################################## # NO CUT ENZYMES ( 160 enzymes) # ################################## AatII AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspHI AspI AviII AvrII BalI BamHI BbeI BbrPI BciVI BclI BfrI BglII BlnI BsaAI BsaBI BsaHI BsaI BseAI BseRI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstXI BstYI BstZ17I Bsu36I Cfr10I ClaI CpoI DpnII DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HgaI Hin1I HindIII HpaI KasI KspI MaeII MamI MboI MfeI MflI MluI MluNI MroI MscI MseI MslI MunI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tru9I Tsp45I Tsp509I Tth111I Van91I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### Acc65I 620 GGTACC G^GTACC AccII 21 CGCG CG^CG AlwNI 185 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG Asp718I 620 GGTACC G^GTACC AvaI 69 CCCGGG C^[CT]CG[GA]G BbsI 376 GTCTTC GAAGAC(2/6) BbvCI 109 CCTCAGC CCTCAGC(-5/-2) BcgI 392 GCAACTACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 316 CTAG C^TAG BglI 66 GCCCCCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 574 GCTCAGC GC^T[ACGT]AGC BpmI 156 CTCCAG CTGGAG(16/14) Bpu1102I 574 GCTCAGC GC^T[ACGT]AGC BpuAI 376 GTCTTC GAAGAC(2/6) BsaWI 212 TCCGGT [AT]^CCGG[AT] BsoBI 69 CCCGGG C^[CT]CG[GA]G BstAPI 184 GCAGACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 565 GGTTACC G^GT[ACGT]ACC BstPI 565 GGTTACC G^GT[ACGT]ACC BstUI 21 CGCG CG^CG CelII 574 GCTCAGC GC^T[ACGT]AGC EaeI 176 CGGCCA [CT]^GGCC[GA] EarI 294 CTCTTC CTCTTC(1/4) EcoO65I 565 GGTTACC G^GT[ACGT]ACC EcoT14I 422 CCTTGG C^C[AT][AT]GG FauI 19 CCCGC CCCGC(4/6) KpnI 620 GGTACC GGTAC^C Ksp632I 294 CTCTTC CTCTTC(1/4) MaeI 316 CTAG C^TAG MaeIII 566 GTTAC ^GT[ACGT]AC MvnI 21 CGCG CG^CG PleI 292 GACTC GAGTC(4/5) PstI 612 CTGCAG CTGCA^G SfcI 612 CTGCAG C^T[GA][CT]AG SmaI 69 CCCGGG CCC^GGG SphI 85 GCATGC GCATG^C StyI 422 CCTTGG C^C[AT][AT]GG TaqI 401 TCGA T^CGA TthHB8I 401 TCGA T^CGA XcmI 456 CCATCTGAACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 69 CCCGGG C^CCGGG XmaI 69 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AvaII 357 GGTCC G^G[AT]CC 414 GGACC BanI 607 GGCACC G^G[CT][GA]CC 620 GGTACC BanII 9 GAGCCC G[GA]GC[CT]^C 403 GAGCCC BmyI 9 GAGCCC G[AGT]GC[ACT]^C 403 GAGCCC BsgI 182 GTGCAG GTGCAG(16/14) 427 GTGCAG Bsp1286I 9 GAGCCC G[AGT]GC[ACT]^C 403 GAGCCC BstF5I 381 CATCC GGATG(2/0) 264 GGATG DraII 356 AGGTCCT [GA]G^G[ACGT]CC[CT] 413 AGGACCT EcoO109I 356 AGGTCCT [GA]G^G[ACGT]CC[CT] 413 AGGACCT FokI 381 CATCC GGATG(9/13) 264 GGATG HincII 601 GTTGAC GT[CT]^[GA]AC 655 GTTGAC HindII 601 GTTGAC GT[CT]^[GA]AC 655 GTTGAC HphI 79 GGTGA GGTGA(8/7) 479 TCACC MboII 295 TCTTC GAAGA(8/7) 377 TCTTC MspA1I 90 CAGCTG C[AC]G^C[GT]G 615 CAGCTG NlaIII 86 CATG CATG^ 598 CATG NspI 85 GCATGC [GA]CATG^[CT] 597 GCATGT PpuMI 356 AGGTCCT [GA]G^G[AT]CC[CT] 413 AGGACCT PvuII 90 CAGCTG CAG^CTG 615 CAGCTG TfiI 141 GATTC G^A[AT]TC 444 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AciI AfaI AluI BbvI BcnI BsaJI BsiYI BslI BspMI BsrI BssKI BstNI Cac8I CfoI Cfr13I DdeI EcoRII Fnu4HI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MnlI MspI MvaI MwoI NciI NlaIV RsaI Sau96I ScrFI TseI TspRI