(Linear) (Six-base) MAPSORT of: KIEE6331.seq Check: 4065 from: 1 to: 1197 KIEE6331.orf With 182 enzymes: SgfI * March 16, 2015 11:29 .. AccI GT'mk_AC Cuts at: 0 947 1197 Size: 947 250 AflIII A'CryG_T Cuts at: 0 332 334 1197 Size: 332 2 863 Fragments arranged by size: 863 332 2 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1041 1197 Size: 1041 156 AlwNI CAG_nnn'CTG Cuts at: 0 190 1056 1197 Size: 190 866 141 Fragments arranged by size: 866 190 141 AvaI C'yCGr_G Cuts at: 0 500 1197 Size: 500 697 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1081 1114 1197 Size: 1081 33 83 Fragments arranged by size: 1081 83 33 BanI G'GyrC_C Cuts at: 0 32 462 726 1197 Size: 32 430 264 471 Fragments arranged by size: 471 430 264 32 BanII G_rGCy'C Cuts at: 0 398 1082 1197 Size: 398 684 115 Fragments arranged by size: 684 398 115 BbsI GAAGACnn'nnnn_ Cuts at: 0 931 1197 Size: 931 266 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 354 388 1197 Size: 354 34 809 Fragments arranged by size: 809 354 34 BciVI GTATCCnnnnn_n' Cuts at: 0 297 1197 Size: 297 900 BglI GCCn_nnn'nGGC Cuts at: 0 650 976 1197 Size: 650 326 221 Fragments arranged by size: 650 326 221 Bme1580I G_kGCm'C Cuts at: 0 35 467 966 1197 Size: 35 432 499 231 Fragments arranged by size: 499 432 231 35 BmrI ACTGGGnnnn_n' Cuts at: 0 22 1197 Size: 22 1175 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 239 328 1010 1014 1197 Size: 239 89 682 4 183 Fragments arranged by size: 682 239 183 89 4 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 694 1197 Size: 694 503 BsaI GGTCTCn'nnnn_ Cuts at: 0 215 811 1197 Size: 215 596 386 Fragments arranged by size: 596 386 215 BsaAI yAC'GTr Cuts at: 0 208 335 1197 Size: 208 127 862 Fragments arranged by size: 862 208 127 BsaBI GATnn'nnATC Cuts at: 0 110 1197 Size: 110 1087 BsaWI w'CCGG_w Cuts at: 0 320 1197 Size: 320 877 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 604 634 1197 Size: 604 30 563 Fragments arranged by size: 604 563 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 500 687 1197 Size: 500 187 510 Fragments arranged by size: 510 500 187 BseYI C'CCAG_C Cuts at: 0 826 1197 Size: 826 371 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 105 615 1197 Size: 105 510 582 Fragments arranged by size: 582 510 105 BsiHKAI G_wGCw'C Cuts at: 0 304 398 1197 Size: 304 94 799 Fragments arranged by size: 799 304 94 Bsp1286I G_dGCh'C Cuts at: 0 35 304 398 467 966 1082 1197 Size: 35 269 94 69 499 116 115 Fragments arranged by size: 499 269 116 115 94 69 35 BstXI CCAn_nnnn'nTGG Cuts at: 0 798 1005 1120 1197 Size: 798 207 115 77 Fragments arranged by size: 798 207 115 77 BstYI r'GATC_y Cuts at: 0 5 1197 Size: 5 1192 BtgI C'CryG_G Cuts at: 0 899 1197 Size: 899 298 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1092 1197 Size: 1092 105 BtsI GCAGTG_nn' Cuts at: 0 632 1197 Size: 632 565 EaeI y'GGCC_r Cuts at: 0 54 1007 1172 1197 Size: 54 953 165 25 Fragments arranged by size: 953 165 54 25 EarI CTCTTCn'nnn_ Cuts at: 0 219 595 1197 Size: 219 376 602 Fragments arranged by size: 602 376 219 EcoICRI GAG'CTC Cuts at: 0 396 1197 Size: 396 801 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 239 328 1010 1014 1197 Size: 239 89 682 4 183 Fragments arranged by size: 682 239 183 89 4 EcoNI CCTnn'n_nnAGG Cuts at: 0 98 1197 Size: 98 1099 EcoO109I rG'GnC_Cy Cuts at: 0 91 1197 Size: 91 1106 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 212 244 604 636 1197 Size: 212 32 360 32 561 Fragments arranged by size: 561 360 212 32 32 MfeI C'AATT_G Cuts at: 0 295 1197 Size: 295 902 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 901 1197 Size: 901 296 MscI TGG'CCA Cuts at: 0 1009 1174 1197 Size: 1009 165 23 Fragments arranged by size: 1009 165 23 MslI CAynn'nnrTG Cuts at: 0 796 1003 1197 Size: 796 207 194 Fragments arranged by size: 796 207 194 MspA1I CmG'CkG Cuts at: 0 446 1197 Size: 446 751 NcoI C'CATG_G Cuts at: 0 899 1197 Size: 899 298 NspI r_CATG'y Cuts at: 0 262 946 970 1197 Size: 262 684 24 227 Fragments arranged by size: 684 262 227 24 PasI CC'CwG_GG Cuts at: 0 468 1197 Size: 468 729 PflMI CCAn_nnn'nTGG Cuts at: 0 156 1056 1197 Size: 156 900 141 Fragments arranged by size: 900 156 141 PfoI T'CCnGG_A Cuts at: 0 65 276 1197 Size: 65 211 921 Fragments arranged by size: 921 211 65 PmlI CAC'GTG Cuts at: 0 335 1197 Size: 335 862 PpuMI rG'GwC_Cy Cuts at: 0 91 1197 Size: 91 1106 SacI G_AGCT'C Cuts at: 0 398 1197 Size: 398 799 SfcI C'TryA_G Cuts at: 0 764 1197 Size: 764 433 SmlI C'TyrA_G Cuts at: 0 709 1197 Size: 709 488 StyI C'CwwG_G Cuts at: 0 94 638 810 899 1197 Size: 94 544 172 89 298 Fragments arranged by size: 544 298 172 94 89 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 163 1197 Size: 163 1034 Tth111I GACn'n_nGTC Cuts at: 0 190 1197 Size: 190 1007 Enzymes that do cut: AccI AflIII AlfI AlwNI AvaI BaeI BanI BanII BbsI BcgI BciVI BglI Bme1580I BmrI BpmI BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BstXI BstYI BtgI BtgZI BtsI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I Hin4I MfeI MmeI MscI MslI MspA1I NcoI NspI PasI PflMI PfoI PmlI PpuMI SacI SfcI SmlI StyI TaqII Tth111I Enzymes that do not cut: AarI AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI AleI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BamHI BbeI BbvCI BclI BfrBI BglII BlpI BmgBI BmtI BplI Bpu10I BsaHI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EagI EciI EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI TstI XbaI XcmI XhoI XmaI XmnI ZraI