################################## # NO CUT ENZYMES ( 111 enzymes) # ################################## AatII AccI AccIII AclI AflII AflIII AgeI AocI Aor51HI ApaI AscI AseI AsnI Asp700I AspI AviII AvrII BbvCI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaI BseAI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI MamI MluI MroI MseI NdeI NheI NotI NruI NsiI NspV PacI PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tth111I XbaI XcmI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### Acc65I 818 GGTACC G^GTACC AcsI 793 AAATTT [GA]^AATT[CT] AhdI 460 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1205 GTGCAC G^TGCAC ApaLI 1205 GTGCAC G^TGCAC ApoI 793 AAATTT [GA]^AATT[CT] Asp718I 818 GGTACC G^GTACC AspEI 460 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 1025 TGGCCA TGG^CCA BamHI 147 GGATCC G^GATCC BbeI 1305 GGCGCC GGCGC^C BbrPI 1174 CACGTG CAC^GTG BfaI 130 CTAG C^TAG BglI 229 GCCCTGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaWI 773 TCCGGT [AT]^CCGG[AT] BseRI 1227 CTCCTC GAGGAG(10/8) BsrFI 316 GCCGGC [GA]^CCGG[CT] BssSI 427 CACGAG CACGAG(-5/-1) Cfr10I 316 GCCGGC [GA]^CCGG[CT] DraIII 855 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 460 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoT14I 308 CCATGG C^C[AT][AT]GG HgaI 375 GCGTC GACGC(5/10) KasI 1305 GGCGCC G^GCGCC KpnI 818 GGTACC GGTAC^C KspI 1238 CCGCGG CCGC^GG MaeI 130 CTAG C^TAG MfeI 138 CAATTG C^AATTG MluNI 1025 TGGCCA TGG^CCA MscI 1025 TGGCCA TGG^CCA MunI 138 CAATTG C^AATTG NaeI 316 GCCGGC GCC^GGC NarI 1305 GGCGCC GG^CGCC NcoI 308 CCATGG C^CATGG NgoMIV 316 GCCGGC G^CCGGC NspI 617 ACATGC [GA]CATG^[CT] PaeR7I 1381 CTCGAG C^TCGAG PmaCI 1174 CACGTG CAC^GTG PmlI 1174 CACGTG CAC^GTG PpuMI 302 AGGTCCC [GA]G^G[AT]CC[CT] SacI 979 GAGCTC GAGCT^C SacII 1238 CCGCGG CCGC^GG SfoI 1305 GGCGCC GGC^GCC SmlI 1381 CTCGAG C^T[CT][GA]AG StyI 308 CCATGG C^C[AT][AT]GG XhoI 1381 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AcyI 374 GGCGTC G[GA]^CG[CT]C 1305 GGCGCC BanII 54 GAGCCC G[GA]GC[CT]^C 979 GAGCTC BcnI 406 CCGGG CC^[GC]GG 1328 CCGGG BsaAI 1174 CACGTG [CT]AC^GT[GA] 1294 TACGTG BsaHI 374 GGCGTC G[GA]^CG[CT]C 1305 GGCGCC BsiEI 1325 CGGCCG CG[GA][CT]^CG 1340 CGGCCG BsmFI 1092 GGGAC GGGAC(10/14) 304 GTCCC BspMI 108 ACCTGC ACCTGC(4/8) 1038 GCAGGT BsrBI 754 CCGCTC CCGCTC(-3/-3) 441 GAGCGG BstAPI 632 GCACCAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1005 GCAGGCCCTGC DraII 302 AGGTCCC [GA]G^G[ACGT]CC[CT] 1007 AGGCCCT EagI 1325 CGGCCG C^GGCCG 1340 CGGCCG EarI 682 CTCTTC CTCTTC(1/4) 535 GAAGAG EclXI 1325 CGGCCG C^GGCCG 1340 CGGCCG Eco52I 1325 CGGCCG C^GGCCG 1340 CGGCCG EcoO109I 302 AGGTCCC [GA]G^G[ACGT]CC[CT] 1007 AGGCCCT HaeII 660 GGCGCT [GA]GCGC^[CT] 1305 GGCGCC Hin1I 374 GGCGTC G[GA]^CG[CT]C 1305 GGCGCC Ksp632I 682 CTCTTC CTCTTC(1/4) 535 GAAGAG MslI 387 CACCATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 390 CATTGTGGTG NciI 406 CCGGG CC^[GC]GG 1328 CCGGG PflMI 882 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1170 CCATCACGTGG PleI 460 GACTC GAGTC(4/5) 841 GACTC PstI 1198 CTGCAG CTGCA^G 1351 CTGCAG TaqI 839 TCGA T^CGA 1382 TCGA TspRI 41 CAGTG CA[GC]TG[ACGT][ACGT]^ 1310 CAGTG TthHB8I 839 TCGA T^CGA 1382 TCGA Van91I 882 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1170 CCATCACGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 73 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BanI BbsI BbvI BmyI BpmI BpuAI BsaJI BsgI BsiHKAI BsiYI BslI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TfiI TseI Tsp45I Tsp509I XhoII