTGC GCAGC(8/12) BmyI 427 GAGCCC G[AGT]GC[ACT]^C BpmI 118 CTGGAG CTGGAG(16/14) BsgI 170 GTGCAG GTGCAG(16/14) BsmI 480 GAATGC GAATGC(1/-1) Bsp1286I 427 GAGCCC G[AGT]GC[ACT]^C BspHI 221 TCATGA T^CATGA BsrDI 347 GCAATG GCAATG(2/0) BsrI 82 CCAGT ACTGG(1/-1) BstAPI 136 GCAAAACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC EarI 207 GAAGAG CTCTTC(1/4) FauI 430 CCCGC CCCGC(4/6) Fnu4HI 447 GCTGC GC^[ACGT]GC HapII 12 CCGG C^CGG HpaII 12 CCGG C^CGG ItaI 447 GCTGC GC^[ACGT]GC Ksp632I 207 GAAGAG CTCTTC(1/4) MaeIII 31 GTGAC ^GT[ACGT]AC MfeI 359 CAATTG C^AATTG MslI 227 CACTAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 12 CCGG C^CGG MunI 359 CAATTG C^AATTG NheI 132 GCTAGC G^CTAGC NlaIV 240 GGCACC GG[ACGT]^[ACGT]CC NspI 333 GCATGT [GA]CATG^[CT] PleI 352 GAGTC GAGTC(4/5) PstI 448 CTGCAG CTGCA^G RcaI 221 TCATGA T^CATGA RsaI 311 GTAC GT^AC SfaNI 436 GATGC GCATC(5/9) SspI 43 AATATT AAT^ATT TaqI 17 TCGA T^CGA TseI 447 GCTGC G^C[AT]GC Tsp45I 31 GTGAC ^GT[GC]AC Tsp509I 360 AATT ^AATT TthHB8I 17 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 7 enzymes) # ################################### AlwI 14 GGATC GGATC(4/5) 189 GGATC BsmAI 210 GAGAC GTCTC(1/5) 397 GAGAC BstF5I 111 GGATG GGATG(2/0) 232 GGATG FokI 111 GGATG GGATG(9/13) 232 GGATG MboII 207 GAAGA GAAGA(8/7) 405 GAAGA MwoI 136 GCAAAACTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 163 GCTGTAGGTGC TfiI 66 GAATC G^A[AT]TC 201 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 14 enzymes) # ######################################### AluI BfaI Cac8I DpnII HinfI MaeI MboI MnlI MseI NdeII NlaIII Sau3AI SfcI Tru9I