(Circular) (Six-base) MAPSORT of: pFN21AE1423.seq Check: 6453 from: 1 to: 6049 pFN21AE1423 6049 bp With 182 enzymes: SgfI * August 16, 2010 14:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2030 2030 Size: 6049 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3859 278 Size: 53 83 186 936 2323 2468 Fragments arranged by size: 2468 2323 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2537 3392 1937 Size: 600 855 4594 Fragments arranged by size: 4594 855 600 Acc65I G'GTAC_C Cuts at: 3370 3370 Size: 6049 AclI AA'CG_TT Cuts at: 1563 4176 4549 1563 Size: 2613 373 3063 Fragments arranged by size: 3063 2613 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2384 4112 5496 5764 1632 Size: 752 1728 1384 268 1917 Fragments arranged by size: 1917 1728 1384 752 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5852 AflIII A'CryG_T Cuts at: 1203 2185 4948 5925 1203 Size: 982 2763 977 1327 Fragments arranged by size: 2763 1327 982 977 AgeI A'CCGG_T Cuts at: 4880 4880 Size: 6049 AhdI GACnn_n'nnGTC Cuts at: 4778 4778 Size: 6049 AleI CACnn'nnGTG Cuts at: 1166 3089 1166 Size: 1923 4126 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6049 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3170 3202 3239 3271 3170 Size: 32 37 32 5948 Fragments arranged by size: 5948 37 32 32 AlwNI CAG_nnn'CTG Cuts at: 3792 5364 3792 Size: 1572 4477 ApaI G_GGCC'C Cuts at: 2746 3172 2746 Size: 426 5623 ApaLI G'TGCA_C Cuts at: 2389 3218 4106 5262 2389 Size: 829 888 1156 3176 Fragments arranged by size: 3176 1156 888 829 ApoI r'AATT_y Cuts at: 1493 2507 3358 3584 3736 5845 1493 Size: 1014 851 226 152 2109 1697 Fragments arranged by size: 2109 1697 1014 851 226 152 AseI AT'TA_AT Cuts at: 160 1032 4603 160 Size: 872 3571 1606 Fragments arranged by size: 3571 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6049 AvaI C'yCGr_G Cuts at: 1870 1943 2193 3374 1870 Size: 73 250 1181 4545 Fragments arranged by size: 4545 1181 250 73 BamHI G'GATC_C Cuts at: 2367 3379 3746 2367 Size: 1012 367 4670 Fragments arranged by size: 4670 1012 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2782 2974 3042 3091 3370 Size: 325 217 627 995 192 68 49 279 Cuts at: 3370 4825 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 995 627 325 279 217 192 68 49 BanII G_rGCy'C Cuts at: 1391 2073 2556 2746 3172 3368 1391 Size: 682 483 190 426 196 4072 Fragments arranged by size: 4072 682 483 426 196 190 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5078 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4240 4274 1903 Size: 34 50 34 2219 34 3678 Fragments arranged by size: 3678 2219 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3941 5151 3941 Size: 1210 4839 BclI T'GATC_A Cuts at: 1548 1548 Size: 6049 BfrBI ATG'CAT Cuts at: 2016 2016 Size: 6049 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2104 3051 3209 Size: 107 122 71 631 605 432 947 158 Cuts at: 3209 4660 136 Size: 1451 1525 Fragments arranged by size: 1525 1451 947 631 605 432 158 122 107 71 BglII A'GATC_T Cuts at: 6044 6044 Size: 6049 BlpI GC'TnA_GC Cuts at: 3137 3466 3137 Size: 329 5720 Bme1580I G_kGCm'C Cuts at: 1790 2393 2438 2746 2787 2979 3047 3172 3222 Size: 603 45 308 41 192 68 125 50 Cuts at: 3222 4110 5266 1790 Size: 888 1156 2573 Fragments arranged by size: 2573 1156 888 603 308 192 125 68 50 45 41 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6049 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2109 2295 2348 Size: 416 53 83 399 24 692 186 53 Cuts at: 2348 3780 4738 442 Size: 1432 958 1753 Fragments arranged by size: 1753 1432 958 692 416 399 186 83 53 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6049 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2491 2491 Size: 6049 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2234 2609 3308 4709 1239 Size: 995 375 699 1401 2579 Fragments arranged by size: 2579 1401 995 699 375 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4174 5039 5337 4174 Size: 865 298 4886 Fragments arranged by size: 4886 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4712 882 Size: 617 3213 2219 Fragments arranged by size: 3213 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2029 2188 3840 493 Size: 711 825 159 1652 2702 Fragments arranged by size: 2702 1652 825 711 159 BsaBI GATnn'nnATC Cuts at: 6043 6043 Size: 6049 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3856 4238 4873 275 Size: 53 83 186 936 2323 382 635 1451 Fragments arranged by size: 2323 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4482 4880 5154 5301 5712 4482 Size: 398 274 147 411 4819 Fragments arranged by size: 4819 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2958 