(Circular) (Six-base) MAPSORT of: pFN21AE5207.seq Check: 389 from: 1 to: 5239 pFN21AE5207 5239 bp With 182 enzymes: SgfI * January 30, 2014 17:14 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2355 3049 278 Size: 53 83 186 936 819 694 2468 Fragments arranged by size: 2468 936 819 694 186 83 53 AccI GT'mk_AC Cuts at: 1937 2154 2582 1937 Size: 217 428 4594 Fragments arranged by size: 4594 428 217 Acc65I G'GTAC_C Cuts at: 2560 2560 Size: 5239 AclI AA'CG_TT Cuts at: 1563 3366 3739 1563 Size: 1803 373 3063 Fragments arranged by size: 3063 1803 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2082 3302 4686 4954 1632 Size: 450 1220 1384 268 1917 Fragments arranged by size: 1917 1384 1220 450 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5042 AflIII A'CryG_T Cuts at: 1203 4138 5115 1203 Size: 2935 977 1327 Fragments arranged by size: 2935 1327 977 AgeI A'CCGG_T Cuts at: 4070 4070 Size: 5239 AhdI GACnn_n'nnGTC Cuts at: 3968 3968 Size: 5239 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5239 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5239 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2412 2444 2412 Size: 32 5207 AlwNI CAG_nnn'CTG Cuts at: 2236 2982 4554 2236 Size: 746 1572 2921 Fragments arranged by size: 2921 1572 746 ApaLI G'TGCA_C Cuts at: 3296 4452 3296 Size: 1156 4083 ApoI r'AATT_y Cuts at: 1493 2548 2774 2926 5035 1493 Size: 1055 226 152 2109 1697 Fragments arranged by size: 2109 1697 1055 226 152 AseI AT'TA_AT Cuts at: 160 1032 3793 160 Size: 872 2761 1606 Fragments arranged by size: 2761 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5239 AvaI C'yCGr_G Cuts at: 1870 1943 2564 1870 Size: 73 621 4545 Fragments arranged by size: 4545 621 73 BamHI G'GATC_C Cuts at: 2569 2936 2569 Size: 367 4872 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2560 4015 618 Size: 325 217 627 773 1455 1842 Fragments arranged by size: 1842 1455 773 627 325 217 BanII G_rGCy'C Cuts at: 1391 2143 2558 1391 Size: 752 415 4072 Fragments arranged by size: 4072 752 415 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2021 928 Size: 971 122 4146 Fragments arranged by size: 4146 971 122 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3430 3464 1903 Size: 34 1493 34 3678 Fragments arranged by size: 3678 1493 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3131 4341 3131 Size: 1210 4029 BclI T'GATC_A Cuts at: 1548 1548 Size: 5239 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3850 136 Size: 107 122 71 631 605 2178 1525 Fragments arranged by size: 2178 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5234 5234 Size: 5239 BlpI GC'TnA_GC Cuts at: 2656 2656 Size: 5239 Bme1580I G_kGCm'C Cuts at: 1790 3300 4456 1790 Size: 1510 1156 2573 Fragments arranged by size: 2573 1510 1156 BmgBI CAC'GTC Cuts at: 1318 2088 1318 Size: 770 4469 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2970 3928 442 Size: 416 53 83 399 24 1553 958 1753 Fragments arranged by size: 1753 1553 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5239 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2150 3899 1239 Size: 911 1749 2579 Fragments arranged by size: 2579 1749 911 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2360 3364 4229 4527 2360 Size: 1004 865 298 3072 Fragments arranged by size: 3072 1004 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2326 3902 882 Size: 617 827 1576 2219 Fragments arranged by size: 2219 1576 827 617 BsaAI yAC'GTr Cuts at: 493 1204 3030 493 Size: 711 1826 2702 Fragments arranged by size: 2702 1826 711 BsaBI GATnn'nnATC Cuts at: 5233 5233 Size: 5239 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2352 3046 3428 4063 Size: 53 83 186 936 819 694 382 635 Cuts at: 4063 275 Size: 1451 Fragments arranged by size: 1451 936 819 694 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2430 3672 4070 4344 4491 4902 2430 Size: 1242 398 274 147 411 2767 Fragments arranged by size: 2767 1242 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2412 2442 1178 Size: 30 165 30 1009 30 3975 Fragments arranged by size: 3975 1009 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5239 BseYI C'CCAG_C Cuts at: 2229 4442 4938 2229 Size: 2213 496 2530 Fragments arranged by size: 2530 2213 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2521 1163 Size: 573 785 3881 Fragments arranged by size: 3881 785 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2689 3450 4478 1540 Size: 275 186 688 761 1028 2301 Fragments arranged by size: 2301 1028 761 688 275 186 BsiHKAI G_wGCw'C Cuts at: 2288 3300 3385 4456 2288 Size: 1012 85 1071 3071 Fragments arranged by size: 3071 1071 1012 85 BsmI GAATG_Cn' Cuts at: 2750 2843 2750 Size: 93 5146 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2143 2288 2558 3300 3385 4456 1391 Size: 399 353 145 270 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 353 270 145 85 BspEI T'CCGG_A Cuts at: 2430 4902 2430 Size: 2472 2767 BspHI T'CATG_A Cuts at: 2392 3128 4858 2392 Size: 736 1730 2773 Fragments arranged by size: 2773 1730 736 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2594 844 Size: 1750 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3126 1394 Size: 429 1303 3507 Fragments arranged by size: 3507 1303 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2530 3734 3908 65 Size: 1183 1282 1204 174 1396 Fragments arranged by size: 1396 1282 1204 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3883 4070 1700 Size: 110 2073 187 2869 Fragments arranged by size: 2869 2073 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5239 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5239 BssSI C'ACGA_G Cuts at: 2136 3299 4311 2136 Size: 1163 1012 3064 Fragments arranged by size: 3064 1163 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5239 BstBI TT'CG_AA Cuts at: 4043 4043 Size: 5239 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5239 BstYI r'GATC_y Cuts at: 1972 2266 2569 2936 3321 3338 4790 4876 4888 Size: 294 303 367 385 17 1452 86 12 Cuts at: 4888 5234 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 385 367 346 303 294 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4687 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4500 Fragments arranged by size: 4500 683 56 BtsI GCAGTG_nn' Cuts at: 2755 3549 3569 2755 Size: 794 20 4425 Fragments arranged by size: 4425 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5239 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5204 DraI TTT'AAA Cuts at: 2543 2543 Size: 5239 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2126 4246 809 Size: 507 810 2120 1802 Fragments arranged by size: 2120 1802 810 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2686 3575 5204 Size: 54 1425 50 275 18 856 889 1629 Cuts at: 5204 8 Size: 43 Fragments arranged by size: 1629 1425 889 856 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2686 1537 Size: 275 874 4090 Fragments arranged by size: 4090 874 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3169 1353 Size: 1816 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3826 4200 4346 387 Size: 1064 138 2237 374 146 1280 Fragments arranged by size: 2237 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2082 2150 3302 3899 4686 4954 1239 Size: 393 450 68 1152 597 787 268 1524 Fragments arranged by size: 1524 1152 787 597 450 393 268 68 EcoO109I rG'GnC_Cy Cuts at: 2128 2128 Size: 5239 EcoRI G'AATT_C Cuts at: 2548 2926 2548 Size: 378 4861 FspI TGC'GCA Cuts at: 3745 3745 Size: 5239 HaeII r_GCGC'y Cuts at: 1678 4386 1678 Size: 2708 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2072 2104 2321 2353 3880 Size: 32 135 32 500 32 217 32 1527 Cuts at: 3880 3912 3954 3986 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1527 500 217 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2583 2829 669 Size: 988 281 645 246 3079 Fragments arranged by size: 3079 988 645 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5239 HpaI GTT'AAC Cuts at: 2829 2829 Size: 5239 KpnI G_GTAC'C Cuts at: 2564 2564 Size: 5239 MfeI C'AATT_G Cuts at: 2838 2838 Size: 5239 MluI A'CGCG_T Cuts at: 5115 5115 Size: 5239 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2204 4353 4537 1305 Size: 899 2149 184 2007 Fragments arranged by size: 2149 2007 899 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3417 Fragments arranged by size: 3417 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2397 3197 3556 3715 518 Size: 648 1231 800 359 159 2042 Fragments arranged by size: 2042 1231 800 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2456 2509 2655 3332 4480 4725 1606 Size: 850 53 146 677 1148 245 2120 Fragments arranged by size: 2120 1148 850 677 245 146 53 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4687 NdeI CA'TA_TG Cuts at: 387 387 Size: 5239 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5239 NotI GC'GGCC_GC Cuts at: 2686 2686 Size: 5239 NspI r_CATG'y Cuts at: 1132 1229 2597 4142 1132 Size: 97 1368 1545 2229 Fragments arranged by size: 2229 1545 1368 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5239 PciI A'CATG_T Cuts at: 4138 4138 Size: 5239 PflMI CCAn_nnn'nTGG Cuts at: 1108 2236 1108 Size: 1128 4111 PmeI GTTT'AAAC Cuts at: 2543 2543 Size: 5239 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2412 2444 3308 3340 4821 4853 2412 Size: 32 864 32 1481 32 2798 Fragments arranged by size: 2798 1481 864 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2128 2128 Size: 5239 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5239 PsiI TTA'TAA Cuts at: 2809 2809 Size: 5239 PstI C_TGCA'G Cuts at: 830 2591 830 Size: 1761 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5239 PvuII CAG'CTG Cuts at: 2456 2509 2456 Size: 53 5186 SalI G'TCGA_C Cuts at: 1936 2581 1936 Size: 645 4594 SbfI CC_TGCA'GG Cuts at: 2591 2591 Size: 5239 ScaI AGT'ACT Cuts at: 3487 5199 3487 Size: 1712 3527 SfcI C'TryA_G Cuts at: 826 1045 2587 3722 4403 4594 826 Size: 219 1542 1135 681 191 1471 Fragments arranged by size: 1542 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5239 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5239 SmaI CCC'GGG Cuts at: 1872 2566 1872 Size: 694 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2375 3343 4244 4506 820 Size: 197 926 432 968 901 262 1553 Fragments arranged by size: 1553 968 926 901 432 262 197 SnaBI TAC'GTA Cuts at: 493 3030 493 Size: 2537 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5239 SphI G_CATG'C Cuts at: 1132 2597 1132 Size: 1465 3774 SspI AAT'ATT Cuts at: 5 52 3163 5 Size: 47 3111 2081 Fragments arranged by size: 3111 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5239 StyI C'CwwG_G Cuts at: 513 1065 2678 513 Size: 552 1613 3074 Fragments arranged by size: 3074 1613 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3266 3283 3436 3621 4066 325 Size: 1255 1686 17 153 185 445 1498 Fragments arranged by size: 1686 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3485 5197 96 Size: 274 80 33 51 1443 1508 1712 138 Fragments arranged by size: 1712 1508 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5199 5231 5199 Size: 32 5207 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5239 XbaI T'CTAG_A Cuts at: 2575 2575 Size: 5239 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2187 2187 Size: 5239 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5239 XmaI C'CCGG_G Cuts at: 1870 2564 1870 Size: 694 4545 XmnI GAAnn'nnTTC Cuts at: 3368 3368 Size: 5239 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2353 3047 276 Size: 53 83 186 936 819 694 2468 Fragments arranged by size: 2468 936 819 694 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI