Restriction map for
pFN21AE6331
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# NO CUT ENZYMES ( 150 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AviII AvrII BamHI
BbeI BbvCI BclI BcnI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BsaHI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MluI MroI MseI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI Tru9I TthHB8I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccI 946 GTCTAC GT^[AC][GT]AC
AlwI 5 GGATC GGATC(4/5)
AspI 187 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 500 CTCGGG C^[CT]CG[GA]G
BbrPI 333 CACGTG CAC^GTG
BbsI 924 GAAGAC GAAGAC(2/6)
BcgI 365 GCAACCAAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 286 GTATCC GTATCC(6/5)
BpuAI 924 GAAGAC GAAGAC(2/6)
BsaBI 106 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 320 ACCGGA [AT]^CCGG[AT]
BsoBI 500 CTCGGG C^[CT]CG[GA]G
BstYI 5 GGATCT [GA]^GATC[CT]
DraII 90 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 899 CCATGG C^C[GA][CT]GG
EcoNI 94 CCTTGGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 90 AGGACCT [GA]G^G[ACGT]CC[CT]
FauI 1133 CCCGC CCCGC(4/6)
HapII 321 CCGG C^CGG
HpaII 321 CCGG C^CGG
MamI 106 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 295 CAATTG C^AATTG
MflI 5 GGATCT [GA]^GATC[CT]
MspA1I 444 CAGCGG C[AC]G^C[GT]G
MspI 321 CCGG C^CGG
MunI 295 CAATTG C^AATTG
NcoI 899 CCATGG C^CATGG
PflFI 187 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 333 CACGTG CAC^GTG
PmlI 333 CACGTG CAC^GTG
PpuMI 90 AGGACCT [GA]G^G[AT]CC[CT]
SacI 394 GAGCTC GAGCT^C
SfcI 764 CTACAG C^T[GA][CT]AG
SmlI 709 CTCAAG C^T[CT][GA]AG
Tsp509I 296 AATT ^AATT
Tth111I 187 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 5 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
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AflIII 332 ACACGT A^C[GA][CT]GT
334 ACGTGT
AluI 395 AGCT AG^CT
704 AGCT
AlwNI 185 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
1051 CAGAATCTG
AspHI 300 GAGCAC G[AT]GC[AT]^C
394 GAGCTC
AvaII 91 GGACC G^G[AT]CC
788 GGACC
BalI 1007 TGGCCA TGG^CCA
1172 TGGCCA
BanII 394 GAGCTC G[GA]GC[CT]^C
1078 GAGCCC
BglI 644 GCCAGGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
970 GCCAGAGAGGC
BsaAI 206 TACGTG [CT]AC^GT[GA]
333 CACGTG
BsaI 221 GAGACC GGTCTC(1/5)
817 GAGACC
BseRI 509 CTCCTC GAGGAG(10/8)
696 CTCCTC
BsgI 120 CTGCAC GTGCAG(16/14)
630 CTGCAC
BsiHKAI 300 GAGCAC G[AT]GC[AT]^C
394 GAGCTC
BsmFI 782 GGGAC GGGAC(10/14)
59 GTCCC
EarI 213 CTCTTC CTCTTC(1/4)
589 CTCTTC
Ksp632I 213 CTCTTC CTCTTC(1/4)
589 CTCTTC
MluNI 1007 TGGCCA TGG^CCA
1172 TGGCCA
MscI 1007 TGGCCA TGG^CCA
1172 TGGCCA
MslI 792 CACTGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
999 CATGAGGGTG
PflMI 150 CCAAGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1050 CCAGAATCTGG
TfiI 111 GAATC G^A[AT]TC
1053 GAATC
Van91I 150 CCAAGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1050 CCAGAATCTGG
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# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AfaI BanI BbvI BmyI
BpmI BsaJI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
BstXI Cac8I Cfr13I DdeI DpnII
EaeI EcoRII EcoT14I Fnu4HI FokI
HaeIII HinfI HphI ItaI MaeII
MaeIII MboI MboII MnlI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI RsaI Sau3AI Sau96I ScrFI
StyI TseI Tsp45I TspRI