(Circular) (Six-base) MAPSORT of: pFN21AE6417.seq Check: 1135 from: 1 to: 6385 pFN21AE6417 6385 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3590 3590 Size: 6385 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4195 278 Size: 53 83 186 936 2659 2468 Fragments arranged by size: 2659 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3728 1937 Size: 1791 4594 Acc65I G'GTAC_C Cuts at: 3706 3706 Size: 6385 AclI AA'CG_TT Cuts at: 1563 4512 4885 1563 Size: 2949 373 3063 Fragments arranged by size: 3063 2949 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2207 4448 5832 6100 1632 Size: 575 2241 1384 268 1917 Fragments arranged by size: 2241 1917 1384 575 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6188 AflIII A'CryG_T Cuts at: 1203 2040 2439 5284 6261 1203 Size: 837 399 2845 977 1327 Fragments arranged by size: 2845 1327 977 837 399 AgeI A'CCGG_T Cuts at: 5216 5216 Size: 6385 AhdI GACnn_n'nnGTC Cuts at: 5114 5114 Size: 6385 AleI CACnn'nnGTG Cuts at: 1166 2903 3311 1166 Size: 1737 408 4240 Fragments arranged by size: 4240 1737 408 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2684 1184 Size: 1500 4885 AlwNI CAG_nnn'CTG Cuts at: 2751 3007 3242 4128 5700 2751 Size: 256 235 886 1572 3436 Fragments arranged by size: 3436 1572 886 256 235 ApaI G_GGCC'C Cuts at: 2737 3578 2737 Size: 841 5544 ApaLI G'TGCA_C Cuts at: 4442 5598 4442 Size: 1156 5229 ApoI r'AATT_y Cuts at: 1493 3694 3920 4072 6181 1493 Size: 2201 226 152 2109 1697 Fragments arranged by size: 2201 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4939 160 Size: 872 3907 1606 Fragments arranged by size: 3907 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6385 AvaI C'yCGr_G Cuts at: 1870 1943 2621 3450 3710 1870 Size: 73 678 829 260 4545 Fragments arranged by size: 4545 829 678 260 73 BamHI G'GATC_C Cuts at: 2547 3715 4082 2547 Size: 1168 367 4850 Fragments arranged by size: 4850 1168 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2008 2124 2521 2648 3122 Size: 325 217 627 221 116 397 127 474 Cuts at: 3122 3461 3706 5161 618 Size: 339 245 1455 1842 Fragments arranged by size: 1842 1455 627 474 397 339 325 245 221 217 127 116 BanII G_rGCy'C Cuts at: 1391 2172 2408 2737 2931 3364 3567 3578 3704 Size: 781 236 329 194 433 203 11 126 Cuts at: 3704 1391 Size: 4072 Fragments arranged by size: 4072 781 433 329 236 203 194 126 11 BbeI G_GCGC'C Cuts at: 2128 2128 Size: 6385 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2674 2974 928 Size: 971 775 300 4339 Fragments arranged by size: 4339 971 775 300 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4576 4610 1903 Size: 34 2639 34 3678 Fragments arranged by size: 3678 2639 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4277 5487 4277 Size: 1210 5175 BclI T'GATC_A Cuts at: 1548 1548 Size: 6385 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2024 2103 2268 Size: 107 122 71 631 605 352 79 165 Cuts at: 2268 4996 136 Size: 2728 1525 Fragments arranged by size: 2728 1525 631 605 352 165 122 107 79 71 BglII A'GATC_T Cuts at: 6380 6380 Size: 6385 BlpI GC'TnA_GC Cuts at: 2407 3037 3802 2407 Size: 630 765 4990 Fragments arranged by size: 4990 765 630 Bme1580I G_kGCm'C Cuts at: 1790 2651 2737 3578 4446 5602 1790 Size: 861 86 841 868 1156 2573 Fragments arranged by size: 2573 1156 868 861 841 86 BmgBI CAC'GTC Cuts at: 1318 2041 3595 1318 Size: 723 1554 4108 Fragments arranged by size: 4108 1554 723 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2492 3367 4116 Size: 416 53 83 399 24 1075 875 749 Cuts at: 4116 5074 442 Size: 958 1753 Fragments arranged by size: 1753 1075 958 875 749 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6385 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2596 2596 Size: 6385 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2184 2200 2867 3016 3092 3488 3692 5045 Size: 945 16 667 149 76 396 