2988 3239 3269 3333 Size: 30 165 30 1555 30 251 30 64 Cuts at: 3333 3363 1178 Size: 30 3864 Fragments arranged by size: 3864 1555 251 165 64 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2701 3260 3317 1186 Size: 1515 559 57 3918 Fragments arranged by size: 3918 1515 559 57 BseYI C'CCAG_C Cuts at: 2064 2521 5252 5748 2064 Size: 457 2731 496 2365 Fragments arranged by size: 2731 2365 496 457 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3137 1163 Size: 573 1401 4075 Fragments arranged by size: 4075 1401 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3150 3499 4260 5288 1540 Size: 275 186 1149 349 761 1028 2301 Fragments arranged by size: 2301 1149 1028 761 349 275 186 BsiHKAI G_wGCw'C Cuts at: 2073 2393 2595 3222 4110 4195 5266 2073 Size: 320 202 627 888 85 1071 2856 Fragments arranged by size: 2856 1071 888 627 320 202 85 BsmI GAATG_Cn' Cuts at: 2018 3024 3560 3653 2018 Size: 1006 536 93 4414 Fragments arranged by size: 4414 1006 536 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2073 2393 2438 2556 2595 2746 2787 Size: 399 283 320 45 118 39 151 41 Cuts at: 2787 2979 3047 3172 3222 3368 4110 4195 5266 Size: 192 68 125 50 146 742 85 1071 Cuts at: 5266 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 320 283 192 151 146 125 118 85 68 50 45 41 39 BspEI T'CCGG_A Cuts at: 5712 5712 Size: 6049 BspHI T'CATG_A Cuts at: 3938 5668 3938 Size: 1730 4319 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2030 3404 844 Size: 1186 1374 3489 Fragments arranged by size: 3489 1374 1186 BsrBI CCG'CTC Cuts at: 1394 1823 2470 3936 1394 Size: 429 647 1466 3507 Fragments arranged by size: 3507 1466 647 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2062 2997 4544 4718 65 Size: 1183 814 935 1547 174 1396 Fragments arranged by size: 1547 1396 1183 935 814 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4693 4880 1700 Size: 110 2883 187 2869 Fragments arranged by size: 2883 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6049 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6049 BssSI C'ACGA_G Cuts at: 2852 4109 5121 2852 Size: 1257 1012 3780 Fragments arranged by size: 3780 1257 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2061 794 Size: 1267 4782 BstBI TT'CG_AA Cuts at: 4853 4853 Size: 6049 BstEII G'GTnAC_C Cuts at: 1184 3101 1184 Size: 1917 4132 BstXI CCAn_nnnn'nTGG Cuts at: 2426 2908 2426 Size: 482 5567 BstYI r'GATC_y Cuts at: 1972 2209 2235 2367 3379 3746 4131 4148 5600 Size: 237 26 132 1012 367 385 17 1452 Cuts at: 5600 5686 5698 6044 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1012 385 367 346 237 132 86 26 17 12 BtgI C'CryG_G Cuts at: 513 1065 2317 2544 2962 513 Size: 552 1252 227 418 3600 Fragments arranged by size: 3600 1252 552 418 227 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3139 487 Size: 683 56 1913 3397 Fragments arranged by size: 3397 1913 683 56 BtsI GCAGTG_nn' Cuts at: 3565 4359 4379 3565 Size: 794 20 5235 Fragments arranged by size: 5235 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6049 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6014 DraI TTT'AAA Cuts at: 3353 3353 Size: 6049 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5056 809 Size: 507 3740 1802 Fragments arranged by size: 3740 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2811 3108 3496 Size: 54 1425 50 275 18 981 297 388 Cuts at: 3496 4385 6014 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 981 889 388 297 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3496 1537 Size: 275 1684 4090 Fragments arranged by size: 4090 1684 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2267 3979 1353 Size: 914 1712 3423 Fragments arranged by size: 3423 1712 914 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4636 5010 5156 387 Size: 1064 138 3047 374 146 1280 Fragments arranged by size: 3047 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2071 2071 Size: 6049 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2234 2384 2609 3308 4112 4709 5496 Size: 393 602 150 225 699 804 597 787 Cuts at: 5496 5764 1239 Size: 268 1524 Fragments arranged by size: 1524 804 787 699 602 597 393 268 225 150 EcoNI CCTnn'n_nnAGG Cuts at: 3110 3290 3110 Size: 180 5869 EcoO109I rG'GnC_Cy Cuts at: 2742 2743 2742 Size: 1 6048 EcoRI G'AATT_C Cuts at: 2507 3358 3736 2507 Size: 851 378 4820 Fragments arranged by size: 4820 851 378 FspI TGC'GCA Cuts at: 4555 4555 Size: 6049 HaeII r_GCGC'y Cuts at: 1678 2668 5196 1678 Size: 990 2528 2531 Fragments arranged by size: 2531 2528 990 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2846 2878 4690 4722 4764 Size: 32 135 32 1274 32 1812 32 42 Cuts at: 4764 4796 