(Linear) (Six-base) MAPSORT of: KIEE6417.seq Check: 3431 from: 1 to: 1680 KIEE6417.orf With 182 enzymes: SgfI * March 16, 2015 11:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1585 1680 Size: 1585 95 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 202 1680 Size: 202 1478 AflIII A'CryG_T Cuts at: 0 35 434 1680 Size: 35 399 1246 Fragments arranged by size: 1246 399 35 AleI CACnn'nnGTG Cuts at: 0 898 1306 1680 Size: 898 408 374 Fragments arranged by size: 898 408 374 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 679 1680 Size: 679 1001 AlwNI CAG_nnn'CTG Cuts at: 0 746 1002 1237 1680 Size: 746 256 235 443 Fragments arranged by size: 746 443 256 235 ApaI G_GGCC'C Cuts at: 0 732 1573 1680 Size: 732 841 107 Fragments arranged by size: 841 732 107 AvaI C'yCGr_G Cuts at: 0 616 1445 1680 Size: 616 829 235 Fragments arranged by size: 829 616 235 BamHI G'GATC_C Cuts at: 0 542 1680 Size: 542 1138 BanI G'GyrC_C Cuts at: 0 3 119 516 643 1117 1456 1680 Size: 3 116 397 127 474 339 224 Fragments arranged by size: 474 397 339 224 127 116 3 BanII G_rGCy'C Cuts at: 0 167 403 732 926 1359 1562 1573 1680 Size: 167 236 329 194 433 203 11 107 Fragments arranged by size: 433 329 236 203 194 167 107 11 BbeI G_GCGC'C Cuts at: 0 123 1680 Size: 123 1557 BbsI GAAGACnn'nnnn_ Cuts at: 0 669 969 1680 Size: 669 300 711 Fragments arranged by size: 711 669 300 BglI GCCn_nnn'nGGC Cuts at: 0 19 98 263 1680 Size: 19 79 165 1417 Fragments arranged by size: 1417 165 79 19 BlpI GC'TnA_GC Cuts at: 0 402 1032 1680 Size: 402 630 648 Fragments arranged by size: 648 630 402 Bme1580I G_kGCm'C Cuts at: 0 646 732 1573 1680 Size: 646 86 841 107 Fragments arranged by size: 841 646 107 86 BmgBI CAC'GTC Cuts at: 0 36 1590 1680 Size: 36 1554 90 Fragments arranged by size: 1554 90 36 BmrI ACTGGGnnnn_n' Cuts at: 0 487 1362 1680 Size: 487 875 318 Fragments arranged by size: 875 487 318 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 591 1680 Size: 591 1089 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 179 195 862 1011 1087 1483 1680 Size: 179 16 667 149 76 396 197 Fragments arranged by size: 667 396 197 179 149 76 16 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 129 1680 Size: 129 1551 BsaI GGTCTCn'nnnn_ Cuts at: 0 213 336 361 1474 1663 1680 Size: 213 123 25 1113 189 17 Fragments arranged by size: 1113 213 189 123 25 17 BsaBI GATnn'nnATC Cuts at: 0 190 547 1680 Size: 190 357 1133 Fragments arranged by size: 1133 357 190 BsaHI Gr'CG_yC Cuts at: 0 120 266 1680 Size: 120 146 1414 Fragments arranged by size: 1414 146 120 BsaWI w'CCGG_w Cuts at: 0 223 428 1680 Size: 223 205 1252 Fragments arranged by size: 1252 223 205 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 46 85 524 538 580 1014 1630 1680 Size: 46 39 439 14 42 434 616 50 Fragments arranged by size: 616 439 434 50 46 42 39 14 BseYI C'CCAG_C Cuts at: 0 441 666 705 876 1389 1517 1680 Size: 441 225 39 171 513 128 163 Fragments arranged by size: 513 441 225 171 163 128 39 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 60 604 1629 1680 Size: 60 544 1025 51 Fragments arranged by size: 1025 544 60 51 BsiEI CG_ry'CG Cuts at: 0 1086 