################################## # NO CUT ENZYMES ( 183 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcyI AfaI AflII AflIII AgeI AhdI AlwI AocI Aor51HI AscI AseI AsnI Asp718I AspEI AspI AvaI AvaII AviII AvrII BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BseRI BsgI BsiEI BsiWI BsmFI BsoBI Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DpnII DraI DraIII DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HphI KasI KpnI Ksp632I KspI MaeI MamI MboI MfeI MflI MluI MseI MslI MspA1I MunI MvnI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### AccIII 521 TCCGGA T^CCGGA AciI 507 GCGG CCGC(-3/-1) Alw44I 497 GTGCAC G^TGCAC ApaLI 497 GTGCAC G^TGCAC Asp700I 405 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 422 TGGCCA TGG^CCA BcnI 269 CCCGG CC^[GC]GG BglI 217 GCCTTCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaWI 521 TCCGGA [AT]^CCGG[AT] BseAI 521 TCCGGA T^CCGGA BsmBI 300 CGTCTC CGTCTC(1/5) BsmI 529 GCATTC GAATGC(1/-1) BspEI 521 TCCGGA T^CCGGA BsrI 461 CCAGT ACTGG(1/-1) BssSI 261 CACGAG CACGAG(-5/-1) DrdI 399 GACTCTGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 422 TGGCCA [CT]^GGCC[GA] EcoRI 160 GAATTC G^AATTC EcoT14I 570 CCTTGG C^C[AT][AT]GG HinfI 399 GACTC G^A[ACGT]TC MboII 588 TCTTC GAAGA(8/7) MluNI 422 TGGCCA TGG^CCA MroI 521 TCCGGA T^CCGGA MscI 422 TGGCCA TGG^CCA NciI 269 CCCGG CC^[GC]GG PleI 399 GACTC GAGTC(4/5) StyI 570 CCTTGG C^C[AT][AT]GG Tsp45I 447 GTCAC ^GT[GC]AC TspRI 495 CAGTG CA[GC]TG[ACGT][ACGT]^ XmnI 405 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AlwNI 13 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG 202 CAGAAACTG ApaI 146 GGGCCC GGGCC^C 172 GGGCCC AspHI 97 GTGCTC G[AT]GC[AT]^C 497 GTGCAC BanII 146 GGGCCC G[GA]GC[CT]^C 172 GGGCCC BpmI 129 CTGGAG CTGGAG(16/14) 340 CTGGAG BsiHKAI 97 GTGCTC G[AT]GC[AT]^C 497 GTGCAC BsmAI 491 GAGAC GTCTC(1/5) 301 GTCTC BstF5I 306 GGATG GGATG(2/0) 599 GGATG DdeI 248 CTCAG C^T[ACGT]AG 311 CTGAG DraII 145 GGGGCCC [GA]G^G[ACGT]CC[CT] 172 GGGCCCT EcoO109I 145 GGGGCCC [GA]G^G[ACGT]CC[CT] 172 GGGCCCT FokI 306 GGATG GGATG(9/13) 599 GGATG HapII 270 CCGG C^CGG 522 CCGG HpaII 270 CCGG C^CGG 522 CCGG MaeII 299 ACGT A^CGT 545 ACGT MaeIII 320 GTTAC ^GT[ACGT]AC 447 GTCAC MspI 270 CCGG C^CGG 522 CCGG PstI 277 CTGCAG CTGCA^G 292 CTGCAG SfaNI 307 GATGC GCATC(5/9) 600 GATGC SfcI 277 CTGCAG C^T[GA][CT]AG 292 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 25 enzymes) # ######################################### AcsI AluI ApoI BbvI BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstNI Cac8I Cfr13I EcoRII Fnu4HI HaeIII ItaI MnlI MvaI MwoI NlaIV Sau96I ScrFI TseI Tsp509I