(Circular) (Six-base) MAPSORT of: pFN21AE1340.seq Check: 6036 from: 1 to: 7111 pFN21AE1340 7111 bp With 182 enzymes: SgfI * August 16, 2010 14:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2236 2681 3008 4334 2236 Size: 445 327 1326 5013 Fragments arranged by size: 5013 1326 445 327 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4921 278 Size: 53 83 186 936 3385 2468 Fragments arranged by size: 3385 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3104 4454 1937 Size: 1167 1350 4594 Fragments arranged by size: 4594 1350 1167 Acc65I G'GTAC_C Cuts at: 4432 4432 Size: 7111 AclI AA'CG_TT Cuts at: 1563 5238 5611 1563 Size: 3675 373 3063 Fragments arranged by size: 3675 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2876 4333 5174 6558 6826 1632 Size: 1244 1457 841 1384 268 1917 Fragments arranged by size: 1917 1457 1384 1244 841 268 AflII C'TTAA_G Cuts at: 820 1017 2558 820 Size: 197 1541 5373 Fragments arranged by size: 5373 1541 197 AflIII A'CryG_T Cuts at: 1203 2970 3123 4017 6010 6987 1203 Size: 1767 153 894 1993 977 1327 Fragments arranged by size: 1993 1767 1327 977 894 153 AgeI A'CCGG_T Cuts at: 5942 5942 Size: 7111 AhdI GACnn_n'nnGTC Cuts at: 2395 4021 5840 2395 Size: 1626 1819 3666 Fragments arranged by size: 3666 1819 1626 AleI CACnn'nnGTG Cuts at: 1166 2786 4011 1166 Size: 1620 1225 4266 Fragments arranged by size: 4266 1620 1225 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2624 1184 Size: 1440 5671 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4082 4114 4082 Size: 32 7079 AlwNI CAG_nnn'CTG Cuts at: 2137 2492 2854 3811 4326 4854 6426 2137 Size: 355 362 957 515 528 1572 2822 Fragments arranged by size: 2822 1572 957 528 515 362 355 ApaI G_GGCC'C Cuts at: 3639 3697 3639 Size: 58 7053 ApaLI G'TGCA_C Cuts at: 5168 6324 5168 Size: 1156 5955 ApoI r'AATT_y Cuts at: 1493 4420 4646 4798 6907 1493 Size: 2927 226 152 2109 1697 Fragments arranged by size: 2927 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5665 160 Size: 872 4633 1606 Fragments arranged by size: 4633 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7111 AvaI C'yCGr_G Cuts at: 1870 1943 3092 3393 3795 3977 4058 4436 1870 Size: 73 1149 301 402 182 81 378 4545 Fragments arranged by size: 4545 1149 402 378 301 182 81 73 AvrII C'CTAG_G Cuts at: 3421 3421 Size: 7111 BamHI G'GATC_C Cuts at: 4032 4373 4441 4808 4032 Size: 341 68 367 6335 Fragments arranged by size: 6335 367 341 68 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2224 2647 3198 3699 3973 Size: 325 217 627 437 423 551 501 274 Cuts at: 3973 4432 5887 618 Size: 459 1455 1842 Fragments arranged by size: 1842 1455 627 551 501 459 437 423 325 274 217 BanII G_rGCy'C Cuts at: 1391 2100 2769 3134 3639 3697 4067 4430 1391 Size: 709 669 365 505 58 370 363 4072 Fragments arranged by size: 4072 709 669 505 370 365 363 58 BbeI G_GCGC'C Cuts at: 3703 3977 3703 Size: 274 6837 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4339 928 Size: 971 2440 3700 Fragments arranged by size: 3700 2440 971 BbvCI CC'TCA_GC Cuts at: 3719 3719 Size: 7111 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2866 2900 3001 3035 5302 Size: 34 50 34 845 34 101 34 2267 Cuts at: 5302 5336 1903 Size: 34 3678 Fragments arranged by size: 3678 2267 845 101 50 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3839 4083 5003 6213 3839 Size: 244 920 1210 4737 Fragments arranged by size: 4737 1210 920 244 BclI T'GATC_A Cuts at: 1548 1548 Size: 7111 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3009 3708 5722 Size: 107 122 71 631 605 1337 699 2014 Cuts at: 5722 136 Size: 1525 Fragments arranged by size: 2014 1525 1337 699 631 605 122 107 71 BglII A'GATC_T Cuts at: 3361 4047 7106 3361 Size: 686 3059 3366 Fragments arranged by size: 3366 3059 686 BlpI GC'TnA_GC Cuts at: 2184 4528 2184 Size: 2344 4767 Bme1580I G_kGCm'C Cuts at: 1790 2652 3639 3697 5172 6328 1790 Size: 862 987 58 1475 1156 2573 Fragments arranged by size: 2573 1475 1156 987 862 58 BmgBI CAC'GTC Cuts at: 1318 2803 4018 1318 Size: 1485 1215 4411 Fragments arranged by size: 4411 1485 1215 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3758 4842 5800 Size: 416 53 83 399 24 2341 1084 958 Cuts at: 5800 442 Size: 1753 Fragments arranged by size: 2341 1753 1084 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7111 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3289 4117 3289 Size: 828 6283 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2040 2090 2138 2269 2356 2644 2750 3284 Size: 801 50 48 131 87 288 106 534 Cuts at: 3284 3307 3833 4318 5771 1239 Size: 23 526 485 1453 2579 Fragments arranged by size: 2579 1453 801 534 526 485 288 131 106 87 50 48 23 Bpu10I CC'TnA_GC Cuts at: 3719 3719 Size: 7111 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2114 5236 6101 6399 2114 Size: 3122 865 298 2826 Fragments arranged by size: 3122 2826 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3562 3778 5774 882 Size: 617 2063 216 1996 2219 Fragments arranged by size: 2219 2063 1996 617 216 BsaAI yAC'GTr Cuts at: 493 1204 3452 3646 4902 493 Size: 711 2248 194 1256 2702 Fragments arranged by size: 2702 2248 1256 711 194 BsaBI GATnn'nnATC Cuts at: 7105 7105 Size: 7111 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3700 3912 3974 4314 Size: 53 83 186 936 2167 212 62 340 Cuts at: 4314 4918 5300 5935 275 Size: 604 382 635 1451 Fragments arranged by size: 2167 1451 936 635 604 382 340 212 186 83 62 53 BsaWI w'CCGG_w Cuts at: 5544 5942 6216 6363 6774 5544 Size: 398 274 147 411 5881 Fragments arranged by size: 5881 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2798 2828 2942 2972 4082 Size: 30 165 30 1395 30 114 30 1110 Cuts at: 4082 4112 1178 Size: 30 4177 Fragments arranged by size: 4177 1395 1110 165 114 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3077 3310 4106 4274 4321 1186 Size: 1891 233 796 168 47 3976 Fragments arranged by size: 3976 1891 796 233 168 47 BseYI C'CCAG_C Cuts at: 2111 2433 2460 2835 6314 6810 2111 Size: 322 27 375 3479 496 2412 Fragments arranged by size: 3479 2412 496 375 322 27 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3242 3474 3607 4424 1163 Size: 573 1506 232 133 817 3850 Fragments arranged by size: 3850 1506 817 573 232 133 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3196 3589 3952 4561 5322 6350 Size: 275 186 1195 393 363 609 761 1028 Cuts at: 6350 1540 Size: 2301 Fragments arranged by size: 2301 1195 1028 761 609 393 363 275 186 BsiHKAI G_wGCw'C Cuts at: 3153 5172 5257 6328 3153 Size: 2019 85 1071 3936 Fragments arranged by size: 3936 2019 1071 85 BsmI GAATG_Cn' Cuts at: 2601 4622 4715 2601 Size: 2021 93 4997 Fragments arranged by size: 4997 2021 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3320 4258 3320 Size: 938 6173 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2100 2652 2769 3134 3153 3639 3697 Size: 399 310 552 117 365 19 486 58 Cuts at: 3697 4067 4430 5172 5257 6328 1391 Size: 370 363 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 552 486 399 370 365 363 310 117 85 58 19 BspEI T'CCGG_A Cuts at: 6774 6774 Size: 7111 BspHI T'CATG_A Cuts at: 5000 6730 5000 Size: 1730 5381 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2236 2500 2681 2724 2970 3008 3618 3684 Size: 1392 264 181 43 246 38 610 66 Cuts at: 3684 4334 4466 844 Size: 650 132 3489 Fragments arranged by size: 3489 1392 650 610 264 246 181 132 66 43 38 BsrBI CCG'CTC Cuts at: 1394 1823 2734 3754 4998 1394 Size: 429 911 1020 1244 3507 Fragments arranged by size: 3507 1244 1020 911 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3859 5606 5780 65 Size: 1183 2611 1747 174 1396 Fragments arranged by size: 2611 1747 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2745 3195 3344 5755 5942 1700 Size: 110 935 450 149 2411 187 2869 Fragments arranged by size: 2869 2411 935 450 187 149 110 BsrGI T'GTAC_A Cuts at: 96 2823 3999 96 Size: 2727 1176 3208 Fragments arranged by size: 3208 2727 1176 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7111 BssSI C'ACGA_G Cuts at: 2313 2630 3350 5171 6183 2313 Size: 317 720 1821 1012 3241 Fragments arranged by size: 3241 1821 1012 720 317 BstAPI GCAn_nnn'nTGC Cuts at: 794 2161 2492 4326 794 Size: 1367 331 1834 3579 Fragments arranged by size: 3579 1834 1367 331 BstBI TT'CG_AA Cuts at: 5915 5915 Size: 7111 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7111 BstXI CCAn_nnnn'nTGG Cuts at: 2788 3502 2788 Size: 714 6397 BstYI r'GATC_y Cuts at: 1972 3361 4032 4047 4373 4441 4808 5193 5210 Size: 1389 671 15 326 68 367 385 17 Cuts at: 5210 6662 6748 6760 7106 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1389 671 385 367 346 326 86 68 17 15 12 BstZ17I GTA'TAC Cuts at: 3105 3105 Size: 7111 Bsu36I CC'TnA_GG Cuts at: 2283 2283 Size: 7111 BtgI C'CryG_G Cuts at: 513 1065 2659 2787 3190 3907 513 Size: 552 1594 128 403 717 3717 Fragments arranged by size: 3717 1594 717 552 403 128 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2844 487 Size: 683 56 1618 4754 Fragments arranged by size: 4754 1618 683 56 BtsI GCAGTG_nn' Cuts at: 3748 4141 4627 5421 5441 3748 Size: 393 486 794 20 5418 Fragments arranged by size: 5418 794 486 393 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7111 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7076 DraI TTT'AAA Cuts at: 4415 4415 Size: 7111 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6118 809 Size: 507 4802 1802 Fragments arranged by size: 4802 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2194 2233 2293 Size: 54 1425 50 275 18 364 39 60 Cuts at: 2293 2772 3162 3193 3439 3504 3546 3586 3949 Size: 479 390 31 246 65 42 40 363 Cuts at: 3949 4248 4558 5447 7076 8 Size: 299 310 889 1629 43 Fragments arranged by size: 1629 1425 889 479 390 364 363 310 299 275 246 65 60 54 50 43 42 40 39 31 18 EagI C'GGCC_G Cuts at: 1537 1812 3193 3586 3949 4558 1537 Size: 275 1381 393 363 609 4090 Fragments arranged by size: 4090 1381 609 393 363 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3280 5041 1353 Size: 1927 1761 3423 Fragments arranged by size: 3423 1927 1761 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4265 5698 6072 6218 387 Size: 1064 138 2676 1433 374 146 1280 Fragments arranged by size: 2676 1433 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2040 2090 2138 2269 2356 2644 2750 Size: 393 408 50 48 131 87 288 106 Cuts at: 2750 2876 3284 3307 3833 4318 4333 5174 5771 Size: 126 408 23 526 485 15 841 597 Cuts at: 5771 6558 6826 1239 Size: 787 268 1524 Fragments arranged by size: 1524 841 787 597 526 485 408 408 393 288 268 131 126 106 87 50 48 23 15 EcoNI CCTnn'n_nnAGG Cuts at: 2282 2416 3425 4198 2282 Size: 134 1009 773 5195 Fragments arranged by size: 5195 1009 773 134 EcoO109I rG'GnC_Cy Cuts at: 2032 2389 2407 2953 3067 3541 3635 3636 3710 Size: 357 18 546 114 474 94 1 74 Cuts at: 3710 3838 4240 2032 Size: 128 402 4903 Fragments arranged by size: 4903 546 474 402 357 128 114 94 74 18 1 EcoRI G'AATT_C Cuts at: 4420 4798 4420 Size: 378 6733 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2108 2108 Size: 7111 FspI TGC'GCA Cuts at: 5617 5617 Size: 7111 HaeII r_GCGC'y Cuts at: 1678 3092 3188 3703 3977 4193 4232 6258 1678 Size: 1414 96 515 274 216 39 2026 2531 Fragments arranged by size: 2531 2026 1414 515 274 216 96 39 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2381 2413 3346 3378 3515 Size: 32 135 32 809 32 933 32 137 Cuts at: 3515 3542 3547 3574 4007 4039 5752 5784 5826 Size: 27 5 27 433 32 1713 32 42 Cuts at: 5826 5858 1373 Size: 32 2626 Fragments arranged by size: 2626 1713 933 809 433 137 135 42 32 32 32 32 32 32 32 27 27 5 HincII GTy'rAC Cuts at: 669 1657 1938 4455 4701 669 Size: 988 281 2517 246 3079 Fragments arranged by size: 3079 2517 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7111 HpaI GTT'AAC Cuts at: 4701 4701 Size: 7111 KasI G'GCGC_C Cuts at: 3699 3973 3699 Size: 274 6837 KpnI G_GTAC'C Cuts at: 4436 4436 Size: 7111 MfeI C'AATT_G Cuts at: 4710 4710 Size: 7111 MluI A'CGCG_T Cuts at: 3123 6987 3123 Size: 3864 3247 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6225 6409 1305 Size: 4920 184 2007 Fragments arranged by size: 4920 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2196 3441 10 Size: 54 1425 343 364 1245 3680 Fragments arranged by size: 3680 1425 1245 364 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2786 4011 4085 5069 5428 5587 518 Size: 648 1620 1225 74 984 359 159 2042 Fragments arranged by size: 2042 1620 1225 984 648 359 159 74 MspA1I CmG'CkG Cuts at: 1606 2517 2525 2553 2661 3327 3909 4219 4527 Size: 911 8 28 108 666 582 310 308 Cuts at: 4527 5204 6352 6597 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 911 677 666 582 310 308 245 108 28 8 NaeI GCC'GGC Cuts at: 3197 3346 3197 Size: 149 6962 NarI GG'CG_CC Cuts at: 3700 3974 3700 Size: 274 6837 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6559 NdeI CA'TA_TG Cuts at: 387 387 Size: 7111 NgoMIV G'CCGG_C Cuts at: 3195 3344 3195 Size: 149 6962 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7111 NotI GC'GGCC_GC Cuts at: 4558 4558 Size: 7111 NspI r_CATG'y Cuts at: 1132 1229 2486 3805 4469 6014 1132 Size: 97 1257 1319 664 1545 2229 Fragments arranged by size: 2229 1545 1319 1257 664 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7111 PciI A'CATG_T Cuts at: 6010 6010 Size: 7111 PflMI CCAn_nnn'nTGG Cuts at: 1108 2467 2553 1108 Size: 1359 86 5666 Fragments arranged by size: 5666 1359 86 PfoI T'CCnGG_A Cuts at: 3818 3818 Size: 7111 PmeI GTTT'AAAC Cuts at: 4415 4415 Size: 7111 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4082 4114 5180 5212 6693 6725 4082 Size: 32 1066 32 1481 32 4468 Fragments arranged by size: 4468 1481 1066 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2032 2389 2407 2953 3067 3541 3838 4240 2032 Size: 357 18 546 114 474 297 402 4903 Fragments arranged by size: 4903 546 474 402 357 297 114 18 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7111 PsiI TTA'TAA Cuts at: 4681 4681 Size: 7111 PspOMI G'GGCC_C Cuts at: 3635 3693 3635 Size: 58 7053 PstI C_TGCA'G Cuts at: 830 2337 2530 2760 2929 4463 830 Size: 1507 193 230 169 1534 3478 Fragments arranged by size: 3478 1534 1507 230 193 169 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7111 PvuII CAG'CTG Cuts at: 2525 4219 2525 Size: 1694 5417 RsrII CG'GwC_CG Cuts at: 2656 2656 Size: 7111 SacII CC_GC'GG Cuts at: 2662 3910 2662 Size: 1248 5863 SalI G'TCGA_C Cuts at: 1936 4453 1936 Size: 2517 4594 SanDI GG'GwC_CC Cuts at: 2407 2407 Size: 7111 SapI GCTCTTCn'nnn_ Cuts at: 3280 3280 Size: 7111 SbfI CC_TGCA'GG Cuts at: 2760 4463 2760 Size: 1703 5408 ScaI AGT'ACT Cuts at: 5359 7071 5359 Size: 1712 5399 SexAI A'CCwGG_T Cuts at: 3552 3786 3552 Size: 234 6877 SfcI C'TryA_G Cuts at: 826 1045 2333 2526 2756 2925 4459 5594 6275 Size: 219 1288 193 230 169 1534 1135 681 Cuts at: 6275 6466 826 Size: 191 1471 Fragments arranged by size: 1534 1471 1288 1135 681 230 219 193 191 169 SfoI GGC'GCC Cuts at: 3701 3975 3701 Size: 274 6837 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7111 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7111 SmaI CCC'GGG Cuts at: 1872 3395 4438 1872 Size: 1523 1043 4545 Fragments arranged by size: 4545 1523 1043 SmlI C'TyrA_G Cuts at: 820 1017 1943 2093 2558 5215 6116 6378 820 Size: 197 926 150 465 2657 901 262 1553 Fragments arranged by size: 2657 1553 926 901 465 262 197 150 SnaBI TAC'GTA Cuts at: 493 4902 493 Size: 4409 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7111 SphI G_CATG'C Cuts at: 1132 2486 3805 4469 1132 Size: 1354 1319 664 3774 Fragments arranged by size: 3774 1354 1319 664 SspI AAT'ATT Cuts at: 5 52 5035 5 Size: 47 4983 2081 Fragments arranged by size: 4983 2081 47 StuI AGG'CCT Cuts at: 1517 2075 2573 3461 3983 4204 1517 Size: 558 498 888 522 221 4424 Fragments arranged by size: 4424 888 558 522 498 221 StyI C'CwwG_G Cuts at: 513 1065 2149 3141 3421 3666 4550 513 Size: 552 1084 992 280 245 884 3074 Fragments arranged by size: 3074 1084 992 884 552 280 245 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3641 4146 5138 5155 5308 5493 5938 Size: 1255 2061 505 992 17 153 185 445 Cuts at: 5938 325 Size: 1498 Fragments arranged by size: 2061 1498 1255 992 505 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2823 2994 3999 Size: 274 80 33 51 1443 846 171 1005 Cuts at: 3999 5357 7069 96 Size: 1358 1712 138 Fragments arranged by size: 1712 1443 1358 1005 846 274 171 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2798 2830 7071 7103 2798 Size: 32 4241 32 2806 Fragments arranged by size: 4241 2806 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3554 4238 1316 Size: 2238 684 4189 Fragments arranged by size: 4189 2238 684 XbaI T'CTAG_A Cuts at: 3358 4447 3358 Size: 1089 6022 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2788 2788 Size: 7111 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7111 XmaI C'CCGG_G Cuts at: 1870 3393 4436 1870 Size: 1523 1043 4545 Fragments arranged by size: 4545 1523 1043 XmnI GAAnn'nnTTC Cuts at: 5240 5240 Size: 7111 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4919 276 Size: 53 83 186 936 3385 2468 Fragments arranged by size: 3385 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BaeI BfrBI BsiWI DraIII EcoICRI EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI PsrI SacI SfiI SrfI SwaI