1680 Size: 1086 594 BsiHKAI G_wGCw'C Cuts at: 0 167 403 917 1301 1562 1680 Size: 167 236 514 384 261 118 Fragments arranged by size: 514 384 261 236 167 118 Bsp1286I G_dGCh'C Cuts at: 0 167 403 646 732 917 926 1301 1359 Size: 167 236 243 86 185 9 375 58 Cuts at: 1359 1562 1573 1680 Size: 203 11 107 Fragments arranged by size: 375 243 236 203 185 167 107 86 58 11 9 BspEI T'CCGG_A Cuts at: 0 223 428 1680 Size: 223 205 1252 Fragments arranged by size: 1252 223 205 BspHI T'CATG_A Cuts at: 0 395 770 1680 Size: 395 375 910 Fragments arranged by size: 910 395 375 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1585 1680 Size: 1585 95 BsrBI CCG'CTC Cuts at: 0 1608 1680 Size: 1608 72 BsrDI GCAATG_nn' Cuts at: 0 824 1340 1680 Size: 824 516 340 Fragments arranged by size: 824 516 340 BsrFI r'CCGG_y Cuts at: 0 1085 1273 1680 Size: 1085 188 407 Fragments arranged by size: 1085 407 188 BstAPI GCAn_nnn'nTGC Cuts at: 0 1002 1680 Size: 1002 678 BstXI CCAn_nnnn'nTGG Cuts at: 0 157 303 1131 1525 1680 Size: 157 146 828 394 155 Fragments arranged by size: 828 394 157 155 146 BstYI r'GATC_y Cuts at: 0 542 836 1680 Size: 542 294 844 Fragments arranged by size: 844 542 294 BtgI C'CryG_G Cuts at: 0 275 371 692 896 918 984 1112 1286 Size: 275 96 321 204 22 66 128 174 Cuts at: 1286 1307 1680 Size: 21 373 Fragments arranged by size: 373 321 275 204 174 128 96 66 22 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 665 993 1077 1158 1680 Size: 665 328 84 81 522 Fragments arranged by size: 665 522 328 84 81 BtsI GCAGTG_nn' Cuts at: 0 1039 1139 1235 1641 1680 Size: 1039 100 96 406 39 Fragments arranged by size: 1039 406 100 96 39 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 477 512 1680 Size: 477 35 1168 Fragments arranged by size: 1168 477 35 EaeI y'GGCC_r Cuts at: 0 1016 1160 1206 1680 Size: 1016 144 46 474 Fragments arranged by size: 1016 474 144 46 EarI CTCTTCn'nnn_ Cuts at: 0 1531 1680 Size: 1531 149 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 60 555 1680 Size: 60 495 1125 Fragments arranged by size: 1125 495 60 EcoICRI GAG'CTC Cuts at: 0 165 401 1560 1680 Size: 165 236 1159 120 Fragments arranged by size: 1159 236 165 120 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 179 195 202 862 1011 1087 1483 1680 Size: 179 16 7 660 149 76 396 197 Fragments arranged by size: 660 396 197 179 149 76 16 7 EcoO109I rG'GnC_Cy Cuts at: 0 113 528 728 729 1570 1680 Size: 113 415 200 1 841 110 Fragments arranged by size: 841 415 200 113 110 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 897 1680 Size: 897 783 FseI GG_CCGG'CC Cuts at: 0 1277 1680 Size: 1277 403 HaeII r_GCGC'y Cuts at: 0 123 1680 Size: 123 1557 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 44 76 1680 Size: 44 32 1604 Fragments arranged by size: 1604 44 32 HincII GTy'rAC Cuts at: 0 1185 1350 1680 Size: 1185 165 330 Fragments arranged by size: 1185 330 165 KasI G'GCGC_C Cuts at: 0 119 1680 Size: 119 1561 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 398 1411 1680 Size: 398 1013 269 Fragments arranged by size: 1013 398 269 MscI TGG'CCA Cuts at: 0 1018 1208 1680 Size: 1018 190 472 Fragments arranged by size: 1018 472 190 MslI CAynn'nnrTG Cuts at: 0 301 325 661 898 1129 1306 1680 Size: 301 24 336 237 231 177 374 Fragments arranged by size: 374 336 301 237 231 177 24 MspA1I CmG'CkG Cuts at: 0 725 986 1258 1408 1627 1680 Size: 725 261 272 150 219 53 Fragments arranged by size: 725 272 261 219 150 53 NaeI GCC'GGC Cuts at: 0 1275 1680 Size: 1275 405 NarI GG'CG_CC Cuts at: 0 120 1680 Size: 120 1560 NcoI C'CATG_G Cuts at: 0 692 1286 1680 Size: 692 594 394 Fragments arranged by size: 692 594 394 NgoMIV G'CCGG_C Cuts at: 0 1273 1680 Size: 1273 407 NspI r_CATG'y Cuts at: 0 13 438 1680 Size: 13 425 1242 Fragments arranged by size: 1242 425 13 PasI CC'CwG_GG Cuts at: 0 857 1566 1680 Size: 857 709 114 Fragments arranged by size: 857 709 114 PciI A'CATG_T Cuts at: 0 434 1680 Size: 434 1246 PfoI T'CCnGG_A Cuts at: 0 560 839 1499 1680 Size: 560 279 660 181 Fragments arranged by size: 660 560 279 181 PpuMI rG'GwC_Cy Cuts at: 0 113 1680 Size: 113 1567 PshAI GACnn'nnGTC Cuts at: 0 1675 1680 Size: 1675 5 PspOMI G'GGCC_C Cuts at: 0 728 1569 1680 Size: 728 841 111 Fragments arranged by size: 841 728 111 PstI C_TGCA'G Cuts at: 0 998 1242 1680 Size: 998 244 438 Fragments arranged by size: 998 438 244 PvuII CAG'CTG Cuts at: 0 1258 1680 Size: 1258 422 SacI G_AGCT'C Cuts at: 0 167 403 1562 1680 Size: 167 236 1159 118 Fragments arranged by size: 1159 236 167 118 SacII CC_GC'GG Cuts at: 0 987 1680 Size: 987 693 SbfI CC_TGCA'GG Cuts at: 0 998 1680 Size: 998 682 SfcI C'TryA_G Cuts at: 0 994 1238 1680 Size: 994 244 442 Fragments arranged by size: 994 442 244 SfoI GGC'GCC Cuts at: 0 121 1680 Size: 121 1559 SmaI CCC'GGG Cuts at: 0 1447 1680 Size: 1447 233 SmlI C'TyrA_G Cuts at: 0 108 1680 Size: 108 1572 SphI G_CATG'C Cuts at: 0 13 1680 Size: 13 1667 StuI AGG'CCT Cuts at: 0 1552 1680 Size: 1552 128 StyI C'CwwG_G Cuts at: 0 509 692 1286 1680 Size: 509 183 594 394 Fragments arranged by size: 594 509 394 183 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 359 706 756 1105 1381 1680 Size: 359 347 50 349 276 299 Fragments arranged by size: 359 349 347 299 276 50 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 568 1128 1680 Size: 568 560 552 Fragments arranged by size: 568 560 552 XmaI C'CCGG_G Cuts at: 0 1445 1680 Size: 1445 235 XmnI GAAnn'nnTTC Cuts at: 0 436 1680 Size: 436 1244 Enzymes that do cut: AarI AcuI AflIII AleI AlfI AlwNI ApaI AvaI BamHI BanI BanII BbeI BbsI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BstAPI BstXI BstYI BtgI BtgZI BtsI CspCI EaeI EarI EciI EcoICRI Eco57MI EcoO109I FalI FseI HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PfoI PpuMI PshAI PspOMI PstI PvuII SacI SacII SbfI SfcI SfoI SmaI SmlI SphI StuI StyI TaqII XcmI XmaI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbvCI BcgI BciVI BclI BfrBI BglII BmtI Bpu10I BsaAI BsaXI BsiWI BsmI BsmBI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I ClaI DraI DraIII DrdI EagI EcoNI EcoRI EcoRV FspI FspAI HindIII HpaI KpnI MfeI MluI NdeI NheI NotI NruI NsiI PacI PflMI PmeI PmlI PpiI PsiI PspXI PsrI PvuI RsrII SalI SanDI SapI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI TatI TstI Tth111I XbaI XhoI ZraI