################################## # NO CUT ENZYMES ( 138 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BbrPI BbsI BbvCI BcgI BcnI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT14I EcoT22I FauI FseI FspI HapII HgaI HincII HindII HindIII HpaI HpaII KpnI KspI MaeIII MamI MfeI MluI MroI MspI MunI MvnI NaeI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StyI SwaI Tsp45I Tth111I Van91I XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AcsI 319 AAATTT [GA]^AATT[CT] AcyI 1054 GGCGCC G[GA]^CG[CT]C ApoI 319 AAATTT [GA]^AATT[CT] AvaI 97 CTCGAG C^[CT]CG[GA]G BalI 1073 TGGCCA TGG^CCA BamHI 968 GGATCC G^GATCC BanI 1054 GGCGCC G^G[CT][GA]CC BanII 408 GAGCCC G[GA]GC[CT]^C BbeI 1054 GGCGCC GGCGC^C BciVI 928 GGATAC GTATCC(6/5) BclI 425 TGATCA T^GATCA BfaI 778 CTAG C^TAG BsaAI 726 CACGTA [CT]AC^GT[GA] BsaHI 1054 GGCGCC G[GA]^CG[CT]C BsaI 915 GGTCTC GGTCTC(1/5) BseRI 836 GAGGAG GAGGAG(10/8) BsmI 819 GAATGC GAATGC(1/-1) BsoBI 97 CTCGAG C^[CT]CG[GA]G BspDI 569 ATCGAT AT^CGAT BstF5I 656 GGATG GGATG(2/0) BstYI 968 GGATCC [GA]^GATC[CT] CfoI 1055 GCGC GCG^C ClaI 569 ATCGAT AT^CGAT DsaI 999 CCACGG C^C[GA][CT]GG EarI 601 GAAGAG CTCTTC(1/4) EcoRV 378 GATATC GAT^ATC FbaI 425 TGATCA T^GATCA FokI 656 GGATG GGATG(9/13) HaeII 1054 GGCGCC [GA]GCGC^[CT] HhaI 1055 GCGC GCG^C Hin1I 1054 GGCGCC G[GA]^CG[CT]C HinP1I 1055 GCGC G^CGC KasI 1054 GGCGCC G^GCGCC Ksp632I 601 GAAGAG CTCTTC(1/4) MaeI 778 CTAG C^TAG MflI 968 GGATCC [GA]^GATC[CT] MluNI 1073 TGGCCA TGG^CCA MscI 1073 TGGCCA TGG^CCA MspA1I 1085 CAGCTG C[AC]G^C[GT]G NarI 1054 GGCGCC GG^CGCC PaeR7I 97 CTCGAG C^TCGAG PpuMI 1211 AGGTCCC [GA]G^G[AT]CC[CT] PvuII 1085 CAGCTG CAG^CTG SfoI 1054 GGCGCC GGC^GCC SmlI 97 CTCGAG C^T[CT][GA]AG SspI 483 AATATT AAT^ATT XhoI 97 CTCGAG C^TCGAG XhoII 968 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AciI 608 CCGC CCGC(-3/-1) 859 GCGG AspHI 751 GTGCTC G[AT]GC[AT]^C 784 GAGCAC BsiHKAI 751 GTGCTC G[AT]GC[AT]^C 784 GAGCAC BsmAI 698 GAGAC GTCTC(1/5) 916 GTCTC Bsp1407I 168 TGTACA T^GTACA 288 TGTACA BspHI 203 TCATGA T^CATGA 428 TCATGA BspLU11I 171 ACATGT A^CATGT 979 ACATGT BsrGI 168 TGTACA T^GTACA 288 TGTACA BsrI 1092 ACTGG ACTGG(1/-1) 273 CCAGT BstXI 522 CCATGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 773 CCAGCCTAGTGG DraII 532 GGGGCCT [GA]G^G[ACGT]CC[CT] 1211 AGGTCCC EaeI 680 CGGCCA [CT]^GGCC[GA] 1073 TGGCCA EcoO109I 532 GGGGCCT [GA]G^G[ACGT]CC[CT] 1211 AGGTCCC HphI 113 TCACC GGTGA(8/7) 873 TCACC MaeII 727 ACGT A^CGT 1066 ACGT MboII 216 GAAGA GAAGA(8/7) 601 GAAGA MslI 414 CACCCAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1065 CACGTCTATG NspI 171 ACATGT [GA]CATG^[CT] 979 ACATGT RcaI 203 TCATGA T^CATGA 428 TCATGA SexAI 470 ACCTGGT A^CC[AT]GGT 1008 ACCTGGT SfaNI 508 GCATC GCATC(5/9) 560 GCATC SfcI 26 CTATAG C^T[GA][CT]AG 551 CTGTAG SspBI 168 TGTACA T^GTACA 288 TGTACA StuI 491 AGGCCT AGG^CCT 1023 AGGCCT TaqI 98 TCGA T^CGA 570 TCGA TfiI 332 GAATC G^A[AT]TC 1195 GATTC TthHB8I 98 TCGA T^CGA 570 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 45 enzymes) # ######################################### AfaI AflIII AluI AlwI AvaII BbvI BglI BmyI BpmI BsaJI BsgI BsiYI BslI BsmFI Bsp1286I BsrDI BssKI BstNI Cac8I Cfr13I DdeI DpnII EcoRII Fnu4HI HaeIII HinfI ItaI MboI MnlI MseI MvaI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI Tru9I TseI Tsp509I TspRI XcmI