################################## # NO CUT ENZYMES ( 102 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BbrPI BbvCI BcgI BciVI BclI BfrI BglII BlnI BsaAI BsiWI BsmBI BsmI Bsp1407I BspDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FspI HindIII HpaI KpnI MfeI MluI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SalI SapI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SspBI SspI SwaI Tth111I Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### Asp700I 432 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BamHI 542 GGATCC G^GATCC BbeI 119 GGCGCC GGCGC^C BsiEI 1083 CGACCG CG[GA][CT]^CG BspLU11I 434 ACATGT A^CATGT BspMI 1576 ACCTGC ACCTGC(4/8) BsrBI 1606 GAGCGG CCGCTC(-3/-3) BstAPI 996 GCAGGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC EarI 1536 GAAGAG CTCTTC(1/4) FseI 1272 GGCCGGCC GGCCGG^CC HaeII 119 GGCGCC [GA]GCGC^[CT] HgaI 266 GCGTC GACGC(5/10) KasI 119 GGCGCC G^GCGCC Ksp632I 1536 GAAGAG CTCTTC(1/4) KspI 984 CCGCGG CCGC^GG MseI 1382 TTAA T^TAA NaeI 1273 GCCGGC GCC^GGC NarI 119 GGCGCC GG^CGCC NgoMIV 1273 GCCGGC G^CCGGC PpuMI 112 AGGACCC [GA]G^G[AT]CC[CT] PshAI 1671 GACCAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 1256 CAGCTG CAG^CTG SacII 984 CCGCGG CCGC^GG SbfI 993 CCTGCAGG CCTGCA^GG SfoI 119 GGCGCC GGC^GCC SmaI 1445 CCCGGG CCC^GGG SmlI 108 CTTGAG C^T[CT][GA]AG SphI 9 GCATGC GCATG^C Sse8387I 993 CCTGCAGG CCTGCA^GG StuI 1550 AGGCCT AGG^CCT TaqI 1082 TCGA T^CGA TfiI 966 GAATC G^A[AT]TC Tru9I 1382 TTAA T^TAA Tsp509I 138 AATT ^AATT TthHB8I 1082 TCGA T^CGA XmaCI 1445 CCCGGG C^CCGGG XmaI 1445 CCCGGG C^CCGGG XmnI 432 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 41 enzymes) # ################################### AccIII 223 TCCGGA T^CCGGA 428 TCCGGA AcyI 119 GGCGCC G[GA]^CG[CT]C 265 GGCGTC AfaI 282 GTAC GT^AC 474 GTAC AflIII 35 ACGTGT A^C[GA][CT]GT 434 ACATGT ApaI 728 GGGCCC GGGCC^C 1569 GGGCCC AvaI 616 CCCGAG C^[CT]CG[GA]G 1445 CCCGGG BalI 1016 TGGCCA TGG^CCA 1206 TGGCCA BbsI 676 GTCTTC GAAGAC(2/6) 976 GTCTTC BfaI 254 CTAG C^TAG 451 CTAG BlpI 401 GCTCAGC GC^T[ACGT]AGC 1031 GCTCAGC Bpu1102I 401 GCTCAGC GC^T[ACGT]AGC 1031 GCTCAGC BpuAI 676 GTCTTC GAAGAC(2/6) 976 GTCTTC BsaBI 186 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 543 GATCCTCATC BsaHI 119 GGCGCC G[GA]^CG[CT]C 265 GGCGTC BsaWI 223 TCCGGA [AT]^CCGG[AT] 428 TCCGGA BseAI 223 TCCGGA T^CCGGA 428 TCCGGA BsoBI 616 CCCGAG C^[CT]CG[GA]G 1445 CCCGGG BspEI 223 TCCGGA T^CCGGA 428 TCCGGA BspHI 395 TCATGA T^CATGA 770 TCATGA BsrDI 825 CATTGC GCAATG(2/0) 1341 CATTGC BsrFI 1085 ACCGGC [GA]^CCGG[CT] 1273 GCCGGC BstYI 542 GGATCC [GA]^GATC[CT] 836 AGATCC CelII 401 GCTCAGC GC^T[ACGT]AGC 1031 GCTCAGC Cfr10I 1085 ACCGGC [GA]^CCGG[CT] 1273 GCCGGC Hin1I 119 GGCGCC G[GA]^CG[CT]C 265 GGCGTC HincII 1183 GTCAAC GT[CT]^[GA]AC 1348 GTTGAC HindII 1183 GTCAAC GT[CT]^[GA]AC 1348 GTTGAC MaeI 254 CTAG C^TAG 451 CTAG MamI 186 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 543 GATCCTCATC MflI 542 GGATCC [GA]^GATC[CT] 836 AGATCC MluNI 1016 TGGCCA TGG^CCA 1206 TGGCCA MroI 223 TCCGGA T^CCGGA 428 TCCGGA MscI 1016 TGGCCA TGG^CCA 1206 TGGCCA NcoI 692 CCATGG C^CATGG 1286 CCATGG NspI 9 GCATGC [GA]CATG^[CT] 434 ACATGT PstI 994 CTGCAG CTGCA^G 1238 CTGCAG RcaI 395 TCATGA T^CATGA 770 TCATGA RsaI 282 GTAC GT^AC 474 GTAC SfcI 994 CTGCAG C^T[GA][CT]AG 1238 CTGCAG XcmI 561 CCAGGAGGAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1121 CCACCATCAACATGG XhoII 542 GGATCC [GA]^GATC[CT] 836 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 77 enzymes) # ######################################### AccII AciI AluI AlwI AlwNI AspHI AvaII BanI BanII BbvI BcnI BglI BmyI BpmI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI SacI Sau3AI Sau96I ScrFI SfaNI StyI TseI Tsp45I TspRI