1373 Size: 32 2626 Fragments arranged by size: 2626 1812 1274 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2482 2989 3393 3639 669 Size: 988 281 544 507 404 246 3079 Fragments arranged by size: 3079 988 544 507 404 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6049 HpaI GTT'AAC Cuts at: 3639 3639 Size: 6049 KpnI G_GTAC'C Cuts at: 3374 3374 Size: 6049 MfeI C'AATT_G Cuts at: 3648 3648 Size: 6049 MluI A'CGCG_T Cuts at: 5925 5925 Size: 6049 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5163 5347 1305 Size: 3858 184 2007 Fragments arranged by size: 3858 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2813 3110 10 Size: 54 1425 343 981 297 2949 Fragments arranged by size: 2949 1425 981 343 297 54 MslI CAynn'nnrTG Cuts at: 518 1166 3089 3225 4007 4366 4525 518 Size: 648 1923 136 782 359 159 2042 Fragments arranged by size: 2042 1923 782 648 359 159 136 MspA1I CmG'CkG Cuts at: 1606 2860 3465 4142 5290 5535 1606 Size: 1254 605 677 1148 245 2120 Fragments arranged by size: 2120 1254 1148 677 605 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5497 NdeI CA'TA_TG Cuts at: 387 387 Size: 6049 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6049 NotI GC'GGCC_GC Cuts at: 3496 3496 Size: 6049 NsiI A_TGCA'T Cuts at: 2018 2018 Size: 6049 NspI r_CATG'y Cuts at: 1132 1229 3136 3407 4952 1132 Size: 97 1907 271 1545 2229 Fragments arranged by size: 2229 1907 1545 271 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6049 PciI A'CATG_T Cuts at: 4948 4948 Size: 6049 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6049 PmeI GTTT'AAAC Cuts at: 3353 3353 Size: 6049 PmlI CAC'GTG Cuts at: 2029 2029 Size: 6049 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3239 3271 4118 4150 5631 5663 3239 Size: 32 847 32 1481 32 3625 Fragments arranged by size: 3625 1481 847 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6049 PsiI TTA'TAA Cuts at: 3619 3619 Size: 6049 PspOMI G'GGCC_C Cuts at: 2742 3168 2742 Size: 426 5623 PstI C_TGCA'G Cuts at: 830 2839 3401 830 Size: 2009 562 3478 Fragments arranged by size: 3478 2009 562 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6049 PvuII CAG'CTG Cuts at: 2860 2860 Size: 6049 SacI G_AGCT'C Cuts at: 2073 2073 Size: 6049 SalI G'TCGA_C Cuts at: 1936 3391 1936 Size: 1455 4594 SapI GCTCTTCn'nnn_ Cuts at: 2267 2267 Size: 6049 SbfI CC_TGCA'GG Cuts at: 3401 3401 Size: 6049 ScaI AGT'ACT Cuts at: 4297 6009 4297 Size: 1712 4337 SexAI A'CCwGG_T Cuts at: 2778 2778 Size: 6049 SfcI C'TryA_G Cuts at: 826 1045 2538 2835 3397 4532 5213 5404 826 Size: 219 1493 297 562 1135 681 191 1471 Fragments arranged by size: 1493 1471 1135 681 562 297 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6049 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6049 SmaI CCC'GGG Cuts at: 1872 2195 3376 1872 Size: 323 1181 4545 Fragments arranged by size: 4545 1181 323 SmlI C'TyrA_G Cuts at: 820 1017 1943 4153 5054 5316 820 Size: 197 926 2210 901 262 1553 Fragments arranged by size: 2210 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3840 493 Size: 3347 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6049 SphI G_CATG'C Cuts at: 1132 3136 3407 1132 Size: 2004 271 3774 Fragments arranged by size: 3774 2004 271 SspI AAT'ATT Cuts at: 5 52 3973 5 Size: 47 3921 2081 Fragments arranged by size: 3921 2081 47 StuI AGG'CCT Cuts at: 1517 3164 1517 Size: 1647 4402 StyI C'CwwG_G Cuts at: 513 1065 2979 3111 3488 513 Size: 552 1914 132 377 3074 Fragments arranged by size: 3074 1914 552 377 132 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2283 2591 3221 4076 4093 4246 4431 Size: 1255 703 308 630 855 17 153 185 Cuts at: 4431 4876 325 Size: 445 1498 Fragments arranged by size: 1498 1255 855 703 630 445 308 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2937 4295 6007 Size: 274 80 33 51 1443 960 1358 1712 Cuts at: 6007 96 Size: 138 Fragments arranged by size: 1712 1443 1358 960 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2958 2990 6009 6041 2958 Size: 32 3019 32 2966 Fragments arranged by size: 3019 2966 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6049 XbaI T'CTAG_A Cuts at: 3385 3385 Size: 6049 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2426 2426 Size: 6049 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6049 XmaI C'CCGG_G Cuts at: 1870 2193 3374 1870 Size: 323 1181 4545 Fragments arranged by size: 4545 1181 323 XmnI GAAnn'nnTTC Cuts at: 4178 4178 Size: 6049 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3857 276 Size: 53 83 186 936 2323 2468 Fragments arranged by size: 2468 2323 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PpuMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI