(Circular) (Six-base) MAPSORT of: pFN21AE1414.seq Check: 762 from: 1 to: 5377 pFN21AE1414 5377 bp With 182 enzymes: SgfI * July 6, 2012 17:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2498 2624 2646 2498 Size: 126 22 5229 Fragments arranged by size: 5229 126 22 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3187 278 Size: 53 83 186 936 1651 2468 Fragments arranged by size: 2468 1651 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2720 1937 Size: 783 4594 Acc65I G'GTAC_C Cuts at: 2625 2698 2625 Size: 73 5304 AclI AA'CG_TT Cuts at: 1563 3504 3877 1563 Size: 1941 373 3063 Fragments arranged by size: 3063 1941 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3440 4824 5092 1632 Size: 1808 1384 268 1917 Fragments arranged by size: 1917 1808 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5180 AflIII A'CryG_T Cuts at: 1203 4276 5253 1203 Size: 3073 977 1327 Fragments arranged by size: 3073 1327 977 AgeI A'CCGG_T Cuts at: 4208 4208 Size: 5377 AhdI GACnn_n'nnGTC Cuts at: 4106 4106 Size: 5377 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5377 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2113 1184 Size: 929 4448 AlwNI CAG_nnn'CTG Cuts at: 2195 3120 4692 2195 Size: 925 1572 2880 Fragments arranged by size: 2880 1572 925 ApaLI G'TGCA_C Cuts at: 3434 4590 3434 Size: 1156 4221 ApoI r'AATT_y Cuts at: 1493 2686 2912 3064 5173 1493 Size: 1193 226 152 2109 1697 Fragments arranged by size: 2109 1697 1193 226 152 AseI AT'TA_AT Cuts at: 160 1032 3931 160 Size: 872 2899 1606 Fragments arranged by size: 2899 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5377 AvaI C'yCGr_G Cuts at: 1870 1943 2074 2702 1870 Size: 73 131 628 4545 Fragments arranged by size: 4545 628 131 73 BamHI G'GATC_C Cuts at: 2707 3074 2707 Size: 367 5010 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2612 2625 2698 4153 618 Size: 325 217 627 825 13 73 1455 1842 Fragments arranged by size: 1842 1455 825 627 325 217 73 13 BanII G_rGCy'C Cuts at: 1391 2018 2412 2696 1391 Size: 627 394 284 4072 Fragments arranged by size: 4072 627 394 284 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2374 928 Size: 971 475 3931 Fragments arranged by size: 3931 971 475 BbvCI CC'TCA_GC Cuts at: 2115 2115 Size: 5377 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2386 2420 3568 3602 1903 Size: 34 50 34 365 34 1148 34 3678 Fragments arranged by size: 3678 1148 365 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3269 4479 3269 Size: 1210 4167 BclI T'GATC_A Cuts at: 1548 1548 Size: 5377 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2077 3988 136 Size: 107 122 71 631 605 405 1911 1525 Fragments arranged by size: 1911 1525 631 605 405 122 107 71 BglII A'GATC_T Cuts at: 5372 5372 Size: 5377 BlpI GC'TnA_GC Cuts at: 2580 2794 2580 Size: 214 5163 Bme1580I G_kGCm'C Cuts at: 1790 3438 4594 1790 Size: 1648 1156 2573 Fragments arranged by size: 2573 1648 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5377 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3108 4066 442 Size: 416 53 83 399 24 1691 958 1753 Fragments arranged by size: 1753 1691 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5377 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2146 4037 1239 Size: 907 1891 2579 Fragments arranged by size: 2579 1891 907 Bpu10I CC'TnA_GC Cuts at: 2115 2115 Size: 5377 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3502 4367 4665 3502 Size: 865 298 4214 Fragments arranged by size: 4214 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4040 882 Size: 617 2541 2219 Fragments arranged by size: 2541 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3168 493 Size: 711 1964 2702 Fragments arranged by size: 2702 1964 711 BsaBI GATnn'nnATC Cuts at: 5371 5371 Size: 5377 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3184 3566 4201 275 Size: 53 83 186 936 1651 382 635 1451 Fragments arranged by size: 1651 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2217 3810 4208 4482 4629 5040 2217 Size: 1593 398 274 147 411 2554 Fragments arranged by size: 2554 1593 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2408 2438 1178 Size: 30 165 30 1005 30 4117 Fragments arranged by size: 4117 1005 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5377 BseYI C'CCAG_C Cuts at: 2034 2080 4580 5076 2034 Size: 46 2500 496 2335 Fragments arranged by size: 2500 2335 496 46 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2208 2453 1163 Size: 573 472 245 4087 Fragments arranged by size: 4087 573 472 245 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2827 3588 4616 1540 Size: 275 186 826 761 1028 2301 Fragments arranged by size: 2301 1028 826 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3438 3523 4594 3438 Size: 85 1071 4221 Fragments arranged by size: 4221 1071 85 BsmI GAATG_Cn' Cuts at: 2888 2981 2888 Size: 93 5284 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2018 2412 2696 3438 3523 4594 1391 Size: 399 228 394 284 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 394 284 228 85 BspEI T'CCGG_A Cuts at: 5040 5040 Size: 5377 BspHI T'CATG_A Cuts at: 3266 4996 3266 Size: 1730 3647 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2498 2624 2646 2732 844 Size: 1654 126 22 86 3489 Fragments arranged by size: 3489 1654 126 86 22 BsrBI CCG'CTC Cuts at: 1394 1823 3264 1394 Size: 429 1441 3507 Fragments arranged by size: 3507 1441 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3872 4046 65 Size: 1183 2624 174 1396 Fragments arranged by size: 2624 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4021 4208 1700 Size: 110 2211 187 2869 Fragments arranged by size: 2869 2211 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5377 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5377 BssSI C'ACGA_G Cuts at: 3437 4449 3437 Size: 1012 4365 BstAPI GCAn_nnn'nTGC Cuts at: 794 2195 794 Size: 1401 3976 BstBI TT'CG_AA Cuts at: 4181 4181 Size: 5377 BstEII G'GTnAC_C Cuts at: 1184 2570 1184 Size: 1386 3991 BstYI r'GATC_y Cuts at: 1972 2707 3074 3459 3476 4928 5014 5026 5372 Size: 735 367 385 17 1452 86 12 346 Cuts at: 5372 1972 Size: 1977 Fragments arranged by size: 1977 1452 735 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4825 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4638 Fragments arranged by size: 4638 683 56 BtsI GCAGTG_nn' Cuts at: 2893 3687 3707 2893 Size: 794 20 4563 Fragments arranged by size: 4563 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5377 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5342 DraI TTT'AAA Cuts at: 2681 2681 Size: 5377 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4384 809 Size: 507 3068 1802 Fragments arranged by size: 3068 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2181 2824 3713 Size: 54 1425 50 275 18 351 643 889 Cuts at: 3713 5342 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 643 351 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2824 1537 Size: 275 1012 4090 Fragments arranged by size: 4090 1012 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2305 3307 1353 Size: 952 1002 3423 Fragments arranged by size: 3423 1002 952 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3964 4338 4484 387 Size: 1064 138 2375 374 146 1280 Fragments arranged by size: 2375 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2146 3440 4037 4824 5092 1239 Size: 393 514 1294 597 787 268 1524 Fragments arranged by size: 1524 1294 787 597 514 393 268 EcoO109I rG'GnC_Cy Cuts at: 2362 2419 2362 Size: 57 5320 EcoRI G'AATT_C Cuts at: 2686 3064 2686 Size: 378 4999 FspI TGC'GCA Cuts at: 3883 3883 Size: 5377 HaeII r_GCGC'y Cuts at: 1678 4524 1678 Size: 2846 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2408 2428 2440 2460 4018 Size: 32 135 32 836 20 12 20 1558 Cuts at: 4018 4050 4092 4124 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1558 836 135 42 32 32 32 32 20 20 12 HincII GTy'rAC Cuts at: 669 1657 1938 2608 2662 2721 2967 669 Size: 988 281 670 54 59 246 3079 Fragments arranged by size: 3079 988 670 281 246 59 54 HindIII A'AGCT_T Cuts at: 748 748 Size: 5377 HpaI GTT'AAC Cuts at: 2967 2967 Size: 5377 KpnI G_GTAC'C Cuts at: 2629 2702 2629 Size: 73 5304 MfeI C'AATT_G Cuts at: 2976 2976 Size: 5377 MluI A'CGCG_T Cuts at: 5253 5253 Size: 5377 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2532 4491 4675 1305 Size: 1227 1959 184 2007 Fragments arranged by size: 2007 1959 1227 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3555 Fragments arranged by size: 3555 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3335 3694 3853 518 Size: 648 2169 359 159 2042 Fragments arranged by size: 2169 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2097 2622 2793 3470 4618 4863 1606 Size: 491 525 171 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 525 491 245 171 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4825 NdeI CA'TA_TG Cuts at: 387 387 Size: 5377 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5377 NotI GC'GGCC_GC Cuts at: 2824 2824 Size: 5377 NspI r_CATG'y Cuts at: 1132 1229 2094 2606 2735 4280 1132 Size: 97 865 512 129 1545 2229 Fragments arranged by size: 2229 1545 865 512 129 97 PasI CC'CwG_GG Cuts at: 1348 2412 1348 Size: 1064 4313 PciI A'CATG_T Cuts at: 4276 4276 Size: 5377 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5377 PmeI GTTT'AAAC Cuts at: 2681 2681 Size: 5377 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2547 2579 3446 3478 4959 4991 2547 Size: 32 867 32 1481 32 2933 Fragments arranged by size: 2933 1481 867 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2362 2419 2362 Size: 57 5320 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5377 PsiI TTA'TAA Cuts at: 2947 2947 Size: 5377 PstI C_TGCA'G Cuts at: 830 2621 2729 830 Size: 1791 108 3478 Fragments arranged by size: 3478 1791 108 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5377 PvuII CAG'CTG Cuts at: 2097 2622 2097 Size: 525 4852 SalI G'TCGA_C Cuts at: 1936 2719 1936 Size: 783 4594 SbfI CC_TGCA'GG Cuts at: 2729 2729 Size: 5377 ScaI AGT'ACT Cuts at: 3625 5337 3625 Size: 1712 3665 SfcI C'TryA_G Cuts at: 826 1045 2617 2725 3860 4541 4732 826 Size: 219 1572 108 1135 681 191 1471 Fragments arranged by size: 1572 1471 1135 681 219 191 108 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5377 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5377 SmaI CCC'GGG Cuts at: 1872 2076 2704 1872 Size: 204 628 4545 Fragments arranged by size: 4545 628 204 SmlI C'TyrA_G Cuts at: 820 1017 1943 3481 4382 4644 820 Size: 197 926 1538 901 262 1553 Fragments arranged by size: 1553 1538 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3168 493 Size: 2675 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5377 SphI G_CATG'C Cuts at: 1132 2094 2735 1132 Size: 962 641 3774 Fragments arranged by size: 3774 962 641 SspI AAT'ATT Cuts at: 5 52 3301 5 Size: 47 3249 2081 Fragments arranged by size: 3249 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5377 StyI C'CwwG_G Cuts at: 513 1065 2427 2816 513 Size: 552 1362 389 3074 Fragments arranged by size: 3074 1362 552 389 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2026 2072 3404 3421 3574 3759 4204 Size: 1255 446 46 1332 17 153 185 445 Cuts at: 4204 325 Size: 1498 Fragments arranged by size: 1498 1332 1255 446 445 185 153 46 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3623 5335 96 Size: 274 80 33 51 1443 1646 1712 138 Fragments arranged by size: 1712 1646 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5337 5369 5337 Size: 32 5345 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5377 XbaI T'CTAG_A Cuts at: 2713 2713 Size: 5377 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2468 2468 Size: 5377 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5377 XmaI C'CCGG_G Cuts at: 1870 2074 2702 1870 Size: 204 628 4545 Fragments arranged by size: 4545 628 204 XmnI GAAnn'nnTTC Cuts at: 3506 3506 Size: 5377 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3185 276 Size: 53 83 186 936 1651 2468 Fragments arranged by size: 2468 1651 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI