Restriction map for
pFN21AE6351
(Linear) (Six-base) MAPSORT of: KIEE6351.seq Check: 8750 from: 1 to: 1131
KIEE6351.orf
With 182 enzymes: SgfI *
March 16, 2015 11:30 ..
Acc65I G'GTAC_C
Cuts at: 0 524 1131
Size: 524 607
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 173 1131
Size: 173 958
AleI CACnn'nnGTG
Cuts at: 0 679 1131
Size: 679 452
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 133 563 1131
Size: 133 430 568
Fragments arranged by size:
568 430 133
AlwNI CAG_nnn'CTG
Cuts at: 0 741 1131
Size: 741 390
ApoI r'AATT_y
Cuts at: 0 469 1131
Size: 469 662
AvaI C'yCGr_G
Cuts at: 0 85 1131
Size: 85 1046
BamHI G'GATC_C
Cuts at: 0 1056 1131
Size: 1056 75
BanI G'GyrC_C
Cuts at: 0 148 524 673 711 1131
Size: 148 376 149 38 420
Fragments arranged by size:
420 376 149 148 38
BanII G_rGCy'C
Cuts at: 0 266 596 1131
Size: 266 330 535
Fragments arranged by size:
535 330 266
BbeI G_GCGC'C
Cuts at: 0 152 1131
Size: 152 979
BbsI GAAGACnn'nnnn_
Cuts at: 0 651 1131
Size: 651 480
BbvCI CC'TCA_GC
Cuts at: 0 13 532 1131
Size: 13 519 599
Fragments arranged by size:
599 519 13
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1085 1119 1131
Size: 1085 34 12
Fragments arranged by size:
1085 34 12
BlpI GC'TnA_GC
Cuts at: 0 944 1131
Size: 944 187
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 610 1131
Size: 610 521
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 766 1131
Size: 766 365
Bpu10I CC'TnA_GC
Cuts at: 0 13 502 532 1131
Size: 13 489 30 599
Fragments arranged by size:
599 489 30 13
BsaHI Gr'CG_yC
Cuts at: 0 149 518 1131
Size: 149 369 613
Fragments arranged by size:
613 369 149
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 782 812 1131
Size: 782 30 319
Fragments arranged by size:
782 319 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1054 1131
Size: 1054 77
BseYI C'CCAG_C
Cuts at: 0 333 633 780 1131
Size: 333 300 147 351
Fragments arranged by size:
351 333 300 147
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 234 583 1131
Size: 234 349 548
Fragments arranged by size:
548 349 234
BsiEI CG_ry'CG
Cuts at: 0 73 1131
Size: 73 1058
BsiHKAI G_wGCw'C
Cuts at: 0 596 1131
Size: 596 535
BsiWI C'GTAC_G
Cuts at: 0 162 1131
Size: 162 969
BsmI GAATG_Cn'
Cuts at: 0 189 1131
Size: 189 942
Bsp1286I G_dGCh'C
Cuts at: 0 266 596 1131
Size: 266 330 535
Fragments arranged by size:
535 330 266
BspHI T'CATG_A
Cuts at: 0 371 1131
Size: 371 760
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 536 1131
Size: 536 595
BsrDI GCAATG_nn'
Cuts at: 0 815 1131
Size: 815 316
BstAPI GCAn_nnn'nTGC
Cuts at: 0 623 1131
Size: 623 508
BstXI CCAn_nnnn'nTGG
Cuts at: 0 486 684 1131
Size: 486 198 447
Fragments arranged by size:
486 447 198
BstYI r'GATC_y
Cuts at: 0 738 809 1056 1131
Size: 738 71 247 75
Fragments arranged by size:
738 247 75 71
Bsu36I CC'TnA_GG
Cuts at: 0 1052 1131
Size: 1052 79
BtgI C'CryG_G
Cuts at: 0 174 1131
Size: 174 957
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 78 225 1131
Size: 78 147 906
Fragments arranged by size:
906 147 78
BtsI GCAGTG_nn'
Cuts at: 0 39 1073 1131
Size: 39 1034 58
Fragments arranged by size:
1034 58 39
EaeI y'GGCC_r
Cuts at: 0 70 695 1004 1131
Size: 70 625 309 127
Fragments arranged by size:
625 309 127 70
EagI C'GGCC_G
Cuts at: 0 70 1131
Size: 70 1061
EarI CTCTTCn'nnn_
Cuts at: 0 427 446 649 1131
Size: 427 19 203 482
Fragments arranged by size:
482 427 203 19
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 653 1131
Size: 653 478
EcoICRI GAG'CTC
Cuts at: 0 594 1131
Size: 594 537
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 173 766 1131
Size: 173 593 365
Fragments arranged by size:
593 365 173
EcoO109I rG'GnC_Cy
Cuts at: 0 483 1131
Size: 483 648
HaeII r_GCGC'y
Cuts at: 0 130 152 1003 1131
Size: 130 22 851 128
Fragments arranged by size:
851 130 128 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 632 664 782 814 1131
Size: 632 32 118 32 317
Fragments arranged by size:
632 317 118 32 32
KasI G'GCGC_C
Cuts at: 0 148 1131
Size: 148 983
KpnI G_GTAC'C
Cuts at: 0 528 1131
Size: 528 603
MscI TGG'CCA
Cuts at: 0 697 1006 1131
Size: 697 309 125
Fragments arranged by size:
697 309 125
MslI CAynn'nnrTG
Cuts at: 0 679 1131
Size: 679 452
MspA1I CmG'CkG
Cuts at: 0 176 248 417 1063 1131
Size: 176 72 169 646 68
Fragments arranged by size:
646 176 169 72 68
NarI GG'CG_CC
Cuts at: 0 149 1131
Size: 149 982
NotI GC'GGCC_GC
Cuts at: 0 70 1131
Size: 70 1061
NspI r_CATG'y
Cuts at: 0 198 943 1131
Size: 198 745 188
Fragments arranged by size:
745 198 188
PpuMI rG'GwC_Cy
Cuts at: 0 483 1131
Size: 483 648
PvuII CAG'CTG
Cuts at: 0 248 417 1131
Size: 248 169 714
Fragments arranged by size:
714 248 169
SacI G_AGCT'C
Cuts at: 0 596 1131
Size: 596 535
SacII CC_GC'GG
Cuts at: 0 177 1131
Size: 177 954
SapI GCTCTTCn'nnn_
Cuts at: 0 427 1131
Size: 427 704
SexAI A'CCwGG_T
Cuts at: 0 485 962 1131
Size: 485 477 169
Fragments arranged by size:
485 477 169
SfoI GGC'GCC
Cuts at: 0 150 1131
Size: 150 981
SphI G_CATG'C
Cuts at: 0 943 1131
Size: 943 188
SspI AAT'ATT
Cuts at: 0 284 1131
Size: 284 847
StuI AGG'CCT
Cuts at: 0 755 1131
Size: 755 376
StyI C'CwwG_G
Cuts at: 0 479 1007 1131
Size: 479 528 124
Fragments arranged by size:
528 479 124
TatI w'GTAC_w
Cuts at: 0 296 1131
Size: 296 835
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 475 507 1131
Size: 475 32 624
Fragments arranged by size:
624 475 32
Enzymes that do cut:
Acc65I AcuI AleI AlfI AlwNI ApoI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BlpI BplI
BpmI Bpu10I BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI Bsp1286I BspHI BspMI BsrDI BstAPI
BstXI BstYI Bsu36I BtgI BtgZI BtsI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I KasI
KpnI MscI MslI MspA1I NarI NotI NspI PpuMI
PvuII SacI SacII SapI SexAI SfoI SphI SspI
StuI StyI TatI TstI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BciVI BclI BfrBI BglI BglII Bme1580I BmgBI
BmrI BmtI BpuEI BsaI BsaAI BsaBI BsaWI BsmBI
BspEI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII
BstZ17I ClaI CspCI DraI DraIII DrdI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
MfeI MluI MmeI NaeI NcoI NdeI NgoMIV NheI
NruI NsiI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI RsrII SalI SanDI SbfI ScaI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SwaI
TaqII Tth111I XbaI XcmI XhoI XmaI XmnI ZraI