################################## # NO CUT ENZYMES ( 169 enzymes) # ################################## Acc65I AccII AciI AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaJI BseRI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HhaI HinP1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MamI MfeI MluI MluNI MscI MseI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tru9I Tsp509I Tth111I TthHB8I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AatII 346 GACGTC GACGT^C AccI 148 GTCTAC GT^[AC][GT]AC AccIII 425 TCCGGA T^CCGGA AcyI 346 GACGTC G[GA]^CG[CT]C AlwI 262 GATCC GGATC(4/5) AlwNI 226 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 279 GAGCAC G[AT]GC[AT]^C AvaII 123 GGTCC G^G[AT]CC BanII 134 GAGCCC G[GA]GC[CT]^C BbsI 23 GTCTTC GAAGAC(2/6) BcnI 509 CCCGG CC^[GC]GG BfaI 253 CTAG C^TAG BpmI 160 CTCCAG CTGGAG(16/14) BpuAI 23 GTCTTC GAAGAC(2/6) BsaHI 346 GACGTC G[GA]^CG[CT]C BsaI 327 GAGACC GGTCTC(1/5) BsaWI 425 TCCGGA [AT]^CCGG[AT] BseAI 425 TCCGGA T^CCGGA BsgI 495 GTGCAG GTGCAG(16/14) BsiHKAI 279 GAGCAC G[AT]GC[AT]^C BspEI 425 TCCGGA T^CCGGA BspHI 387 TCATGA T^CATGA BsrDI 526 CATTGC GCAATG(2/0) BssSI 131 CACGAG CACGAG(-5/-1) BstNI 185 CCTGG CC^[AT]GG BstYI 261 AGATCC [GA]^GATC[CT] DdeI 323 CTGAG C^T[ACGT]AG DpnII 262 GATC ^GATC DraII 122 AGGTCCC [GA]G^G[ACGT]CC[CT] DrdI 115 GACATTCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO109I 122 AGGTCCC [GA]G^G[ACGT]CC[CT] EcoRII 185 CCTGG ^CC[AT]GG HgaI 315 GACGC GACGC(5/10) Hin1I 346 GACGTC G[GA]^CG[CT]C HphI 482 TCACC GGTGA(8/7) MaeI 253 CTAG C^TAG MaeIII 256 GTGAC ^GT[ACGT]AC MboI 262 GATC ^GATC MflI 261 AGATCC [GA]^GATC[CT] MroI 425 TCCGGA T^CCGGA MslI 388 CATGAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvaI 185 CCTGG CC^[AT]GG MwoI 136 GCCCCAGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NciI 509 CCCGG CC^[GC]GG NdeII 262 GATC ^GATC NlaIV 123 GGTCCC GG[ACGT]^[ACGT]CC PflMI 225 CCAGCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 100 GACTC GAGTC(4/5) PpuMI 122 AGGTCCC [GA]G^G[AT]CC[CT] RcaI 387 TCATGA T^CATGA Sau3AI 262 GATC ^GATC SmlI 370 CTCAAG C^T[CT][GA]AG Tsp45I 256 GTGAC ^GT[GC]AC Van91I 225 CCAGCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 175 CCAACCCCAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 261 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### BmyI 134 GAGCCC G[AGT]GC[ACT]^C 279 GAGCAC BsmAI 268 GAGAC GTCTC(1/5) 327 GAGAC BsmFI 124 GTCCC GGGAC(10/14) 507 GTCCC Bsp1286I 134 GAGCCC G[AGT]GC[ACT]^C 279 GAGCAC BssKI 185 CCTGG ^CC[ACGT]GG 509 CCCGG Cac8I 189 GCCTGC GC[ACGT]^[ACGT]GC 497 GCAGGC Cfr13I 123 GGTCC G^G[ACGT]CC 247 GGGCC MspA1I 449 CAGCTG C[AC]G^C[GT]G 502 CAGCTG NlaIII 111 CATG CATG^ 388 CATG PvuII 449 CAGCTG CAG^CTG 502 CAGCTG Sau96I 123 GGTCC G^G[ACGT]CC 247 GGGCC ScrFI 185 CCTGG CC^[ACGT]GG 509 CCCGG SfaNI 142 GATGC GCATC(5/9) 470 GCATC TfiI 45 GATTC G^A[AT]TC 522 GAATC TspRI 300 CACTG CA[GC]TG[ACGT][ACGT]^ 486 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 18 enzymes) # ######################################### AluI BbvI BsiYI BslI BsrI BstF5I Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI MaeII MboII MnlI MspI TseI