Restriction map for
pFN21AE6331
(Circular) (Six-base) MAPSORT of: pFN21AE6331.seq Check: 9243 from: 1 to: 5902
pFN21AE6331 5902 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3712 278
Size: 53 83 186 936 2176 2468
Fragments arranged by size:
2468 2176 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2952 3245 1937
Size: 1015 293 4594
Fragments arranged by size:
4594 1015 293
Acc65I G'GTAC_C
Cuts at: 3223 3223
Size: 5902
AclI AA'CG_TT
Cuts at: 1563 4029 4402 1563
Size: 2466 373 3063
Fragments arranged by size:
3063 2466 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3965 5349 5617 1632
Size: 2333 1384 268 1917
Fragments arranged by size:
2333 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5705
AflIII A'CryG_T
Cuts at: 1203 2337 2339 4801 5778 1203
Size: 1134 2 2462 977 1327
Fragments arranged by size:
2462 1327 1134 977 2
AgeI A'CCGG_T
Cuts at: 4733 4733
Size: 5902
AhdI GACnn_n'nnGTC
Cuts at: 4631 4631
Size: 5902
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5902
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3046 1184
Size: 1862 4040
AlwNI CAG_nnn'CTG
Cuts at: 2195 3061 3645 5217 2195
Size: 866 584 1572 2880
Fragments arranged by size:
2880 1572 866 584
ApaLI G'TGCA_C
Cuts at: 3959 5115 3959
Size: 1156 4746
ApoI r'AATT_y
Cuts at: 1493 3211 3437 3589 5698 1493
Size: 1718 226 152 2109 1697
Fragments arranged by size:
2109 1718 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4456 160
Size: 872 3424 1606
Fragments arranged by size:
3424 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5902
AvaI C'yCGr_G
Cuts at: 1870 1943 2505 3227 1870
Size: 73 562 722 4545
Fragments arranged by size:
4545 722 562 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3086 3119 3086
Size: 33 5869
BamHI G'GATC_C
Cuts at: 3232 3599 3232
Size: 367 5535
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2037 2467 2731 3223 4678
Size: 325 217 627 250 430 264 492 1455
Cuts at: 4678 618
Size: 1842
Fragments arranged by size:
1842 1455 627 492 430 325 264 250
217
BanII G_rGCy'C
Cuts at: 1391 2403 3087 3221 1391
Size: 1012 684 134 4072
Fragments arranged by size:
4072 1012 684 134
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2936 928
Size: 971 1037 3894
Fragments arranged by size:
3894 1037 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2359 2393 4093 4127 1903
Size: 34 422 34 1700 34 3678
Fragments arranged by size:
3678 1700 422 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2302 3794 5004 2302
Size: 1492 1210 3200
Fragments arranged by size:
3200 1492 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5902
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2655 2981 4513
Size: 107 122 71 631 605 983 326 1532
Cuts at: 4513 136
Size: 1525
Fragments arranged by size:
1532 1525 983 631 605 326 122 107
71
BglII A'GATC_T
Cuts at: 5897 5897
Size: 5902
BlpI GC'TnA_GC
Cuts at: 3319 3319
Size: 5902
Bme1580I G_kGCm'C
Cuts at: 1790 2040 2472 2971 3963 5119 1790
Size: 250 432 499 992 1156 2573
Fragments arranged by size:
2573 1156 992 499 432 250
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5902
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2027 3633 4591
Size: 416 53 83 399 24 610 1606 958
Cuts at: 4591 442
Size: 1753
Fragments arranged by size:
1753 1606 958 610 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5902
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2244 2333 3015 3019 4562 1239
Size: 1005 89 682 4 1543 2579
Fragments arranged by size:
2579 1543 1005 682 89 4
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2699 4027 4892 5190 2699
Size: 1328 865 298 3411
Fragments arranged by size:
3411 1328 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2220 2816 4565 882
Size: 617 721 596 1749 2219
Fragments arranged by size:
2219 1749 721 617 596
BsaAI yAC'GTr
Cuts at: 493 1204 2213 2340 3693 493
Size: 711 1009 127 1353 2702
Fragments arranged by size:
2702 1353 1009 711 127
BsaBI GATnn'nnATC
Cuts at: 2115 5896 2115
Size: 3781 2121
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3709 4091 4726 275
Size: 53 83 186 936 2176 382 635 1451
Fragments arranged by size:
2176 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2325 4335 4733 5007 5154 5565 2325
Size: 2010 398 274 147 411 2662
Fragments arranged by size:
2662 2010 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2609 2639 1178
Size: 30 165 30 1206 30 4441
Fragments arranged by size:
4441 1206 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2505 2692 1186
Size: 1319 187 4396
Fragments arranged by size:
4396 1319 187
BseYI C'CCAG_C
Cuts at: 2831 5105 5601 2831
Size: 2274 496 3132
Fragments arranged by size:
3132 2274 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2110 2620 1163
Size: 573 374 510 4445
Fragments arranged by size:
4445 573 510 374
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3352 4113 5141 1540
Size: 275 186 1351 761 1028 2301
Fragments arranged by size:
2301 1351 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2309 2403 3963 4048 5119 2309
Size: 94 1560 85 1071 3092
Fragments arranged by size:
3092 1560 1071 94 85
BsmI GAATG_Cn'
Cuts at: 3413 3506 3413
Size: 93 5809
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2040 2309 2403 2472 2971 3087 3221
Size: 399 250 269 94 69 499 116 134
Cuts at: 3221 3963 4048 5119 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 499 399 269 250 134
116 94 85 69
BspEI T'CCGG_A
Cuts at: 5565 5565
Size: 5902
BspHI T'CATG_A
Cuts at: 3791 5521 3791
Size: 1730 4172
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3257 844
Size: 2413 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3789 1394
Size: 429 1966 3507
Fragments arranged by size:
3507 1966 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4397 4571 65
Size: 1183 3149 174 1396
Fragments arranged by size:
3149 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4546 4733 1700
Size: 110 2736 187 2869
Fragments arranged by size:
2869 2736 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5902
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5902
BssSI C'ACGA_G
Cuts at: 3962 4974 3962
Size: 1012 4890
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5902
BstBI TT'CG_AA
Cuts at: 4706 4706
Size: 5902
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5902
BstXI CCAn_nnnn'nTGG
Cuts at: 2803 3010 3125 2803
Size: 207 115 5580
Fragments arranged by size:
5580 207 115
BstYI r'GATC_y
Cuts at: 1972 2010 3232 3599 3984 4001 5453 5539 5551
Size: 38 1222 367 385 17 1452 86 12
Cuts at: 5551 5897 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1222 385 367 346 86 38
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2904 513
Size: 552 939 900 3511
Fragments arranged by size:
3511 939 900 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3097 487
Size: 683 56 1871 3292
Fragments arranged by size:
3292 1871 683 56
BtsI GCAGTG_nn'
Cuts at: 2637 3418 4212 4232 2637
Size: 781 794 20 4307
Fragments arranged by size:
4307 794 781 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5902
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5867
DraI TTT'AAA
Cuts at: 3206 3206
Size: 5902
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4909 809
Size: 507 3593 1802
Fragments arranged by size:
3593 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2059 3012 3177
Size: 54 1425 50 275 18 229 953 165
Cuts at: 3177 3349 4238 5867 8
Size: 172 889 1629 43
Fragments arranged by size:
1629 1425 953 889 275 229 172 165
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3349 1537
Size: 275 1537 4090
Fragments arranged by size:
4090 1537 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2224 2600 3832 1353
Size: 871 376 1232 3423
Fragments arranged by size:
3423 1232 871 376
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4489 4863 5009 387
Size: 1064 138 2900 374 146 1280
Fragments arranged by size:
2900 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2401 2401
Size: 5902
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2244 2333 3015 3019 3965 4562 5349
Size: 393 612 89 682 4 946 597 787
Cuts at: 5349 5617 1239
Size: 268 1524
Fragments arranged by size:
1524 946 787 682 612 597 393 268
89 4
EcoNI CCTnn'n_nnAGG
Cuts at: 2103 2103
Size: 5902
EcoO109I rG'GnC_Cy
Cuts at: 2096 2096
Size: 5902
EcoRI G'AATT_C
Cuts at: 3211 3589 3211
Size: 378 5524
FspI TGC'GCA
Cuts at: 4408 4408
Size: 5902
HaeII r_GCGC'y
Cuts at: 1678 5049 1678
Size: 3371 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2217 2249 2609 2641 4543
Size: 32 135 32 645 32 360 32 1902
Cuts at: 4543 4575 4617 4649 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1902 645 360 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3246 3492 669
Size: 988 281 1308 246 3079
Fragments arranged by size:
3079 1308 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5902
HpaI GTT'AAC
Cuts at: 3492 3492
Size: 5902
KpnI G_GTAC'C
Cuts at: 3227 3227
Size: 5902
MfeI C'AATT_G
Cuts at: 2300 3501 2300
Size: 1201 4701
MluI A'CGCG_T
Cuts at: 5778 5778
Size: 5902
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2906 5016 5200 1305
Size: 1601 2110 184 2007
Fragments arranged by size:
2110 2007 1601 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3014 3179 10
Size: 54 1425 343 1182 165 2733
Fragments arranged by size:
2733 1425 1182 343 165 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2801 3008 3860 4219 4378 518
Size: 648 1635 207 852 359 159 2042
Fragments arranged by size:
2042 1635 852 648 359 207 159
MspA1I CmG'CkG
Cuts at: 1606 2451 3318 3995 5143 5388 1606
Size: 845 867 677 1148 245 2120
Fragments arranged by size:
2120 1148 867 845 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2904 513
Size: 552 939 900 3511
Fragments arranged by size:
3511 939 900 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5902
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5902
NotI GC'GGCC_GC
Cuts at: 3349 3349
Size: 5902
NspI r_CATG'y
Cuts at: 1132 1229 2267 2951 2975 3260 4805 1132
Size: 97 1038 684 24 285 1545 2229
Fragments arranged by size:
2229 1545 1038 684 285 97 24
PasI CC'CwG_GG
Cuts at: 1348 2473 1348
Size: 1125 4777
PciI A'CATG_T
Cuts at: 4801 4801
Size: 5902
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2161 3061 1108
Size: 1053 900 3949
Fragments arranged by size:
3949 1053 900
PfoI T'CCnGG_A
Cuts at: 2070 2281 2070
Size: 211 5691
PmeI GTTT'AAAC
Cuts at: 3206 3206
Size: 5902
PmlI CAC'GTG
Cuts at: 2340 2340
Size: 5902
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3971 4003 5484 5516 3971
Size: 32 1481 32 4357
Fragments arranged by size:
4357 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2096 2096
Size: 5902
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5902
PsiI TTA'TAA
Cuts at: 3472 3472
Size: 5902
PstI C_TGCA'G
Cuts at: 830 3254 830
Size: 2424 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5902
SacI G_AGCT'C
Cuts at: 2403 2403
Size: 5902
SalI G'TCGA_C
Cuts at: 1936 3244 1936
Size: 1308 4594
SbfI CC_TGCA'GG
Cuts at: 3254 3254
Size: 5902
ScaI AGT'ACT
Cuts at: 4150 5862 4150
Size: 1712 4190
SfcI C'TryA_G
Cuts at: 826 1045 2769 3250 4385 5066 5257 826
Size: 219 1724 481 1135 681 191 1471
Fragments arranged by size:
1724 1471 1135 681 481 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5902
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5902
SmaI CCC'GGG
Cuts at: 1872 3229 1872
Size: 1357 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2714 4006 4907 5169 820
Size: 197 926 771 1292 901 262 1553
Fragments arranged by size:
1553 1292 926 901 771 262 197
SnaBI TAC'GTA
Cuts at: 493 3693 493
Size: 3200 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5902
SphI G_CATG'C
Cuts at: 1132 3260 1132
Size: 2128 3774
SspI AAT'ATT
Cuts at: 5 52 3826 5
Size: 47 3774 2081
Fragments arranged by size:
3774 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5902
StyI C'CwwG_G
Cuts at: 513 1065 2004 2099 2643 2815 2904 3341 513
Size: 552 939 95 544 172 89 437 3074
Fragments arranged by size:
3074 939 552 544 437 172 95 89
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2168 3929 3946 4099 4284 4729 325
Size: 1255 588 1761 17 153 185 445 1498
Fragments arranged by size:
1761 1498 1255 588 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4148 5860 96
Size: 274 80 33 51 1443 2171 1712 138
Fragments arranged by size:
2171 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5862 5894 5862
Size: 32 5870
Tth111I GACn'n_nGTC
Cuts at: 1316 2195 1316
Size: 879 5023
XbaI T'CTAG_A
Cuts at: 3238 3238
Size: 5902
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5902
XmaI C'CCGG_G
Cuts at: 1870 3227 1870
Size: 1357 4545
XmnI GAAnn'nnTTC
Cuts at: 4031 4031
Size: 5902
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3710 276
Size: 53 83 186 936 2176 2468
Fragments arranged by size:
2468 2176 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PstI PvuI SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PspOMI PspXI PsrI PvuII RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI