(Circular) (Six-base) MAPSORT of: pFN21AE6239.seq Check: 3990 from: 1 to: 5560 pFN21AE6239 5560 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2823 2823 Size: 5560 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3370 278 Size: 53 83 186 936 1834 2468 Fragments arranged by size: 2468 1834 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2903 1937 Size: 966 4594 Acc65I G'GTAC_C Cuts at: 2881 2881 Size: 5560 AclI AA'CG_TT Cuts at: 1563 3687 4060 1563 Size: 2124 373 3063 Fragments arranged by size: 3063 2124 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2868 3623 5007 5275 1632 Size: 1236 755 1384 268 1917 Fragments arranged by size: 1917 1384 1236 755 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5363 AflIII A'CryG_T Cuts at: 1203 4459 5436 1203 Size: 3256 977 1327 Fragments arranged by size: 3256 1327 977 AgeI A'CCGG_T Cuts at: 4391 4391 Size: 5560 AhdI GACnn_n'nnGTC Cuts at: 4289 4289 Size: 5560 AleI CACnn'nnGTG Cuts at: 1166 2594 1166 Size: 1428 4132 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5560 AlwNI CAG_nnn'CTG Cuts at: 2107 2221 2782 3303 4875 2107 Size: 114 561 521 1572 2792 Fragments arranged by size: 2792 1572 561 521 114 ApaI G_GGCC'C Cuts at: 2122 2132 2547 2122 Size: 10 415 5135 Fragments arranged by size: 5135 415 10 ApaLI G'TGCA_C Cuts at: 3617 4773 3617 Size: 1156 4404 ApoI r'AATT_y Cuts at: 1493 2869 3095 3247 5356 1493 Size: 1376 226 152 2109 1697 Fragments arranged by size: 2109 1697 1376 226 152 AseI AT'TA_AT Cuts at: 160 1032 4114 160 Size: 872 3082 1606 Fragments arranged by size: 3082 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5560 AvaI C'yCGr_G Cuts at: 1870 1943 2885 1870 Size: 73 942 4545 Fragments arranged by size: 4545 942 73 BamHI G'GATC_C Cuts at: 2890 3257 2890 Size: 367 5193 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2881 4336 618 Size: 325 217 627 1094 1455 1842 Fragments arranged by size: 1842 1455 1094 627 325 217 BanII G_rGCy'C Cuts at: 1391 2042 2122 2132 2174 2415 2547 2879 1391 Size: 651 80 10 42 241 132 332 4072 Fragments arranged by size: 4072 651 332 241 132 80 42 10 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4589 BbvCI CC'TCA_GC Cuts at: 2072 2072 Size: 5560 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2498 2532 3751 3785 1903 Size: 34 50 34 477 34 1219 34 3678 Fragments arranged by size: 3678 1219 477 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3452 4662 3452 Size: 1210 4350 BclI T'GATC_A Cuts at: 1548 1548 Size: 5560 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4171 136 Size: 107 122 71 631 605 2499 1525 Fragments arranged by size: 2499 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5555 5555 Size: 5560 BlpI GC'TnA_GC Cuts at: 2977 2977 Size: 5560 Bme1580I G_kGCm'C Cuts at: 1790 2122 2132 2547 3621 4777 1790 Size: 332 10 415 1074 1156 2573 Fragments arranged by size: 2573 1156 1074 415 332 10 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5560 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3291 4249 442 Size: 416 53 83 399 24 1874 958 1753 Fragments arranged by size: 1874 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2015 1056 Size: 959 4601 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2216 2390 2849 4220 1239 Size: 977 174 459 1371 2579 Fragments arranged by size: 2579 1371 977 459 174 Bpu10I CC'TnA_GC Cuts at: 2072 2273 2634 2072 Size: 201 361 4998 Fragments arranged by size: 4998 361 201 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2729 3685 4550 4848 2729 Size: 956 865 298 3441 Fragments arranged by size: 3441 956 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4223 882 Size: 617 2724 2219 Fragments arranged by size: 2724 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2145 2724 3351 493 Size: 711 941 579 627 2702 Fragments arranged by size: 2702 941 711 627 579 BsaBI GATnn'nnATC Cuts at: 2093 5554 2093 Size: 3461 2099 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3367 3749 4384 275 Size: 53 83 186 936 1834 382 635 1451 Fragments arranged by size: 1834 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2112 3993 4391 4665 4812 5223 2112 Size: 1881 398 274 147 411 2449 Fragments arranged by size: 2449 1881 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5335 Fragments arranged by size: 5335 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2258 1186 Size: 1072 4488 BseYI C'CCAG_C Cuts at: 