Restriction map for
pFN21AB5978
(Linear) (Six-base) MAPSORT of: KIBB5978.seq Check: 9092 from: 1 to: 1692
KIBB5978.orf
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
Acc65I G'GTAC_C
Cuts at: 0 1064 1692
Size: 1064 628
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 81 829 1354 1563 1692
Size: 81 748 525 209 129
Fragments arranged by size:
748 525 209 129 81
AleI CACnn'nnGTG
Cuts at: 0 949 1692
Size: 949 743
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1157 1692
Size: 1157 535
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 622 654 1692
Size: 622 32 1038
Fragments arranged by size:
1038 622 32
AlwNI CAG_nnn'CTG
Cuts at: 0 627 732 1170 1320 1350 1692
Size: 627 105 438 150 30 342
Fragments arranged by size:
627 438 342 150 105 30
ApaI G_GGCC'C
Cuts at: 0 373 1692
Size: 373 1319
ApoI r'AATT_y
Cuts at: 0 796 1692
Size: 796 896
BanI G'GyrC_C
Cuts at: 0 283 604 1064 1495 1692
Size: 283 321 460 431 197
Fragments arranged by size:
460 431 321 283 197
BanII G_rGCy'C
Cuts at: 0 199 373 716 1692
Size: 199 174 343 976
Fragments arranged by size:
976 343 199 174
BbeI G_GCGC'C
Cuts at: 0 287 1692
Size: 287 1405
BbvCI CC'TCA_GC
Cuts at: 0 482 1004 1049 1615 1692
Size: 482 522 45 566 77
Fragments arranged by size:
566 522 482 77 45
BglI GCCn_nnn'nGGC
Cuts at: 0 92 194 1692
Size: 92 102 1498
Fragments arranged by size:
1498 102 92
BglII A'GATC_T
Cuts at: 0 1174 1692
Size: 1174 518
BlpI GC'TnA_GC
Cuts at: 0 1235 1692
Size: 1235 457
Bme1580I G_kGCm'C
Cuts at: 0 373 391 607 1692
Size: 373 18 216 1085
Fragments arranged by size:
1085 373 216 18
BmgBI CAC'GTC
Cuts at: 0 1191 1377 1692
Size: 1191 186 315
Fragments arranged by size:
1191 315 186
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 105 416 571 649 1309 1426 1464 1653
Size: 105 311 155 78 660 117 38 189
Cuts at: 1653 1692
Size: 39
Fragments arranged by size:
660 311 189 155 117 105 78 39
38
Bpu10I CC'TnA_GC
Cuts at: 0 482 1004 1049 1518 1615 1692
Size: 482 522 45 469 97 77
Fragments arranged by size:
522 482 469 97 77 45
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1243 1692
Size: 1243 449
BsaHI Gr'CG_yC
Cuts at: 0 284 1692
Size: 284 1408
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 622 652 1692
Size: 622 30 1040
Fragments arranged by size:
1040 622 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 170 499 1039 1096 1429 1653 1656 1692
Size: 170 329 540 57 333 224 3 36
Fragments arranged by size:
540 333 329 224 170 57 36 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1440 1486 1692
Size: 1440 46 206
Fragments arranged by size:
1440 206 46
BsiHKAI G_wGCw'C
Cuts at: 0 716 1692
Size: 716 976
BsmBI CGTCTCn'nnnn_
Cuts at: 0 111 1482 1692
Size: 111 1371 210
Fragments arranged by size:
1371 210 111
Bsp1286I G_dGCh'C
Cuts at: 0 199 373 391 607 716 1692
Size: 199 174 18 216 109 976
Fragments arranged by size:
976 216 199 174 109 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 455 1080 1127 1220 1692
Size: 455 625 47 93 472
Fragments arranged by size:
625 472 455 93 47
BsrBI CCG'CTC
Cuts at: 0 89 119 1692
Size: 89 30 1573
Fragments arranged by size:
1573 89 30
BsrDI GCAATG_nn'
Cuts at: 0 1223 1692
Size: 1223 469
BsrFI r'CCGG_y
Cuts at: 0 316 346 1692
Size: 316 30 1346
Fragments arranged by size:
1346 316 30
BstEII G'GTnAC_C
Cuts at: 0 411 1692
Size: 411 1281
BstYI r'GATC_y
Cuts at: 0 206 1174 1692
Size: 206 968 518
Fragments arranged by size:
968 518 206
Bsu36I CC'TnA_GG
Cuts at: 0 661 1692
Size: 661 1031
BtgI C'CryG_G
Cuts at: 0 962 1692
Size: 962 730
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 984 1692
Size: 984 708
BtsI GCAGTG_nn'
Cuts at: 0 135 1207 1518 1560 1596 1692
Size: 135 1072 311 42 36 96
Fragments arranged by size:
1072 311 135 96 42 36
ClaI AT'CG_AT
Cuts at: 0 455 1692
Size: 455 1237
DrdI GACnn_nn'nnGTC
Cuts at: 0 682 1692
Size: 682 1010
EaeI y'GGCC_r
Cuts at: 0 2 1692
Size: 2 1690
EarI CTCTTCn'nnn_
Cuts at: 0 1672 1692
Size: 1672 20
EcoICRI GAG'CTC
Cuts at: 0 714 1692
Size: 714 978
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 81 105 416 571 649 829 1309 1354
Size: 81 24 311 155 78 180 480 45
Cuts at: 1354 1426 1464 1563 1653 1692
Size: 72 38 99 90 39
Fragments arranged by size:
480 311 180 155 99 90 81 78
72 45 39 38 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 592 736 1303 1671 1692
Size: 592 144 567 368 21
Fragments arranged by size:
592 567 368 144 21
EcoO109I rG'GnC_Cy
Cuts at: 0 369 370 729 1317 1692
Size: 369 1 359 588 375
Fragments arranged by size:
588 375 369 359 1
FspI TGC'GCA
Cuts at: 0 1123 1692
Size: 1123 569
HaeII r_GCGC'y
Cuts at: 0 287 1692
Size: 287 1405
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1042 1074 1166 1198 1692
Size: 1042 32 92 32 494
Fragments arranged by size:
1042 494 92 32 32
HincII GTy'rAC
Cuts at: 0 420 1452 1692
Size: 420 1032 240
Fragments arranged by size:
1032 420 240
KasI G'GCGC_C
Cuts at: 0 283 1692
Size: 283 1409
KpnI G_GTAC'C
Cuts at: 0 1068 1692
Size: 1068 624
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1422 1548 1605 1692
Size: 1422 126 57 87
Fragments arranged by size:
1422 126 87 57
MscI TGG'CCA
Cuts at: 0 4 1692
Size: 4 1688
MslI CAynn'nnrTG
Cuts at: 0 949 1459 1692
Size: 949 510 233
Fragments arranged by size:
949 510 233
MspA1I CmG'CkG
Cuts at: 0 227 471 672 1692
Size: 227 244 201 1020
Fragments arranged by size:
1020 244 227 201
NaeI GCC'GGC
Cuts at: 0 318 348 1692
Size: 318 30 1344
Fragments arranged by size:
1344 318 30
NarI GG'CG_CC
Cuts at: 0 284 1692
Size: 284 1408
NcoI C'CATG_G
Cuts at: 0 962 1692
Size: 962 730
NgoMIV G'CCGG_C
Cuts at: 0 316 346 1692
Size: 316 30 1346
Fragments arranged by size:
1346 316 30
NspI r_CATG'y
Cuts at: 0 152 726 1692
Size: 152 574 966
Fragments arranged by size:
966 574 152
PasI CC'CwG_GG
Cuts at: 0 76 1692
Size: 76 1616
PfoI T'CCnGG_A
Cuts at: 0 548 1692
Size: 548 1144
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 622 654 1692
Size: 622 32 1038
Fragments arranged by size:
1038 622 32
PpuMI rG'GwC_Cy
Cuts at: 0 729 1317 1692
Size: 729 588 375
Fragments arranged by size:
729 588 375
PspOMI G'GGCC_C
Cuts at: 0 369 1692
Size: 369 1323
PstI C_TGCA'G
Cuts at: 0 596 1091 1217 1304 1692
Size: 596 495 126 87 388
Fragments arranged by size:
596 495 388 126 87
PvuII CAG'CTG
Cuts at: 0 227 672 1692
Size: 227 445 1020
Fragments arranged by size:
1020 445 227
SacI G_AGCT'C
Cuts at: 0 716 1692
Size: 716 976
SapI GCTCTTCn'nnn_
Cuts at: 0 1672 1692
Size: 1672 20
SbfI CC_TGCA'GG
Cuts at: 0 1217 1692
Size: 1217 475
SexAI A'CCwGG_T
Cuts at: 0 731 1692
Size: 731 961
SfcI C'TryA_G
Cuts at: 0 592 1087 1213 1300 1692
Size: 592 495 126 87 392
Fragments arranged by size:
592 495 392 126 87
SfoI GGC'GCC
Cuts at: 0 285 1692
Size: 285 1407
SmlI C'TyrA_G
Cuts at: 0 1258 1692
Size: 1258 434
SphI G_CATG'C
Cuts at: 0 726 1692
Size: 726 966
StuI AGG'CCT
Cuts at: 0 1523 1692
Size: 1523 169
StyI C'CwwG_G
Cuts at: 0 857 962 1692
Size: 857 105 730
Fragments arranged by size:
857 730 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 130 325 1516 1692
Size: 130 195 1191 176
Fragments arranged by size:
1191 195 176 130
TatI w'GTAC_w
Cuts at: 0 647 1649 1692
Size: 647 1002 43
Fragments arranged by size:
1002 647 43
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 565 597 1692
Size: 565 32 1095
Fragments arranged by size:
1095 565 32
Tth111I GACn'n_nGTC
Cuts at: 0 1189 1692
Size: 1189 503
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1275 1317 1692
Size: 1275 42 375
Fragments arranged by size:
1275 375 42
Enzymes that do cut:
Acc65I AcuI AleI AlfI AloI AlwNI ApaI ApoI
BanI BanII BbeI BbvCI BglI BglII BlpI Bme1580I
BmgBI BpmI Bpu10I BpuEI BsaHI BsaXI BseRI BsgI
BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI DrdI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I
HincII KasI KpnI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PfoI PpiI PpuMI
PspOMI PstI PvuII SacI SapI SbfI SexAI SfcI
SfoI SmlI SphI StuI StyI TaqII TatI TstI
Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BbsI BcgI BciVI BclI BfrBI BmrI BmtI
BplI BsaI BsaAI BsaBI BsaWI BseYI BsiEI BsiWI
BsmI BspEI BspHI BsrGI BssHII BssSI BstAPI BstBI
BstXI BstZ17I CspCI DraI DraIII EagI EciI EcoRI
EcoRV FalI FseI FspAI HindIII HpaI MfeI MluI
NdeI NheI NotI NruI NsiI PacI PciI PflMI
PmeI PmlI PshAI PsiI PspXI PsrI PvuI RsrII
SacII SalI SanDI ScaI SfiI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI SwaI XbaI XhoI XmaI
XmnI ZraI