Restriction map for
pFN21AB5978
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# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I AspEI AvaI AvrII BamHI
BbrPI BbsI BcgI BciVI BclI
BfaI BfrI BlnI BpuAI BsaAI
BsaBI BsaI BsaWI BseAI BsiEI
BsiWI BsmI BsoBI Bsp1407I BspEI
BspHI BspLU11I BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstXI
BstZ17I CpoI DraI DraIII EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
EcoRV EcoT22I FbaI FseI HindIII
HpaI KspI MaeI MamI MfeI
MluI MroI MseI MunI MvnI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI ScaI SfiI SfuI SgrAI
SmaI SnaBI SpeI SspBI SspI
SwaI TfiI Tru9I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 1064 GGTACC G^GTACC
AcsI 796 GAATTT [GA]^AATT[CT]
AcyI 283 GGCGCC G[GA]^CG[CT]C
AocI 660 CCTCAGG CC^T[ACGT]AGG
ApaI 369 GGGCCC GGGCC^C
ApoI 796 GAATTT [GA]^AATT[CT]
Asp718I 1064 GGTACC G^GTACC
AspHI 712 GAGCTC G[AT]GC[AT]^C
AspI 1186 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1121 TGCGCA TGC^GCA
BalI 2 TGGCCA TGG^CCA
BbeI 283 GGCGCC GGCGC^C
BglII 1174 AGATCT A^GATCT
BlpI 1234 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1234 GCTGAGC GC^T[ACGT]AGC
BsaHI 283 GGCGCC G[GA]^CG[CT]C
BsiHKAI 712 GAGCTC G[AT]GC[AT]^C
BspDI 454 ATCGAT AT^CGAT
BsrDI 1224 CATTGC GCAATG(2/0)
BstEII 411 GGTCACC G^GT[ACGT]ACC
BstPI 411 GGTCACC G^GT[ACGT]ACC
Bsu36I 660 CCTCAGG CC^T[ACGT]AGG
CelII 1234 GCTGAGC GC^T[ACGT]AGC
ClaI 454 ATCGAT AT^CGAT
DrdI 676 GACAGCATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 962 CCATGG C^C[GA][CT]GG
EaeI 2 TGGCCA [CT]^GGCC[GA]
EarI 1677 GAAGAG CTCTTC(1/4)
Eco81I 660 CCTCAGG CC^T[ACGT]AGG
EcoO65I 411 GGTCACC G^GT[ACGT]ACC
FspI 1121 TGCGCA TGC^GCA
HaeII 283 GGCGCC [GA]GCGC^[CT]
HgaI 104 GCGTC GACGC(5/10)
Hin1I 283 GGCGCC G[GA]^CG[CT]C
KasI 283 GGCGCC G^GCGCC
KpnI 1064 GGTACC GGTAC^C
Ksp632I 1677 GAAGAG CTCTTC(1/4)
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
NarI 283 GGCGCC GG^CGCC
NcoI 962 CCATGG C^CATGG
PflFI 1186 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 712 GAGCTC GAGCT^C
SapI 1677 GAAGAGC GCTCTTC(1/4)
SbfI 1212 CCTGCAGG CCTGCA^GG
SexAI 731 ACCTGGT A^CC[AT]GGT
SfoI 283 GGCGCC GGC^GCC
SmlI 1258 CTCAAG C^T[CT][GA]AG
SphI 722 GCATGC GCATG^C
Sse8387I 1212 CCTGCAGG CCTGCA^GG
StuI 1521 AGGCCT AGG^CCT
Tsp509I 797 AATT ^AATT
Tth111I 1186 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
BglI 86 GCCGCTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
188 GCCTGGGGGGC
BsgI 1419 GTGCAG GTGCAG(16/14)
1465 GTGCAG
BsmBI 105 CGTCTC CGTCTC(1/5)
1476 CGTCTC
BsrBI 87 CCGCTC CCGCTC(-3/-3)
117 CCGCTC
BsrFI 316 GCCGGC [GA]^CCGG[CT]
346 GCCGGC
BsrI 1481 CCAGT ACTGG(1/-1)
1499 CCAGT
BstYI 206 GGATCT [GA]^GATC[CT]
1174 AGATCT
Cfr10I 316 GCCGGC [GA]^CCGG[CT]
346 GCCGGC
EcoT14I 857 CCAAGG C^C[AT][AT]GG
962 CCATGG
HincII 418 GTCAAC GT[CT]^[GA]AC
1450 GTCAAC
HindII 418 GTCAAC GT[CT]^[GA]AC
1450 GTCAAC
HphI 1411 GGTGA GGTGA(8/7)
413 TCACC
MaeII 1190 ACGT A^CGT
1376 ACGT
MboII 810 GAAGA GAAGA(8/7)
1677 GAAGA
MflI 206 GGATCT [GA]^GATC[CT]
1174 AGATCT
MslI 945 CACATCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1455 CATCTCTGTG
NaeI 316 GCCGGC GCC^GGC
346 GCCGGC
NgoMIV 316 GCCGGC G^CCGGC
346 GCCGGC
NspI 148 GCATGT [GA]CATG^[CT]
722 GCATGC
PpuMI 728 AGGACCT [GA]G^G[AT]CC[CT]
1316 AGGACCT
PvuII 225 CAGCTG CAG^CTG
670 CAGCTG
StyI 857 CCAAGG C^C[AT][AT]GG
962 CCATGG
XcmI 1268 CCAAGAACAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1310 CCAAGCAGGACCTGG
XhoII 206 GGATCT [GA]^GATC[CT]
1174 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BanI BanII BbvCI BbvI
BcnI BmyI BpmI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EcoNI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI MaeIII MboI
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TseI Tsp45I
TspRI TthHB8I