################################## # NO CUT ENZYMES ( 170 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BsaAI BsaBI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT14I EcoT22I FbaI FseI FspI HgaI HincII HindII HindIII HpaI HphI KpnI Ksp632I KspI MaeI MaeII MaeIII MamI MfeI MluI MluNI MroI MscI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tsp45I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AccII 111 CGCG CG^CG AcsI 172 GAATTC [GA]^AATT[CT] AcyI 20 GGCGCC G[GA]^CG[CT]C AfaI 387 GTAC GT^AC AlwNI 328 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 172 GAATTC [GA]^AATT[CT] AvaII 134 GGACC G^G[AT]CC BamHI 54 GGATCC G^GATCC BanI 20 GGCGCC G^G[CT][GA]CC BanII 77 GGGCTC G[GA]GC[CT]^C BbeI 20 GGCGCC GGCGC^C BbsI 306 GTCTTC GAAGAC(2/6) BbvI 376 GCTGC GCAGC(8/12) BcnI 9 CCCGG CC^[GC]GG BlpI 379 GCTCAGC GC^T[ACGT]AGC BmyI 77 GGGCTC G[AGT]GC[ACT]^C BpmI 59 CTCCAG CTGGAG(16/14) Bpu1102I 379 GCTCAGC GC^T[ACGT]AGC BpuAI 306 GTCTTC GAAGAC(2/6) BsaHI 20 GGCGCC G[GA]^CG[CT]C BsaJI 8 CCCCGG C^C[ACGT][ACGT]GG Bsp1286I 77 GGGCTC G[AGT]GC[ACT]^C BstUI 111 CGCG CG^CG BstYI 54 GGATCC [GA]^GATC[CT] Cac8I 443 GCTGGC GC[ACGT]^[ACGT]GC CelII 379 GCTCAGC GC^T[ACGT]AGC CfoI 21 GCGC GCG^C DpnII 55 GATC ^GATC DraI 157 TTTAAA TTT^AAA DraII 133 GGGACCC [GA]G^G[ACGT]CC[CT] EcoO109I 133 GGGACCC [GA]G^G[ACGT]CC[CT] EcoRI 172 GAATTC G^AATTC HaeII 20 GGCGCC [GA]GCGC^[CT] HaeIII 125 GGCC GG^CC HhaI 21 GCGC GCG^C Hin1I 20 GGCGCC G[GA]^CG[CT]C HinP1I 21 GCGC G^CGC KasI 20 GGCGCC G^GCGCC MboI 55 GATC ^GATC MflI 54 GGATCC [GA]^GATC[CT] MseI 158 TTAA T^TAA MvnI 111 CGCG CG^CG MwoI 21 GCGCCTCATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NarI 20 GGCGCC GG^CGCC NciI 9 CCCGG CC^[GC]GG NdeII 55 GATC ^GATC NlaIII 27 CATG CATG^ PpuMI 133 GGGACCC [GA]G^G[AT]CC[CT] PvuII 382 CAGCTG CAG^CTG RsaI 387 GTAC GT^AC Sau3AI 55 GATC ^GATC SfcI 203 CTACAG C^T[GA][CT]AG SfoI 20 GGCGCC GGC^GCC Tru9I 158 TTAA T^TAA TseI 376 GCTGC G^C[AT]GC Tsp509I 173 AATT ^AATT TspRI 177 CACTG CA[GC]TG[ACGT][ACGT]^ XhoII 54 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AluI 383 AGCT AG^CT 442 AGCT AlwI 55 GATCC GGATC(4/5) 54 GGATC BsmAI 217 GTCTC GTCTC(1/5) 330 GTCTC BsmFI 32 GGGAC GGGAC(10/14) 133 GGGAC BstNI 372 CCAGG CC^[AT]GG 390 CCAGG Cfr13I 124 GGGCC G^G[ACGT]CC 134 GGACC DdeI 221 CTAAG C^T[ACGT]AG 380 CTCAG EcoRII 372 CCAGG ^CC[AT]GG 390 CCAGG FauI 30 GCGGG CCCGC(4/6) 74 GCGGG Fnu4HI 18 GCGGC GC^[ACGT]GC 376 GCTGC HapII 10 CCGG C^CGG 65 CCGG HinfI 68 GAGTC G^A[ACGT]TC 288 GAGTC HpaII 10 CCGG C^CGG 65 CCGG ItaI 18 GCGGC GC^[ACGT]GC 376 GCTGC MboII 181 GAAGA GAAGA(8/7) 307 TCTTC MslI 96 CATAATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 232 CATCCAAATG MspA1I 72 CAGCGG C[AC]G^C[GT]G 382 CAGCTG MspI 10 CCGG C^CGG 65 CCGG MvaI 372 CCAGG CC^[AT]GG 390 CCAGG PleI 68 GAGTC GAGTC(4/5) 288 GAGTC Sau96I 124 GGGCC G^G[ACGT]CC 134 GGACC ######################################### # 3 OR MORE CUT ENZYMES ( 9 enzymes) # ######################################### AciI BsiYI BslI BssKI BstF5I FokI MnlI NlaIV ScrFI