################################## # NO CUT ENZYMES ( 124 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AgeI AhdI AlwNI AocI Aor51HI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BbeI BbsI BbvCI BcgI BciVI BclI BfrI BglII BlnI BpuAI BsaBI BsaHI BsaI BsaWI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrFI BsrGI BssHII Bst1107I BstBI BstUI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRV FbaI FseI FspI HgaI Hin1I HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MseI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SspBI SspI SwaI Tru9I Tth111I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccI 531 GTCTAC GT^[AC][GT]AC AcsI 502 GAATTC [GA]^AATT[CT] AflIII 180 ACACGT A^C[GA][CT]GT ApoI 502 GAATTC [GA]^AATT[CT] AvaI 188 CCCGGG C^[CT]CG[GA]G BamHI 362 GGATCC G^GATCC BbrPI 22 CACGTG CAC^GTG BfaI 649 CTAG C^TAG BlpI 1131 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1131 GCTGAGC GC^T[ACGT]AGC BsgI 1111 GTGCAG GTGCAG(16/14) BsiEI 1142 CGACCG CG[GA][CT]^CG BsmAI 703 GTCTC GTCTC(1/5) BsmFI 134 GGGAC GGGAC(10/14) BsoBI 188 CCCGGG C^[CT]CG[GA]G BspMI 34 GCAGGT ACCTGC(4/8) BsrBI 463 GAGCGG CCGCTC(-3/-3) BssSI 847 CACGAG CACGAG(-5/-1) BstAPI 50 GCAATGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1096 GGTGACC G^GT[ACGT]ACC BstPI 1096 GGTGACC G^GT[ACGT]ACC CelII 1131 GCTGAGC GC^T[ACGT]AGC EarI 256 CTCTTC CTCTTC(1/4) EcoO65I 1096 GGTGACC G^GT[ACGT]ACC EcoRI 502 GAATTC G^AATTC EcoT22I 9 ATGCAT ATGCA^T FauI 1166 CCCGC CCCGC(4/6) HaeII 659 AGCGCC [GA]GCGC^[CT] Ksp632I 256 CTCTTC CTCTTC(1/4) MaeI 649 CTAG C^TAG MspA1I 853 CAGCTG C[AC]G^C[GT]G NsiI 9 ATGCAT ATGCA^T NspI 1127 GCATGC [GA]CATG^[CT] PmaCI 22 CACGTG CAC^GTG PmlI 22 CACGTG CAC^GTG PstI 830 CTGCAG CTGCA^G PvuII 853 CAGCTG CAG^CTG SacI 64 GAGCTC GAGCT^C SapI 255 GCTCTTC GCTCTTC(1/4) SexAI 773 ACCTGGT A^CC[AT]GGT SmaI 188 CCCGGG CCC^GGG SphI 1127 GCATGC GCATG^C StuI 1157 AGGCCT AGG^CCT TfiI 87 GATTC G^A[AT]TC XcmI 414 CCACAGCTTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 188 CCCGGG C^CCGGG XmaI 188 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### Alw44I 384 GTGCAC G^TGCAC 1213 GTGCAC ApaI 737 GGGCCC GGGCC^C 1163 GGGCCC ApaLI 384 GTGCAC G^TGCAC 1213 GTGCAC AvaII 750 GGACC G^G[AT]CC 1173 GGACC BalI 806 TGGCCA TGG^CCA 1103 TGGCCA BsaAI 22 CACGTG [CT]AC^GT[GA] 181 CACGTA BsmI 7 GAATGC GAATGC(1/-1) 1019 GCATTC BsrDI 993 CATTGC GCAATG(2/0) 50 GCAATG BstXI 414 CCACAGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 896 CCAACCAGATGG DraII 736 GGGGCCC [GA]G^G[ACGT]CC[CT] 737 GGGCCCT EaeI 806 TGGCCA [CT]^GGCC[GA] 1103 TGGCCA EcoNI 1101 CCTGGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1281 CCTGGAGAAGG EcoO109I 736 GGGGCCC [GA]G^G[ACGT]CC[CT] 737 GGGCCCT EcoT14I 974 CCAAGG C^C[AT][AT]GG 1106 CCAAGG HapII 189 CCGG C^CGG 921 CCGG HincII 475 GTTGAC GT[CT]^[GA]AC 982 GTCAAC HindII 475 GTTGAC GT[CT]^[GA]AC 982 GTCAAC HpaII 189 CCGG C^CGG 921 CCGG MaeII 23 ACGT A^CGT 182 ACGT MluNI 806 TGGCCA TGG^CCA 1103 TGGCCA MscI 806 TGGCCA TGG^CCA 1103 TGGCCA MslI 1080 CACTGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1216 CACTGGTATG MspI 189 CCGG C^CGG 921 CCGG PleI 139 GACTC GAGTC(4/5) 1312 GACTC SfcI 533 CTACAG C^T[GA][CT]AG 830 CTGCAG StyI 974 CCAAGG C^C[AT][AT]GG 1106 CCAAGG Tsp509I 503 AATT ^AATT 699 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 60 enzymes) # ######################################### AciI AfaI AluI AlwI AspHI BanI BanII BbvI BcnI BglI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DsaI EcoRII Fnu4HI FokI HaeIII HhaI HinP1I HinfI HphI ItaI MaeIII MboI MboII MflI MnlI MvaI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI TaqI TseI Tsp45I TspRI TthHB8I XhoII