(Linear) (Six-base) MAPSORT of: KIEE1340.seq Check: 8282 from: 1 to: 2406 KIEE1340.orf With 182 enzymes: SgfI * July 15, 2010 18:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 231 676 1003 2329 2406 Size: 231 445 327 1326 77 Fragments arranged by size: 1326 445 327 231 77 AccI GT'mk_AC Cuts at: 0 1099 2406 Size: 1099 1307 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 871 2328 2406 Size: 871 1457 78 Fragments arranged by size: 1457 871 78 AflII C'TTAA_G Cuts at: 0 553 2406 Size: 553 1853 AflIII A'CryG_T Cuts at: 0 965 1118 2012 2406 Size: 965 153 894 394 Fragments arranged by size: 965 894 394 153 AhdI GACnn_n'nnGTC Cuts at: 0 390 2016 2406 Size: 390 1626 390 Fragments arranged by size: 1626 390 390 AleI CACnn'nnGTG Cuts at: 0 781 2006 2406 Size: 781 1225 400 Fragments arranged by size: 1225 781 400 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 619 2406 Size: 619 1787 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2077 2109 2406 Size: 2077 32 297 Fragments arranged by size: 2077 297 32 AlwNI CAG_nnn'CTG Cuts at: 0 132 487 849 1806 2321 2406 Size: 132 355 362 957 515 85 Fragments arranged by size: 957 515 362 355 132 85 ApaI G_GGCC'C Cuts at: 0 1634 1692 2406 Size: 1634 58 714 Fragments arranged by size: 1634 714 58 AvaI C'yCGr_G Cuts at: 0 1087 1388 1790 1972 2053 2406 Size: 1087 301 402 182 81 353 Fragments arranged by size: 1087 402 353 301 182 81 AvrII C'CTAG_G Cuts at: 0 1416 2406 Size: 1416 990 BamHI G'GATC_C Cuts at: 0 2027 2368 2406 Size: 2027 341 38 Fragments arranged by size: 2027 341 38 BanI G'GyrC_C Cuts at: 0 219 642 1193 1694 1968 2406 Size: 219 423 551 501 274 438 Fragments arranged by size: 551 501 438 423 274 219 BanII G_rGCy'C Cuts at: 0 95 764 1129 1634 1692 2062 2406 Size: 95 669 365 505 58 370 344 Fragments arranged by size: 669 505 370 365 344 95 58 BbeI G_GCGC'C Cuts at: 0 1698 1972 2406 Size: 1698 274 434 Fragments arranged by size: 1698 434 274 BbsI GAAGACnn'nnnn_ Cuts at: 0 2334 2406 Size: 2334 72 BbvCI CC'TCA_GC Cuts at: 0 1714 2406 Size: 1714 692 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 861 895 996 1030 2406 Size: 861 34 101 34 1376 Fragments arranged by size: 1376 861 101 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1834 2078 2406 Size: 1834 244 328 Fragments arranged by size: 1834 328 244 BglI GCCn_nnn'nGGC Cuts at: 0 1004 1703 2406 Size: 1004 699 703 Fragments arranged by size: 1004 703 699 BglII A'GATC_T Cuts at: 0 1356 2042 2406 Size: 1356 686 364 Fragments arranged by size: 1356 686 364 BlpI GC'TnA_GC Cuts at: 0 179 2406 Size: 179 2227 Bme1580I G_kGCm'C Cuts at: 0 647 1634 1692 2406 Size: 647 987 58 714 Fragments arranged by size: 987 714 647 58 BmgBI CAC'GTC Cuts at: 0 798 2013 2406 Size: 798 1215 393 Fragments arranged by size: 1215 798 393 BmrI ACTGGGnnnn_n' Cuts at: 0 1753 2406 Size: 1753 653 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1284 2112 2406 Size: 1284 828 294 Fragments arranged by size: 1284 828 294 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 35 85 133 264 351 639 745 1279 Size: 35 50 48 131 87 288 106 534 Cuts at: 1279 1302 1828 2313 2406 Size: 23 526 485 93 Fragments arranged by size: 534 526 485 288 131 106 93 87 50 48 35 23 Bpu10I CC'TnA_GC Cuts at: 0 1714 2406 Size: 1714 692 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 109 2406 Size: 109 2297 BsaI GGTCTCn'nnnn_ Cuts at: 0 1557 1773 2406 Size: 1557 216 633 Fragments arranged by size: 1557 633 216 BsaAI yAC'GTr Cuts at: 0 1447 1641 2406 Size: 1447 194 765 Fragments arranged by size: 1447 765 194 BsaHI Gr'CG_yC Cuts at: 0 1695 1907 1969 2309 2406 Size: 1695 212 62 340 97 Fragments arranged by size: 1695 340 212 97 62 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 793 823 937 967 2077 2107 2406 Size: 793 30 114 30 1110 30 299 Fragments arranged by size: 1110 793 299 114 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1072 1305 2101 2269 2316 2406 Size: 1072 233 796 168 47 90 Fragments arranged by size: 1072 796 233 168 90 47 BseYI C'CCAG_C Cuts at: 0 106 428 455 830 2406 Size: 106 322 27 375 1576 Fragments arranged by size: 1576 375 322 106 27 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1237 1469 1602 2406 Size: 1237 232 133 804 Fragments arranged by size: 1237 804 232 133 BsiEI CG_ry'CG Cuts at: 0 1191 1584 1947 2406 Size: 1191 393 363 459 Fragments arranged by size: 1191 459 393 363 BsiHKAI G_wGCw'C Cuts at: 0 1148 2406 Size: 1148 1258 BsmI GAATG_Cn' Cuts at: 0 596 2406 Size: 596 1810 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1315 2253 2406 Size: 1315 938 153 Fragments arranged by size: 1315 938 153 Bsp1286I G_dGCh'C Cuts at: 0 95 647 764 1129 1148 1634 1692 2062 Size: 95 552 117 365 19 486 58 370 Cuts at: 2062 2406 Size: 344 Fragments arranged by size: 552 486 370 365 344 117 95 58 19 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 231 495 676 719 965 1003 1613 1679 Size: 231 264 181 43 246 38 610 66 Cuts at: 1679 2329 2406 Size: 650 77 Fragments arranged by size: 650 610 264 246 231 181 77 66 43 38 BsrBI CCG'CTC Cuts at: 0 729 1749 2406 Size: 729 1020 657 Fragments arranged by size: 1020 729 657 BsrDI GCAATG_nn' Cuts at: 0 1854 2406 Size: 1854 552 BsrFI r'CCGG_y Cuts at: 0 740 1190 1339 2406 Size: 740 450 149 1067 Fragments arranged by size: 1067 740 450 149 BsrGI T'GTAC_A Cuts at: 0 818 1994 2406 Size: 818 1176 412 Fragments arranged by size: 1176 818 412 BssSI C'ACGA_G Cuts at: 0 308 625 1345 2406 Size: 308 317 720 1061 Fragments arranged by size: 1061 720 317 308 BstAPI GCAn_nnn'nTGC Cuts at: 0 156 487 2321 2406 Size: 156 331 1834 85 Fragments arranged by size: 1834 331 156 85 BstXI CCAn_nnnn'nTGG Cuts at: 0 783 1497 2406 Size: 783 714 909 Fragments arranged by size: 909 783 714 BstYI r'GATC_y Cuts at: 0 1356 2027 2042 2368 2406 Size: 1356 671 15 326 38 Fragments arranged by size: 1356 671 326 38 15 BstZ17I GTA'TAC Cuts at: 0 1100 2406 Size: 1100 1306 Bsu36I CC'TnA_GG Cuts at: 0 278 2406 Size: 278 2128 BtgI C'CryG_G Cuts at: 0 654 782 1185 1902 2406 Size: 654 128 403 717 504 Fragments arranged by size: 717 654 504 403 128 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 839 2406 Size: 839 1567 BtsI GCAGTG_nn' Cuts at: 0 1743 2136 2406 Size: 1743 393 270 Fragments arranged by size: 1743 393 270 EaeI y'GGCC_r Cuts at: 0 189 228 288 767 1157 1188 1434 1499 Size: 189 39 60 479 390 31 246 65 Cuts at: 1499 1541 1581 1944 2243 2406 Size: 42 40 363 299 163 Fragments arranged by size: 479 390 363 299 246 189 163 65 60 42 40 39 31 EagI C'GGCC_G Cuts at: 0 1188 1581 1944 2406 Size: 1188 393 363 462 Fragments arranged by size: 1188 462 393 363 EarI CTCTTCn'nnn_ Cuts at: 0 1275 2406 Size: 1275 1131 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 2260 2406 Size: 2260 146 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 35 85 133 264 351 639 745 871 Size: 35 50 48 131 87 288 106 126 Cuts at: 871 1279 1302 1828 2313 2328 2406 Size: 408 23 526 485 15 78 Fragments arranged by size: 526 485 408 288 131 126 106 87 78 50 48 35 23 15 EcoNI CCTnn'n_nnAGG Cuts at: 0 277 411 1420 2193 2406 Size: 277 134 1009 773 213 Fragments arranged by size: 1009 773 277 213 134 EcoO109I rG'GnC_Cy Cuts at: 0 27 384 402 948 1062 1536 1630 1631 Size: 27 357 18 546 114 474 94 1 Cuts at: 1631 1705 1833 2235 2406 Size: 74 128 402 171 Fragments arranged by size: 546 474 402 357 171 128 114 94 74 27 18 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 103 2406 Size: 103 2303 HaeII r_GCGC'y Cuts at: 0 1087 1183 1698 1972 2188 2227 2406 Size: 1087 96 515 274 216 39 179 Fragments arranged by size: 1087 515 274 216 179 96 39 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 376 408 1341 1373 1510 1537 1542 1569 Size: 376 32 933 32 137 27 5 27 Cuts at: 1569 2002 2034 2406 Size: 433 32 372 Fragments arranged by size: 933 433 376 372 137 32 32 32 27 27 5 KasI G'GCGC_C Cuts at: 0 1694 1968 2406 Size: 1694 274 438 Fragments arranged by size: 1694 438 274 MluI A'CGCG_T Cuts at: 0 1118 2406 Size: 1118 1288 MscI TGG'CCA Cuts at: 0 191 1436 2406 Size: 191 1245 970 Fragments arranged by size: 1245 970 191 MslI CAynn'nnrTG Cuts at: 0 781 2006 2080 2406 Size: 781 1225 74 326 Fragments arranged by size: 1225 781 326 74 MspA1I CmG'CkG Cuts at: 0 512 520 548 656 1322 1904 2214 2406 Size: 512 8 28 108 666 582 310 192 Fragments arranged by size: 666 582 512 310 192 108 28 8 NaeI GCC'GGC Cuts at: 0 1192 1341 2406 Size: 1192 149 1065 Fragments arranged by size: 1192 1065 149 NarI GG'CG_CC Cuts at: 0 1695 1969 2406 Size: 1695 274 437 Fragments arranged by size: 1695 437 274 NgoMIV G'CCGG_C Cuts at: 0 1190 1339 2406 Size: 1190 149 1067 Fragments arranged by size: 1190 1067 149 NspI r_CATG'y Cuts at: 0 481 1800 2406 Size: 481 1319 606 Fragments arranged by size: 1319 606 481 PflMI CCAn_nnn'nTGG Cuts at: 0 462 548 2406 Size: 462 86 1858 Fragments arranged by size: 1858 462 86 PfoI T'CCnGG_A Cuts at: 0 1813 2406 Size: 1813 593 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2077 2109 2406 Size: 2077 32 297 Fragments arranged by size: 2077 297 32 PpuMI rG'GwC_Cy Cuts at: 0 27 384 402 948 1062 1536 1833 2235 Size: 27 357 18 546 114 474 297 402 Cuts at: 2235 2406 Size: 171 Fragments arranged by size: 546 474 402 357 297 171 114 27 18 PspOMI G'GGCC_C Cuts at: 0 1630 1688 2406 Size: 1630 58 718 Fragments arranged by size: 1630 718 58 PstI C_TGCA'G Cuts at: 0 332 525 755 924 2406 Size: 332 193 230 169 1482 Fragments arranged by size: 1482 332 230 193 169 PvuII CAG'CTG Cuts at: 0 520 2214 2406 Size: 520 1694 192 Fragments arranged by size: 1694 520 192 RsrII CG'GwC_CG Cuts at: 0 651 2406 Size: 651 1755 SacII CC_GC'GG Cuts at: 0 657 1905 2406 Size: 657 1248 501 Fragments arranged by size: 1248 657 501 SanDI GG'GwC_CC Cuts at: 0 402 2406 Size: 402 2004 SapI GCTCTTCn'nnn_ Cuts at: 0 1275 2406 Size: 1275 1131 SbfI CC_TGCA'GG Cuts at: 0 755 2406 Size: 755 1651 SexAI A'CCwGG_T Cuts at: 0 1547 1781 2406 Size: 1547 234 625 Fragments arranged by size: 1547 625 234 SfcI C'TryA_G Cuts at: 0 328 521 751 920 2406 Size: 328 193 230 169 1486 Fragments arranged by size: 1486 328 230 193 169 SfoI GGC'GCC Cuts at: 0 1696 1970 2406 Size: 1696 274 436 Fragments arranged by size: 1696 436 274 SmaI CCC'GGG Cuts at: 0 1390 2406 Size: 1390 1016 SmlI C'TyrA_G Cuts at: 0 88 553 2406 Size: 88 465 1853 Fragments arranged by size: 1853 465 88 SphI G_CATG'C Cuts at: 0 481 1800 2406 Size: 481 1319 606 Fragments arranged by size: 1319 606 481 StuI AGG'CCT Cuts at: 0 70 568 1456 1978 2199 2406 Size: 70 498 888 522 221 207 Fragments arranged by size: 888 522 498 221 207 70 StyI C'CwwG_G Cuts at: 0 144 1136 1416 1661 2406 Size: 144 992 280 245 745 Fragments arranged by size: 992 745 280 245 144 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1636 2141 2406 Size: 1636 505 265 Fragments arranged by size: 1636 505 265 TatI w'GTAC_w Cuts at: 0 818 989 1994 2406 Size: 818 171 1005 412 Fragments arranged by size: 1005 818 412 171 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 793 825 2406 Size: 793 32 1581 Fragments arranged by size: 1581 793 32 Tth111I GACn'n_nGTC Cuts at: 0 1549 2233 2406 Size: 1549 684 173 Fragments arranged by size: 1549 684 173 XbaI T'CTAG_A Cuts at: 0 1353 2406 Size: 1353 1053 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 783 2406 Size: 783 1623 XmaI C'CCGG_G Cuts at: 0 1388 2406 Size: 1388 1018 Enzymes that do cut: AarI AccI AcuI AflII AflIII AhdI AleI AlfI AloI AlwNI ApaI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I KasI MluI MscI MslI MspA1I NaeI NarI NgoMIV NspI PflMI PfoI PpiI PpuMI PspOMI PstI PvuII RsrII SacII SanDI SapI SbfI SexAI SfcI SfoI SmaI SmlI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AatII Acc65I AclI AfeI AgeI ApaLI ApoI AscI AseI AsiSI BaeI BclI BfrBI BmtI BsaBI BsaWI BsiWI BspEI BspHI BssHII BstBI BstEII ClaI CspCI DraI DraIII DrdI EcoICRI EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MmeI NcoI NdeI NheI NotI NruI NsiI PacI PasI PciI PmeI PmlI PshAI PsiI PspXI PsrI PvuI SacI SalI ScaI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI XhoI XmnI ZraI