Restriction map for
pFN21AB0412
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# NO CUT ENZYMES ( 95 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AgeI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspI AviII AvrII BalI BbeI
BbrPI BbvCI BciVI BclI BglI
BglII BlnI BsaAI BsaHI BsaI
BsaWI BseAI BsiWI BsmBI BspDI
BspEI BspHI BspLU11I BssHII BstAPI
BstBI Bsu36I ClaI DraIII DrdI
Eco47III Eco81I EcoNI EcoT14I FbaI
FseI FspI Hin1I HpaI KasI
KpnI MfeI MluI MluNI MroI
MscI MunI NarI NcoI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
SacI SalI SbfI SfiI SfoI
SfuI SnaBI SpeI SphI Sse8387I
StuI StyI SwaI Tth111I XhoI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 611 GTATAC GT^[AC][GT]AC
AflII 384 CTTAAG C^TTAAG
AflIII 1760 ACACGT A^C[GA][CT]GT
AspHI 962 GAGCAC G[AT]GC[AT]^C
BamHI 113 GGATCC G^GATCC
BanI 325 GGCACC G^G[CT][GA]CC
BcgI 44 CGACTGCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 384 CTTAAG C^TTAAG
BseRI 2320 GAGGAG GAGGAG(10/8)
BsiHKAI 962 GAGCAC G[AT]GC[AT]^C
BsmI 2391 GAATGC GAATGC(1/-1)
Bsp1407I 429 TGTACA T^GTACA
BspMI 75 GCAGGT ACCTGC(4/8)
BsrBI 125 CCGCTC CCGCTC(-3/-3)
BsrDI 1075 CATTGC GCAATG(2/0)
BsrGI 429 TGTACA T^GTACA
BssSI 1109 CTCGTG CACGAG(-5/-1)
Bst1107I 611 GTATAC GTA^TAC
BstEII 1468 GGTGACC G^GT[ACGT]ACC
BstPI 1468 GGTGACC G^GT[ACGT]ACC
BstZ17I 611 GTATAC GTA^TAC
CfoI 288 GCGC GCG^C
CpoI 121 CGGACCG CG^G[AT]CCG
DraI 1741 TTTAAA TTT^AAA
DsaI 145 CCGCGG C^C[GA][CT]GG
EcoO65I 1468 GGTGACC G^GT[ACGT]ACC
EcoRV 979 GATATC GAT^ATC
HaeII 287 GGCGCT [GA]GCGC^[CT]
HgaI 1041 GCGTC GACGC(5/10)
HhaI 288 GCGC GCG^C
HinP1I 288 GCGC G^CGC
HincII 1764 GTTGAC GT[CT]^[GA]AC
HindII 1764 GTTGAC GT[CT]^[GA]AC
KspI 145 CCGCGG CCGC^GG
MslI 1378 CATACATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI 1382 CATATG CA^TATG
NheI 2179 GCTAGC G^CTAGC
PflMI 2095 CCACCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 2041 CAGCTG CAG^CTG
RsrII 121 CGGACCG CG^G[AT]CCG
SacII 145 CCGCGG CCGC^GG
ScaI 2324 AGTACT AGT^ACT
SexAI 1685 ACCAGGT A^CC[AT]GGT
SfcI 2340 CTACAG C^T[GA][CT]AG
SgrAI 257 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 100 CCCGGG CCC^GGG
SspBI 429 TGTACA T^GTACA
SspI 2357 AATATT AAT^ATT
Van91I 2095 CCACCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1486 TCTAGA T^CTAGA
XmaCI 100 CCCGGG C^CCGGG
XmaI 100 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AccII 146 CGCG CG^CG
209 CGCG
AhdI 187 GACTTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1508 GACTATCAGTC
Asp700I 1490 GAAATAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1985 GAACCTCTTC
AspEI 187 GACTTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1508 GACTATCAGTC
AvaI 21 CTCGGG C^[CT]CG[GA]G
100 CCCGGG
BanII 295 GGGCTC G[GA]GC[CT]^C
2246 GAGCCC
BfaI 1487 CTAG C^TAG
2180 CTAG
BlpI 58 GCTGAGC GC^T[ACGT]AGC
2191 GCTAAGC
Bpu1102I 58 GCTGAGC GC^T[ACGT]AGC
2191 GCTAAGC
BsaBI 396 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2302 GATACCTATC
BsgI 73 GTGCAG GTGCAG(16/14)
262 GTGCAG
BsoBI 21 CTCGGG C^[CT]CG[GA]G
100 CCCGGG
BstUI 146 CGCG CG^CG
209 CGCG
CelII 58 GCTGAGC GC^T[ACGT]AGC
2191 GCTAAGC
Eam1105I 187 GACTTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1508 GACTATCAGTC
EcoT22I 1180 ATGCAT ATGCA^T
2458 ATGCAT
MaeI 1487 CTAG C^TAG
2180 CTAG
MamI 396 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2302 GATACCTATC
MvnI 146 CGCG CG^CG
209 CGCG
NsiI 1180 ATGCAT ATGCA^T
2458 ATGCAT
NspI 63 GCATGT [GA]CATG^[CT]
1178 ACATGC
PpuMI 77 AGGTCCC [GA]G^G[AT]CC[CT]
502 GGGACCC
SapI 1627 GAAGAGC GCTCTTC(1/4)
2027 GCTCTTC
XcmI 350 CCACCATAGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1560 CCACTCAGAATGTGG
XmnI 1490 GAAATAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1985 GAACCTCTTC
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# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaII BbsI BbvI
BcnI BmyI BpmI BpuAI BsaJI
BsiEI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrFI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I Cfr10I
Cfr13I DdeI DpnII DraII EaeI
EagI EarI EclXI Eco52I EcoO109I
EcoRI EcoRII FauI Fnu4HI FokI
HaeIII HapII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII