################################## # NO CUT ENZYMES ( 170 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanI BbeI BbrPI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmFI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI Hin1I HindIII HpaI KasI KpnI Ksp632I KspI MaeI MaeII MamI MfeI MflI MluI MluNI MroI MscI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI StyI SwaI Tsp509I Tth111I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccII 102 CGCG CG^CG AseI 370 ATTAAT AT^TAAT AsnI 370 ATTAAT AT^TAAT AvaII 56 GGACC G^G[AT]CC BanII 34 GAGCCC G[GA]GC[CT]^C BbsI 237 GAAGAC GAAGAC(2/6) BbvCI 276 CCTCAGC CCTCAGC(-5/-2) BbvI 5 GCTGC GCAGC(8/12) BpuAI 237 GAAGAC GAAGAC(2/6) BsaI 250 GAGACC GGTCTC(1/5) BsgI 151 CTGCAC GTGCAG(16/14) BsmI 347 GAATGC GAATGC(1/-1) BsrI 255 CCAGT ACTGG(1/-1) BstUI 102 CGCG CG^CG DpnII 200 GATC ^GATC DraII 285 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoO109I 285 AGGCCCT [GA]G^G[ACGT]CC[CT] HaeII 112 AGCGCC [GA]GCGC^[CT] HgaI 100 GACGC GACGC(5/10) HincII 430 GTCAAC GT[CT]^[GA]AC HindII 430 GTCAAC GT[CT]^[GA]AC MboI 200 GATC ^GATC MseI 371 TTAA T^TAA MspA1I 129 CAGCGG C[AC]G^C[GT]G MvnI 102 CGCG CG^CG MwoI 8 GCGGTGGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NdeII 200 GATC ^GATC NlaIII 162 CATG CATG^ NspI 161 GCATGC [GA]CATG^[CT] PflMI 390 CCAACAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 94 GACTC GAGTC(4/5) PshAI 400 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 370 ATTAAT AT^TAAT Sau3AI 200 GATC ^GATC SfcI 203 CTACAG C^T[GA][CT]AG SmaI 59 CCCGGG CCC^GGG SphI 161 GCATGC GCATG^C Tru9I 371 TTAA T^TAA TseI 5 GCTGC G^C[AT]GC Tsp45I 364 GTCAC ^GT[GC]AC TspRI 256 CAGTG CA[GC]TG[ACGT][ACGT]^ Van91I 390 CCAACAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 297 CCAGAAACAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 59 CCCGGG C^CCGGG XmaI 59 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AluI 52 AGCT AG^CT 280 AGCT AvaI 59 CCCGGG C^[CT]CG[GA]G 156 CTCGGG BsmAI 64 GAGAC GTCTC(1/5) 250 GAGAC BsoBI 59 CCCGGG C^[CT]CG[GA]G 156 CTCGGG BstF5I 387 CATCC GGATG(2/0) 24 GGATG BstNI 77 CCTGG CC^[AT]GG 211 CCAGG CfoI 103 GCGC GCG^C 113 GCGC EcoRII 77 CCTGG ^CC[AT]GG 211 CCAGG FauI 38 CCCGC CCCGC(4/6) 142 GCGGG FokI 387 CATCC GGATG(9/13) 24 GGATG HhaI 103 GCGC GCG^C 113 GCGC HinP1I 103 GCGC G^CGC 113 GCGC HphI 83 TCACC GGTGA(8/7) 365 TCACC MaeIII 364 GTCAC ^GT[ACGT]AC 376 GTAAC MvaI 77 CCTGG CC^[AT]GG 211 CCAGG TaqI 32 TCGA T^CGA 408 TCGA TfiI 70 GAATC G^A[AT]TC 413 GAATC TthHB8I 32 TCGA T^CGA 408 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 25 enzymes) # ######################################### AciI BcnI BmyI BsaJI BsiYI BslI Bsp1286I BssKI Cac8I Cfr13I DdeI Fnu4HI HaeIII HapII HinfI HpaII ItaI MboII MnlI MspI NciI NlaIV Sau96I ScrFI SfaNI