(Circular) (Six-base) MAPSORT of: pFN21AE1418.seq Check: 7014 from: 1 to: 5932 pFN21AE1418 5932 bp With 182 enzymes: SgfI * August 16, 2010 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3742 278 Size: 53 83 186 936 2206 2468 Fragments arranged by size: 2468 2206 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3275 1937 Size: 1338 4594 Acc65I G'GTAC_C Cuts at: 3253 3253 Size: 5932 AclI AA'CG_TT Cuts at: 1563 4059 4432 1563 Size: 2496 373 3063 Fragments arranged by size: 3063 2496 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2218 2240 2347 3995 5379 5647 1632 Size: 586 22 107 1648 1384 268 1917 Fragments arranged by size: 1917 1648 1384 586 268 107 22 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5735 AflIII A'CryG_T Cuts at: 1203 2176 2730 2984 3069 4831 5808 1203 Size: 973 554 254 85 1762 977 1327 Fragments arranged by size: 1762 1327 977 973 554 254 85 AgeI A'CCGG_T Cuts at: 4763 4763 Size: 5932 AhdI GACnn_n'nnGTC Cuts at: 4661 4661 Size: 5932 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5932 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3108 1184 Size: 1924 4008 AlwNI CAG_nnn'CTG Cuts at: 3675 5247 3675 Size: 1572 4360 ApaLI G'TGCA_C Cuts at: 3989 5145 3989 Size: 1156 4776 ApoI r'AATT_y Cuts at: 1493 2324 3241 3467 3619 5728 1493 Size: 831 917 226 152 2109 1697 Fragments arranged by size: 2109 1697 917 831 226 152 AseI AT'TA_AT Cuts at: 160 1032 4486 160 Size: 872 3454 1606 Fragments arranged by size: 3454 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5932 AvaI C'yCGr_G Cuts at: 1870 1943 2102 3257 1870 Size: 73 159 1155 4545 Fragments arranged by size: 4545 1155 159 73 BamHI G'GATC_C Cuts at: 2973 3262 3629 2973 Size: 289 367 5276 Fragments arranged by size: 5276 367 289 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3059 3253 4708 618 Size: 325 217 627 1272 194 1455 1842 Fragments arranged by size: 1842 1455 1272 627 325 217 194 BanII G_rGCy'C Cuts at: 1391 2417 3251 1391 Size: 1026 834 4072 Fragments arranged by size: 4072 1026 834 BbeI G_GCGC'C Cuts at: 3063 3063 Size: 5932 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4961 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4123 4157 1903 Size: 34 2186 34 3678 Fragments arranged by size: 3678 2186 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2927 3824 5034 2927 Size: 897 1210 3825 Fragments arranged by size: 3825 1210 897 BclI T'GATC_A Cuts at: 1548 2430 1548 Size: 882 5050 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2537 3036 3086 Size: 107 122 71 631 605 865 499 50 Cuts at: 3086 4543 136 Size: 1457 1525 Fragments arranged by size: 1525 1457 865 631 605 499 122 107 71 50 BglII A'GATC_T Cuts at: 5927 5927 Size: 5932 BlpI GC'TnA_GC Cuts at: 3349 3349 Size: 5932 Bme1580I G_kGCm'C Cuts at: 1790 2288 2858 3993 5149 1790 Size: 498 570 1135 1156 2573 Fragments arranged by size: 2573 1156 1135 570 498 BmgBI CAC'GTC Cuts at: 1318 3072 1318 Size: 1754 4178 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3107 3663 4621 Size: 416 53 83 399 24 1690 556 958 Cuts at: 4621 442 Size: 1753 Fragments arranged by size: 1753 1690 958 556 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5932 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2819 2819 Size: 5932 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2261 2721 2761 2859 4592 1239 Size: 1022 460 40 98 1733 2579 Fragments arranged by size: 2579 1733 1022 460 98 40 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4057 4922 5220 4057 Size: 865 298 4769 Fragments arranged by size: 4769 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2926 4595 882 Size: 617 1427 1669 2219 Fragments arranged by size: 2219 1669 1427 617 BsaAI yAC'GTr Cuts at: 493 1204 2733 3723 493 Size: 711 1529 990 2702 Fragments arranged by size: 2702 1529 990 711 BsaBI GATnn'nnATC Cuts at: 5926 5926 Size: 5932 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3060 3739 4121 4756 Size: 53 83 186 936 1527 679 382 635 Cuts at: 4756 275 Size: 1451 Fragments arranged by size: 1527 1451 936 679 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4365 4763 5037 5184 5595 4365 Size: 398 274 147 411 4702 Fragments arranged by size: 4702 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2835 2865 1178 Size: 30 165 30 1432 30 4245 Fragments arranged by size: 4245 1432 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2856 1186 Size: 1670 4262 BseYI C'CCAG_C Cuts at: 2672 5135 5631 2672 Size: 2463 496 2973 Fragments arranged by size: 2973 2463 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2388 2734 3078 1163 Size: 573 652 346 344 4017 Fragments arranged by size: 4017 652 573 346 344 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3382 4143 5171 1540 Size: 275 186 1381 761 1028 2301 Fragments arranged by size: 2301 1381 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2760 2793 3993 4078 5149 2760 Size: 33 1200 85 1071 3543 Fragments arranged by size: 3543 1200 1071 85 33 BsmI GAATG_Cn' Cuts at: 2830 3443 3536 2830 Size: 613 93 5226 Fragments arranged by size: 5226 613 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2288 2417 2760 2793 2858 3251 3993 Size: 399 498 129 343 33 65 393 742 Cuts at: 3993 4078 5149 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 498 399 393 343 129 85 65 33 BspEI T'CCGG_A Cuts at: 5595 5595 Size: 5932 BspHI T'CATG_A Cuts at: 2208 2433 3821 5551 2208 Size: 225 1388 1730 2589 Fragments arranged by size: 2589 1730 1388 225 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3287 844 Size: 2443 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3819 1394 Size: 429 1996 3507 Fragments arranged by size: 3507 1996 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2643 2688 2907 3060 4427 4601 65 Size: 1183 1395 45 219 153 1367 174 1396 Fragments arranged by size: 1396 1395 1367 1183 219 174 153 45 BsrFI r'CCGG_y Cuts at: 1700 1810 4576 4763 1700 Size: 110 2766 187 2869 Fragments arranged by size: 2869 2766 187 110 BsrGI T'GTAC_A Cuts at: 96 2173 2293 96 Size: 2077 120 3735 Fragments arranged by size: 3735 2077 120 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5932 BssSI C'ACGA_G Cuts at: 3992 5004 3992 Size: 1012 4920 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5932 BstBI TT'CG_AA Cuts at: 4736 4736 Size: 5932 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5932 BstXI CCAn_nnnn'nTGG Cuts at: 2534 2785 2534 Size: 251 5681 BstYI r'GATC_y Cuts at: 1972 2973 3262 3629 4014 4031 5483 5569 5581 Size: 1001 289 367 385 17 1452 86 12 Cuts at: 5581 5927 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1001 385 367 346 289 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3004 513 Size: 552 1939 3441 Fragments arranged by size: 3441 1939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5193 Fragments arranged by size: 5193 683 56 BtsI GCAGTG_nn' Cuts at: 2245 3448 4242 4262 2245 Size: 1203 794 20 3915 Fragments arranged by size: 3915 1203 794 20 ClaI AT'CG_AT Cuts at: 1554 2575 1554 Size: 1021 4911 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5897 DraI TTT'AAA Cuts at: 3236 3236 Size: 5932 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4939 809 Size: 507 3623 1802 Fragments arranged by size: 3623 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2685 3078 3379 Size: 54 1425 50 275 18 855 393 301 Cuts at: 3379 4268 5897 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 855 393 301 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3379 1537 Size: 275 1567 4090 Fragments arranged by size: 4090 1567 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2601 3862 1353 Size: 1248 1261 3423 Fragments arranged by size: 3423 1261 1248 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4519 4893 5039 387 Size: 1064 138 2930 374 146 1280 Fragments arranged by size: 2930 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2218 2240 2261 2347 2721 2761 2859 Size: 393 586 22 21 86 374 40 98 Cuts at: 2859 3995 4592 5379 5647 1239 Size: 1136 597 787 268 1524 Fragments arranged by size: 1524 1136 787 597 586 393 374 268 98 86 40 22 21 EcoO109I rG'GnC_Cy Cuts at: 2538 3217 2538 Size: 679 5253 EcoRI G'AATT_C Cuts at: 3241 3619 3241 Size: 378 5554 EcoRV GAT'ATC Cuts at: 2385 2385 Size: 5932 FspI TGC'GCA Cuts at: 4438 4438 Size: 5932 HaeII r_GCGC'y Cuts at: 1678 3063 5079 1678 Size: 1385 2016 2531 Fragments arranged by size: 2531 2016 1385 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2632 2664 4573 4605 4647 Size: 32 135 32 1060 32 1909 32 42 Cuts at: 4647 4679 1373 Size: 32 2626 Fragments arranged by size: 2626 1909 1060 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3276 3522 669 Size: 988 281 1338 246 3079 Fragments arranged by size: 3079 1338 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5932 HpaI GTT'AAC Cuts at: 3522 3522 Size: 5932 KasI G'GCGC_C Cuts at: 3059 3059 Size: 5932 KpnI G_GTAC'C Cuts at: 3257 3257 Size: 5932 MfeI C'AATT_G Cuts at: 3531 3531 Size: 5932 MluI A'CGCG_T Cuts at: 5808 5808 Size: 5932 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3090 3106 5046 5230 1305 Size: 1785 16 1940 184 2007 Fragments arranged by size: 2007 1940 1785 184 16 MscI TGG'CCA Cuts at: 10 64 1489 1832 3080 10 Size: 54 1425 343 1248 2862 Fragments arranged by size: 2862 1425 1248 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2423 3074 3890 4249 4408 518 Size: 648 1257 651 816 359 159 2042 Fragments arranged by size: 2042 1257 816 651 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3092 3348 4025 5173 5418 1606 Size: 1486 256 677 1148 245 2120 Fragments arranged by size: 2120 1486 1148 677 256 245 NarI GG'CG_CC Cuts at: 3060 3060 Size: 5932 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5380 NdeI CA'TA_TG Cuts at: 387 387 Size: 5932 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5932 NotI GC'GGCC_GC Cuts at: 3379 3379 Size: 5932 NspI r_CATG'y Cuts at: 1132 1229 2180 2988 3290 4835 1132 Size: 97 951 808 302 1545 2229 Fragments arranged by size: 2229 1545 951 808 302 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5932 PciI A'CATG_T Cuts at: 2176 2984 4831 2176 Size: 808 1847 3277 Fragments arranged by size: 3277 1847 808 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5932 PfoI T'CCnGG_A Cuts at: 2037 2037 Size: 5932 PmeI GTTT'AAAC Cuts at: 3236 3236 Size: 5932 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4001 4033 5514 5546 4001 Size: 32 1481 32 4387 Fragments arranged by size: 4387 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3217 3217 Size: 5932 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5932 PsiI TTA'TAA Cuts at: 3502 3502 Size: 5932 PstI C_TGCA'G Cuts at: 830 3284 830 Size: 2454 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5932 PvuII CAG'CTG Cuts at: 3092 3092 Size: 5932 SalI G'TCGA_C Cuts at: 1936 3274 1936 Size: 1338 4594 SbfI CC_TGCA'GG Cuts at: 3284 3284 Size: 5932 ScaI AGT'ACT Cuts at: 4180 5892 4180 Size: 1712 4220 SexAI A'CCwGG_T Cuts at: 2475 3013 2475 Size: 538 5394 SfcI C'TryA_G Cuts at: 826 1045 2031 2556 3280 4415 5096 5287 826 Size: 219 986 525 724 1135 681 191 1471 Fragments arranged by size: 1471 1135 986 724 681 525 219 191 SfoI GGC'GCC Cuts at: 3061 3061 Size: 5932 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5932 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5932 SmaI CCC'GGG Cuts at: 1872 3259 1872 Size: 1387 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2102 4036 4937 5199 820 Size: 197 926 159 1934 901 262 1553 Fragments arranged by size: 1934 1553 926 901 262 197 159 SnaBI TAC'GTA Cuts at: 493 3723 493 Size: 3230 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5932 SphI G_CATG'C Cuts at: 1132 3290 1132 Size: 2158 3774 SspI AAT'ATT Cuts at: 5 52 2490 3856 5 Size: 47 2438 1366 2081 Fragments arranged by size: 2438 2081 1366 47 StuI AGG'CCT Cuts at: 1517 2498 3030 1517 Size: 981 532 4419 Fragments arranged by size: 4419 981 532 StyI C'CwwG_G Cuts at: 513 1065 3371 513 Size: 552 2306 3074 Fragments arranged by size: 3074 2306 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2435 2895 3959 3976 4129 4314 4759 Size: 1255 855 460 1064 17 153 185 445 Cuts at: 4759 325 Size: 1498 Fragments arranged by size: 1498 1255 1064 855 460 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2173 2293 4178 Size: 274 80 33 51 1443 196 120 1885 Cuts at: 4178 5890 96 Size: 1712 138 Fragments arranged by size: 1885 1712 1443 274 196 138 120 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2835 2867 5892 5924 2835 Size: 32 3025 32 2843 Fragments arranged by size: 3025 2843 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5932 XbaI T'CTAG_A Cuts at: 3268 3268 Size: 5932 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2984 3011 3073 2984 Size: 27 62 5843 Fragments arranged by size: 5843 62 27 XhoI C'TCGA_G Cuts at: 1943 2102 1943 Size: 159 5773 XmaI C'CCGG_G Cuts at: 1870 3257 1870 Size: 1387 4545 XmnI GAAnn'nnTTC Cuts at: 4061 4061 Size: 5932 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3740 276 Size: 53 83 186 936 2206 2468 Fragments arranged by size: 2468 2206 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SrfI SwaI