(Linear) (Six-base) MAPSORT of: KIEE1423.seq Check: 7326 from: 1 to: 1344 KIEE1423.orf With 182 enzymes: SgfI * July 15, 2010 18:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 25 1344 Size: 25 1319 AccI GT'mk_AC Cuts at: 0 532 1344 Size: 532 812 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 379 1344 Size: 379 965 AflIII A'CryG_T Cuts at: 0 180 1344 Size: 180 1164 AleI CACnn'nnGTG Cuts at: 0 1084 1344 Size: 1084 260 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1165 1197 1234 1266 1344 Size: 1165 32 37 32 78 Fragments arranged by size: 1165 78 37 32 32 ApaI G_GGCC'C Cuts at: 0 741 1167 1344 Size: 741 426 177 Fragments arranged by size: 741 426 177 ApaLI G'TGCA_C Cuts at: 0 384 1213 1344 Size: 384 829 131 Fragments arranged by size: 829 384 131 ApoI r'AATT_y Cuts at: 0 502 1344 Size: 502 842 AvaI C'yCGr_G Cuts at: 0 188 1344 Size: 188 1156 BamHI G'GATC_C Cuts at: 0 362 1344 Size: 362 982 BanI G'GyrC_C Cuts at: 0 777 969 1037 1086 1344 Size: 777 192 68 49 258 Fragments arranged by size: 777 258 192 68 49 BanII G_rGCy'C Cuts at: 0 68 551 741 1167 1344 Size: 68 483 190 426 177 Fragments arranged by size: 483 426 190 177 68 BfrBI ATG'CAT Cuts at: 0 11 1344 Size: 11 1333 BglI GCCn_nnn'nGGC Cuts at: 0 99 1046 1204 1344 Size: 99 947 158 140 Fragments arranged by size: 947 158 140 99 BlpI GC'TnA_GC Cuts at: 0 1132 1344 Size: 1132 212 Bme1580I G_kGCm'C Cuts at: 0 388 433 741 782 974 1042 1167 1217 Size: 388 45 308 41 192 68 125 50 Cuts at: 1217 1344 Size: 127 Fragments arranged by size: 388 308 192 127 125 68 50 45 41 BmrI ACTGGGnnnn_n' Cuts at: 0 104 290 343 1344 Size: 104 186 53 1001 Fragments arranged by size: 1001 186 104 53 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 486 1344 Size: 486 858 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 229 604 1303 1344 Size: 229 375 699 41 Fragments arranged by size: 699 375 229 41 BsaAI yAC'GTr Cuts at: 0 24 183 1344 Size: 24 159 1161 Fragments arranged by size: 1161 159 24 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 953 983 1234 1264 1344 Size: 953 30 251 30 80 Fragments arranged by size: 953 251 80 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 696 1255 1312 1344 Size: 696 559 57 32 Fragments arranged by size: 696 559 57 32 BseYI C'CCAG_C Cuts at: 0 59 516 1344 Size: 59 457 828 Fragments arranged by size: 828 457 59 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1132 1344 Size: 1132 212 BsiEI CG_ry'CG Cuts at: 0 1145 1344 Size: 1145 199 BsiHKAI G_wGCw'C Cuts at: 0 68 388 590 1217 1344 Size: 68 320 202 627 127 Fragments arranged by size: 627 320 202 127 68 BsmI GAATG_Cn' Cuts at: 0 13 1019 1344 Size: 13 1006 325 Fragments arranged by size: 1006 325 13 Bsp1286I G_dGCh'C Cuts at: 0 68 388 433 551 590 741 782 974 Size: 68 320 45 118 39 151 41 192 Cuts at: 974 1042 1167 1217 1344 Size: 68 125 50 127 Fragments arranged by size: 320 192 151 127 125 118 68 68 50 45 41 39 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 25 1344 Size: 25 1319 BsrBI CCG'CTC Cuts at: 0 465 1344 Size: 465 879 BsrDI GCAATG_nn' Cuts at: 0 57 992 1344 Size: 57 935 352 Fragments arranged by size: 935 352 57 BssSI C'ACGA_G Cuts at: 0 847 1344 Size: 847 497 BstAPI GCAn_nnn'nTGC Cuts at: 0 56 1344 Size: 56 1288 BstEII G'GTnAC_C Cuts at: 0 1096 1344 Size: 1096 248 BstXI CCAn_nnnn'nTGG Cuts at: 0 421 903 1344 Size: 421 482 441 Fragments arranged by size: 482 441 421 BstYI r'GATC_y Cuts at: 0 204 230 362 1344 Size: 204 26 132 982 Fragments arranged by size: 982 204 132 26 BtgI C'CryG_G Cuts at: 0 312 539 957 1344 Size: 312 227 418 387 Fragments arranged by size: 418 387 312 227 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1134 1344 Size: 1134 210 EaeI y'GGCC_r Cuts at: 0 806 1103 1344 Size: 806 297 241 Fragments arranged by size: 806 297 241 EarI CTCTTCn'nnn_ Cuts at: 0 262 1344 Size: 262 1082 EcoICRI GAG'CTC Cuts at: 0 66 1344 Size: 66 1278 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 229 379 604 1303 1344 Size: 229 150 225 699 41 Fragments arranged by size: 699 229 225 150 41 EcoNI CCTnn'n_nnAGG Cuts at: 0 1105 1285 1344 Size: 1105 180 59 Fragments arranged by size: 1105 180 59 EcoO109I rG'GnC_Cy Cuts at: 0 737 738 1344 Size: 737 1 606 Fragments arranged by size: 737 606 1 EcoRI G'AATT_C Cuts at: 0 502 1344 Size: 502 842 HaeII r_GCGC'y Cuts at: 0 663 1344 Size: 663 681 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 841 873 1344 Size: 841 32 471 Fragments arranged by size: 841 471 32 HincII GTy'rAC Cuts at: 0 477 984 1344 Size: 477 507 360 Fragments arranged by size: 507 477 360 MscI TGG'CCA Cuts at: 0 808 1105 1344 Size: 808 297 239 Fragments arranged by size: 808 297 239 MslI CAynn'nnrTG Cuts at: 0 1084 1220 1344 Size: 1084 136 124 Fragments arranged by size: 1084 136 124 MspA1I CmG'CkG Cuts at: 0 855 1344 Size: 855 489 NsiI A_TGCA'T Cuts at: 0 13 1344 Size: 13 1331 NspI r_CATG'y Cuts at: 0 1131 1344 Size: 1131 213 PmlI CAC'GTG Cuts at: 0 24 1344 Size: 24 1320 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1234 1266 1344 Size: 1234 32 78 Fragments arranged by size: 1234 78 32 PspOMI G'GGCC_C Cuts at: 0 737 1163 1344 Size: 737 426 181 Fragments arranged by size: 737 426 181 PstI C_TGCA'G Cuts at: 0 834 1344 Size: 834 510 PvuII CAG'CTG Cuts at: 0 855 1344 Size: 855 489 SacI G_AGCT'C Cuts at: 0 68 1344 Size: 68 1276 SapI GCTCTTCn'nnn_ Cuts at: 0 262 1344 Size: 262 1082 SexAI A'CCwGG_T Cuts at: 0 773 1344 Size: 773 571 SfcI C'TryA_G Cuts at: 0 533 830 1344 Size: 533 297 514 Fragments arranged by size: 533 514 297 SmaI CCC'GGG Cuts at: 0 190 1344 Size: 190 1154 SphI G_CATG'C Cuts at: 0 1131 1344 Size: 1131 213 StuI AGG'CCT Cuts at: 0 1159 1344 Size: 1159 185 StyI C'CwwG_G Cuts at: 0 974 1106 1344 Size: 974 132 238 Fragments arranged by size: 974 238 132 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 278 586 1216 1344 Size: 278 308 630 128 Fragments arranged by size: 630 308 278 128 TatI w'GTAC_w Cuts at: 0 932 1344 Size: 932 412 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 953 985 1344 Size: 953 32 359 Fragments arranged by size: 953 359 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 421 1344 Size: 421 923 XmaI C'CCGG_G Cuts at: 0 188 1344 Size: 188 1156 Enzymes that do cut: AarI AccI AcuI AflIII AleI AloI ApaI ApaLI ApoI AvaI BamHI BanI BanII BfrBI BglI BlpI Bme1580I BmrI BplI BpmI BsaAI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BssSI BstAPI BstEII BstXI BstYI BtgI BtgZI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I HincII MscI MslI MspA1I NsiI NspI PmlI PpiI PspOMI PstI PvuII SacI SapI SexAI SfcI SmaI SphI StuI StyI TaqII TatI TstI XcmI XmaI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AgeI AhdI AlfI AlwNI AscI AseI AsiSI AvrII BaeI BbeI BbsI BbvCI BcgI BciVI BclI BglII BmgBI BmtI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsiWI BsmBI BspEI BspHI BsrFI BsrGI BssHII BstBI BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII DrdI EagI EciI EcoRV FalI FseI FspI FspAI HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PacI PasI PciI PflMI PfoI PmeI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XhoI XmnI ZraI