(Linear) (Six-base) MAPSORT of: KIEE6430.seq Check: 4888 from: 1 to: 1842 KIEE6430.orf With 182 enzymes: SgfI * March 16, 2015 11:31 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1096 1842 Size: 1096 746 AflIII A'CryG_T Cuts at: 0 1045 1842 Size: 1045 797 AleI CACnn'nnGTG Cuts at: 0 1630 1842 Size: 1630 212 AlwNI CAG_nnn'CTG Cuts at: 0 1119 1842 Size: 1119 723 ApoI r'AATT_y Cuts at: 0 994 1842 Size: 994 848 AvaI C'yCGr_G Cuts at: 0 1396 1754 1842 Size: 1396 358 88 Fragments arranged by size: 1396 358 88 BamHI G'GATC_C Cuts at: 0 1413 1842 Size: 1413 429 BanI G'GyrC_C Cuts at: 0 1510 1842 Size: 1510 332 BanII G_rGCy'C Cuts at: 0 1178 1842 Size: 1178 664 BbvCI CC'TCA_GC Cuts at: 0 311 716 1842 Size: 311 405 1126 Fragments arranged by size: 1126 405 311 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1255 1289 1842 Size: 1255 34 553 Fragments arranged by size: 1255 553 34 BclI T'GATC_A Cuts at: 0 872 1842 Size: 872 970 BlpI GC'TnA_GC Cuts at: 0 320 1842 Size: 320 1522 BmgBI CAC'GTC Cuts at: 0 708 1842 Size: 708 1134 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 205 745 1141 1192 1842 Size: 205 540 396 51 650 Fragments arranged by size: 650 540 396 205 51 Bpu10I CC'TnA_GC Cuts at: 0 311 563 716 1842 Size: 311 252 153 1126 Fragments arranged by size: 1126 311 252 153 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1626 1842 Size: 1626 216 BsaI GGTCTCn'nnnn_ Cuts at: 0 151 1100 1842 Size: 151 949 742 Fragments arranged by size: 949 742 151 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 184 214 811 841 1842 Size: 184 30 597 30 1001 Fragments arranged by size: 1001 597 184 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 202 1842 Size: 202 1640 BseYI C'CCAG_C Cuts at: 0 505 788 1842 Size: 505 283 1054 Fragments arranged by size: 1054 505 283 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 957 1842 Size: 957 885 BsiHKAI G_wGCw'C Cuts at: 0 1178 1397 1842 Size: 1178 219 445 Fragments arranged by size: 1178 445 219 BsmI GAATG_Cn' Cuts at: 0 396 1206 1219 1553 1842 Size: 396 810 13 334 289 Fragments arranged by size: 810 396 334 289 13 BsmBI CGTCTCn'nnnn_ Cuts at: 0 467 909 980 1451 1842 Size: 467 442 71 471 391 Fragments arranged by size: 471 467 442 391 71 Bsp1286I G_dGCh'C Cuts at: 0 1178 1397 1842 Size: 1178 219 445 Fragments arranged by size: 1178 445 219 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1481 1842 Size: 1481 361 BsrGI T'GTAC_A Cuts at: 0 1048 1842 Size: 1048 794 BstYI r'GATC_y Cuts at: 0 756 1413 1572 1842 Size: 756 657 159 270 Fragments arranged by size: 756 657 270 159 BtgI C'CryG_G Cuts at: 0 1682 1842 Size: 1682 160 BtsI GCAGTG_nn' Cuts at: 0 1485 1842 Size: 1485 357 DraIII CAC_nnn'GTG Cuts at: 0 72 961 1842 Size: 72 889 881 Fragments arranged by size: 889 881 72 EaeI y'GGCC_r Cuts at: 0 1248 1842 Size: 1248 594 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 289 1386 1842 Size: 289 1097 456 Fragments arranged by size: 1097 456 289 EcoICRI GAG'CTC Cuts at: 0 1176 1842 Size: 1176 666 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 205 745 1096 1141 1192 1842 Size: 205 540 351 45 51 650 Fragments arranged by size: 650 540 351 205 51 45 EcoO109I rG'GnC_Cy Cuts at: 0 1407 1493 1536 1806 1826 1842 Size: 1407 86 43 270 20 16 Fragments arranged by size: 1407 270 86 43 20 16 EcoRI G'AATT_C Cuts at: 0 994 1842 Size: 994 848 EcoRV GAT'ATC Cuts at: 0 669 1842 Size: 669 1173 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 846 1842 Size: 846 996 FspI TGC'GCA Cuts at: 0 1231 1842 Size: 1231 611 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1405 1437 1711 1743 1842 Size: 1405 32 274 32 99 Fragments arranged by size: 1405 274 99 32 32 HincII GTy'rAC Cuts at: 0 1424 1842 Size: 1424 418 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 81 1842 Size: 81 1761 MscI TGG'CCA Cuts at: 0 1250 1842 Size: 1250 592 MslI CAynn'nnrTG Cuts at: 0 1630 1842 Size: 1630 212 NcoI C'CATG_G Cuts at: 0 1682 1842 Size: 1682 160 NspI r_CATG'y Cuts at: 0 1049 1842 Size: 1049 793 PciI A'CATG_T Cuts at: 0 1045 1842 Size: 1045 797 PflMI CCAn_nnn'nTGG Cuts at: 0 618 1799 1842 Size: 618 1181 43 Fragments arranged by size: 1181 618 43 PfoI T'CCnGG_A Cuts at: 0 1409 1495 1842 Size: 1409 86 347 Fragments arranged by size: 1409 347 86 PpuMI rG'GwC_Cy Cuts at: 0 1407 1493 1536 1806 1826 1842 Size: 1407 86 43 270 20 16 Fragments arranged by size: 1407 270 86 43 20 16 PspXI vC'TCGA_Gb Cuts at: 0 1396 1842 Size: 1396 446 PstI C_TGCA'G Cuts at: 0 912 1492 1545 1842 Size: 912 580 53 297 Fragments arranged by size: 912 580 297 53 SacI G_AGCT'C Cuts at: 0 1178 1842 Size: 1178 664 SanDI GG'GwC_CC Cuts at: 0 1536 1842 Size: 1536 306 SbfI CC_TGCA'GG Cuts at: 0 1545 1842 Size: 1545 297 ScaI AGT'ACT Cuts at: 0 829 1842 Size: 829 1013 SexAI A'CCwGG_T Cuts at: 0 611 1842 Size: 611 1231 SfcI C'TryA_G Cuts at: 0 908 1488 1541 1842 Size: 908 580 53 301 Fragments arranged by size: 908 580 301 53 SmaI CCC'GGG Cuts at: 0 1756 1842 Size: 1756 86 SmlI C'TyrA_G Cuts at: 0 1396 1605 1842 Size: 1396 209 237 Fragments arranged by size: 1396 237 209 SspI AAT'ATT Cuts at: 0 437 459 1842 Size: 437 22 1383 Fragments arranged by size: 1383 437 22 StuI AGG'CCT Cuts at: 0 485 1842 Size: 485 1357 StyI C'CwwG_G Cuts at: 0 1343 1682 1842 Size: 1343 339 160 Fragments arranged by size: 1343 339 160 TatI w'GTAC_w Cuts at: 0 827 894 1048 1842 Size: 827 67 154 794 Fragments arranged by size: 827 794 154 67 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 116 148 748 780 1477 1509 1842 Size: 116 32 600 32 697 32 333 Fragments arranged by size: 697 600 333 116 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 238 604 706 1842 Size: 238 366 102 1136 Fragments arranged by size: 1136 366 238 102 XbaI T'CTAG_A Cuts at: 0 1362 1842 Size: 1362 480 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1680 1842 Size: 1680 162 XhoI C'TCGA_G Cuts at: 0 1396 1842 Size: 1396 446 XmaI C'CCGG_G Cuts at: 0 1754 1842 Size: 1754 88 XmnI GAAnn'nnTTC Cuts at: 0 1640 1842 Size: 1640 202 Enzymes that do cut: AcuI AflIII AleI AlwNI ApoI AvaI BamHI BanI BanII BbvCI BcgI BclI BlpI BmgBI BpmI Bpu10I BpuEI BsaI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrGI BstYI BtgI BtsI DraIII EaeI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI Hin4I HincII MmeI MscI MslI NcoI NspI PciI PflMI PfoI PpuMI PspXI PstI SacI SanDI SbfI ScaI SexAI SfcI SmaI SmlI SspI StuI StyI TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BbeI BbsI BciVI BfrBI BglI BglII Bme1580I BmrI BmtI BplI BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BspEI BspHI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EagI EarI EcoNI FseI FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI PvuI PvuII RsrII SacII SalI SapI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SwaI TaqII ZraI