(Linear) (Six-base) MAPSORT of: KIEE1414.seq Check: 1801 from: 1 to: 672 KIEE1414.orf With 182 enzymes: SgfI * July 3, 2012 16:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 493 619 641 672 Size: 493 126 22 31 Fragments arranged by size: 493 126 31 22 Acc65I G'GTAC_C Cuts at: 0 620 672 Size: 620 52 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 108 672 Size: 108 564 AlwNI CAG_nnn'CTG Cuts at: 0 190 672 Size: 190 482 AvaI C'yCGr_G Cuts at: 0 69 672 Size: 69 603 BanI G'GyrC_C Cuts at: 0 607 620 672 Size: 607 13 52 Fragments arranged by size: 607 52 13 BanII G_rGCy'C Cuts at: 0 13 407 672 Size: 13 394 265 Fragments arranged by size: 394 265 13 BbsI GAAGACnn'nnnn_ Cuts at: 0 369 672 Size: 369 303 BbvCI CC'TCA_GC Cuts at: 0 110 672 Size: 110 562 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 381 415 672 Size: 381 34 257 Fragments arranged by size: 381 257 34 BglI GCCn_nnn'nGGC Cuts at: 0 72 672 Size: 72 600 BlpI GC'TnA_GC Cuts at: 0 575 672 Size: 575 97 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 141 672 Size: 141 531 Bpu10I CC'TnA_GC Cuts at: 0 110 672 Size: 110 562 BsaWI w'CCGG_w Cuts at: 0 212 672 Size: 212 460 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 403 433 672 Size: 403 30 239 Fragments arranged by size: 403 239 30 BseYI C'CCAG_C Cuts at: 0 29 75 672 Size: 29 46 597 Fragments arranged by size: 597 46 29 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 203 448 672 Size: 203 245 224 Fragments arranged by size: 245 224 203 Bsp1286I G_dGCh'C Cuts at: 0 13 407 672 Size: 13 394 265 Fragments arranged by size: 394 265 13 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 493 619 641 672 Size: 493 126 22 31 Fragments arranged by size: 493 126 31 22 BstAPI GCAn_nnn'nTGC Cuts at: 0 190 672 Size: 190 482 BstEII G'GTnAC_C Cuts at: 0 565 672 Size: 565 107 EaeI y'GGCC_r Cuts at: 0 176 672 Size: 176 496 EarI CTCTTCn'nnn_ Cuts at: 0 300 672 Size: 300 372 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 141 672 Size: 141 531 EcoO109I rG'GnC_Cy Cuts at: 0 357 414 672 Size: 357 57 258 Fragments arranged by size: 357 258 57 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 403 423 435 455 672 Size: 403 20 12 20 217 Fragments arranged by size: 403 217 20 20 12 HincII GTy'rAC Cuts at: 0 603 657 672 Size: 603 54 15 Fragments arranged by size: 603 54 15 KpnI G_GTAC'C Cuts at: 0 624 672 Size: 624 48 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 527 672 Size: 527 145 MspA1I CmG'CkG Cuts at: 0 92 617 672 Size: 92 525 55 Fragments arranged by size: 525 92 55 NspI r_CATG'y Cuts at: 0 89 601 672 Size: 89 512 71 Fragments arranged by size: 512 89 71 PasI CC'CwG_GG Cuts at: 0 407 672 Size: 407 265 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 542 574 672 Size: 542 32 98 Fragments arranged by size: 542 98 32 PpuMI rG'GwC_Cy Cuts at: 0 357 414 672 Size: 357 57 258 Fragments arranged by size: 357 258 57 PstI C_TGCA'G Cuts at: 0 616 672 Size: 616 56 PvuII CAG'CTG Cuts at: 0 92 617 672 Size: 92 525 55 Fragments arranged by size: 525 92 55 SfcI C'TryA_G Cuts at: 0 612 672 Size: 612 60 SmaI CCC'GGG Cuts at: 0 71 672 Size: 71 601 SphI G_CATG'C Cuts at: 0 89 672 Size: 89 583 StyI C'CwwG_G Cuts at: 0 422 672 Size: 422 250 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 21 67 672 Size: 21 46 605 Fragments arranged by size: 605 46 21 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 463 672 Size: 463 209 XmaI C'CCGG_G Cuts at: 0 69 672 Size: 69 603 Enzymes that do cut: AarI Acc65I AlfI AlwNI AvaI BanI BanII BbsI BbvCI BcgI BglI BlpI BpmI Bpu10I BsaWI BsaXI BseYI BsgI Bsp1286I BspMI BstAPI BstEII EaeI EarI Eco57MI EcoO109I Hin4I HincII KpnI MmeI MspA1I NspI PasI PpiI PpuMI PstI PvuII SfcI SmaI SphI StyI TaqII XcmI XmaI Enzymes that do not cut: AatII AccI AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BciVI BclI BfrBI BglII Bme1580I BmgBI BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI BseRI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII HindIII HpaI KasI MfeI MluI MscI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PflMI PfoI PmeI PmlI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SrfI SspI StuI SwaI TatI TstI Tth111I XbaI XhoI XmnI ZraI