(Circular) (Six-base) MAPSORT of: pFN21AE6349.seq Check: 4931 from: 1 to: 5875 pFN21AE6349 5875 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2033 2033 Size: 5875 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3685 278 Size: 53 83 186 936 2149 2468 Fragments arranged by size: 2468 2149 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3218 1937 Size: 1281 4594 Acc65I G'GTAC_C Cuts at: 2313 3196 2313 Size: 883 4992 AclI AA'CG_TT Cuts at: 1563 4002 4375 1563 Size: 2439 373 3063 Fragments arranged by size: 3063 2439 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2883 3938 5322 5590 1632 Size: 1251 1055 1384 268 1917 Fragments arranged by size: 1917 1384 1251 1055 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5678 AflIII A'CryG_T Cuts at: 1203 4774 5751 1203 Size: 3571 977 1327 Fragments arranged by size: 3571 1327 977 AgeI A'CCGG_T Cuts at: 4706 4706 Size: 5875 AhdI GACnn_n'nnGTC Cuts at: 2029 4604 2029 Size: 2575 3300 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5875 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5875 AlwNI CAG_nnn'CTG Cuts at: 2935 3618 5190 2935 Size: 683 1572 3620 Fragments arranged by size: 3620 1572 683 ApaI G_GGCC'C Cuts at: 2857 3131 2857 Size: 274 5601 ApaLI G'TGCA_C Cuts at: 3932 5088 3932 Size: 1156 4719 ApoI r'AATT_y Cuts at: 1493 2138 2292 2372 3055 3184 3410 3562 5671 Size: 645 154 80 683 129 226 152 2109 Cuts at: 5671 1493 Size: 1697 Fragments arranged by size: 2109 1697 683 645 226 154 152 129 80 AseI AT'TA_AT Cuts at: 160 1032 4429 160 Size: 872 3397 1606 Fragments arranged by size: 3397 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5875 AvaI C'yCGr_G Cuts at: 1870 1943 3200 1870 Size: 73 1257 4545 Fragments arranged by size: 4545 1257 73 BamHI G'GATC_C Cuts at: 3205 3572 3205 Size: 367 5508 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2313 3196 4651 618 Size: 325 217 627 526 883 1455 1842 Fragments arranged by size: 1842 1455 883 627 526 325 217 BanII G_rGCy'C Cuts at: 1391 2857 3131 3194 1391 Size: 1466 274 63 4072 Fragments arranged by size: 4072 1466 274 63 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2889 928 Size: 971 990 3914 Fragments arranged by size: 3914 990 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4066 4100 1903 Size: 34 2129 34 3678 Fragments arranged by size: 3678 2129 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2754 3767 4977 2754 Size: 1013 1210 3652 Fragments arranged by size: 3652 1210 1013 BclI T'GATC_A Cuts at: 1548 1548 Size: 5875 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4486 136 Size: 107 122 71 631 605 2814 1525 Fragments arranged by size: 2814 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2611 2922 5870 2611 Size: 311 2948 2616 Fragments arranged by size: 2948 2616 311 BlpI GC'TnA_GC Cuts at: 3292 3292 Size: 5875 Bme1580I G_kGCm'C Cuts at: 1790 2817 2857 3131 3936 5092 1790 Size: 1027 40 274 805 1156 2573 Fragments arranged by size: 2573 1156 1027 805 274 40 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5875 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3606 4564 442 Size: 416 53 83 399 24 2189 958 1753 Fragments arranged by size: 2189 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5875 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2051 2892 2945 4535 1239 Size: 812 841 53 1590 2579 Fragments arranged by size: 2579 1590 841 812 53 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4000 4865 5163 4000 Size: 865 298 4712 Fragments arranged by size: 4712 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3026 4538 882 Size: 617 1527 1512 2219 Fragments arranged by size: 2219 1527 1512 617 BsaAI yAC'GTr Cuts at: 493 1204 3666 493 Size: 711 2462 2702 Fragments arranged by size: 2702 2462 711 BsaBI GATnn'nnATC Cuts at: 5869 5869 Size: 5875 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3682 4064 4699 275 Size: 53 83 186 936 2149 382 635 1451 Fragments arranged by size: 2149 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4308 4706 4980 5127 5538 4308 Size: 398 274 147 411 4645 Fragments arranged by size: 4645 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5650 Fragments arranged by size: 5650 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3084 1186 Size: 1898 3977 BseYI C'CCAG_C Cuts at: 5078 5574 5078 Size: 496 5379 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5302 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3325 4086 5114 1540 Size: 275 186 1324 761 1028 2301 Fragments arranged by size: 2301 1324 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3936 4021 5092 3936 Size: 85 1071 4719 Fragments arranged by size: 4719 1071 85 BsmI GAATG_Cn' Cuts at: 2778 3386 3479 2778 Size: 608 93 5174 Fragments arranged by size: 5174 608 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2817 2857 3131 3194 3936 4021 5092 Size: 399 1027 40 274 63 742 85 1071 Cuts at: 5092 1391 Size: 2174 Fragments arranged by size: 2174 1071 1027 742 399 274 85 63 40 BspEI T'CCGG_A Cuts at: 5538 5538 Size: 5875 BspHI T'CATG_A Cuts at: 2482 3764 5494 2482 Size: 1282 1730 2863 Fragments arranged by size: 2863 1730 1282 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2033 2962 3230 844 Size: 1189 929 268 3489 Fragments arranged by size: 3489 1189 929 268 BsrBI CCG'CTC Cuts at: 1394 1823 3762 1394 Size: 429 1939 3507 Fragments arranged by size: 3507 1939 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4370 4544 65 Size: 1183 3122 174 1396 Fragments arranged by size: 3122 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2121 4519 4706 1700 Size: 110 311 2398 187 2869 Fragments arranged by size: 2869 2398 311 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5875 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5875 BssSI C'ACGA_G Cuts at: 3935 4947 3935 Size: 1012 4863 BstAPI GCAn_nnn'nTGC Cuts at: 794 3117 794 Size: 2323 3552 BstBI TT'CG_AA Cuts at: 4679 4679 Size: 5875 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5875 BstYI r'GATC_y Cuts at: 1972 2075 2436 2611 2922 3205 3572 3957 3974 Size: 103 361 175 311 283 367 385 17 Cuts at: 3974 5426 5512 5524 5870 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 385 367 361 346 311 283 175 103 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2019 2647 2847 513 Size: 552 939 15 628 200 3541 Fragments arranged by size: 3541 939 628 552 200 15 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5136 Fragments arranged by size: 5136 683 56 BtsI GCAGTG_nn' Cuts at: 3391 4185 4205 3391 Size: 794 20 5061 Fragments arranged by size: 5061 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5875 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5840 DraI TTT'AAA Cuts at: 3060 3179 3060 Size: 119 5756 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4882 809 Size: 507 3566 1802 Fragments arranged by size: 3566 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2677 3322 4211 Size: 54 1425 50 275 18 847 645 889 Cuts at: 4211 5840 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 847 645 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3322 1537 Size: 275 1510 4090 Fragments arranged by size: 4090 1510 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3017 3805 1353 Size: 1664 788 3423 Fragments arranged by size: 3423 1664 788 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4462 4836 4982 387 Size: 1064 138 2873 374 146 1280 Fragments arranged by size: 2873 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2051 2883 2892 2945 3938 4535 5322 Size: 393 419 832 9 53 993 597 787 Cuts at: 5322 5590 1239 Size: 268 1524 Fragments arranged by size: 1524 993 832 787 597 419 393 268 53 9 EcoO109I rG'GnC_Cy Cuts at: 2853 2854 3127 3128 2853 Size: 1 273 1 5600 Fragments arranged by size: 5600 273 1 1 EcoRI G'AATT_C Cuts at: 2372 3184 3562 2372 Size: 812 378 4685 Fragments arranged by size: 4685 812 378 FspI TGC'GCA Cuts at: 4381 4381 Size: 5875 HaeII r_GCGC'y Cuts at: 1678 5022 1678 Size: 3344 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2015 2047 2934 2966 3063 Size: 32 135 32 443 32 887 32 97 Cuts at: 3063 3095 4516 4548 4590 4622 1373 Size: 32 1421 32 42 32 2626 Fragments arranged by size: 2626 1421 887 443 135 97 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3219 3465 669 Size: 988 281 1281 246 3079 Fragments arranged by size: 3079 1281 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5875 HpaI GTT'AAC Cuts at: 3465 3465 Size: 5875 KpnI G_GTAC'C Cuts at: 2317 3200 2317 Size: 883 4992 MfeI C'AATT_G Cuts at: 3474 3474 Size: 5875 MluI A'CGCG_T Cuts at: 5751 5751 Size: 5875 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4989 5173 1305 Size: 3684 184 2007 Fragments arranged by size: 3684 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2679 10 Size: 54 1425 343 847 3206 Fragments arranged by size: 3206 1425 847 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3833 4192 4351 518 Size: 648 2667 359 159 2042 Fragments arranged by size: 2667 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3291 3968 5116 5361 1606 Size: 1685 677 1148 245 2120 Fragments arranged by size: 2120 1685 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2019 2647 513 Size: 552 939 15 628 3741 Fragments arranged by size: 3741 939 628 552 15 NdeI CA'TA_TG Cuts at: 387 2809 387 Size: 2422 3453 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5875 NotI GC'GGCC_GC Cuts at: 3322 3322 Size: 5875 NspI r_CATG'y Cuts at: 1132 1229 3233 4778 1132 Size: 97 2004 1545 2229 Fragments arranged by size: 2229 2004 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5875 PciI A'CATG_T Cuts at: 4774 4774 Size: 5875 PflMI CCAn_nnn'nTGG Cuts at: 1108 2344 3004 3081 1108 Size: 1236 660 77 3902 Fragments arranged by size: 3902 1236 660 77 PfoI T'CCnGG_A Cuts at: 2961 2961 Size: 5875 PmeI GTTT'AAAC Cuts at: 3179 3179 Size: 5875 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3944 3976 5457 5489 3944 Size: 32 1481 32 4330 Fragments arranged by size: 4330 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5875 PsiI TTA'TAA Cuts at: 3445 3445 Size: 5875 PspOMI G'GGCC_C Cuts at: 2853 3127 2853 Size: 274 5601 PstI C_TGCA'G Cuts at: 830 2563 2839 3227 830 Size: 1733 276 388 3478 Fragments arranged by size: 3478 1733 388 276 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5875 SalI G'TCGA_C Cuts at: 1936 3217 1936 Size: 1281 4594 SbfI CC_TGCA'GG Cuts at: 3227 3227 Size: 5875 ScaI AGT'ACT Cuts at: 4123 5835 4123 Size: 1712 4163 SfcI C'TryA_G Cuts at: 826 1045 2559 2835 3223 4358 5039 5230 826 Size: 219 1514 276 388 1135 681 191 1471 Fragments arranged by size: 1514 1471 1135 681 388 276 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5875 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5875 SmaI CCC'GGG Cuts at: 1872 3202 1872 Size: 1330 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3979 4880 5142 820 Size: 197 926 2036 901 262 1553 Fragments arranged by size: 2036 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3666 493 Size: 3173 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5875 SphI G_CATG'C Cuts at: 1132 3233 1132 Size: 2101 3774 SspI AAT'ATT Cuts at: 5 52 2155 3799 5 Size: 47 2103 1644 2081 Fragments arranged by size: 2103 2081 1644 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5875 StyI C'CwwG_G Cuts at: 513 1065 2004 2019 2526 2647 3036 3163 3314 Size: 552 939 15 507 121 389 127 151 Cuts at: 3314 513 Size: 3074 Fragments arranged by size: 3074 939 552 507 389 151 127 121 15 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2992 3902 3919 4072 4257 4702 325 Size: 1255 1412 910 17 153 185 445 1498 Fragments arranged by size: 1498 1412 1255 910 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4121 5833 96 Size: 274 80 33 51 1443 2144 1712 138 Fragments arranged by size: 2144 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5835 5867 5835 Size: 32 5843 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5875 XbaI T'CTAG_A Cuts at: 3211 3211 Size: 5875 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2414 2414 Size: 5875 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5875 XmaI C'CCGG_G Cuts at: 1870 3200 1870 Size: 1330 4545 XmnI GAAnn'nnTTC Cuts at: 2499 2510 2535 4004 2499 Size: 11 25 1469 4370 Fragments arranged by size: 4370 1469 25 11 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3683 276 Size: 53 83 186 936 2149 2468 Fragments arranged by size: 2468 2149 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI