################################## # NO CUT ENZYMES ( 150 enzymes) # ################################## AatII AccI AccIII AclI AcyI AflII AflIII AgeI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI AspHI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BclI BcnI BfaI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII CfoI ClaI CpoI DraIII DrdI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KspI MaeI MaeII MaeIII MamI MfeI MluI MroI MslI MspA1I MunI NaeI NarI NciI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tsp45I Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### Acc65I 308 GGTACC G^GTACC AccII 950 CGCG CG^CG AciI 951 GCGG CCGC(-3/-1) AhdI 19 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 925 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG Asp718I 308 GGTACC G^GTACC AspEI 19 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 672 TGGCCA TGG^CCA BanI 308 GGTACC G^G[CT][GA]CC BbsI 891 GTCTTC GAAGAC(2/6) BciVI 755 GGATAC GTATCC(6/5) BpuAI 891 GTCTTC GAAGAC(2/6) BsaI 1027 GAGACC GGTCTC(1/5) BseRI 1064 GAGGAG GAGGAG(10/8) BsmFI 108 GTCCC GGGAC(10/14) BsmI 767 GAATGC GAATGC(1/-1) BspHI 477 TCATGA T^CATGA BsrFI 116 ACCGGC [GA]^CCGG[CT] BstAPI 1106 GCAAATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 950 CGCG CG^CG Cac8I 190 GCCAGC GC[ACGT]^[ACGT]GC Cfr10I 116 ACCGGC [GA]^CCGG[CT] DraI 1053 TTTAAA TTT^AAA EaeI 672 TGGCCA [CT]^GGCC[GA] Eam1105I 19 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1017 GAAGAG CTCTTC(1/4) EcoRI 367 GAATTC G^AATTC HapII 117 CCGG C^CGG HpaII 117 CCGG C^CGG HphI 137 TCACC GGTGA(8/7) KpnI 308 GGTACC GGTAC^C Ksp632I 1017 GAAGAG CTCTTC(1/4) MluNI 672 TGGCCA TGG^CCA MscI 672 TGGCCA TGG^CCA MspI 117 CCGG C^CGG MvnI 950 CGCG CG^CG NdeI 803 CATATG CA^TATG PleI 889 GAGTC GAGTC(4/5) RcaI 477 TCATGA T^CATGA SspI 148 AATATT AAT^ATT TaqI 197 TCGA T^CGA TthHB8I 197 TCGA T^CGA XcmI 402 CCATAACTTTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### AfaI 309 GTAC GT^AC 750 GTAC ApaI 848 GGGCCC GGGCC^C 1122 GGGCCC BanII 848 GGGCCC G[GA]GC[CT]^C 1122 GGGCCC BbvI 54 GCTGC GCAGC(8/12) 871 GCTGC BglII 606 AGATCT A^GATCT 917 AGATCT BspMI 37 GCAGGT ACCTGC(4/8) 966 GCAGGT NcoI 14 CCATGG C^CATGG 642 CCATGG PstI 554 CTGCAG CTGCA^G 830 CTGCAG RsaI 309 GTAC GT^AC 750 GTAC SfcI 554 CTGCAG C^T[GA][CT]AG 830 CTGCAG TseI 54 GCTGC G^C[AT]GC 871 GCTGC ######################################### # 3 OR MORE CUT ENZYMES ( 54 enzymes) # ######################################### AcsI AluI AlwI ApoI Asp700I AvaII BmyI BpmI BsaJI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cfr13I DdeI DpnII DraII DsaI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI ItaI MboI MboII MflI MnlI MseI MvaI MwoI NdeII NlaIII NlaIV PflMI Sau3AI Sau96I ScrFI SfaNI StyI TfiI Tru9I Tsp509I TspRI Van91I XhoII XmnI