(Linear) (Six-base) MAPSORT of: KIEE6444.seq Check: 4603 from: 1 to: 1530 KIEE6444.orf With 182 enzymes: SgfI * March 16, 2015 11:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1014 1530 Size: 1014 516 AfeI AGC'GCT Cuts at: 0 239 1530 Size: 239 1291 AflIII A'CryG_T Cuts at: 0 722 1530 Size: 722 808 AhdI GACnn_n'nnGTC Cuts at: 0 1035 1525 1530 Size: 1035 490 5 Fragments arranged by size: 1035 490 5 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 367 525 1530 Size: 367 158 1005 Fragments arranged by size: 1005 367 158 AlwNI CAG_nnn'CTG Cuts at: 0 492 660 1295 1393 1530 Size: 492 168 635 98 137 Fragments arranged by size: 635 492 168 137 98 AvaI C'yCGr_G Cuts at: 0 203 1318 1530 Size: 203 1115 212 Fragments arranged by size: 1115 212 203 BanI G'GyrC_C Cuts at: 0 83 223 438 630 1530 Size: 83 140 215 192 900 Fragments arranged by size: 900 215 192 140 83 BanII G_rGCy'C Cuts at: 0 204 274 1319 1530 Size: 204 70 1045 211 Fragments arranged by size: 1045 211 204 70 BbeI G_GCGC'C Cuts at: 0 227 1530 Size: 227 1303 BbsI GAAGACnn'nnnn_ Cuts at: 0 767 1016 1530 Size: 767 249 514 Fragments arranged by size: 767 514 249 BbvCI CC'TCA_GC Cuts at: 0 518 737 1530 Size: 518 219 793 Fragments arranged by size: 793 518 219 Bme1580I G_kGCm'C Cuts at: 0 88 1530 Size: 88 1442 BmgBI CAC'GTC Cuts at: 0 114 1530 Size: 114 1416 BmrI ACTGGGnnnn_n' Cuts at: 0 863 1530 Size: 863 667 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 817 981 1530 Size: 817 164 549 Fragments arranged by size: 817 549 164 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 121 676 1370 1530 Size: 121 555 694 160 Fragments arranged by size: 694 555 160 121 Bpu10I CC'TnA_GC Cuts at: 0 518 737 1530 Size: 518 219 793 Fragments arranged by size: 793 518 219 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 787 951 1264 1530 Size: 787 164 313 266 Fragments arranged by size: 787 313 266 164 BsaI GGTCTCn'nnnn_ Cuts at: 0 97 1530 Size: 97 1433 BsaAI yAC'GTr Cuts at: 0 723 1530 Size: 723 807 BsaHI Gr'CG_yC Cuts at: 0 224 1530 Size: 224 1306 BsaWI w'CCGG_w Cuts at: 0 1193 1530 Size: 1193 337 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 954 1174 1177 1252 1420 1423 1530 Size: 954 220 3 75 168 3 107 Fragments arranged by size: 954 220 168 107 75 3 3 BseYI C'CCAG_C Cuts at: 0 129 528 535 1136 1530 Size: 129 399 7 601 394 Fragments arranged by size: 601 399 394 129 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 229 373 495 1530 Size: 229 144 122 1035 Fragments arranged by size: 1035 229 144 122 BsiHKAI G_wGCw'C Cuts at: 0 338 926 1109 1530 Size: 338 588 183 421 Fragments arranged by size: 588 421 338 183 BsmI GAATG_Cn' Cuts at: 0 1203 1530 Size: 1203 327 Bsp1286I G_dGCh'C Cuts at: 0 88 204 274 338 926 1109 1319 1530 Size: 88 116 70 64 588 183 210 211 Fragments arranged by size: 588 211 210 183 116 88 70 64 BspEI T'CCGG_A Cuts at: 0 1193 1530 Size: 1193 337 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1014 1530 Size: 1014 516 BsrBI CCG'CTC Cuts at: 0 599 1530 Size: 599 931 BsrDI GCAATG_nn' Cuts at: 0 960 1500 1530 Size: 960 540 30 Fragments arranged by size: 960 540 30 BsrFI r'CCGG_y Cuts at: 0 80 400 1530 Size: 80 320 1130 Fragments arranged by size: 1130 320 80 Bsu36I CC'TnA_GG Cuts at: 0 35 1530 Size: 35 1495 BtgI C'CryG_G Cuts at: 0 89 1346 1487 1530 Size: 89 1257 141 43 Fragments arranged by size: 1257 141 89 43 BtsI GCAGTG_nn' Cuts at: 0 356 1530 Size: 356 1174 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 754 789 1530 Size: 754 35 741 Fragments arranged by size: 754 741 35 DraIII CAC_nnn'GTG Cuts at: 0 630 1530 Size: 630 900 DrdI GACnn_nn'nnGTC Cuts at: 0 556 1530 Size: 556 974 EarI CTCTTCn'nnn_ Cuts at: 0 473 1307 1530 Size: 473 834 223 Fragments arranged by size: 834 473 223 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 373 1530 Size: 373 1157 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 121 676 1370 1530 Size: 121 555 694 160 Fragments arranged by size: 694 555 160 121 EcoNI CCTnn'n_nnAGG Cuts at: 0 691 736 747 1530 Size: 691 45 11 783 Fragments arranged by size: 783 691 45 11 EcoO109I rG'GnC_Cy Cuts at: 0 31 489 753 1469 1530 Size: 31 458 264 716 61 Fragments arranged by size: 716 458 264 61 31 FspI TGC'GCA Cuts at: 0 343 1530 Size: 343 1187 HaeII r_GCGC'y Cuts at: 0 227 241 1530 Size: 227 14 1289 Fragments arranged by size: 1289 227 14 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1021 1053 1511 1530 Size: 1021 32 458 19 Fragments arranged by size: 1021 458 32 19 KasI G'GCGC_C Cuts at: 0 223 1530 Size: 223 1307 MspA1I CmG'CkG Cuts at: 0 91 517 929 1530 Size: 91 426 412 601 Fragments arranged by size: 601 426 412 91 NaeI GCC'GGC Cuts at: 0 402 1530 Size: 402 1128 NarI GG'CG_CC Cuts at: 0 224 1530 Size: 224 1306 NgoMIV G'CCGG_C Cuts at: 0 400 1530 Size: 400 1130 PasI CC'CwG_GG Cuts at: 0 12 1530 Size: 12 1518 PflMI CCAn_nnn'nTGG Cuts at: 0 1393 1530 Size: 1393 137 PfoI T'CCnGG_A Cuts at: 0 692 1530 Size: 692 838 PmlI CAC'GTG Cuts at: 0 723 1530 Size: 723 807 PpuMI rG'GwC_Cy Cuts at: 0 1469 1530 Size: 1469 61 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 224 256 1530 Size: 224 32 1274 Fragments arranged by size: 1274 224 32 PvuII CAG'CTG Cuts at: 0 517 1530 Size: 517 1013 SacII CC_GC'GG Cuts at: 0 92 1530 Size: 92 1438 SanDI GG'GwC_CC Cuts at: 0 1469 1530 Size: 1469 61 SapI GCTCTTCn'nnn_ Cuts at: 0 1307 1530 Size: 1307 223 SexAI A'CCwGG_T Cuts at: 0 1523 1530 Size: 1523 7 SfoI GGC'GCC Cuts at: 0 225 1530 Size: 225 1305 SmlI C'TyrA_G Cuts at: 0 802 966 1279 1530 Size: 802 164 313 251 Fragments arranged by size: 802 313 251 164 StuI AGG'CCT Cuts at: 0 40 742 1530 Size: 40 702 788 Fragments arranged by size: 788 702 40 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 724 1048 1530 Size: 724 324 482 Fragments arranged by size: 724 482 324 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1012 1530 Size: 1012 518 Enzymes that do cut: AarI AfeI AflIII AhdI AlfI AlwNI AvaI BanI BanII BbeI BbsI BbvCI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI Bsu36I BtgI BtsI CspCI DraIII DrdI EarI EciI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I KasI MspA1I NaeI NarI NgoMIV PasI PflMI PfoI PmlI PpuMI PsrI PvuII SacII SanDI SapI SexAI SfoI SmlI StuI TaqII XcmI Enzymes that do not cut: AatII AccI Acc65I AclI AcuI AflII AgeI AleI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BcgI BciVI BclI BfrBI BglI BglII BlpI BmtI BsaBI BsaXI BsiEI BsiWI BsmBI BspHI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgZI ClaI DraI EaeI EagI EcoICRI EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI NcoI NdeI NheI NotI NruI NsiI NspI PacI PciI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI RsrII SacI SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StyI SwaI TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI