(Circular) (Six-base) MAPSORT of: pFN21AB3865.seq Check: 7433 from: 1 to: 5707 pFN21AB3865 5707 bp With 182 enzymes: SgfI * October 20, 2009 10:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2442 2442 Size: 5707 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3517 278 Size: 53 83 186 936 1981 2468 Fragments arranged by size: 2468 1981 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2577 3050 1937 Size: 640 473 4594 Fragments arranged by size: 4594 640 473 Acc65I G'GTAC_C Cuts at: 3028 3028 Size: 5707 AclI AA'CG_TT Cuts at: 1563 3834 4207 1563 Size: 2271 373 3063 Fragments arranged by size: 3063 2271 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3770 5154 5422 1632 Size: 2138 1384 268 1917 Fragments arranged by size: 2138 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5510 AflIII A'CryG_T Cuts at: 1203 2217 4606 5583 1203 Size: 1014 2389 977 1327 Fragments arranged by size: 2389 1327 1014 977 AgeI A'CCGG_T Cuts at: 4538 4538 Size: 5707 AhdI GACnn_n'nnGTC Cuts at: 4436 4436 Size: 5707 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5707 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2929 1184 Size: 1745 3962 AlwNI CAG_nnn'CTG Cuts at: 3450 5022 3450 Size: 1572 4135 ApaLI G'TGCA_C Cuts at: 3764 4920 3764 Size: 1156 4551 ApoI r'AATT_y Cuts at: 1493 3016 3242 3394 5503 1493 Size: 1523 226 152 2109 1697 Fragments arranged by size: 2109 1697 1523 226 152 AseI AT'TA_AT Cuts at: 160 1032 4261 160 Size: 872 3229 1606 Fragments arranged by size: 3229 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5707 AvaI C'yCGr_G Cuts at: 1870 1943 3032 1870 Size: 73 1089 4545 Fragments arranged by size: 4545 1089 73 BamHI G'GATC_C Cuts at: 3037 3404 3037 Size: 367 5340 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2076 2153 2501 3028 4483 Size: 325 217 627 289 77 348 527 1455 Cuts at: 4483 618 Size: 1842 Fragments arranged by size: 1842 1455 627 527 348 325 289 217 77 BanII G_rGCy'C Cuts at: 1391 2616 2901 3026 1391 Size: 1225 285 125 4072 Fragments arranged by size: 4072 1225 285 125 BbeI G_GCGC'C Cuts at: 2157 2157 Size: 5707 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2735 928 Size: 971 836 3900 Fragments arranged by size: 3900 971 836 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3898 3932 1903 Size: 34 50 34 1877 34 3678 Fragments arranged by size: 3678 1877 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3599 4809 3599 Size: 1210 4497 BclI T'GATC_A Cuts at: 1548 1548 Size: 5707 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2568 4318 136 Size: 107 122 71 631 605 896 1750 1525 Fragments arranged by size: 1750 1525 896 631 605 122 107 71 BglII A'GATC_T Cuts at: 5702 5702 Size: 5707 BlpI GC'TnA_GC Cuts at: 3124 3124 Size: 5707 Bme1580I G_kGCm'C Cuts at: 1790 2343 2504 3768 4924 1790 Size: 553 161 1264 1156 2573 Fragments arranged by size: 2573 1264 1156 553 161 BmgBI CAC'GTC Cuts at: 1318 2178 1318 Size: 860 4847 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3438 4396 442 Size: 416 53 83 399 24 2021 958 1753 Fragments arranged by size: 2021 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5707 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2542 2630 2915 4367 1239 Size: 1303 88 285 1452 2579 Fragments arranged by size: 2579 1452 1303 285 88 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3832 4697 4995 3832 Size: 865 298 4544 Fragments arranged by size: 4544 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2534 4370 882 Size: 617 1035 1836 2219 Fragments arranged by size: 2219 1836 1035 617 BsaAI yAC'GTr Cuts at: 493 1204 2218 2266 2338 2440 3498 493 Size: 711 1014 48 72 102 1058 2702 Fragments arranged by size: 2702 1058 1014 711 102 72 48 BsaBI GATnn'nnATC Cuts at: 5701 5701 Size: 5707 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2154 2273 2384 2447 Size: 53 83 186 936 621 119 111 63 Cuts at: 2447 2475 3514 3896 4531 275 Size: 28 1039 382 635 1451 Fragments arranged by size: 1451 1039 936 635 621 382 186 119 111 83 63 53 28 BsaWI w'CCGG_w Cuts at: 4140 4538 4812 4959 5370 4140 Size: 398 274 147 411 4477 Fragments arranged by size: 4477 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5482 Fragments arranged by size: 5482 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5707 BseYI C'CCAG_C Cuts at: 4910 5406 4910 Size: 496 5211 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2158 2315 2318 2420 2899 1163 Size: 573 422 157 