################################## # NO CUT ENZYMES ( 125 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AflII AgeI Alw44I ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspI AvrII BalI BamHI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsiEI BsiWI BsmBI Bsp1407I BspDI BspHI BspLU11I BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I CelII ClaI CpoI DraI EaeI EagI EclXI Eco52I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI HincII HindII HindIII HpaI KpnI MamI MfeI MflI MluI MluNI MscI MseI MslI MunI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SalI SbfI ScaI SfcI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI TaqI Tru9I Tsp509I Tth111I TthHB8I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccIII 1193 TCCGGA T^CCGGA AcyI 223 GGCGCC G[GA]^CG[CT]C AflIII 722 ACGTGT A^C[GA][CT]GT AlwI 704 GGATC GGATC(4/5) AocI 34 CCTGAGG CC^T[ACGT]AGG Aor51HI 237 AGCGCT AGC^GCT AviII 341 TGCGCA TGC^GCA BbeI 223 GGCGCC GGCGC^C BbrPI 721 CACGTG CAC^GTG BsaAI 721 CACGTG [CT]AC^GT[GA] BsaHI 223 GGCGCC G[GA]^CG[CT]C BsaI 103 GAGACC GGTCTC(1/5) BsaWI 1193 TCCGGA [AT]^CCGG[AT] BseAI 1193 TCCGGA T^CCGGA BsmI 1203 GCATTC GAATGC(1/-1) BspEI 1193 TCCGGA T^CCGGA BspMI 1023 GCAGGT ACCTGC(4/8) BsrBI 597 CCGCTC CCGCTC(-3/-3) Bsu36I 34 CCTGAGG CC^T[ACGT]AGG DraIII 625 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 550 GACACGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 237 AGCGCT AGC^GCT Eco81I 34 CCTGAGG CC^T[ACGT]AGG FspI 341 TGCGCA TGC^GCA Hin1I 223 GGCGCC G[GA]^CG[CT]C KasI 223 GGCGCC G^GCGCC KspI 89 CCGCGG CCGC^GG MroI 1193 TCCGGA T^CCGGA NaeI 400 GCCGGC GCC^GGC NarI 223 GGCGCC GG^CGCC NgoMIV 400 GCCGGC G^CCGGC PflMI 1387 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 721 CACGTG CAC^GTG PmlI 721 CACGTG CAC^GTG PpuMI 1468 GGGACCC [GA]G^G[AT]CC[CT] PvuII 515 CAGCTG CAG^CTG SacII 89 CCGCGG CCGC^GG SapI 1312 GAAGAGC GCTCTTC(1/4) SexAI 1523 ACCTGGT A^CC[AT]GGT SfoI 223 GGCGCC GGC^GCC Van91I 1387 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1005 CCAGGAAGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AfaI 372 GTAC GT^AC 877 GTAC AhdI 1030 GACCTAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1520 GACACCTGGTC AspEI 1030 GACCTAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1520 GACACCTGGTC AvaI 203 CTCGGG C^[CT]CG[GA]G 1318 CCCGAG BbsI 760 GAAGAC GAAGAC(2/6) 1009 GAAGAC BbvCI 517 GCTGAGG CCTCAGC(-5/-2) 736 GCTGAGG BcnI 273 CCCGG CC^[GC]GG 381 CCGGG BpuAI 760 GAAGAC GAAGAC(2/6) 1009 GAAGAC BsoBI 203 CTCGGG C^[CT]CG[GA]G 1318 CCCGAG BsrDI 953 GCAATG GCAATG(2/0) 1493 GCAATG BsrFI 80 GCCGGT [GA]^CCGG[CT] 400 GCCGGC BsrI 842 ACTGG ACTGG(1/-1) 869 CCAGT Cfr10I 80 GCCGGT [GA]^CCGG[CT] 400 GCCGGC Eam1105I 1030 GACCTAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1520 GACACCTGGTC EarI 478 GAAGAG CTCTTC(1/4) 1312 GAAGAG HaeII 223 GGCGCC [GA]GCGC^[CT] 237 AGCGCT HgaI 139 GACGC GACGC(5/10) 211 GACGC Ksp632I 478 GAAGAG CTCTTC(1/4) 1312 GAAGAG NciI 273 CCCGG CC^[GC]GG 381 CCGGG PleI 169 GACTC GAGTC(4/5) 1012 GACTC RsaI 372 GTAC GT^AC 877 GTAC StuI 38 AGGCCT AGG^CCT 740 AGGCCT TfiI 459 GAATC G^A[AT]TC 973 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 68 enzymes) # ######################################### AccII AciI AluI AlwNI AspHI AvaII BanI BanII BbvI BfaI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EcoNI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI SmlI TseI Tsp45I TspRI