################################## # NO CUT ENZYMES ( 207 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BcnI BfrI BglI BglII BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeII MaeIII MamI MfeI MflI MluI MluNI MroI MscI MslI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIII NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI Sau96I SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI Tsp45I Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 29 enzymes) # ################################### AfaI 254 GTAC GT^AC AvrII 31 CCTAGG C^CTAGG BbvI 172 GCAGC GCAGC(8/12) BfaI 32 CTAG C^TAG BlnI 31 CCTAGG C^CTAGG BpmI 208 CTCCAG CTGGAG(16/14) BsaJI 31 CCTAGG C^C[ACGT][ACGT]GG BsgI 170 GTGCAG GTGCAG(16/14) BsiYI 304 CCAGAAGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 304 CCAGAAGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BspMI 310 GCAGGT ACCTGC(4/8) BsrI 232 ACTGG ACTGG(1/-1) DraI 295 TTTAAA TTT^AAA EcoT14I 31 CCTAGG C^C[AT][AT]GG Fnu4HI 172 GCAGC GC^[ACGT]GC HphI 313 GGTGA GGTGA(8/7) ItaI 172 GCAGC GC^[ACGT]GC MaeI 32 CTAG C^TAG MseI 296 TTAA T^TAA MspA1I 173 CAGCTG C[AC]G^C[GT]G MwoI 54 GCCTCCTGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC PvuII 173 CAGCTG CAG^CTG RsaI 254 GTAC GT^AC SapI 94 GAAGAGC GCTCTTC(1/4) StuI 28 AGGCCT AGG^CCT StyI 31 CCTAGG C^C[AT][AT]GG Tru9I 296 TTAA T^TAA TseI 172 GCAGC G^C[AT]GC TspRI 87 CACTG CA[GC]TG[ACGT][ACGT]^ ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### BclI 144 TGATCA T^GATCA 162 TGATCA BssKI 195 CCTGG ^CC[ACGT]GG 210 CCAGG BstF5I 237 GGATG GGATG(2/0) 241 GGATG BstNI 195 CCTGG CC^[AT]GG 210 CCAGG DdeI 59 CTGAG C^T[ACGT]AG 176 CTGAG EcoRII 195 CCTGG ^CC[AT]GG 210 CCAGG FbaI 144 TGATCA T^GATCA 162 TGATCA FokI 237 GGATG GGATG(9/13) 241 GGATG HaeIII 29 GGCC GG^CC 35 GGCC MnlI 55 CCTC CCTC(7/6) 11 GAGG MvaI 195 CCTGG CC^[AT]GG 210 CCAGG ScrFI 195 CCTGG CC^[ACGT]GG 210 CCAGG ######################################### # 3 OR MORE CUT ENZYMES ( 10 enzymes) # ######################################### AluI DpnII EarI HinfI Ksp632I MboI MboII NdeII Sau3AI TfiI