################################## # NO CUT ENZYMES ( 115 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AvrII BbeI BbrPI BbsI BciVI BfrI BglI BglII BlnI BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BspDI BspEI BspHI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoT22I FseI HaeII Hin1I HindIII HpaI KasI KpnI Ksp632I KspI MamI MfeI MluI MroI MspA1I MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI SwaI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AccII 1386 CGCG CG^CG AcsI 994 GAATTC [GA]^AATT[CT] AflIII 1045 ACATGT A^C[GA][CT]GT AlwNI 1114 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 994 GAATTC [GA]^AATT[CT] Asp700I 1636 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 1229 TGCGCA TGC^GCA BalI 1248 TGGCCA TGG^CCA BamHI 1413 GGATCC G^GATCC BanI 1510 GGTGCC G^G[CT][GA]CC BanII 1174 GAGCTC G[GA]GC[CT]^C BcgI 1266 GCAGGACACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 872 TGATCA T^GATCA BlpI 319 GCTAAGC GC^T[ACGT]AGC Bpu1102I 319 GCTAAGC GC^T[ACGT]AGC BseRI 187 GAGGAG GAGGAG(10/8) BsgI 936 GTGCAG GTGCAG(16/14) Bsp1407I 1048 TGTACA T^GTACA BspLU11I 1045 ACATGT A^CATGT BspMI 1490 GCAGGT ACCTGC(4/8) BsrGI 1048 TGTACA T^GTACA BstUI 1386 CGCG CG^CG CelII 319 GCTAAGC GC^T[ACGT]AGC CfoI 1230 GCGC GCG^C DsaI 1682 CCATGG C^C[GA][CT]GG EaeI 1248 TGGCCA [CT]^GGCC[GA] EcoRI 994 GAATTC G^AATTC EcoRV 667 GATATC GAT^ATC FbaI 872 TGATCA T^GATCA FspI 1229 TGCGCA TGC^GCA HgaI 988 GACGC GACGC(5/10) HhaI 1230 GCGC GCG^C HinP1I 1230 GCGC G^CGC HincII 1422 GTCAAC GT[CT]^[GA]AC HindII 1422 GTCAAC GT[CT]^[GA]AC MboII 1836 GAAGA GAAGA(8/7) MluNI 1248 TGGCCA TGG^CCA MscI 1248 TGGCCA TGG^CCA MslI 1626 CACCTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 1386 CGCG CG^CG NcoI 1682 CCATGG C^CATGG NspI 1045 ACATGT [GA]CATG^[CT] PaeR7I 1396 CTCGAG C^TCGAG SacI 1174 GAGCTC GAGCT^C SbfI 1540 CCTGCAGG CCTGCA^GG ScaI 827 AGTACT AGT^ACT SexAI 611 ACCAGGT A^CC[AT]GGT SmaI 1754 CCCGGG CCC^GGG Sse8387I 1540 CCTGCAGG CCTGCA^GG SspBI 1048 TGTACA T^GTACA StuI 483 AGGCCT AGG^CCT Tsp45I 599 GTGAC ^GT[GC]AC XbaI 1362 TCTAGA T^CTAGA XcmI 1673 CCAAAAAAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1396 CTCGAG C^TCGAG XmaCI 1754 CCCGGG C^CCGGG XmaI 1754 CCCGGG C^CCGGG XmnI 1636 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AspHI 1174 GAGCTC G[AT]GC[AT]^C 1393 GTGCTC AvaI 1396 CTCGAG C^[CT]CG[GA]G 1754 CCCGGG BbvCI 310 GCTGAGG CCTCAGC(-5/-2) 715 GCTGAGG BmyI 1174 GAGCTC G[AGT]GC[ACT]^C 1393 GTGCTC BsaI 1106 GAGACC GGTCTC(1/5) 145 GGTCTC BsiHKAI 1174 GAGCTC G[AT]GC[AT]^C 1393 GTGCTC BsoBI 1396 CTCGAG C^[CT]CG[GA]G 1754 CCCGGG Bsp1286I 1174 GAGCTC G[AGT]GC[ACT]^C 1393 GTGCTC DraIII 67 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG 956 CACATGGTG EcoT14I 1343 CCAAGG C^C[AT][AT]GG 1682 CCATGG HphI 1074 GGTGA GGTGA(8/7) 1625 TCACC MaeII 476 ACGT A^CGT 707 ACGT MaeIII 218 GTTAC ^GT[ACGT]AC 599 GTGAC PflMI 612 CCAGGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1793 CCAAAGCATGG SmlI 1396 CTCGAG C^T[CT][GA]AG 1605 CTTGAG SspI 435 AATATT AAT^ATT 457 AATATT StyI 1343 CCAAGG C^C[AT][AT]GG 1682 CCATGG TaqI 1397 TCGA T^CGA 1737 TCGA TthHB8I 1397 TCGA T^CGA 1737 TCGA Van91I 612 CCAGGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1793 CCAAAGCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 65 enzymes) # ######################################### AciI AfaI AluI AlwI AspI AvaII BbvI BcnI BfaI BpmI BsaJI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI MaeI MboI MflI MnlI MseI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflFI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TfiI Tru9I TseI Tsp509I TspRI Tth111I XhoII