204 1353 Cuts at: 5045 1239 Size: 2579 Fragments arranged by size: 2579 1353 945 667 396 204 149 76 16 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2134 4510 5375 5673 2134 Size: 2376 865 298 2846 Fragments arranged by size: 2846 2376 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2218 2341 2366 3479 3668 5048 882 Size: 617 719 123 25 1113 189 1380 2219 Fragments arranged by size: 2219 1380 1113 719 617 189 123 25 BsaAI yAC'GTr Cuts at: 493 1204 4176 493 Size: 711 2972 2702 Fragments arranged by size: 2972 2702 711 BsaBI GATnn'nnATC Cuts at: 2195 2552 6379 2195 Size: 357 3827 2201 Fragments arranged by size: 3827 2201 357 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2125 2271 4192 4574 Size: 53 83 186 936 592 146 1921 382 Cuts at: 4574 5209 275 Size: 635 1451 Fragments arranged by size: 1921 1451 936 635 592 382 186 146 83 53 BsaWI w'CCGG_w Cuts at: 2228 2433 4818 5216 5490 5637 6048 2228 Size: 205 2385 398 274 147 411 2565 Fragments arranged by size: 2565 2385 411 398 274 205 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6160 Fragments arranged by size: 6160 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2051 2090 2529 2543 2585 3019 3635 1186 Size: 865 39 439 14 42 434 616 3936 Fragments arranged by size: 3936 865 616 439 434 42 39 14 BseYI C'CCAG_C Cuts at: 2446 2671 2710 2881 3394 3522 5588 6084 2446 Size: 225 39 171 513 128 2066 496 2747 Fragments arranged by size: 2747 2066 513 496 225 171 128 39 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2065 2609 3634 1163 Size: 573 329 544 1025 3914 Fragments arranged by size: 3914 1025 573 544 329 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3091 3835 4596 5624 1540 Size: 275 186 1090 744 761 1028 2301 Fragments arranged by size: 2301 1090 1028 761 744 275 186 BsiHKAI G_wGCw'C Cuts at: 2172 2408 2922 3306 3567 4446 4531 5602 2172 Size: 236 514 384 261 879 85 1071 2955 Fragments arranged by size: 2955 1071 879 514 384 261 236 85 BsmI GAATG_Cn' Cuts at: 3896 3989 3896 Size: 93 6292 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2172 2408 2651 2737 2922 2931 3306 Size: 399 382 236 243 86 185 9 375 Cuts at: 3306 3364 3567 3578 3704 4446 4531 5602 1391 Size: 58 203 11 126 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 382 375 243 236 203 185 126 86 85 58 11 9 BspEI T'CCGG_A Cuts at: 2228 2433 6048 2228 Size: 205 3615 2565 Fragments arranged by size: 3615 2565 205 BspHI T'CATG_A Cuts at: 2400 2775 4274 6004 2400 Size: 375 1499 1730 2781 Fragments arranged by size: 2781 1730 1499 375 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3590 3740 844 Size: 2746 150 3489 Fragments arranged by size: 3489 2746 150 BsrBI CCG'CTC Cuts at: 1394 1823 3613 4272 1394 Size: 429 1790 659 3507 Fragments arranged by size: 3507 1790 659 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2829 3345 4880 5054 65 Size: 1183 1581 516 1535 174 1396 Fragments arranged by size: 1581 1535 1396 1183 516 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3090 3278 5029 5216 1700 Size: 110 1280 188 1751 187 2869 Fragments arranged by size: 2869 1751 1280 188 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6385 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6385 BssSI C'ACGA_G Cuts at: 4445 5457 4445 Size: 1012 5373 BstAPI GCAn_nnn'nTGC Cuts at: 794 3007 794 Size: 2213 4172 BstBI TT'CG_AA Cuts at: 5189 5189 Size: 6385 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6385 BstXI CCAn_nnnn'nTGG Cuts at: 2162 2308 3136 3530 2162 Size: 146 828 394 5017 Fragments arranged by size: 5017 828 394 146 BstYI r'GATC_y Cuts at: 1972 2547 2841 3715 4082 4467 4484 5936 6022 Size: 575 294 874 367 385 17 1452 86 Cuts at: 6022 6034 6380 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 874 575 385 367 346 294 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2280 2376 2697 2901 2923 2989 Size: 552 939 276 96 321 204 22 66 Cuts at: 2989 3117 3291 3312 513 Size: 128 174 21 3586 Fragments arranged by size: 3586 939 552 321 276 204 174 128 96 66 22 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2670 2998 3082 3163 487 Size: 683 56 1444 328 84 81 3709 Fragments arranged by size: 3709 1444 683 328 84 81 56 BtsI GCAGTG_nn' Cuts at: 3044 3144 3240 3646 3901 4695 4715 3044 Size: 100 96 406 255 794 20 4714 Fragments arranged by size: 4714 794 406 255 100 96 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6385 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2482 2517 529 Size: 35 1918 35 4397 Fragments arranged by size: 4397 1918 35 35 DraI TTT'AAA Cuts at: 3689 3689 Size: 6385 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5392 809 Size: 507 4076 1802 Fragments arranged by size: 4076 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3021 3165 3211 Size: 54 1425 50 275 18 1191 144 46 Cuts at: 3211 3832 4721 6350 8 Size: 621 889 1629 43 Fragments arranged by size: 1629 1425 1191 889 621 275 144 54 50 46 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3832 1537 Size: 275 2020 4090 Fragments arranged by size: 4090 2020 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3536 4315 1353 Size: 2183 779 3423 Fragments arranged by size: 3423 2183 779 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2065 2560 4972 5346 5492 387 Size: 1064 138 476 495 2412 374 146 1280 Fragments arranged by size: 2412 1280 1064 495 476 374 146 138 EcoICRI GAG'CTC Cuts at: 2170 2406 3565 2170 Size: 236 1159 4990 Fragments arranged by size: 4990 1159 236 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2184 2200 2207 2867 3016 3092 3488 Size: 393 552 16 7 660 149 76 396 Cuts at: 3488 3692 4448 5045 5832 6100 1239 Size: 204 756 597 787 268 1524 Fragments arranged by size: 1524 787 756 660 597 552 396 393 268 204 149 76 16 7 EcoO109I rG'GnC_Cy Cuts at: 2118 2533 2733 2734 3575 2118 Size: 415 200 1 841 4928 Fragments arranged by size: 4928 841 415 200 1 EcoRI G'AATT_C Cuts at: 3694 4072 3694 Size: 378 6007 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2902 2902 Size: 6385 FseI GG_CCGG'CC Cuts at: 3282 3282 Size: 6385 FspI TGC'GCA Cuts at: 4891 4891 Size: 6385 HaeII r_GCGC'y Cuts at: 1678 2128 5532 1678 Size: 450 3404 2531 Fragments arranged by size: 3404 2531 450 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2049 2081 5026 5058 5100 Size: 32 135 32 477 32 2945 32 42 Cuts at: 5100 5132 1373 Size: 32 2626 Fragments arranged by size: 2945 2626 477 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3190 3355 3729 3975 669 Size: 988 281 1252 165 374 246 3079 Fragments arranged by size: 3079 1252 988 374 281 246 165 HindIII A'AGCT_T Cuts at: 748 748 Size: 6385 HpaI GTT'AAC Cuts at: 3975 3975 Size: 6385 KasI G'GCGC_C Cuts at: 2124 2124 Size: 6385 KpnI G_GTAC'C Cuts at: 3710 3710 Size: 6385 MfeI C'AATT_G Cuts at: 3984 3984 Size: 6385 MluI A'CGCG_T Cuts at: 6261 6261 Size: 6385 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2403 3416 5499 5683 1305 Size: 1098 1013 2083 184 2007 Fragments arranged by size: 2083 2007 1098 1013 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3023 3213 10 Size: 54 1425 343 1191 190 3182 Fragments arranged by size: 3182 1425 1191 343 190 54 MslI CAynn'nnrTG Cuts at: 518 1166 2306 2330 2666 2903 3134 3311 4343 Size: 648 1140 24 336 237 231 177 1032 Cuts at: 4343 4702 4861 518 Size: 359 159 2042 Fragments arranged by size: 2042 1140 1032 648 359 336 237 231 177 159 24 MspA1I CmG'CkG Cuts at: 1606 2730 2991 3263 3413 3632 3801 4478 5626 Size: 1124 261 272 150 219 169 677 1148 Cuts at: 5626 5871 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 1124 677 272 261 245 219 169 150 NaeI GCC'GGC Cuts at: 3280 3280 Size: 6385 NarI GG'CG_CC Cuts at: 2125 2125 Size: 6385 NcoI C'CATG_G Cuts at: 513 1065 2004 2697 3291 513 Size: 552 939 693 594 3607 Fragments arranged by size: 3607 939 693 594 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6385 NgoMIV G'CCGG_C Cuts at: 3278 3278 Size: 6385 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6385 NotI GC'GGCC_GC Cuts at: 3832 3832 Size: 6385 NspI r_CATG'y Cuts at: 1132 1229 2018 2443 3743 5288 1132 Size: 97 789 425 1300 1545 2229 Fragments arranged by size: 2229 1545 1300 789 425 97 PasI CC'CwG_GG Cuts at: 1348 2862 3571 1348 Size: 1514 709 4162 Fragments arranged by size: 4162 1514 709 PciI A'CATG_T Cuts at: 2439 5284 2439 Size: 2845 3540 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6385 PfoI T'CCnGG_A Cuts at: 2565 2844 3504 2565 Size: 279 660 5446 Fragments arranged by size: 5446 660 279 PmeI GTTT'AAAC Cuts at: 3689 3689 Size: 6385 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4454 4486 5967 5999 4454 Size: 32 1481 32 4840 Fragments arranged by size: 4840 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2118 2118 Size: 6385 PshAI GACnn'nnGTC Cuts at: 1613 3680 1613 Size: 2067 4318 PsiI TTA'TAA Cuts at: 3955 3955 Size: 6385 PspOMI G'GGCC_C Cuts at: 2733 3574 2733 Size: 841 5544 PstI C_TGCA'G Cuts at: 830 3003 3247 3737 830 Size: 2173 244 490 3478 Fragments arranged by size: 3478 2173 490 244 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6385 PvuII CAG'CTG Cuts at: 3263 3263 Size: 6385 SacI G_AGCT'C Cuts at: 2172 2408 3567 2172 Size: 236 1159 4990 Fragments arranged by size: 4990 1159 236 SacII CC_GC'GG Cuts at: 2992 2992 Size: 6385 SalI G'TCGA_C Cuts at: 1936 3727 1936 Size: 1791 4594 SbfI CC_TGCA'GG Cuts at: 3003 3737 3003 Size: 734 5651 ScaI AGT'ACT Cuts at: 4633 6345 4633 Size: 1712 4673 SfcI C'TryA_G Cuts at: 826 1045 2999 3243 3733 4868 5549 5740 826 Size: 219 1954 244 490 1135 681 191 1471 Fragments arranged by size: 1954 1471 1135 681 490 244 219 191 SfoI GGC'GCC Cuts at: 2126 2126 Size: 6385 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6385 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6385 SmaI CCC'GGG Cuts at: 1872 3452 3712 1872 Size: 1580 260 4545 Fragments arranged by size: 4545 1580 260 SmlI C'TyrA_G Cuts at: 820 1017 1943 2113 4489 5390 5652 820 Size: 197 926 170 2376 901 262 1553 Fragments arranged by size: 2376 1553 926 901 262 197 170 SnaBI TAC'GTA Cuts at: 493 4176 493 Size: 3683 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6385 SphI G_CATG'C Cuts at: 1132 2018 3743 1132 Size: 886 1725 3774 Fragments arranged by size: 3774 1725 886 SspI AAT'ATT Cuts at: 5 52 4309 5 Size: 47 4257 2081 Fragments arranged by size: 4257 2081 47 StuI AGG'CCT Cuts at: 1517 3557 1517 Size: 2040 4345 StyI C'CwwG_G Cuts at: 513 1065 2004 2514 2697 3291 3824 513 Size: 552 939 510 183 594 533 3074 Fragments arranged by size: 3074 939 594 552 533 510 183 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2364 2711 2761 3110 3386 4412 4429 Size: 1255 784 347 50 349 276 1026 17 Cuts at: 4429 4582 4767 5212 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 1026 784 445 349 347 276 185 153 50 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4631 6343 96 Size: 274 80 33 51 1443 2654 1712 138 Fragments arranged by size: 2654 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6345 6377 6345 Size: 32 6353 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6385 XbaI T'CTAG_A Cuts at: 3721 3721 Size: 6385 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2573 3133 2573 Size: 560 5825 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6385 XmaI C'CCGG_G Cuts at: 1870 3450 3710 1870 Size: 1580 260 4545 Fragments arranged by size: 4545 1580 260 XmnI GAAnn'nnTTC Cuts at: 2441 4514 2441 Size: 2073 4312 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4193 276 Size: 53 83 186 936 2659 2468 Fragments arranged by size: 2659 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SanDI SapI SexAI SfiI SrfI SwaI