################################## # NO CUT ENZYMES ( 177 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanII BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiHKAI BsiWI BsmAI BsmBI BsmI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstUI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DpnII DraI DraII DraIII DrdI DsaI Eam1105I EarI Eco47III Eco81I EcoNI EcoO109I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI Ksp632I KspI MaeI MamI MboI MfeI MflI MluI MluNI MroI MscI MseI MspA1I MunI MvnI NcoI NdeI NdeII NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tru9I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AcyI 109 GGCGCC G[GA]^CG[CT]C AluI 480 AGCT AG^CT AvaII 98 GGACC G^G[AT]CC BanI 109 GGCGCC G^G[CT][GA]CC BbeI 109 GGCGCC GGCGC^C BbsI 331 GTCTTC GAAGAC(2/6) BpmI 124 CTGGAG CTGGAG(16/14) BpuAI 331 GTCTTC GAAGAC(2/6) BsaHI 109 GGCGCC G[GA]^CG[CT]C BsmFI 22 GTCCC GGGAC(10/14) BsrDI 388 CATTGC GCAATG(2/0) BstAPI 378 GCATGATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 66 GGTCACC G^GT[ACGT]ACC BstPI 66 GGTCACC G^GT[ACGT]ACC CfoI 110 GCGC GCG^C DdeI 421 CTGAG C^T[ACGT]AG EaeI 5 CGGCCG [CT]^GGCC[GA] EagI 5 CGGCCG C^GGCCG EclXI 5 CGGCCG C^GGCCG Eco52I 5 CGGCCG C^GGCCG EcoO65I 66 GGTCACC G^GT[ACGT]ACC HaeII 109 GGCGCC [GA]GCGC^[CT] HgaI 16 GCGTC GACGC(5/10) HhaI 110 GCGC GCG^C Hin1I 109 GGCGCC G[GA]^CG[CT]C HinP1I 110 GCGC G^CGC HinfI 129 GATTC G^A[ACGT]TC HphI 68 TCACC GGTGA(8/7) KasI 109 GGCGCC G^GCGCC MaeII 446 ACGT A^CGT MslI 204 CATGTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 109 GGCGCC GG^CGCC NspI 251 GCATGT [GA]CATG^[CT] SfaNI 283 GATGC GCATC(5/9) SfoI 109 GGCGCC GGC^GCC SmaI 62 CCCGGG CCC^GGG TfiI 129 GATTC G^A[AT]TC XmaCI 62 CCCGGG C^CCGGG XmaI 62 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AvaI 62 CCCGGG C^[CT]CG[GA]G 105 CTCGGG BsaJI 61 CCCCGG C^C[ACGT][ACGT]GG 62 CCCGGG BseRI 165 CTCCTC GAGGAG(10/8) 238 CTCCTC BsiEI 5 CGGCCG CG[GA][CT]^CG 523 CGACCG BsiYI 240 CCTCTTTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 401 CCATAACCGGG BslI 240 CCTCTTTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 401 CCATAACCGGG BsoBI 62 CCCGGG C^[CT]CG[GA]G 105 CTCGGG BsrFI 7 GCCGGC [GA]^CCGG[CT] 79 GCCGGC BsrI 290 ACTGG ACTGG(1/-1) 432 CCAGT BstNI 123 CCTGG CC^[AT]GG 300 CCTGG Cfr10I 7 GCCGGC [GA]^CCGG[CT] 79 GCCGGC Cfr13I 82 GGCCC G^G[ACGT]CC 98 GGACC EcoRII 123 CCTGG ^CC[AT]GG 300 CCTGG HaeIII 6 GGCC GG^CC 82 GGCC MaeIII 67 GTCAC ^GT[ACGT]AC 217 GTGAC MboII 515 GAAGA GAAGA(8/7) 332 TCTTC MvaI 123 CCTGG CC^[AT]GG 300 CCTGG MwoI 7 GCCGGCGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 378 GCATGATTTGC NaeI 7 GCCGGC GCC^GGC 79 GCCGGC NgoMIV 7 GCCGGC G^CCGGC 79 GCCGGC Sau96I 82 GGCCC G^G[ACGT]CC 98 GGACC Tsp45I 67 GTCAC ^GT[GC]AC 217 GTGAC Tsp509I 279 AATT ^AATT 459 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 19 enzymes) # ######################################### AciI BbvI BcnI BssKI BstF5I Cac8I FauI Fnu4HI FokI HapII HpaII ItaI MnlI MspI NciI NlaIII NlaIV ScrFI TseI