################################## # NO CUT ENZYMES ( 81 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AgeI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AviII BbrPI BclI BsaBI BsaWI BseAI BsiWI BspDI BspEI BspHI BspLU11I BssHII BstBI BstEII BstPI ClaI DraI DraIII DrdI Eco47III EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI MamI MfeI MroI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI SacI SalI ScaI SfiI SfuI SgrAI SnaBI SpeI SspI SwaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AccI 1098 GTATAC GT^[AC][GT]AC AflII 553 CTTAAG C^TTAAG AocI 277 CCTGAGG CC^T[ACGT]AGG AspHI 1144 GTGCTC G[AT]GC[AT]^C AvrII 1416 CCTAGG C^CTAGG BbsI 2341 GTCTTC GAAGAC(2/6) BbvCI 1713 CCTCAGC CCTCAGC(-5/-2) BfrI 553 CTTAAG C^TTAAG BlnI 1416 CCTAGG C^CTAGG BlpI 178 GCTGAGC GC^T[ACGT]AGC Bpu1102I 178 GCTGAGC GC^T[ACGT]AGC BpuAI 2341 GTCTTC GAAGAC(2/6) BsiHKAI 1144 GTGCTC G[AT]GC[AT]^C BsmI 596 GCATTC GAATGC(1/-1) BsrDI 1847 GCAATG GCAATG(2/0) Bst1107I 1098 GTATAC GTA^TAC BstZ17I 1098 GTATAC GTA^TAC Bsu36I 277 CCTGAGG CC^T[ACGT]AGG CelII 178 GCTGAGC GC^T[ACGT]AGC CpoI 650 CGGACCG CG^G[AT]CCG EarI 1269 CTCTTC CTCTTC(1/4) Eco81I 277 CCTGAGG CC^T[ACGT]AGG Ksp632I 1269 CTCTTC CTCTTC(1/4) MluI 1118 ACGCGT A^CGCGT MseI 554 TTAA T^TAA RsrII 650 CGGACCG CG^G[AT]CCG SapI 1268 GCTCTTC GCTCTTC(1/4) SbfI 750 CCTGCAGG CCTGCA^GG SmaI 1388 CCCGGG CCC^GGG Sse8387I 750 CCTGCAGG CCTGCA^GG Tru9I 554 TTAA T^TAA Tsp509I 1009 AATT ^AATT XbaI 1353 TCTAGA T^CTAGA XcmI 776 CCACAGCCGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 1388 CCCGGG C^CCGGG XmaI 1388 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 39 enzymes) # ################################### AhdI 385 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2011 GACGTGTGGTC ApaI 1630 GGGCCC GGGCC^C 1688 GGGCCC AspEI 385 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2011 GACGTGTGGTC AspI 1546 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 2230 GACAAGGTC BalI 189 TGGCCA TGG^CCA 1434 TGGCCA BamHI 2027 GGATCC G^GATCC 2368 GGATCC BbeI 1694 GGCGCC GGCGC^C 1968 GGCGCC BcgI 872 GCAGCAGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1007 GCAATTCCATCG BciVI 1823 GTATCC GTATCC(6/5) 2067 GTATCC BglI 998 GCCTCGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1697 GCCCCCCAGGC BglII 1356 AGATCT A^GATCT 2042 AGATCT BsaAI 1445 TACGTG [CT]AC^GT[GA] 1639 TACGTG BsaI 1551 GGTCTC GGTCTC(1/5) 1767 GGTCTC BsmBI 1309 CGTCTC CGTCTC(1/5) 2247 CGTCTC Bsp1407I 818 TGTACA T^GTACA 1994 TGTACA BsrBI 1747 CCGCTC CCGCTC(-3/-3) 727 GAGCGG BsrGI 818 TGTACA T^GTACA 1994 TGTACA BstXI 776 CCACAGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1490 CCAGCACTGTGG Eam1105I 385 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2011 GACGTGTGGTC KasI 1694 GGCGCC G^GCGCC 1968 GGCGCC KspI 654 CCGCGG CCGC^GG 1902 CCGCGG MluNI 189 TGGCCA TGG^CCA 1434 TGGCCA MscI 189 TGGCCA TGG^CCA 1434 TGGCCA NaeI 1190 GCCGGC GCC^GGC 1339 GCCGGC NarI 1694 GGCGCC GG^CGCC 1968 GGCGCC NgoMIV 1190 GCCGGC G^CCGGC 1339 GCCGGC NspI 477 GCATGC [GA]CATG^[CT] 1796 GCATGC PflFI 1546 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 2230 GACAAGGTC PflMI 456 CCAGCGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 542 CCATCCGCTGG PvuII 518 CAGCTG CAG^CTG 2212 CAGCTG SacII 654 CCGCGG CCGC^GG 1902 CCGCGG SexAI 1547 ACCTGGT A^CC[AT]GGT 1781 ACCAGGT SfoI 1694 GGCGCC GGC^GCC 1968 GGCGCC SmlI 88 CTTGAG C^T[CT][GA]AG 553 CTTAAG SphI 477 GCATGC GCATG^C 1796 GCATGC SspBI 818 TGTACA T^GTACA 1994 TGTACA TfiI 333 GAATC G^A[AT]TC 2335 GATTC Tth111I 1546 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 2230 GACAAGGTC Van91I 456 CCAGCGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 542 CCATCCGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 102 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AluI AlwI AlwNI AvaI AvaII BanI BanII BbvI BcnI BfaI BmyI BpmI BsaHI BsaJI BseRI BsgI BsiEI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EclXI Eco52I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StuI StyI TaqI TseI Tsp45I TspRI TthHB8I XhoII