Restriction map for
pFN21AB5715
(Circular) (Six-base) MAPSORT of: pFN21AB5715.seq Check: 8570 from: 1 to: 7066
pFN21AB5715 7066 bp
With 182 enzymes: SgfI *
June 22, 2009 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3096 3133 4142 3096
Size: 37 1009 6020
Fragments arranged by size:
6020 1009 37
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4876 278
Size: 53 83 186 936 3340 2468
Fragments arranged by size:
3340 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2391 4409 1937
Size: 454 2018 4594
Fragments arranged by size:
4594 2018 454
Acc65I G'GTAC_C
Cuts at: 4387 4387
Size: 7066
AclI AA'CG_TT
Cuts at: 1563 5193 5566 1563
Size: 3630 373 3063
Fragments arranged by size:
3630 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2550 3388 3754 5129 6513 6781 1632
Size: 918 838 366 1375 1384 268 1917
Fragments arranged by size:
1917 1384 1375 918 838 366 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6869
AflIII A'CryG_T
Cuts at: 1203 2232 5965 6942 1203
Size: 1029 3733 977 1327
Fragments arranged by size:
3733 1327 1029 977
AgeI A'CCGG_T
Cuts at: 5897 5897
Size: 7066
AhdI GACnn_n'nnGTC
Cuts at: 5795 5795
Size: 7066
AleI CACnn'nnGTG
Cuts at: 1166 2759 3926 1166
Size: 1593 1167 4306
Fragments arranged by size:
4306 1593 1167
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2339 1184
Size: 1155 5911
AlwNI CAG_nnn'CTG
Cuts at: 2377 2678 3763 4300 4809 6381 2377
Size: 301 1085 537 509 1572 3062
Fragments arranged by size:
3062 1572 1085 537 509 301
ApaI G_GGCC'C
Cuts at: 3801 3862 3801
Size: 61 7005
ApaLI G'TGCA_C
Cuts at: 4257 5123 6279 4257
Size: 866 1156 5044
Fragments arranged by size:
5044 1156 866
ApoI r'AATT_y
Cuts at: 1493 4375 4601 4753 6862 1493
Size: 2882 226 152 2109 1697
Fragments arranged by size:
2882 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5620 160
Size: 872 4588 1606
Fragments arranged by size:
4588 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7066
AvaI C'yCGr_G
Cuts at: 1870 1943 2080 2098 2454 3064 3305 3541 4391
Size: 73 137 18 356 610 241 236 850
Cuts at: 4391 1870
Size: 4545
Fragments arranged by size:
4545 850 610 356 241 236 137 73
18
BamHI G'GATC_C
Cuts at: 4240 4396 4763 4240
Size: 156 367 6543
Fragments arranged by size:
6543 367 156
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2867 2900 3044 4060 4154
Size: 325 217 627 1080 33 144 1016 94
Cuts at: 4154 4387 5842 618
Size: 233 1455 1842
Fragments arranged by size:
1842 1455 1080 1016 627 325 233 217
144 94 33
BanII G_rGCy'C
Cuts at: 1391 2040 2246 2922 3741 3801 3834 3862 4227
Size: 649 206 676 819 60 33 28 365
Cuts at: 4227 4282 4385 1391
Size: 55 103 4072
Fragments arranged by size:
4072 819 676 649 365 206 103 60
55 33 28
BbeI G_GCGC'C
Cuts at: 4064 4064
Size: 7066
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6095
BbvCI CC'TCA_GC
Cuts at: 3835 4119 3835
Size: 284 6782
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2530 2564 5257 5291 1903
Size: 34 593 34 2693 34 3678
Fragments arranged by size:
3678 2693 593 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4958 6168 4958
Size: 1210 5856
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7066
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2196 2717 2981
Size: 107 122 71 631 605 524 521 264
Cuts at: 2981 4271 4296 5677 136
Size: 1290 25 1381 1525
Fragments arranged by size:
1525 1381 1290 631 605 524 521 264
122 107 71 25
BglII A'GATC_T
Cuts at: 3984 7061 3984
Size: 3077 3989
BlpI GC'TnA_GC
Cuts at: 2186 2679 4483 2186
Size: 493 1804 4769
Fragments arranged by size:
4769 1804 493
Bme1580I G_kGCm'C
Cuts at: 1790 2592 2872 3047 3801 3862 3945 4055 4261
Size: 802 280 175 754 61 83 110 206
Cuts at: 4261 5127 6283 1790
Size: 866 1156 2573
Fragments arranged by size:
2573 1156 866 802 754 280 206 175
110 83 61
BmgBI CAC'GTC
Cuts at: 1318 2632 1318
Size: 1314 5752
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2848 3672 4092
Size: 416 53 83 399 24 1431 824 420
Cuts at: 4092 4221 4248 4797 5755 442
Size: 129 27 549 958 1753
Fragments arranged by size:
1753 1431 958 824 549 420 416 399
129 83 53 27 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7066
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2941 2941
Size: 7066
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3658 3921 5726 1239
Size: 2419 263 1805 2579
Fragments arranged by size:
2579 2419 1805 263
Bpu10I CC'TnA_GC
Cuts at: 3835 4071 4119 3835
Size: 236 48 6782
Fragments arranged by size:
6782 236 48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5191 6056 6354 5191
Size: 865 298 5903
Fragments arranged by size:
5903 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3338 4207 5729 882
Size: 617 1839 869 1522 2219
Fragments arranged by size:
2219 1839 1522 869 617
BsaAI yAC'GTr
Cuts at: 493 1204 4857 493
Size: 711 3653 2702
Fragments arranged by size:
3653 2702 711
BsaBI GATnn'nnATC
Cuts at: 7060 7060
Size: 7066
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4061 4873 5255 5890
Size: 53 83 186 936 2528 812 382 635
Cuts at: 5890 275
Size: 1451
Fragments arranged by size:
2528 1451 936 812 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2340 5499 5897 6171 6318 6729 2340
Size: 3159 398 274 147 411 2677
Fragments arranged by size:
3159 2677 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2158 2188 2279 2309 1178
Size: 30 165 30 755 30 91 30 5935
Fragments arranged by size:
5935 755 165 91 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2164 2167 3282 3838 3906 4042 1186
Size: 978 3 1115 556 68 136 4210
Fragments arranged by size:
4210 1115 978 556 136 68 3
BseYI C'CCAG_C
Cuts at: 2020 2071 2358 2429 3861 4166 6269 6765 2020
Size: 51 287 71 1432 305 2103 496 2321
Fragments arranged by size:
2321 2103 1432 496 305 287 71 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2494 2521 2570 2719 2913 3673 1163
Size: 573 758 27 49 149 194 760 4556
Fragments arranged by size:
4556 760 758 573 194 149 49 27
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2115 2200 4516 5277 6305 1540
Size: 275 186 114 85 2316 761 1028 2301
Fragments arranged by size:
2316 2301 1028 761 275 186 114 85
BsiHKAI G_wGCw'C
Cuts at: 2040 2328 2685 3240 3396 3605 3741 4261 5127
Size: 288 357 555 156 209 136 520 866
Cuts at: 5127 5212 6283 2040
Size: 85 1071 2823
Fragments arranged by size:
2823 1071 866 555 520 357 288 209
156 136 85
BsmI GAATG_Cn'
Cuts at: 2350 4577 4670 2350
Size: 2227 93 4746
Fragments arranged by size:
4746 2227 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2040 2246 2328 2592 2685 2872 2922
Size: 399 250 206 82 264 93 187 50
Cuts at: 2922 3047 3240 3396 3605 3741 3801 3834 3862
Size: 125 193 156 209 136 60 33 28
Cuts at: 3862 3945 4055 4227 4261 4282 4385 5127 5212
Size: 83 110 172 34 21 103 742 85
Cuts at: 5212 6283 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 264 250 209 206
193 187 172 156 136 125 110 103
93 85 83 82 60 50 34 33
28 21
BspEI T'CCGG_A
Cuts at: 2340 6729 2340
Size: 4389 2677
BspHI T'CATG_A
Cuts at: 3273 4955 6685 3273
Size: 1682 1730 3654
Fragments arranged by size:
3654 1730 1682
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3096 3133 3645 4142 4421 844
Size: 2252 37 512 497 279 3489
Fragments arranged by size:
3489 2252 512 497 279 37
BsrBI CCG'CTC
Cuts at: 1394 1823 2044 2103 2485 2665 4953 1394
Size: 429 221 59 382 180 2288 3507
Fragments arranged by size:
3507 2288 429 382 221 180 59
BsrDI GCAATG_nn'
Cuts at: 65 1248 5561 5735 65
Size: 1183 4313 174 1396
Fragments arranged by size:
4313 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4261 5710 5897 1700
Size: 110 2451 1449 187 2869
Fragments arranged by size:
2869 2451 1449 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7066
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7066
BssSI C'ACGA_G
Cuts at: 2321 3734 5126 6138 2321
Size: 1413 1392 1012 3249
Fragments arranged by size:
3249 1413 1392 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2652 3131 794
Size: 1858 479 4729
Fragments arranged by size:
4729 1858 479
BstBI TT'CG_AA
Cuts at: 5870 5870
Size: 7066
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7066
BstXI CCAn_nnnn'nTGG
Cuts at: 2278 2761 3880 4330 2278
Size: 483 1119 450 5014
Fragments arranged by size:
5014 1119 483 450
BstYI r'GATC_y
Cuts at: 1972 3984 4240 4396 4763 5148 5165 6617 6703
Size: 2012 256 156 367 385 17 1452 86
Cuts at: 6703 6715 7061 1972
Size: 12 346 1977
Fragments arranged by size:
2012 1977 1452 385 367 346 256 156
86 17 12
BstZ17I GTA'TAC
Cuts at: 2392 2392
Size: 7066
Bsu36I CC'TnA_GG
Cuts at: 4236 4236
Size: 7066
BtgI C'CryG_G
Cuts at: 513 1065 2004 3801 4269 513
Size: 552 939 1797 468 3310
Fragments arranged by size:
3310 1797 939 552 468
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2689 3577 487
Size: 683 56 1463 888 3976
Fragments arranged by size:
3976 1463 888 683 56
BtsI GCAGTG_nn'
Cuts at: 2191 2273 2370 4582 5376 5396 2191
Size: 82 97 2212 794 20 3861
Fragments arranged by size:
3861 2212 794 97 82 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7066
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7031
DraI TTT'AAA
Cuts at: 4370 4370
Size: 7066
DraIII CAC_nnn'GTG
Cuts at: 3784 3784
Size: 7066
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6073 809
Size: 507 4757 1802
Fragments arranged by size:
4757 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2197 2280
Size: 54 1425 50 275 18 177 190 83
Cuts at: 2280 2751 4285 4513 5402 7031 8
Size: 471 1534 228 889 1629 43
Fragments arranged by size:
1629 1534 1425 889 471 275 228 190
177 83 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2197 4513 1537
Size: 275 385 2316 4090
Fragments arranged by size:
4090 2316 385 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2672 2747 2810 3692 4019 4028 4996 1353
Size: 1319 75 63 882 327 9 968 3423
Fragments arranged by size:
3423 1319 968 882 327 75 63 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2166 2788 5653 6027 6173 387
Size: 1064 138 577 622 2865 374 146 1280
Fragments arranged by size:
2865 1280 1064 622 577 374 146 138
EcoICRI GAG'CTC
Cuts at: 2038 3739 2038
Size: 1701 5365
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2550 3388 3658 3754 3921 5129 5726
Size: 393 918 838 270 96 167 1208 597
Cuts at: 5726 6513 6781 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1208 918 838 787 597 393 270
268 167 96
EcoNI CCTnn'n_nnAGG
Cuts at: 2444 3652 2444
Size: 1208 5858
EcoO109I rG'GnC_Cy
Cuts at: 2077 2459 4297 4304 2077
Size: 382 1838 7 4839
Fragments arranged by size:
4839 1838 382 7
EcoRI G'AATT_C
Cuts at: 4375 4753 4375
Size: 378 6688
FspI TGC'GCA
Cuts at: 5572 5572
Size: 7066
HaeII r_GCGC'y
Cuts at: 1678 4009 4064 6213 1678
Size: 2331 55 2149 2531
Fragments arranged by size:
2531 2331 2149 55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2158 2190 2279 2311 2868
Size: 32 135 32 586 32 89 32 557
Cuts at: 2868 2900 2924 2956 3140 3172 3520 3552 3833
Size: 32 24 32 184 32 348 32 281
Cuts at: 3833 3865 3868 3900 4340 4372 5707 5739 5781
Size: 32 3 32 440 32 1335 32 42
Cuts at: 5781 5813 1373
Size: 32 2626
Fragments arranged by size:
2626 1335 586 557 440 348 281 184
135 89 42 32 32 32 32 32
32 32 32 32 32 32 32 32
24 3
HincII GTy'rAC
Cuts at: 669 1657 1938 3660 4410 4656 669
Size: 988 281 1722 750 246 3079
Fragments arranged by size:
3079 1722 988 750 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7066
HpaI GTT'AAC
Cuts at: 4656 4656
Size: 7066
KasI G'GCGC_C
Cuts at: 4060 4060
Size: 7066
KpnI G_GTAC'C
Cuts at: 4391 4391
Size: 7066
MfeI C'AATT_G
Cuts at: 4665 4665
Size: 7066
MluI A'CGCG_T
Cuts at: 6942 6942
Size: 7066
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4230 6180 6364 1305
Size: 2925 1950 184 2007
Fragments arranged by size:
2925 2007 1950 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2753 10
Size: 54 1425 343 921 4323
Fragments arranged by size:
4323 1425 921 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2759 3596 3875 3926 5024 5383 5542
Size: 648 1593 837 279 51 1098 359 159
Cuts at: 5542 518
Size: 2042
Fragments arranged by size:
2042 1593 1098 837 648 359 279 159
51
MspA1I CmG'CkG
Cuts at: 1606 2012 2185 2221 2355 3408 3839 4057 4123
Size: 406 173 36 134 1053 431 218 66
Cuts at: 4123 4482 5159 6307 6552 1606
Size: 359 677 1148 245 2120
Fragments arranged by size:
2120 1148 1053 677 431 406 359 245
218 173 134 66 36
NarI GG'CG_CC
Cuts at: 4061 4061
Size: 7066
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5575
Fragments arranged by size:
5575 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7066
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7066
NotI GC'GGCC_GC
Cuts at: 4513 4513
Size: 7066
NspI r_CATG'y
Cuts at: 1132 1229 2398 2695 3778 3869 4424 5969 1132
Size: 97 1169 297 1083 91 555 1545 2229
Fragments arranged by size:
2229 1545 1169 1083 555 297 97 91
PasI CC'CwG_GG
Cuts at: 1348 3040 1348
Size: 1692 5374
PciI A'CATG_T
Cuts at: 5965 5965
Size: 7066
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2619 2860 3966 1108
Size: 1511 241 1106 4208
Fragments arranged by size:
4208 1511 1106 241
PfoI T'CCnGG_A
Cuts at: 3456 3456
Size: 7066
PmeI GTTT'AAAC
Cuts at: 4370 4370
Size: 7066
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5135 5167 6648 6680 5135
Size: 32 1481 32 5521
Fragments arranged by size:
5521 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2077 2077
Size: 7066
PshAI GACnn'nnGTC
Cuts at: 1613 4199 1613
Size: 2586 4480
PsiI TTA'TAA
Cuts at: 4636 4636
Size: 7066
PspOMI G'GGCC_C
Cuts at: 3797 3858 3797
Size: 61 7005
PspXI vC'TCGA_Gb
Cuts at: 2098 2098
Size: 7066
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3182 3214 3182
Size: 32 7034
PstI C_TGCA'G
Cuts at: 830 3747 4002 4153 4418 830
Size: 2917 255 151 265 3478
Fragments arranged by size:
3478 2917 265 255 151
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7066
PvuII CAG'CTG
Cuts at: 3408 3839 4123 3408
Size: 431 284 6351
Fragments arranged by size:
6351 431 284
SacI G_AGCT'C
Cuts at: 2040 3741 2040
Size: 1701 5365
SalI G'TCGA_C
Cuts at: 1936 4408 1936
Size: 2472 4594
SanDI GG'GwC_CC
Cuts at: 2077 2077
Size: 7066
SapI GCTCTTCn'nnn_
Cuts at: 2672 2747 3692 2672
Size: 75 945 6046
Fragments arranged by size:
6046 945 75
SbfI CC_TGCA'GG
Cuts at: 4418 4418
Size: 7066
ScaI AGT'ACT
Cuts at: 5314 7026 5314
Size: 1712 5354
SexAI A'CCwGG_T
Cuts at: 3095 3647 3095
Size: 552 6514
SfcI C'TryA_G
Cuts at: 826 1045 3743 3998 4149 4414 5549 6230 6421
Size: 219 2698 255 151 265 1135 681 191
Cuts at: 6421 826
Size: 1471
Fragments arranged by size:
2698 1471 1135 681 265 255 219 191
151
SfiI GGCCn_nnn'nGGCC
Cuts at: 2717 2717
Size: 7066
SfoI GGC'GCC
Cuts at: 4062 4062
Size: 7066
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7066
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7066
SmaI CCC'GGG
Cuts at: 1872 2082 3307 4393 1872
Size: 210 1225 1086 4545
Fragments arranged by size:
4545 1225 1086 210
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2098 5170 6071 6333 820
Size: 197 926 155 3072 901 262 1553
Fragments arranged by size:
3072 1553 926 901 262 197 155
SnaBI TAC'GTA
Cuts at: 493 4857 493
Size: 4364 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7066
SphI G_CATG'C
Cuts at: 1132 3778 4424 1132
Size: 2646 646 3774
Fragments arranged by size:
3774 2646 646
SspI AAT'ATT
Cuts at: 5 52 4990 5
Size: 47 4938 2081
Fragments arranged by size:
4938 2081 47
StuI AGG'CCT
Cuts at: 1517 3719 1517
Size: 2202 4864
StyI C'CwwG_G
Cuts at: 513 1065 2004 2592 3618 3714 4505 513
Size: 552 939 588 1026 96 791 3074
Fragments arranged by size:
3074 1026 939 791 588 552 96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5093 5110 5263 5448 5893 325
Size: 1255 3513 17 153 185 445 1498
Fragments arranged by size:
3513 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2967 5312 7024
Size: 274 80 33 51 1443 990 2345 1712
Cuts at: 7024 96
Size: 138
Fragments arranged by size:
2345 1712 1443 990 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3770 3802 7026 7058 3770
Size: 32 3224 32 3778
Fragments arranged by size:
3778 3224 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2938 1316
Size: 1622 5444
XbaI T'CTAG_A
Cuts at: 4402 4402
Size: 7066
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2861 3355 3967 2861
Size: 494 612 5960
Fragments arranged by size:
5960 612 494
XhoI C'TCGA_G
Cuts at: 1943 2098 1943
Size: 155 6911
XmaI C'CCGG_G
Cuts at: 1870 2080 3305 4391 1870
Size: 210 1225 1086 4545
Fragments arranged by size:
4545 1225 1086 210
XmnI GAAnn'nnTTC
Cuts at: 5195 5195
Size: 7066
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4874 276
Size: 53 83 186 936 3340 2468
Fragments arranged by size:
3340 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI
EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PmlI RsrII SacII SrfI SwaI