################################## # NO CUT ENZYMES ( 186 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BamHI BanII BbeI BbrPI BbvCI BbvI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BseRI BsiEI BsiHKAI BsiWI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI Fnu4HI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HpaI HphI ItaI KasI KpnI Ksp632I KspI MamI MfeI MflI MluI MspA1I MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfcI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI SwaI TseI Tsp45I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AciI 566 CCGC CCGC(-3/-1) AfaI 59 GTAC GT^AC AlwI 104 GATCC GGATC(4/5) BalI 116 TGGCCA TGG^CCA BanI 205 GGTGCC G^G[CT][GA]CC BbsI 471 GAAGAC GAAGAC(2/6) BfaI 83 CTAG C^TAG BpuAI 471 GAAGAC GAAGAC(2/6) BsgI 179 GTGCAG GTGCAG(16/14) BsrDI 488 GCAATG GCAATG(2/0) BsrI 462 ACTGG ACTGG(1/-1) BssKI 528 CCTGG ^CC[ACGT]GG BstBI 188 TTCGAA TT^CGAA BstF5I 419 GGATG GGATG(2/0) BstNI 528 CCTGG CC^[AT]GG Cfr13I 250 GGGCC G^G[ACGT]CC EaeI 116 TGGCCA [CT]^GGCC[GA] EcoRII 528 CCTGG ^CC[AT]GG EcoT14I 87 CCTTGG C^C[AT][AT]GG FokI 419 GGATG GGATG(9/13) HindIII 456 AAGCTT A^AGCTT MaeI 83 CTAG C^TAG MaeII 278 ACGT A^CGT MaeIII 428 GTTAC ^GT[ACGT]AC MluNI 116 TGGCCA TGG^CCA MscI 116 TGGCCA TGG^CCA MslI 391 CACAGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvaI 528 CCTGG CC^[AT]GG NlaIII 397 CATG CATG^ NlaIV 205 GGTGCC GG[ACGT]^[ACGT]CC NspI 396 GCATGC [GA]CATG^[CT] NspV 188 TTCGAA TT^CGAA PleI 574 GACTC GAGTC(4/5) RsaI 59 GTAC GT^AC Sau96I 250 GGGCC G^G[ACGT]CC ScaI 58 AGTACT AGT^ACT ScrFI 528 CCTGG CC^[ACGT]GG SfaNI 481 GATGC GCATC(5/9) SfuI 188 TTCGAA TT^CGAA SphI 396 GCATGC GCATG^C StuI 240 AGGCCT AGG^CCT StyI 87 CCTTGG C^C[AT][AT]GG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccIII 315 TCCGGA T^CCGGA 416 TCCGGA AcsI 184 GAATTT [GA]^AATT[CT] 325 GAATTT AluI 145 AGCT AG^CT 457 AGCT ApoI 184 GAATTT [GA]^AATT[CT] 325 GAATTT BsaJI 87 CCTTGG C^C[ACGT][ACGT]GG 230 CCGAGG BsaWI 315 TCCGGA [AT]^CCGG[AT] 416 TCCGGA BseAI 315 TCCGGA T^CCGGA 416 TCCGGA BspEI 315 TCCGGA T^CCGGA 416 TCCGGA BstXI 107 CCAGCACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 285 CCAGCAAGATGG DpnII 104 GATC ^GATC 373 GATC HapII 316 CCGG C^CGG 417 CCGG HpaII 316 CCGG C^CGG 417 CCGG MboI 104 GATC ^GATC 373 GATC MboII 322 GAAGA GAAGA(8/7) 471 GAAGA MroI 315 TCCGGA T^CCGGA 416 TCCGGA MseI 409 TTAA T^TAA 583 TTAA MspI 316 CCGG C^CGG 417 CCGG NdeII 104 GATC ^GATC 373 GATC Sau3AI 104 GATC ^GATC 373 GATC TaqI 125 TCGA T^CGA 189 TCGA TfiI 79 GATTC G^A[AT]TC 133 GATTC Tru9I 409 TTAA T^TAA 583 TTAA TspRI 111 CACTG CA[GC]TG[ACGT][ACGT]^ 517 CAGTG TthHB8I 125 TCGA T^CGA 189 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 6 enzymes) # ######################################### Cac8I DdeI HaeIII HinfI MnlI Tsp509I