(Linear) (Six-base) MAPSORT of: KIEE5207.seq  Check: 3196  from: 1  to: 534



KIEE5207.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:50  ..

AatII G_ACGT'C

Cuts at:      0     350     534 
   Size:        350     184
 
AccI GT'mk_AC

Cuts at:      0     149     534 
   Size:        149     385
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      77     534 
   Size:         77     457
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     407     439     534 
   Size:        407      32      95

  Fragments arranged by size: 

                 407      95      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     231     534 
   Size:        231     303
 
BanII G_rGCy'C

Cuts at:      0     138     534 
   Size:        138     396
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      16     534 
   Size:         16     518
 
BmgBI CAC'GTC

Cuts at:      0      83     534 
   Size:         83     451
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     145     534 
   Size:        145     389
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     355     534 
   Size:        355     179
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     321     534 
   Size:        321     213
 
BsaHI Gr'CG_yC

Cuts at:      0     347     534 
   Size:        347     187
 
BsaWI w'CCGG_w

Cuts at:      0     425     534 
   Size:        425     109
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     407     437     534 
   Size:        407      30      97

  Fragments arranged by size: 

                 407      97      30
 
BseYI C'CCAG_C

Cuts at:      0     224     534 
   Size:        224     310
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     516     534 
   Size:        516      18
 
BsiHKAI G_wGCw'C

Cuts at:      0     283     534 
   Size:        283     251
 
Bsp1286I G_dGCh'C

Cuts at:      0     138     283     534 
   Size:        138     145     251

  Fragments arranged by size: 

                 251     145     138
 
BspEI T'CCGG_A

Cuts at:      0     425     534 
   Size:        425     109
 
BspHI T'CATG_A

Cuts at:      0     387     534 
   Size:        387     147
 
BsrDI GCAATG_nn'

Cuts at:      0     525     534 
   Size:        525       9
 
BssSI C'ACGA_G

Cuts at:      0     131     534 
   Size:        131     403
 
BstYI r'GATC_y

Cuts at:      0     261     534 
   Size:        261     273
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     121     534 
   Size:        121     413
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      77     145     534 
   Size:         77      68     389

  Fragments arranged by size: 

                 389      77      68
 
EcoO109I rG'GnC_Cy

Cuts at:      0     123     534 
   Size:        123     411
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      67      99     316     348     534 
   Size:         67      32     217      32     186

  Fragments arranged by size: 

                 217     186      67      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     199     534 
   Size:        199     335
 
MslI CAynn'nnrTG

Cuts at:      0     392     534 
   Size:        392     142
 
MspA1I CmG'CkG

Cuts at:      0     451     504     534 
   Size:        451      53      30

  Fragments arranged by size: 

                 451      53      30
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     231     534 
   Size:        231     303
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     407     439     534 
   Size:        407      32      95

  Fragments arranged by size: 

                 407      95      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     123     534 
   Size:        123     411
 
PvuII CAG'CTG

Cuts at:      0     451     504     534 
   Size:        451      53      30

  Fragments arranged by size: 

                 451      53      30
 
SmlI C'TyrA_G

Cuts at:      0     370     534 
   Size:        370     164
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     182     534 
   Size:        182     352
 
ZraI GAC'GTC

Cuts at:      0     348     534 
   Size:        348     186
 

 Enzymes that do cut:

    AatII     AccI     AcuI     AloI    AlwNI    BanII     BbsI    BmgBI
     BpmI    BpuEI     BsaI    BsaHI    BsaWI    BsaXI    BseYI     BsgI
  BsiHKAI Bsp1286I    BspEI    BspHI    BsrDI    BssSI    BstYI     DrdI
  Eco57MI EcoO109I    Hin4I     MmeI     MslI   MspA1I    PflMI     PpiI
    PpuMI    PvuII     SmlI     XcmI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I     BmrI
     BmtI     BplI   Bpu10I    BsaAI    BsaBI    BseRI    BsiEI    BsiWI
     BsmI    BsmBI    BspMI    BsrBI    BsrFI    BsrGI   BssHII   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     EaeI     EagI     EarI     EciI
  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI
     PmlI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI