################################## # NO CUT ENZYMES ( 127 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AhdI AlwNI AocI Aor51HI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BbeI BbvCI BcgI BfaI BfrI BglII BlnI BsaHI BseAI BseRI BsiEI BsiWI BsmBI BsmI Bsp1407I BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstZ17I Bsu36I CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoT22I FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MfeI MluI MroI MseI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflFI PflMI PleI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SexAI SfoI SfuI SnaBI SpeI SphI SspBI SspI StuI SwaI Tru9I Tth111I Van91I XbaI ################################### # ONE CUT ENZYMES ( 61 enzymes) # ################################### AfaI 34 GTAC GT^AC AgeI 625 ACCGGT A^CCGGT Alw44I 569 GTGCAC G^TGCAC AlwI 12 GATCC GGATC(4/5) ApaLI 569 GTGCAC G^TGCAC Asp700I 660 GAACGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 569 GTGCAC G[AT]GC[AT]^C BalI 150 TGGCCA TGG^CCA BbrPI 430 CACGTG CAC^GTG BbsI 664 GTCTTC GAAGAC(2/6) BciVI 125 GTATCC GTATCC(6/5) BclI 434 TGATCA T^GATCA BlpI 84 GCTCAGC GC^T[ACGT]AGC Bpu1102I 84 GCTCAGC GC^T[ACGT]AGC BpuAI 664 GTCTTC GAAGAC(2/6) BsaAI 430 CACGTG [CT]AC^GT[GA] BsaBI 6 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 625 ACCGGT [AT]^CCGG[AT] BsgI 90 CTGCAC GTGCAG(16/14) BsiHKAI 569 GTGCAC G[AT]GC[AT]^C BspDI 64 ATCGAT AT^CGAT BspMI 481 ACCTGC ACCTGC(4/8) BsrFI 625 ACCGGT [GA]^CCGG[CT] BstYI 11 AGATCC [GA]^GATC[CT] CelII 84 GCTCAGC GC^T[ACGT]AGC CfoI 402 GCGC GCG^C Cfr10I 625 ACCGGT [GA]^CCGG[CT] ClaI 64 ATCGAT AT^CGAT DdeI 85 CTCAG C^T[ACGT]AG EcoRV 67 GATATC GAT^ATC FauI 464 CCCGC CCCGC(4/6) FbaI 434 TGATCA T^GATCA HhaI 402 GCGC GCG^C HinP1I 402 GCGC G^CGC MamI 6 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 11 AGATCC [GA]^GATC[CT] MluNI 150 TGGCCA TGG^CCA MscI 150 TGGCCA TGG^CCA MslI 438 CACCAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 87 CAGCTG C[AC]G^C[GT]G PaeR7I 527 CTCGAG C^TCGAG PinAI 625 ACCGGT A^CCGGT PmaCI 430 CACGTG CAC^GTG PmlI 430 CACGTG CAC^GTG PpuMI 160 GGGACCC [GA]G^G[AT]CC[CT] PstI 670 CTGCAG CTGCA^G PvuII 87 CAGCTG CAG^CTG RsaI 34 GTAC GT^AC SbfI 669 CCTGCAGG CCTGCA^GG ScaI 33 AGTACT AGT^ACT SfcI 670 CTGCAG C^T[GA][CT]AG SgrAI 624 CACCGGTG C[GA]^CCGG[CT]G SmaI 120 CCCGGG CCC^GGG Sse8387I 669 CCTGCAGG CCTGCA^GG Tsp509I 454 AATT ^AATT TspRI 468 CAGTG CA[GC]TG[ACGT][ACGT]^ XhoI 527 CTCGAG C^TCGAG XhoII 11 AGATCC [GA]^GATC[CT] XmaCI 120 CCCGGG C^CCGGG XmaI 120 CCCGGG C^CCGGG XmnI 660 GAACGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AciI 420 CCGC CCGC(-3/-1) 465 CCGC AvaI 120 CCCGGG C^[CT]CG[GA]G 527 CTCGAG BanI 489 GGTGCC G^G[CT][GA]CC 709 GGTGCC BbvI 23 GCAGC GCAGC(8/12) 89 GCTGC BsoBI 120 CCCGGG C^[CT]CG[GA]G 527 CTCGAG BsrI 696 ACTGG ACTGG(1/-1) 31 CCAGT BstEII 582 GGTCACC G^GT[ACGT]ACC 618 GGTCACC BstPI 582 GGTCACC G^GT[ACGT]ACC 618 GGTCACC DpnII 12 GATC ^GATC 435 GATC EaeI 150 TGGCCA [CT]^GGCC[GA] 558 TGGCCG EcoO65I 582 GGTCACC G^GT[ACGT]ACC 618 GGTCACC Fnu4HI 23 GCAGC GC^[ACGT]GC 89 GCTGC ItaI 23 GCAGC GC^[ACGT]GC 89 GCTGC MboI 12 GATC ^GATC 435 GATC MboII 9 GAAGA GAAGA(8/7) 665 TCTTC NdeII 12 GATC ^GATC 435 GATC Sau3AI 12 GATC ^GATC 435 GATC SmlI 527 CTCGAG C^T[CT][GA]AG 640 CTCAAG TseI 23 GCAGC G^C[AT]GC 89 GCTGC XcmI 272 CCAAGGGCCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 623 CCACCGGTGGCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 50 enzymes) # ######################################### AluI ApaI AvaII BanII BcnI BglI BmyI BpmI BsaI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BssKI BstF5I BstNI Cac8I Cfr13I DraII DsaI EcoO109I EcoRII EcoT14I FokI HaeIII HapII HinfI HpaII HphI MaeII MaeIII MnlI MspI MvaI MwoI NciI NcoI NlaIII NlaIV Sau96I ScrFI SfaNI SfiI StyI TaqI TfiI Tsp45I TthHB8I