2033 4763 5259 2033 Size: 2730 496 2334 Fragments arranged by size: 2730 2334 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4987 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3010 3771 4799 1540 Size: 275 186 1009 761 1028 2301 Fragments arranged by size: 2301 1028 1009 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2042 2174 2415 2664 3621 3706 4777 2042 Size: 132 241 249 957 85 1071 2825 Fragments arranged by size: 2825 1071 957 249 241 132 85 BsmI GAATG_Cn' Cuts at: 3071 3164 3071 Size: 93 5467 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2042 2122 2132 2174 2415 2547 2664 Size: 399 252 80 10 42 241 132 117 Cuts at: 2664 2879 3621 3706 4777 1391 Size: 215 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 252 241 215 132 117 85 80 42 10 BspEI T'CCGG_A Cuts at: 5223 5223 Size: 5560 BspHI T'CATG_A Cuts at: 3449 5179 3449 Size: 1730 3830 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2823 2915 844 Size: 1979 92 3489 Fragments arranged by size: 3489 1979 92 BsrBI CCG'CTC Cuts at: 1394 1823 3447 1394 Size: 429 1624 3507 Fragments arranged by size: 3507 1624 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2439 4055 4229 65 Size: 1183 1191 1616 174 1396 Fragments arranged by size: 1616 1396 1191 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4204 4391 1700 Size: 110 2394 187 2869 Fragments arranged by size: 2869 2394 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5560 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5560 BssSI C'ACGA_G Cuts at: 2414 2507 3620 4632 2414 Size: 93 1113 1012 3342 Fragments arranged by size: 3342 1113 1012 93 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5560 BstBI TT'CG_AA Cuts at: 4364 4364 Size: 5560 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5560 BstXI CCAn_nnnn'nTGG Cuts at: 2677 2677 Size: 5560 BstYI r'GATC_y Cuts at: 1972 2890 3257 3642 3659 5111 5197 5209 5555 Size: 918 367 385 17 1452 86 12 346 Cuts at: 5555 1972 Size: 1977 Fragments arranged by size: 1977 1452 918 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2391 513 Size: 552 1326 3682 Fragments arranged by size: 3682 1326 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4821 Fragments arranged by size: 4821 683 56 BtsI GCAGTG_nn' Cuts at: 3076 3870 3890 3076 Size: 794 20 4746 Fragments arranged by size: 4746 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5560 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5525 DraI TTT'AAA Cuts at: 2864 2864 Size: 5560 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4567 809 Size: 507 3251 1802 Fragments arranged by size: 3251 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2091 2610 3007 Size: 54 1425 50 275 18 261 519 397 Cuts at: 3007 3896 5525 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 519 397 275 261 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3007 1537 Size: 275 1195 4090 Fragments arranged by size: 4090 1195 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2190 2403 3490 1353 Size: 837 213 1087 3423 Fragments arranged by size: 3423 1087 837 213 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2182 2672 4147 4521 4667 387 Size: 1064 138 593 490 1475 374 146 1280 Fragments arranged by size: 1475 1280 1064 593 490 374 146 138 EcoICRI GAG'CTC Cuts at: 2040 2172 2413 2040 Size: 132 241 5187 Fragments arranged by size: 5187 241 132 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2216 2390 2849 2868 3623 4220 5007 Size: 393 584 174 459 19 755 597 787 Cuts at: 5007 5275 1239 Size: 268 1524 Fragments arranged by size: 1524 787 755 597 584 459 393 268 174 19 EcoO109I rG'GnC_Cy Cuts at: 2118 2119 2128 2129 2470 2543 2544 2779 2118 Size: 1 9 1 341 73 1 235 4899 Fragments arranged by size: 4899 341 235 73 9 1 1 1 EcoRI G'AATT_C Cuts at: 2869 3247 2869 Size: 378 5182 FspI TGC'GCA Cuts at: 2057 2819 4066 2057 Size: 762 1247 3551 Fragments arranged by size: 3551 1247 762 HaeII r_GCGC'y Cuts at: 1678 2581 2827 4707 1678 Size: 903 246 1880 2531 Fragments arranged by size: 2531 1880 903 246 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4201 4233 4275 4307 1373 Size: 32 135 32 2629 32 42 32 2626 Fragments arranged by size: 2629 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2904 3150 669 Size: 988 281 966 246 3079 Fragments arranged by size: 3079 988 966 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5560 HpaI GTT'AAC Cuts at: 3150 3150 Size: 5560 KpnI G_GTAC'C Cuts at: 2885 2885 Size: 5560 MfeI C'AATT_G Cuts at: 3159 3159 Size: 5560 MluI A'CGCG_T Cuts at: 5436 5436 Size: 5560 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4674 4858 1305 Size: 3369 184 2007 Fragments arranged by size: 3369 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2093 2612 10 Size: 54 1425 343 261 519 2958 Fragments arranged by size: 2958 1425 519 343 261 54 MslI CAynn'nnrTG Cuts at: 518 1166 2594 2675 3518 3877 4036 518 Size: 648 1428 81 843 359 159 2042 Fragments arranged by size: 2042 1428 843 648 359 159 81 MspA1I CmG'CkG Cuts at: 1606 2976 3653 4801 5046 1606 Size: 1370 677 1148 245 2120 Fragments arranged by size: 2120 1370 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2391 513 Size: 552 1326 3682 Fragments arranged by size: 3682 1326 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5560 NheI G'CTAG_C Cuts at: 1052 2011 1052 Size: 959 4601 NotI GC'GGCC_GC Cuts at: 3007 3007 Size: 5560 NspI r_CATG'y Cuts at: 1132 1229 2056 2632 2918 4463 1132 Size: 97 827 576 286 1545 2229 Fragments arranged by size: 2229 1545 827 576 286 97 PasI CC'CwG_GG Cuts at: 1348 2122 1348 Size: 774 4786 PciI A'CATG_T Cuts at: 4459 4459 Size: 5560 PflMI CCAn_nnn'nTGG Cuts at: 1108 2221 2392 1108 Size: 1113 171 4276 Fragments arranged by size: 4276 1113 171 PmeI GTTT'AAAC Cuts at: 2864 2864 Size: 5560 PmlI CAC'GTG Cuts at: 2145 2145 Size: 5560 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3629 3661 5142 5174 3629 Size: 32 1481 32 4015 Fragments arranged by size: 4015 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2470 2779 2470 Size: 309 5251 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5560 PsiI TTA'TAA Cuts at: 3130 3130 Size: 5560 PspOMI G'GGCC_C Cuts at: 2118 2128 2543 2118 Size: 10 415 5135 Fragments arranged by size: 5135 415 10 PstI C_TGCA'G Cuts at: 830 2478 2912 830 Size: 1648 434 3478 Fragments arranged by size: 3478 1648 434 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5560 SacI G_AGCT'C Cuts at: 2042 2174 2415 2042 Size: 132 241 5187 Fragments arranged by size: 5187 241 132 SalI G'TCGA_C Cuts at: 1936 2902 1936 Size: 966 4594 SapI GCTCTTCn'nnn_ Cuts at: 2403 2403 Size: 5560 SbfI CC_TGCA'GG Cuts at: 2912 2912 Size: 5560 ScaI AGT'ACT Cuts at: 3808 5520 3808 Size: 1712 3848 SexAI A'CCwGG_T Cuts at: 2154 2535 2775 2154 Size: 381 240 4939 Fragments arranged by size: 4939 381 240 SfcI C'TryA_G Cuts at: 826 1045 2044 2206 2474 2908 4043 4724 4915 Size: 219 999 162 268 434 1135 681 191 Cuts at: 4915 826 Size: 1471 Fragments arranged by size: 1471 1135 999 681 434 268 219 191 162 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5560 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5560 SmaI CCC'GGG Cuts at: 1872 2887 1872 Size: 1015 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2744 3664 4565 4827 820 Size: 197 926 801 920 901 262 1553 Fragments arranged by size: 1553 926 920 901 801 262 197 SnaBI TAC'GTA Cuts at: 493 3351 493 Size: 2858 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5560 SphI G_CATG'C Cuts at: 1132 2918 1132 Size: 1786 3774 SspI AAT'ATT Cuts at: 5 52 3484 5 Size: 47 3432 2081 Fragments arranged by size: 3432 2081 47 StuI AGG'CCT Cuts at: 1517 2261 1517 Size: 744 4816 StyI C'CwwG_G Cuts at: 513 1065 2262 2391 2999 513 Size: 552 1197 129 608 3074 Fragments arranged by size: 3074 1197 608 552 129 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3587 3604 3757 3942 4387 325 Size: 1255 2007 17 153 185 445 1498 Fragments arranged by size: 2007 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3806 5518 96 Size: 274 80 33 51 1443 1829 1712 138 Fragments arranged by size: 1829 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2523 2555 5520 5552 2523 Size: 32 2965 32 2531 Fragments arranged by size: 2965 2531 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5560 XbaI T'CTAG_A Cuts at: 2896 2896 Size: 5560 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2596 2596 Size: 5560 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5560 XmaI C'CCGG_G Cuts at: 1870 2885 1870 Size: 1015 4545 XmnI GAAnn'nnTTC Cuts at: 3689 3689 Size: 5560 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3368 276 Size: 53 83 186 936 1834 2468 Fragments arranged by size: 2468 1834 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspXI PsrI PvuII RsrII SacII SanDI SfiI SfoI SrfI SwaI