3 102 479 3971 Fragments arranged by size: 3971 573 479 422 157 102 3 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2034 3157 3918 4946 1540 Size: 275 186 33 1123 761 1028 2301 Fragments arranged by size: 2301 1123 1028 761 275 186 33 BsiHKAI G_wGCw'C Cuts at: 2087 2616 3768 3853 4924 2087 Size: 529 1152 85 1071 2870 Fragments arranged by size: 2870 1152 1071 529 85 BsmI GAATG_Cn' Cuts at: 3218 3311 3218 Size: 93 5614 BsmBI CGTCTCn'nnnn_ Cuts at: 2008 2438 2466 2008 Size: 430 28 5249 Fragments arranged by size: 5249 430 28 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2087 2343 2504 2616 2901 3026 3768 Size: 399 297 256 161 112 285 125 742 Cuts at: 3768 3853 4924 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 297 285 256 161 125 112 85 BspEI T'CCGG_A Cuts at: 5370 5370 Size: 5707 BspHI T'CATG_A Cuts at: 3596 5326 3596 Size: 1730 3977 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2442 2796 3062 844 Size: 1598 354 266 3489 Fragments arranged by size: 3489 1598 354 266 BsrBI CCG'CTC Cuts at: 1394 1823 3594 1394 Size: 429 1771 3507 Fragments arranged by size: 3507 1771 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4202 4376 65 Size: 1183 2954 174 1396 Fragments arranged by size: 2954 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2414 2748 4351 4538 1700 Size: 110 604 334 1603 187 2869 Fragments arranged by size: 2869 1603 604 334 187 110 BsrGI T'GTAC_A Cuts at: 96 2220 96 Size: 2124 3583 BssHII G'CGCG_C Cuts at: 1925 2396 1925 Size: 471 5236 BssSI C'ACGA_G Cuts at: 3767 4779 3767 Size: 1012 4695 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5707 BstBI TT'CG_AA Cuts at: 2619 4511 2619 Size: 1892 3815 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5707 BstXI CCAn_nnnn'nTGG Cuts at: 2266 2926 2266 Size: 660 5047 BstYI r'GATC_y Cuts at: 1972 2922 3037 3404 3789 3806 5258 5344 5356 Size: 950 115 367 385 17 1452 86 12 Cuts at: 5356 5702 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 950 385 367 346 115 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2237 2495 2693 513 Size: 552 1172 258 198 3527 Fragments arranged by size: 3527 1172 552 258 198 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4968 Fragments arranged by size: 4968 683 56 BtsI GCAGTG_nn' Cuts at: 2131 3223 4017 4037 2131 Size: 1092 794 20 3801 Fragments arranged by size: 3801 1092 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5707 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5672 DraI TTT'AAA Cuts at: 3011 3011 Size: 5707 DraIII CAC_nnn'GTG Cuts at: 2269 2269 Size: 5707 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4714 809 Size: 507 3398 1802 Fragments arranged by size: 3398 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2319 2750 3154 Size: 54 1425 50 275 18 489 431 404 Cuts at: 3154 4043 5672 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 489 431 404 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3154 1537 Size: 275 1342 4090 Fragments arranged by size: 4090 1342 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2006 2616 2621 3637 1353 Size: 653 610 5 1016 3423 Fragments arranged by size: 3423 1016 653 610 5 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4294 4668 4814 387 Size: 1064 138 2705 374 146 1280 Fragments arranged by size: 2705 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2614 2614 Size: 5707 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2542 2630 2915 3770 4367 5154 5422 Size: 393 910 88 285 855 597 787 268 Cuts at: 5422 1239 Size: 1524 Fragments arranged by size: 1524 910 855 787 597 393 285 268 88 EcoO109I rG'GnC_Cy Cuts at: 2689 2689 Size: 5707 EcoRI G'AATT_C Cuts at: 3016 3394 3016 Size: 378 5329 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2998 2998 Size: 5707 FspI TGC'GCA Cuts at: 2207 2630 4213 2207 Size: 423 1583 3701 Fragments arranged by size: 3701 1583 423 FspAI rTGC'GCAy Cuts at: 2207 2207 Size: 5707 HaeII r_GCGC'y Cuts at: 1678 2157 4854 1678 Size: 479 2697 2531 Fragments arranged by size: 2697 2531 479 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2007 2039 2766 2798 2862 Size: 32 135 32 435 32 727 32 64 Cuts at: 2862 2894 4348 4380 4422 4454 1373 Size: 32 1454 32 42 32 2626 Fragments arranged by size: 2626 1454 727 435 135 64 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2785 3051 3297 669 Size: 988 281 847 266 246 3079 Fragments arranged by size: 3079 988 847 281 266 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5707 HpaI GTT'AAC Cuts at: 3297 3297 Size: 5707 KasI G'GCGC_C Cuts at: 2153 2153 Size: 5707 KpnI G_GTAC'C Cuts at: 3032 3032 Size: 5707 MfeI C'AATT_G Cuts at: 3306 3306 Size: 5707 MluI A'CGCG_T Cuts at: 5583 5583 Size: 5707 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2268 4821 5005 1305 Size: 963 2553 184 2007 Fragments arranged by size: 2553 2007 963 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2321 10 Size: 54 1425 343 489 3396 Fragments arranged by size: 3396 1425 489 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2115 2264 3665 4024 4183 518 Size: 648 949 149 1401 359 159 2042 Fragments arranged by size: 2042 1401 949 648 359 159 149 MspA1I CmG'CkG Cuts at: 1606 2389 2497 3123 3800 4948 5193 1606 Size: 783 108 626 677 1148 245 2120 Fragments arranged by size: 2120 1148 783 677 626 245 108 NarI GG'CG_CC Cuts at: 2154 2154 Size: 5707 NcoI C'CATG_G Cuts at: 513 1065 2237 513 Size: 552 1172 3983 Fragments arranged by size: 3983 1172 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5707 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5707 NotI GC'GGCC_GC Cuts at: 3154 3154 Size: 5707 NspI r_CATG'y Cuts at: 1132 1229 3065 4610 1132 Size: 97 1836 1545 2229 Fragments arranged by size: 2229 1836 1545 97 PasI CC'CwG_GG Cuts at: 1348 2163 1348 Size: 815 4892 PciI A'CATG_T Cuts at: 4606 4606 Size: 5707 PflMI CCAn_nnn'nTGG Cuts at: 1108 2356 1108 Size: 1248 4459 PmeI GTTT'AAAC Cuts at: 3011 3011 Size: 5707 PmlI CAC'GTG Cuts at: 2266 2338 2440 2266 Size: 72 102 5533 Fragments arranged by size: 5533 102 72 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3776 3808 5289 5321 3776 Size: 32 1481 32 4162 Fragments arranged by size: 4162 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5707 PsiI TTA'TAA Cuts at: 3277 3277 Size: 5707 PstI C_TGCA'G Cuts at: 830 2805 3059 830 Size: 1975 254 3478 Fragments arranged by size: 3478 1975 254 PvuI CG_AT'CG Cuts at: 2001 2034 2001 Size: 33 5674 SacI G_AGCT'C Cuts at: 2616 2616 Size: 5707 SacII CC_GC'GG Cuts at: 2498 2498 Size: 5707 SalI G'TCGA_C Cuts at: 1936 3049 1936 Size: 1113 4594 SapI GCTCTTCn'nnn_ Cuts at: 2621 2621 Size: 5707 SbfI CC_TGCA'GG Cuts at: 3059 3059 Size: 5707 ScaI AGT'ACT Cuts at: 2171 3955 5667 2171 Size: 1784 1712 2211 Fragments arranged by size: 2211 1784 1712 SfcI C'TryA_G Cuts at: 826 1045 2281 2602 2801 3055 4190 4871 5062 Size: 219 1236 321 199 254 1135 681 191 Cuts at: 5062 826 Size: 1471 Fragments arranged by size: 1471 1236 1135 681 321 254 219 199 191 SfoI GGC'GCC Cuts at: 2155 2155 Size: 5707 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5707 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5707 SmaI CCC'GGG Cuts at: 1872 3034 1872 Size: 1162 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3811 4712 4974 820 Size: 197 926 1868 901 262 1553 Fragments arranged by size: 1868 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3498 493 Size: 3005 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5707 SphI G_CATG'C Cuts at: 1132 3065 1132 Size: 1933 3774 SspI AAT'ATT Cuts at: 5 52 3631 5 Size: 47 3579 2081 Fragments arranged by size: 3579 2081 47 StuI AGG'CCT Cuts at: 1517 2816 1517 Size: 1299 4408 StyI C'CwwG_G Cuts at: 513 1065 2237 2901 3146 513 Size: 552 1172 664 245 3074 Fragments arranged by size: 3074 1172 664 552 245 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2660 3734 3751 3904 4089 4534 325 Size: 1255 1080 1074 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1080 1074 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2169 2220 3953 Size: 274 80 33 51 1443 192 51 1733 Cuts at: 3953 5665 96 Size: 1712 138 Fragments arranged by size: 1733 1712 1443 274 192 138 80 51 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5667 5699 5667 Size: 32 5675 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5707 XbaI T'CTAG_A Cuts at: 3043 3043 Size: 5707 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2263 2263 Size: 5707 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5707 XmaI C'CCGG_G Cuts at: 1870 3032 1870 Size: 1162 4545 XmnI GAAnn'nnTTC Cuts at: 2981 3836 2981 Size: 855 4852 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3515 276 Size: 53 83 186 936 1981 2468 Fragments arranged by size: 2468 1981 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoNI EcoRV FseI NaeI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII SanDI SexAI SfiI SrfI SwaI