Restriction map for
pFN21AB5687
##################################
# NO CUT ENZYMES ( 159 enzymes) #
##################################
AatII Acc65I AccII AccIII AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AvaII AviII AvrII BanI BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfrI BglII BlnI BpmI
BsaAI BsaWI BseAI BseRI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I Cfr10I Cfr13I
ClaI CpoI DraI DraII DraIII
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HapII HincII
HindII HindIII HpaI HpaII KasI
KpnI KspI MaeIII MfeI MluI
MroI MspA1I MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI PstI PvuI PvuII
RcaI RsrII SacII SalI Sau96I
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tsp45I Van91I XbaI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccI 314 GTAGAC GT^[AC][GT]AC
AclI 610 AACGTT AA^CGTT
AcsI 367 GAATTT [GA]^AATT[CT]
AcyI 462 GACGCC G[GA]^CG[CT]C
AfaI 431 GTAC GT^AC
ApoI 367 GAATTT [GA]^AATT[CT]
AspHI 442 GAGCTC G[AT]GC[AT]^C
AvaI 145 CCCGAG C^[CT]CG[GA]G
BalI 494 TGGCCA TGG^CCA
BamHI 48 GGATCC G^GATCC
BanII 442 GAGCTC G[GA]GC[CT]^C
BbsI 124 GTCTTC GAAGAC(2/6)
BfaI 662 CTAG C^TAG
BglI 577 GCCACCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 530 GCTCAGC GC^T[ACGT]AGC
BmyI 442 GAGCTC G[AGT]GC[ACT]^C
Bpu1102I 530 GCTCAGC GC^T[ACGT]AGC
BpuAI 124 GTCTTC GAAGAC(2/6)
BsaBI 49 GATCCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 462 GACGCC G[GA]^CG[CT]C
BsaI 322 GAGACC GGTCTC(1/5)
BsgI 720 CTGCAC GTGCAG(16/14)
BsiHKAI 442 GAGCTC G[AT]GC[AT]^C
BsmFI 227 GGGAC GGGAC(10/14)
BsmI 526 GAATGC GAATGC(1/-1)
BsoBI 145 CCCGAG C^[CT]CG[GA]G
Bsp1286I 442 GAGCTC G[AGT]GC[ACT]^C
BstF5I 285 GGATG GGATG(2/0)
BstYI 48 GGATCC [GA]^GATC[CT]
Cac8I 16 GCCAGC GC[ACGT]^[ACGT]GC
CelII 530 GCTCAGC GC^T[ACGT]AGC
CfoI 112 GCGC GCG^C
DdeI 531 CTCAG C^T[ACGT]AG
DrdI 462 GACGCCAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FokI 285 GGATG GGATG(9/13)
HhaI 112 GCGC GCG^C
Hin1I 462 GACGCC G[GA]^CG[CT]C
HinP1I 112 GCGC G^CGC
HinfI 280 GATTC G^A[ACGT]TC
HphI 355 GGTGA GGTGA(8/7)
MaeI 662 CTAG C^TAG
MamI 49 GATCCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 48 GGATCC [GA]^GATC[CT]
MluNI 494 TGGCCA TGG^CCA
MscI 494 TGGCCA TGG^CCA
MseI 614 TTAA T^TAA
MslI 723 CACTGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NlaIV 48 GGATCC GG[ACGT]^[ACGT]CC
Psp1406I 610 AACGTT AA^CGTT
RsaI 431 GTAC GT^AC
SacI 442 GAGCTC GAGCT^C
SfaNI 286 GATGC GCATC(5/9)
SfcI 161 CTACAG C^T[GA][CT]AG
TfiI 280 GATTC G^A[AT]TC
Tru9I 614 TTAA T^TAA
XcmI 383 CCAAAATGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 48 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AlwI 49 GATCC GGATC(4/5)
48 GGATC
AspI 253 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
331 GACAGTGTC
BsrI 185 CCAGT ACTGG(1/-1)
466 CCAGT
DpnII 49 GATC ^GATC
412 GATC
EaeI 182 CGGCCA [CT]^GGCC[GA]
494 TGGCCA
EarI 11 GAAGAG CTCTTC(1/4)
714 GAAGAG
EcoT14I 131 CCAAGG C^C[AT][AT]GG
581 CCTTGG
HaeIII 183 GGCC GG^CC
495 GGCC
HgaI 462 GACGC GACGC(5/10)
554 GCGTC
Ksp632I 11 GAAGAG CTCTTC(1/4)
714 GAAGAG
MboI 49 GATC ^GATC
412 GATC
MnlI 267 CCTC CCTC(7/6)
339 CCTC
MwoI 487 GCAAATATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
577 GCCACCTTGGC
NdeII 49 GATC ^GATC
412 GATC
NspI 586 GCATGT [GA]CATG^[CT]
656 ACATGC
PflFI 253 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
331 GACAGTGTC
SapI 11 GAAGAGC GCTCTTC(1/4)
714 GAAGAGC
Sau3AI 49 GATC ^GATC
412 GATC
StyI 131 CCAAGG C^C[AT][AT]GG
581 CCTTGG
TaqI 197 TCGA T^CGA
524 TCGA
Tth111I 253 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
331 GACAGTGTC
TthHB8I 197 TCGA T^CGA
524 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AciI AluI BbvI BsaJI BsiYI
BslI BsmAI BssKI BstNI EcoRII
Fnu4HI ItaI MaeII MboII MvaI
NlaIII ScrFI TseI Tsp509I TspRI